Incidental Mutation 'R5892:Nat3'
ID457248
Institutional Source Beutler Lab
Gene Symbol Nat3
Ensembl Gene ENSMUSG00000056426
Gene NameN-acetyltransferase 3
Synonyms
MMRRC Submission 044093-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5892 (G1)
Quality Score225
Status Validated
Chromosome8
Chromosomal Location67523854-67548627 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 67547938 bp
ZygosityHeterozygous
Amino Acid Change Asparagine to Lysine at position 156 (N156K)
Ref Sequence ENSEMBL: ENSMUSP00000069359 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000070514]
Predicted Effect probably benign
Transcript: ENSMUST00000070514
AA Change: N156K

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000069359
Gene: ENSMUSG00000056426
AA Change: N156K

DomainStartEndE-ValueType
Pfam:Acetyltransf_2 20 280 3.6e-91 PFAM
Meta Mutation Damage Score 0.1164 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.6%
  • 20x: 92.6%
Validation Efficiency 98% (56/57)
MGI Phenotype PHENOTYPE: Mice homozygous for a null mutation are viable and fertile with no gross abnormalities. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930524J08Rik G T 5: 99,979,265 probably benign Het
Abcb11 C T 2: 69,261,500 A893T probably damaging Het
Adtrp A T 13: 41,828,206 N30K probably benign Het
Alms1 T A 6: 85,620,903 S904T probably damaging Het
Cep170 T A 1: 176,755,387 probably null Het
Cntnap3 A G 13: 64,799,180 V145A probably damaging Het
Dclre1a T C 19: 56,547,140 E4G probably benign Het
Depdc1a G C 3: 159,526,669 A686P probably damaging Het
Dnah7b T C 1: 46,337,593 probably null Het
Enox1 A G 14: 77,486,017 probably benign Het
Fam107b A G 2: 3,778,564 E268G probably damaging Het
Fer1l6 A G 15: 58,564,068 S437G probably benign Het
Flnb C A 14: 7,907,183 T1252K probably damaging Het
Fubp1 A G 3: 152,218,314 probably benign Het
Gm20402 G A 3: 52,268,949 A50T probably benign Het
Gpr137b T C 13: 13,359,406 Y355C probably damaging Het
Gzmk C T 13: 113,173,922 probably null Het
Ino80 A T 2: 119,439,547 probably benign Het
Ints8 T C 4: 11,223,813 T677A probably damaging Het
Kat6a A G 8: 22,938,289 E1220G probably damaging Het
Lrfn5 A G 12: 61,843,418 T498A probably damaging Het
Lrp2 T A 2: 69,442,776 T4043S probably benign Het
Malrd1 A G 2: 15,614,267 N314S probably benign Het
Mfsd1 A G 3: 67,589,829 probably null Het
Muc1 C T 3: 89,230,993 P381S probably benign Het
Myh2 A T 11: 67,185,176 K730* probably null Het
Nelfe T C 17: 34,854,669 probably benign Het
Nlrp1a A T 11: 71,099,645 L927Q probably damaging Het
P4ha2 A G 11: 54,120,188 Y343C probably damaging Het
Pclo A T 5: 14,521,171 Q190L probably damaging Het
Pde8a A G 7: 81,295,691 N183S probably damaging Het
Plekha2 C T 8: 25,052,365 V278I probably benign Het
Plekha6 G C 1: 133,272,307 R208P possibly damaging Het
Plxnb1 T C 9: 109,111,707 L1550P probably damaging Het
Ppox C T 1: 171,277,461 V385I probably damaging Het
Prdm16 T C 4: 154,323,259 D1170G possibly damaging Het
Prmt5 A T 14: 54,509,911 S470T probably damaging Het
Robo2 T A 16: 73,895,780 probably benign Het
Siglecg G A 7: 43,412,204 probably benign Het
Slc30a5 T C 13: 100,813,302 N367S probably damaging Het
Tex29 C T 8: 11,854,288 probably benign Het
Tmem117 T A 15: 94,638,139 V18E probably damaging Het
Tmtc2 C T 10: 105,413,505 M122I probably benign Het
Tpr T A 1: 150,407,400 N287K probably benign Het
Unc93b1 A T 19: 3,943,632 D358V probably damaging Het
Vrk2 A T 11: 26,534,372 probably benign Het
Wdr24 A T 17: 25,827,986 Q671L probably benign Het
Zfp110 C T 7: 12,848,478 T351I probably benign Het
Zfp963 T C 8: 69,743,377 D142G probably benign Het
Other mutations in Nat3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01912:Nat3 APN 8 67547746 nonsense probably null
IGL03277:Nat3 APN 8 67547689 missense probably benign 0.11
IGL02802:Nat3 UTSW 8 67547508 missense probably benign 0.08
R0266:Nat3 UTSW 8 67547780 missense probably benign
R0842:Nat3 UTSW 8 67547997 missense probably benign 0.01
R5245:Nat3 UTSW 8 67548180 missense probably benign 0.09
R5461:Nat3 UTSW 8 67547862 missense probably damaging 1.00
R6371:Nat3 UTSW 8 67524179 splice site probably null
R7523:Nat3 UTSW 8 67547574 missense probably damaging 0.99
R7587:Nat3 UTSW 8 67547574 missense probably damaging 0.99
R7677:Nat3 UTSW 8 67547487 missense probably damaging 1.00
R8052:Nat3 UTSW 8 67547826 missense not run
X0067:Nat3 UTSW 8 67548334 missense probably benign 0.09
Z1176:Nat3 UTSW 8 67547814 missense not run
Predicted Primers PCR Primer
(F):5'- TATGTCCCTTCAGCCTGCAAG -3'
(R):5'- CCGTCTTTGGTATGTAGGGAAC -3'

Sequencing Primer
(F):5'- ATGTCCCTTCAGCCTGCAAGTATAG -3'
(R):5'- CATCACAGATGTTCGAGATACCTGG -3'
Posted On2017-02-15