Mutagenetix > Incidental Mutation

Incidental Mutation 'R5894:Slc44a5'

457363

Institutional Source

Beutler Lab

Gene Symbol

Slc44a5

Ensembl Gene

ENSMUSG00000028360

Gene Name

solute carrier family 44, member 5

Synonyms

LOC242259

MMRRC Submission

043238-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R5894 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

153679073-153977359 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 153962210 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 381 (Y381C)

Ref Sequence

ENSEMBL: ENSMUSP00000087394 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089948]

AlphaFold

Q5RJI2

Predicted Effect

probably damaging
Transcript: ENSMUST00000089948
AA Change: Y381C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000087394
Gene: ENSMUSG00000028360
AA Change: Y381C

Domain	Start	End	E-Value	Type
transmembrane domain	33	55	N/A	INTRINSIC
transmembrane domain	234	256	N/A	INTRINSIC
transmembrane domain	263	285	N/A	INTRINSIC
Pfam:Choline_transpo	323	683	6.3e-114	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144677

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.5%
20x: 92.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503B20Rik	T	A	3: 146,356,680 (GRCm39)	E76V	probably benign	Het
Adcy2	A	T	13: 68,773,971 (GRCm39)	I1024N	probably damaging	Het
Agr2	A	C	12: 36,045,509 (GRCm39)		probably benign	Het
Alpk2	T	A	18: 65,414,143 (GRCm39)	H1991L	probably damaging	Het
Amotl2	T	A	9: 102,602,371 (GRCm39)	M448K	possibly damaging	Het
Ankhd1	T	A	18: 36,780,577 (GRCm39)	F1876L	probably damaging	Het
Arl14	T	C	3: 69,130,009 (GRCm39)	V52A	probably benign	Het
Atp6v1b2	T	A	8: 69,560,218 (GRCm39)		probably null	Het
Bmal2	A	G	6: 146,724,732 (GRCm39)	T409A	possibly damaging	Het
Bpifb4	G	A	2: 153,782,852 (GRCm39)	R19H	possibly damaging	Het
Cdh9	T	A	15: 16,832,186 (GRCm39)	L358I	possibly damaging	Het
Ces2h	T	G	8: 105,745,658 (GRCm39)	I460M	probably benign	Het
Cog2	T	C	8: 125,272,006 (GRCm39)	Y507H	probably benign	Het
Crlf3	T	A	11: 79,948,678 (GRCm39)	R256W	probably damaging	Het
Csdc2	T	A	15: 81,832,881 (GRCm39)	F96I	probably damaging	Het
Csk	A	C	9: 57,535,958 (GRCm39)	I264S	probably damaging	Het
Cyp2c29	G	C	19: 39,318,833 (GRCm39)	A438P	possibly damaging	Het
Ddx43	G	A	9: 78,324,016 (GRCm39)	G449D	probably damaging	Het
Fbxw18	G	T	9: 109,529,235 (GRCm39)	A106E	possibly damaging	Het
Fchsd2	C	T	7: 100,840,959 (GRCm39)	T156I	probably benign	Het
Frs2	T	A	10: 116,917,011 (GRCm39)		probably benign	Het
Gm57858	C	T	3: 36,074,124 (GRCm39)	E342K	possibly damaging	Het
Grid2ip	A	T	5: 143,374,666 (GRCm39)	T922S	probably damaging	Het
Grm1	A	G	10: 10,955,999 (GRCm39)	L95P	probably damaging	Het
