Mutagenetix > Incidental Mutation

Incidental Mutation 'R5897:Vmn2r4'

457574

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r4

Ensembl Gene

ENSMUSG00000092049

Gene Name

vomeronasal 2, receptor 4

Synonyms

EG637053

MMRRC Submission

044096-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R5897 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

64295982-64322741 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 64322687 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Stop codon at position 11 (G11*)

Ref Sequence

ENSEMBL: ENSMUSP00000135228 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000175724]

AlphaFold

K7N784

Predicted Effect

probably null
Transcript: ENSMUST00000175724
AA Change: G11*

SMART Domains

Protein: ENSMUSP00000135228
Gene: ENSMUSG00000092049
AA Change: G11*

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	2.3e-75	PFAM
Pfam:NCD3G	547	600	4.7e-17	PFAM
Pfam:7tm_3	633	867	8.2e-47	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.4%
10x: 97.0%
20x: 90.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi3bp	A	G	16: 56,425,032 (GRCm39)	T489A	possibly damaging	Het
Adam3	T	C	8: 25,187,244 (GRCm39)	T416A	probably benign	Het
Agbl3	A	G	6: 34,780,508 (GRCm39)	I494V	probably benign	Het
Akap9	T	C	5: 4,127,904 (GRCm39)	S3747P	probably benign	Het
Arhgef18	G	T	8: 3,489,682 (GRCm39)	R338L	probably damaging	Het
Atg16l1	G	T	1: 87,713,719 (GRCm39)		probably null	Het
BC024139	A	G	15: 76,010,339 (GRCm39)	S56P	possibly damaging	Het
Ccdc121	T	C	5: 31,643,308 (GRCm39)		probably benign	Het
Ccdc175	T	A	12: 72,206,578 (GRCm39)	I223L	probably benign	Het
Cdcp3	A	T	7: 130,798,280 (GRCm39)		probably null	Het
Cep76	T	A	18: 67,771,398 (GRCm39)	Q78L	probably benign	Het
Cyp20a1	A	C	1: 60,392,220 (GRCm39)	N92T	probably damaging	Het
Cyp2c68	T	C	19: 39,700,975 (GRCm39)	D281G	probably benign	Het
Cyp2j12	T	C	4: 95,990,279 (GRCm39)	N429S	probably damaging	Het
Dchs2	G	A	3: 83,192,717 (GRCm39)	V1721I	possibly damaging	Het
Elovl4	G	A	9: 83,672,157 (GRCm39)	T54M	possibly damaging	Het
Fam13b	A	G	18: 34,587,134 (GRCm39)	V542A	possibly damaging	Het
Gje1	A	T	10: 14,592,467 (GRCm39)	I105N	probably damaging	Het
Grm4	G	T	17: 27,654,137 (GRCm39)	F349L	probably benign	Het
Hsd17b3	A	G	13: 64,236,799 (GRCm39)		probably null	Het
Khdrbs1	A	T	4: 129,614,448 (GRCm39)	D368E	probably benign	Het
Klra10	T	A	6: 130,258,792 (GRCm39)	R32*	probably null	Het
Lrrc7	T	A	3: 157,869,990 (GRCm39)	I644F	probably damaging	Het
Or2i1	A	T	17: 37,508,151 (GRCm39)	L161Q	probably damaging	Het
Or2t1	G	T	14: 14,328,120 (GRCm38)	G3V	probably benign	Het
Otogl	G	A	10: 107,612,978 (GRCm39)		silent	Het
Pbx3	A	T	2: 34,261,920 (GRCm39)	S5T	probably benign	Het
Pcdhb13	T	A	18: 37,576,264 (GRCm39)	V214E	probably benign	Het
Pkd1l1	T	C	11: 8,829,176 (GRCm39)	D1022G	probably benign	Het
Pla2g4a	T	A	1: 149,740,899 (GRCm39)	K380I	probably damaging	Het
Prl3b1	A	G	13: 27,429,858 (GRCm39)	T99A	probably benign	Het
Prr12	C	T	7: 44,692,808 (GRCm39)	V1575I	probably damaging	Het
Ptpn5	C	T	7: 46,729,262 (GRCm39)	V451I	probably benign	Het
Rab1a	C	T	11: 20,168,867 (GRCm39)	R51*	probably null	Het
Skint9	A	G	4: 112,271,113 (GRCm39)	I99T	possibly damaging	Het
Tbc1d8	A	G	1: 39,431,190 (GRCm39)	V394A	possibly damaging	Het
Tcf20	A	T	15: 82,735,984 (GRCm39)	C1822*	probably null	Het
Tdrd6	T	A	17: 43,935,768 (GRCm39)	D1760V	probably damaging	Het
Tinag	C	A	9: 76,952,726 (GRCm39)	C86F	probably damaging	Het
Tmtc2	A	T	10: 105,249,459 (GRCm39)	N91K	probably damaging	Het
Tmub1	G	A	5: 24,651,925 (GRCm39)	T13M	probably benign	Het
Txn2	G	A	15: 77,808,726 (GRCm39)	A135V	probably benign	Het
Ush2a	G	A	1: 188,553,935 (GRCm39)	G3475R	probably damaging	Het
Usp38	T	C	8: 81,732,082 (GRCm39)	D276G	probably benign	Het
Vax1	T	A	19: 59,158,233 (GRCm39)	I77F	unknown	Het
Vmn2r108	C	T	17: 20,691,580 (GRCm39)	M314I	probably benign	Het
Zcchc14	T	C	8: 122,331,899 (GRCm39)		probably benign	Het

