Mutagenetix > Incidental Mutation

Incidental Mutation 'R5942:Gpr156'

460374

Institutional Source

Beutler Lab

Gene Symbol

Gpr156

Ensembl Gene

ENSMUSG00000046961

Gene Name

G protein-coupled receptor 156

Synonyms

Gababl

MMRRC Submission

044134-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5942 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

37736858-37827892 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 37825264 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 494 (P494S)

Ref Sequence

ENSEMBL: ENSMUSP00000055958 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061274]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000061274
AA Change: P494S

PolyPhen 2 Score 0.041 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000055958
Gene: ENSMUSG00000046961
AA Change: P494S

Domain	Start	End	E-Value	Type
low complexity region	38	50	N/A	INTRINSIC
Pfam:7tm_3	61	313	2.6e-37	PFAM
coiled coil region	353	390	N/A	INTRINSIC
low complexity region	584	593	N/A	INTRINSIC
low complexity region	635	648	N/A	INTRINSIC
low complexity region	681	716	N/A	INTRINSIC
low complexity region	729	739	N/A	INTRINSIC
low complexity region	753	766	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.3%
10x: 96.7%
20x: 89.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] G protein-coupled receptors (GPCRs) are a large superfamily of cell surface receptors characterized by 7 helical transmembrane domains, together with N-terminal extracellular and C-terminal intracellular domains.[supplied by OMIM, Mar 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	C	T	17: 46,623,333 (GRCm39)	V860M	probably benign	Het
Accs	A	T	2: 93,666,392 (GRCm39)	L432M	probably damaging	Het
Actrt3	T	A	3: 30,652,813 (GRCm39)	N94Y	possibly damaging	Het
Adamts18	T	C	8: 114,504,380 (GRCm39)	Q80R	probably benign	Het
Ccr8	G	T	9: 119,923,772 (GRCm39)	V296F	probably damaging	Het
Cep170	T	A	1: 176,583,985 (GRCm39)	E798V	probably damaging	Het
Cttnbp2	T	A	6: 18,448,439 (GRCm39)	E73D	probably damaging	Het
Cyp2a4	A	T	7: 26,010,129 (GRCm39)		probably null	Het
Dync2h1	A	T	9: 7,117,466 (GRCm39)	Y41*	probably null	Het
Enc1	G	A	13: 97,382,887 (GRCm39)	D466N	probably benign	Het
Enpp1	C	A	10: 24,551,966 (GRCm39)	E138*	probably null	Het
Entrep1	A	G	19: 23,963,834 (GRCm39)	V245A	probably damaging	Het
Ezh2	T	C	6: 47,554,516 (GRCm39)	R27G	possibly damaging	Het
Fut10	A	G	8: 31,691,485 (GRCm39)	N110S	possibly damaging	Het
Glt1d1	A	T	5: 127,721,534 (GRCm39)		probably null	Het
Gm14393	G	A	2: 174,903,689 (GRCm39)	Q73*	probably null	Het
Has2	T	A	15: 56,531,192 (GRCm39)	K508*	probably null	Het
Hc	A	T	2: 34,918,137 (GRCm39)	C715*	probably null	Het
Hook2	C	T	8: 85,721,409 (GRCm39)		probably null	Het