Ift140	T	C	17: 25,252,893 (GRCm39)	V348A	possibly damaging	Het
Insr	A	G	8: 3,224,869 (GRCm39)	S200P	possibly damaging	Het
Ints12	A	T	3: 132,804,319 (GRCm39)	D102V	probably damaging	Het
Kank1	A	G	19: 25,401,564 (GRCm39)	D1057G	probably damaging	Het
Kng1	T	A	16: 22,892,113 (GRCm39)	D225E	probably benign	Het
Lama1	A	T	17: 68,086,042 (GRCm39)		probably null	Het
Lpin2	A	G	17: 71,553,929 (GRCm39)	D882G	probably benign	Het
Lrp1b	A	T	2: 41,388,233 (GRCm39)	F464Y	probably benign	Het
Musk	G	A	4: 58,373,583 (GRCm39)	C836Y	probably damaging	Het
Mx1	C	T	16: 97,255,406 (GRCm39)	D216N	probably damaging	Het
Ndrg3	A	T	2: 156,770,698 (GRCm39)	N350K	probably benign	Het
Oas3	G	A	5: 120,895,019 (GRCm39)	P990L	probably damaging	Het
Or4c111	T	A	2: 88,844,399 (GRCm39)	N3I	probably damaging	Het
Or4c12b	T	C	2: 89,647,557 (GRCm39)	S296P	possibly damaging	Het
Or6c2	A	G	10: 129,362,357 (GRCm39)	D87G	probably damaging	Het
Otoa	T	C	7: 120,721,092 (GRCm39)	L369P	probably damaging	Het
Plcg2	A	G	8: 118,231,088 (GRCm39)	T57A	probably damaging	Het
Prune2	A	T	19: 17,098,755 (GRCm39)	T1420S	possibly damaging	Het
Ptpdc1	A	G	13: 48,743,798 (GRCm39)	F201S	probably damaging	Het
Rnf214	A	G	9: 45,777,916 (GRCm39)	V630A	probably damaging	Het
Rxrb	T	A	17: 34,254,718 (GRCm39)	I181N	probably damaging	Het
Scrib	T	C	15: 75,939,581 (GRCm39)	N69S	probably damaging	Het
Scyl2	A	G	10: 89,476,681 (GRCm39)	S815P	probably benign	Het
Sgcd	A	G	11: 47,246,028 (GRCm39)	V58A	probably damaging	Het
Slc13a3	C	T	2: 165,266,543 (GRCm39)	V374I	probably benign	Het
Slc22a4	A	T	11: 53,888,341 (GRCm39)	I229N	probably benign	Het
Sohlh1	A	G	2: 25,734,679 (GRCm39)	S205P	possibly damaging	Het
Spata20	T	C	11: 94,374,444 (GRCm39)	M308V	probably damaging	Het
Stag3	T	G	5: 138,297,100 (GRCm39)	I550R	probably damaging	Het
Tac2	A	G	10: 127,561,971 (GRCm39)	E25G	possibly damaging	Het
Tars2	T	C	3: 95,654,964 (GRCm39)		probably null	Het
Tefm	G	A	11: 80,031,057 (GRCm39)	R60C	probably damaging	Het
Trem3	T	C	17: 48,565,483 (GRCm39)	V179A	probably benign	Het
Trim37	G	T	11: 87,092,266 (GRCm39)	D692Y	probably damaging	Het
Trpc4ap	T	C	2: 155,508,133 (GRCm39)	T173A	probably benign	Het
Unc13c	C	T	9: 73,600,486 (GRCm39)		probably null	Het
Usp18	A	G	6: 121,238,456 (GRCm39)	K201R	probably benign	Het
Usp35	T	A	7: 96,962,284 (GRCm39)	Y524F	probably damaging	Het
Usp53	A	G	3: 122,752,734 (GRCm39)	F208L	probably damaging	Het
Vmn1r80	A	T	7: 11,927,654 (GRCm39)	I255F	probably damaging	Het
Vstm2a	A	T	11: 16,211,483 (GRCm39)	I98F	probably benign	Het
Wdfy4	T	A	14: 32,855,317 (GRCm39)	I766F	possibly damaging	Het
Wdr17	T	A	8: 55,149,335 (GRCm39)	Y55F	probably damaging	Het
Xpot	T	C	10: 121,449,551 (GRCm39)	K172R	probably damaging	Het