Other mutations in Vmn2r4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Vmn2r4	APN	3	64,317,200 (GRCm39)	splice site	probably null
IGL01448:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01452:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01454:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01456:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01463:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01467:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01468:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01470:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01476:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01481:Vmn2r4	APN	3	64,313,816 (GRCm39)	missense	probably damaging	0.99
IGL01534:Vmn2r4	APN	3	64,313,844 (GRCm39)	missense	probably damaging	1.00
IGL01636:Vmn2r4	APN	3	64,313,657 (GRCm39)	missense	probably benign	0.21
IGL01879:Vmn2r4	APN	3	64,298,431 (GRCm39)	missense	probably damaging	1.00
IGL02147:Vmn2r4	APN	3	64,305,782 (GRCm39)	splice site	probably benign
IGL02276:Vmn2r4	APN	3	64,313,877 (GRCm39)	missense	possibly damaging	0.95
IGL02432:Vmn2r4	APN	3	64,313,821 (GRCm39)	missense	probably benign	0.38
IGL02533:Vmn2r4	APN	3	64,305,840 (GRCm39)	nonsense	probably null
IGL02655:Vmn2r4	APN	3	64,305,886 (GRCm39)	missense	probably damaging	0.97
IGL02666:Vmn2r4	APN	3	64,296,433 (GRCm39)	missense	probably benign	0.10
IGL02902:Vmn2r4	APN	3	64,314,337 (GRCm39)	missense	probably benign	0.22
IGL03189:Vmn2r4	APN	3	64,296,589 (GRCm39)	missense	possibly damaging	0.89
IGL03250:Vmn2r4	APN	3	64,314,063 (GRCm39)	missense	probably damaging	1.00
IGL03271:Vmn2r4	APN	3	64,305,850 (GRCm39)	missense	probably benign	0.01
R0310:Vmn2r4	UTSW	3	64,296,855 (GRCm39)	nonsense	probably null
R0504:Vmn2r4	UTSW	3	64,296,784 (GRCm39)	missense	probably damaging	1.00
R1546:Vmn2r4	UTSW	3	64,314,309 (GRCm39)	missense	probably damaging	1.00
R1562:Vmn2r4	UTSW	3	64,296,865 (GRCm39)	missense	probably damaging	0.98
R1863:Vmn2r4	UTSW	3	64,314,410 (GRCm39)	missense	probably benign	0.33
R1873:Vmn2r4	UTSW	3	64,298,479 (GRCm39)	missense	possibly damaging	0.93
R1939:Vmn2r4	UTSW	3	64,305,976 (GRCm39)	missense	probably benign	0.00
R2103:Vmn2r4	UTSW	3	64,322,704 (GRCm39)	missense	possibly damaging	0.48
R3083:Vmn2r4	UTSW	3	64,296,788 (GRCm39)	missense	probably damaging	1.00
R3687:Vmn2r4	UTSW	3	64,296,896 (GRCm39)	missense	possibly damaging	0.93
R3707:Vmn2r4	UTSW	3	64,296,895 (GRCm39)	missense	probably damaging	0.99
R3963:Vmn2r4	UTSW	3	64,322,572 (GRCm39)	missense	probably damaging	0.99
R4428:Vmn2r4	UTSW	3	64,322,590 (GRCm39)	missense	probably damaging	1.00
R4710:Vmn2r4	UTSW	3	64,317,201 (GRCm39)	critical splice donor site	probably null