Klhdc7b	A	C	15: 89,271,634 (GRCm39)	I839L	probably benign	Het
Kndc1	T	C	7: 139,516,792 (GRCm39)	L1584P	probably damaging	Het
Lamp1	T	C	8: 13,223,941 (GRCm39)	F358L	probably damaging	Het
Man2a1	A	G	17: 64,932,375 (GRCm39)	K154R	probably benign	Het
Mga	A	G	2: 119,777,440 (GRCm39)	I1871V	probably benign	Het
Mgmt	T	A	7: 136,723,219 (GRCm39)	D96E	probably benign	Het
Morc2a	C	A	11: 3,629,936 (GRCm39)	T424K	probably damaging	Het
Myo1g	A	G	11: 6,464,888 (GRCm39)	L462P	probably damaging	Het
Ncaph	C	A	2: 126,958,608 (GRCm39)		probably null	Het
Nlrc3	A	T	16: 3,767,293 (GRCm39)	D969E	probably damaging	Het
Nt5c3	A	T	6: 56,874,839 (GRCm39)		probably null	Het
Or4c12b	G	T	2: 89,646,684 (GRCm39)	E5*	probably null	Het
Or5ac15	A	T	16: 58,940,039 (GRCm39)	Y131*	probably null	Het
Or5d41	A	C	2: 88,054,916 (GRCm39)	I153M	probably benign	Het
Or8h8	T	C	2: 86,753,750 (GRCm39)	N42S	probably damaging	Het
Parp14	C	A	16: 35,659,737 (GRCm39)	M1628I	probably damaging	Het
Parp8	G	T	13: 117,005,969 (GRCm39)	P693Q	probably benign	Het
Parp9	A	G	16: 35,792,259 (GRCm39)	D485G	possibly damaging	Het
Pcdha1	T	A	18: 37,063,444 (GRCm39)	V36D	probably damaging	Het
Pcdhb5	C	T	18: 37,453,838 (GRCm39)	Q73*	probably null	Het
Pecam1	G	T	11: 106,552,809 (GRCm39)		probably benign	Het
Pex1	A	G	5: 3,660,277 (GRCm39)	I527V	probably benign	Het
Phf11	G	A	14: 59,497,593 (GRCm39)	P13S	probably benign	Het
Ppp4r4	T	A	12: 103,553,706 (GRCm39)	V388D	possibly damaging	Het
Psmc4	C	T	7: 27,746,480 (GRCm39)	V202I	probably damaging	Het
Ptx3	T	G	3: 66,127,484 (GRCm39)	M1R	probably null	Het
Rimbp3	A	G	16: 17,029,752 (GRCm39)	T1059A	probably benign	Het
Rmdn3	G	A	2: 118,978,058 (GRCm39)	A181V	probably damaging	Het
Rnase10	A	T	14: 51,246,735 (GRCm39)	M38L	probably benign	Het
Sec16b	T	A	1: 157,358,920 (GRCm39)	Y118N	probably damaging	Het
Sigmar1	T	C	4: 41,741,159 (GRCm39)	T32A	probably benign	Het
Srcap	A	G	7: 127,137,180 (GRCm39)	D954G	probably damaging	Het
Tfrc	A	G	16: 32,445,533 (GRCm39)	N618S	possibly damaging	Het
Tnrc6a	A	G	7: 122,785,888 (GRCm39)	D1028G	probably damaging	Het
Tns3	A	T	11: 8,385,860 (GRCm39)	D1379E	probably damaging	Het
Ttn	A	T	2: 76,580,505 (GRCm39)	C23463S	possibly damaging	Het
Ufl1	T	C	4: 25,250,619 (GRCm39)	T745A	probably benign	Het
Vmn1r231	T	A	17: 21,110,417 (GRCm39)	Y166F	possibly damaging	Het
Wbp1l	C	A	19: 46,642,869 (GRCm39)	T290K	probably damaging	Het
Wdr25	A	G	12: 108,864,392 (GRCm39)	N179S	probably benign	Het
Wdr62	G	A	7: 29,942,504 (GRCm39)	Q1035*	probably null	Het
Yif1a	C	T	19: 5,141,669 (GRCm39)	R196C	probably damaging	Het
Zfp352	A	T	4: 90,113,307 (GRCm39)	K482N	probably damaging	Het
Zfp647	G	A	15: 76,796,285 (GRCm39)	P125L	probably damaging	Het