Other mutations in Slc44a5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00321:Slc44a5	APN	3	153,968,576 (GRCm39)	missense	probably damaging	1.00
IGL02187:Slc44a5	APN	3	153,968,554 (GRCm39)	missense	probably benign	0.00
IGL02219:Slc44a5	APN	3	153,944,796 (GRCm39)	missense	possibly damaging	0.76
IGL02220:Slc44a5	APN	3	153,956,608 (GRCm39)	missense	possibly damaging	0.94
IGL02314:Slc44a5	APN	3	153,962,156 (GRCm39)	missense	probably damaging	1.00
IGL02471:Slc44a5	APN	3	153,962,213 (GRCm39)	missense	probably damaging	1.00
IGL02869:Slc44a5	APN	3	153,956,651 (GRCm39)	missense	probably damaging	0.97
IGL03117:Slc44a5	APN	3	153,956,714 (GRCm39)	missense	probably benign	0.15
IGL03144:Slc44a5	APN	3	153,949,177 (GRCm39)	missense	possibly damaging	0.78
PIT4486001:Slc44a5	UTSW	3	153,964,659 (GRCm39)	missense	possibly damaging	0.50
R0026:Slc44a5	UTSW	3	153,945,907 (GRCm39)	splice site	probably benign
R0190:Slc44a5	UTSW	3	153,944,755 (GRCm39)	missense	probably null	0.00
R0306:Slc44a5	UTSW	3	153,975,638 (GRCm39)	missense	probably damaging	1.00
R0591:Slc44a5	UTSW	3	153,939,782 (GRCm39)	splice site	probably benign
R0744:Slc44a5	UTSW	3	153,971,111 (GRCm39)	missense	probably damaging	1.00
R0833:Slc44a5	UTSW	3	153,971,111 (GRCm39)	missense	probably damaging	1.00
R0919:Slc44a5	UTSW	3	153,949,223 (GRCm39)	missense	probably damaging	1.00
R1170:Slc44a5	UTSW	3	153,963,357 (GRCm39)	splice site	probably null
R1370:Slc44a5	UTSW	3	153,948,796 (GRCm39)	missense	probably benign	0.26
R1613:Slc44a5	UTSW	3	153,963,351 (GRCm39)	splice site	probably null
R1713:Slc44a5	UTSW	3	153,944,743 (GRCm39)	missense	probably damaging	0.97
R1999:Slc44a5	UTSW	3	153,964,130 (GRCm39)	missense	possibly damaging	0.61
R2143:Slc44a5	UTSW	3	153,964,086 (GRCm39)	missense	probably benign	0.01
R2293:Slc44a5	UTSW	3	153,945,934 (GRCm39)	missense	probably benign	0.01
R2367:Slc44a5	UTSW	3	153,953,446 (GRCm39)	missense	possibly damaging	0.94
R3842:Slc44a5	UTSW	3	153,967,031 (GRCm39)	splice site	probably benign
R3953:Slc44a5	UTSW	3	153,877,209 (GRCm39)	missense	probably benign	0.03
R4163:Slc44a5	UTSW	3	153,967,010 (GRCm39)	missense	possibly damaging	0.94
R4509:Slc44a5	UTSW	3	153,939,710 (GRCm39)	missense	probably damaging	1.00
R4657:Slc44a5	UTSW	3	153,962,221 (GRCm39)	missense	possibly damaging	0.81
R4936:Slc44a5	UTSW	3	153,959,353 (GRCm39)	missense	probably damaging	1.00
R4937:Slc44a5	UTSW	3	153,949,252 (GRCm39)	critical splice donor site	probably null
R5083:Slc44a5	UTSW	3	153,953,424 (GRCm39)	missense	probably benign	0.31
R5257:Slc44a5	UTSW	3	153,948,760 (GRCm39)	missense	probably damaging	1.00
R5580:Slc44a5	UTSW	3	153,966,922 (GRCm39)	missense	probably benign	0.03
R5586:Slc44a5	UTSW	3	153,975,802 (GRCm39)	utr 3 prime	probably benign
R5856:Slc44a5	UTSW	3	153,964,029 (GRCm39)	missense	possibly damaging	0.78
R6228:Slc44a5	UTSW	3	153,944,800 (GRCm39)	missense	probably benign	0.00
R6248:Slc44a5	UTSW	3	153,969,678 (GRCm39)	missense	possibly damaging	0.93
R6413:Slc44a5	UTSW	3	153,963,387 (GRCm39)	missense	probably benign	0.21
R6454:Slc44a5	UTSW	3	153,948,796 (GRCm39)	missense	probably benign	0.26
R6594:Slc44a5	UTSW	3	153,964,660 (GRCm39)	missense	possibly damaging	0.85
R6761:Slc44a5	UTSW	3	153,945,714 (GRCm39)	splice site	probably null
R6931:Slc44a5	UTSW	3	153,964,143 (GRCm39)	missense	probably benign	0.03
R7027:Slc44a5	UTSW	3	153,959,356 (GRCm39)	missense	probably benign	0.00
R7566:Slc44a5	UTSW	3	153,975,626 (GRCm39)	missense	probably damaging	1.00
R7688:Slc44a5	UTSW	3	153,679,437 (GRCm39)	critical splice donor site	probably null
R8011:Slc44a5	UTSW	3	153,953,447 (GRCm39)	missense	possibly damaging	0.71
R8085:Slc44a5	UTSW	3	153,928,414 (GRCm39)	missense	probably damaging	1.00
R8460:Slc44a5	UTSW	3	153,975,667 (GRCm39)	missense	probably benign	0.00
R8765:Slc44a5	UTSW	3	153,968,561 (GRCm39)	missense	probably damaging	0.99
R9154:Slc44a5	UTSW	3	153,953,373 (GRCm39)	missense	probably benign	0.08
R9291:Slc44a5	UTSW	3	153,962,218 (GRCm39)	missense	possibly damaging	0.82
R9328:Slc44a5	UTSW	3	153,964,678 (GRCm39)	missense	probably benign	0.37
R9647:Slc44a5	UTSW	3	153,953,370 (GRCm39)	missense	possibly damaging	0.89
R9666:Slc44a5	UTSW	3	153,945,926 (GRCm39)	missense	probably benign	0.03
R9695:Slc44a5	UTSW	3	153,956,588 (GRCm39)	missense	probably damaging	0.99
R9758:Slc44a5	UTSW	3	153,959,322 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAACCTAAGGCTTGCTTCTGC -3'
(R):5'- TCTGTTCCTGAGCAGCAAG -3'

Sequencing Primer
(F):5'- GCTTCTGCTTCTTAACGGATAAAC -3'
(R):5'- TTCCTGAGCAGCAAGGTGGAC -3'

Posted On

2017-02-15