Mutagenetix > Incidental Mutation

Incidental Mutation 'R0561:Mslnl'

45901

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0561 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 25743203 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 192 (Q192*)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably null
Transcript: ENSMUST00000047098
AA Change: Q192*

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: Q192*

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.9%
20x: 94.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adck1	A	G	12: 88,368,434	D30G	possibly damaging	Het
Apc	A	T	18: 34,313,303	H1050L	possibly damaging	Het
Armc2	A	G	10: 41,993,192	V166A	probably benign	Het
Atp6v1b1	A	T	6: 83,753,811	I173F	probably damaging	Het
Bpifb4	A	G	2: 153,944,822	D298G	probably damaging	Het
C4b	T	A	17: 34,734,417	S1031C	probably damaging	Het
Calcr	A	G	6: 3,692,630	I408T	probably damaging	Het
Catsperg1	T	C	7: 29,182,312	N1009S	probably damaging	Het
Ces2a	T	C	8: 104,737,533	S266P	probably benign	Het
Chrna1	A	G	2: 73,566,252	V433A	possibly damaging	Het
Ctnnb1	G	A	9: 120,951,722	V291M	probably damaging	Het
Dcbld1	T	C	10: 52,261,936	Y99H	probably benign	Het
Ddx60	A	T	8: 62,017,794	H1440L	possibly damaging	Het
Dsg1c	A	T	18: 20,274,775	I393L	probably benign	Het
Eif5	G	T	12: 111,540,516	R128L	probably benign	Het
Ercc3	A	G	18: 32,245,539	D191G	possibly damaging	Het
Gp1ba	A	T	11: 70,639,590		probably benign	Het
Krt24	C	T	11: 99,284,613	E199K	probably damaging	Het
Lrif1	G	T	3: 106,732,165	A164S	probably damaging	Het
Map2	A	G	1: 66,425,497	D1682G	probably damaging	Het
Megf8	C	T	7: 25,328,832	P274S	probably benign	Het
Nfkb2	T	C	19: 46,309,862	V535A	possibly damaging	Het
Olfr1196	A	G	2: 88,700,570	I253T	possibly damaging	Het
Olfr1206	T	A	2: 88,864,680	V25E	possibly damaging	Het
Olfr1353	T	A	10: 78,969,895	L82*	probably null	Het
Olfr1494	A	T	19: 13,749,298	Y64F	probably damaging	Het
Olfr884	G	T	9: 38,047,827	V202L	probably benign	Het
Olfr936	C	T	9: 39,047,373	M15I	probably damaging	Het
Pag1	T	A	3: 9,699,421	Y224F	probably damaging	Het
Pbrm1	A	C	14: 31,035,991	I193L	probably benign	Het
Phrf1	T	C	7: 141,254,963	V17A	probably benign	Het
Plekhg2	T	G	7: 28,370,483	T42P	probably benign	Het
Pmp22	T	A	11: 63,134,424	W28R	probably damaging	Het
Ppp1r13b	A	T	12: 111,866,446	H82Q	probably damaging	Het
Rgs8	T	C	1: 153,665,922		probably null	Het
Rtl1	A	G	12: 109,593,929	V492A	probably damaging	Het
Slc22a27	A	G	19: 7,880,162		probably null	Het
Slx4ip	A	G	2: 137,066,170	E79G	probably null	Het
Syde1	C	T	10: 78,589,376	R267H	probably damaging	Het
Tas2r114	A	G	6: 131,689,795	I90T	probably benign	Het
Tjp3	C	T	10: 81,273,840	G843D	probably benign	Het
Tln1	A	T	4: 43,550,304	M453K	possibly damaging	Het
Ttc39a	A	T	4: 109,440,602	Y408F	probably damaging	Het
Usp39	T	G	6: 72,336,385	Q274P	probably damaging	Het
Uvrag	C	T	7: 98,888,561	V476I	probably damaging	Het
Vcan	T	A	13: 89,712,253	T332S	probably damaging	Het
Vcan	T	A	13: 89,731,464	H22L	possibly damaging	Het
Wls	A	G	3: 159,873,068	D89G	probably benign	Het
Zfhx2	A	T	14: 55,065,889	V1546E	probably benign	Het
Zfp457	T	A	13: 67,294,070	H147L	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- AACTTGCCCAACCAGAGAGTTGCC -3'
(R):5'- CTGTCCTGGGTTCAAACCTCAAGC -3'

Sequencing Primer
(F):5'- TTCCTCAGATGGGGGTAAGATG -3'
(R):5'- GCATCTTATCTGATGCCCTCTGG -3'

Posted On

2013-06-11