R4737:Vmn2r4	UTSW	3	64,317,384 (GRCm39)	missense	probably damaging	1.00
R4767:Vmn2r4	UTSW	3	64,298,397 (GRCm39)	missense	probably damaging	0.99
R4776:Vmn2r4	UTSW	3	64,296,082 (GRCm39)	missense	probably damaging	0.96
R4834:Vmn2r4	UTSW	3	64,317,484 (GRCm39)	missense	probably benign	0.40
R4893:Vmn2r4	UTSW	3	64,313,676 (GRCm39)	missense	probably damaging	0.96
R4908:Vmn2r4	UTSW	3	64,296,476 (GRCm39)	missense	possibly damaging	0.59
R5049:Vmn2r4	UTSW	3	64,306,019 (GRCm39)	splice site	probably null
R5092:Vmn2r4	UTSW	3	64,298,373 (GRCm39)	missense	probably benign	0.01
R5234:Vmn2r4	UTSW	3	64,305,878 (GRCm39)	missense	possibly damaging	0.88
R5240:Vmn2r4	UTSW	3	64,314,358 (GRCm39)	missense	possibly damaging	0.53
R5704:Vmn2r4	UTSW	3	64,317,370 (GRCm39)	missense	probably benign	0.03
R5907:Vmn2r4	UTSW	3	64,298,487 (GRCm39)	missense	probably damaging	0.99
R5924:Vmn2r4	UTSW	3	64,296,685 (GRCm39)	missense	probably damaging	1.00
R6145:Vmn2r4	UTSW	3	64,314,364 (GRCm39)	missense	probably benign	0.00
R6191:Vmn2r4	UTSW	3	64,322,702 (GRCm39)	missense	probably benign	0.34
R6192:Vmn2r4	UTSW	3	64,322,699 (GRCm39)	missense	probably benign	0.00
R6207:Vmn2r4	UTSW	3	64,313,926 (GRCm39)	missense	probably damaging	1.00
R6457:Vmn2r4	UTSW	3	64,317,378 (GRCm39)	missense	probably damaging	1.00
R6533:Vmn2r4	UTSW	3	64,322,519 (GRCm39)	missense	probably benign
R6545:Vmn2r4	UTSW	3	64,313,777 (GRCm39)	missense	possibly damaging	0.50
R6594:Vmn2r4	UTSW	3	64,296,731 (GRCm39)	missense	probably damaging	1.00
R7049:Vmn2r4	UTSW	3	64,296,550 (GRCm39)	missense	probably benign	0.14
R7150:Vmn2r4	UTSW	3	64,305,898 (GRCm39)	missense	probably benign	0.01
R7187:Vmn2r4	UTSW	3	64,322,681 (GRCm39)	missense	probably benign	0.00
R7363:Vmn2r4	UTSW	3	64,314,432 (GRCm39)	missense	probably damaging	1.00
R7477:Vmn2r4	UTSW	3	64,305,850 (GRCm39)	missense	probably benign	0.01
R7675:Vmn2r4	UTSW	3	64,322,657 (GRCm39)	missense	probably benign	0.01
R7858:Vmn2r4	UTSW	3	64,317,226 (GRCm39)	missense	probably benign	0.00
R7888:Vmn2r4	UTSW	3	64,313,943 (GRCm39)	missense	probably damaging	0.99
R8678:Vmn2r4	UTSW	3	64,314,391 (GRCm39)	missense	probably benign
R8743:Vmn2r4	UTSW	3	64,317,247 (GRCm39)	missense	possibly damaging	0.95
R8841:Vmn2r4	UTSW	3	64,314,058 (GRCm39)	missense	probably damaging	0.97
R9671:Vmn2r4	UTSW	3	64,317,271 (GRCm39)	missense	probably benign	0.00
R9778:Vmn2r4	UTSW	3	64,322,497 (GRCm39)	missense	probably benign	0.15
X0019:Vmn2r4	UTSW	3	64,314,057 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAGAGTCACTTGTTGGCATGG -3'
(R):5'- AAGTGTTGCTCAGCTCCTATTC -3'

Sequencing Primer
(F):5'- GTTGGCATGGTCCTATAATGAACAG -3'
(R):5'- AATTGCATGTGACTGTGACATCTG -3'

Posted On

2017-02-15