Other mutations in Gpr156

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00943:Gpr156	APN	16	37,808,938 (GRCm39)	missense	probably damaging	1.00
IGL01615:Gpr156	APN	16	37,808,953 (GRCm39)	missense	probably damaging	1.00
IGL01976:Gpr156	APN	16	37,799,395 (GRCm39)	missense	probably damaging	0.97
IGL02217:Gpr156	APN	16	37,825,673 (GRCm39)	missense	probably benign	0.19
IGL02515:Gpr156	APN	16	37,826,041 (GRCm39)	missense	probably damaging	0.97
IGL02596:Gpr156	APN	16	37,799,086 (GRCm39)	missense	probably benign	0.00
IGL03068:Gpr156	APN	16	37,812,491 (GRCm39)	missense	probably damaging	0.99
R0690:Gpr156	UTSW	16	37,812,503 (GRCm39)	missense	probably damaging	1.00
R1034:Gpr156	UTSW	16	37,825,088 (GRCm39)	missense	probably benign
R1133:Gpr156	UTSW	16	37,825,683 (GRCm39)	missense	probably benign	0.10
R1317:Gpr156	UTSW	16	37,807,929 (GRCm39)	missense	probably damaging	1.00
R1437:Gpr156	UTSW	16	37,808,904 (GRCm39)	missense	probably damaging	0.99
R1484:Gpr156	UTSW	16	37,812,558 (GRCm39)	missense	probably damaging	0.99
R1759:Gpr156	UTSW	16	37,768,583 (GRCm39)	missense	probably damaging	0.96
R1761:Gpr156	UTSW	16	37,807,929 (GRCm39)	missense	probably damaging	1.00
R1998:Gpr156	UTSW	16	37,818,270 (GRCm39)	missense	possibly damaging	0.57
R2067:Gpr156	UTSW	16	37,799,113 (GRCm39)	missense	probably benign	0.02
R2111:Gpr156	UTSW	16	37,799,113 (GRCm39)	missense	probably benign	0.02
R2509:Gpr156	UTSW	16	37,768,149 (GRCm39)	missense	probably benign	0.04
R2872:Gpr156	UTSW	16	37,812,585 (GRCm39)	missense	probably damaging	0.99
R2872:Gpr156	UTSW	16	37,812,585 (GRCm39)	missense	probably damaging	0.99
R3839:Gpr156	UTSW	16	37,808,962 (GRCm39)	missense	probably damaging	0.99
R4492:Gpr156	UTSW	16	37,812,468 (GRCm39)	missense	probably damaging	0.99
R4988:Gpr156	UTSW	16	37,768,577 (GRCm39)	missense	possibly damaging	0.71
R5329:Gpr156	UTSW	16	37,825,810 (GRCm39)	missense	probably benign	0.00
R5361:Gpr156	UTSW	16	37,826,087 (GRCm39)	missense	probably damaging	0.99
R5386:Gpr156	UTSW	16	37,768,671 (GRCm39)	missense	possibly damaging	0.93
R5531:Gpr156	UTSW	16	37,825,619 (GRCm39)	missense	probably benign	0.01
R5886:Gpr156	UTSW	16	37,799,375 (GRCm39)	missense	probably damaging	1.00
R6345:Gpr156	UTSW	16	37,807,881 (GRCm39)	missense	probably damaging	1.00
R7247:Gpr156	UTSW	16	37,768,103 (GRCm39)	missense	probably damaging	1.00
R7353:Gpr156	UTSW	16	37,812,523 (GRCm39)	missense	probably damaging	1.00
R7954:Gpr156	UTSW	16	37,807,920 (GRCm39)	missense	probably damaging	0.97
R8316:Gpr156	UTSW	16	37,818,336 (GRCm39)	missense	probably null	0.00
R8333:Gpr156	UTSW	16	37,812,416 (GRCm39)	missense	probably damaging	1.00
R8507:Gpr156	UTSW	16	37,768,598 (GRCm39)	missense	probably benign
R8770:Gpr156	UTSW	16	37,824,974 (GRCm39)	missense	possibly damaging	0.94
R9237:Gpr156	UTSW	16	37,825,648 (GRCm39)	nonsense	probably null
R9491:Gpr156	UTSW	16	37,825,704 (GRCm39)	missense	probably benign	0.03
R9767:Gpr156	UTSW	16	37,818,297 (GRCm39)	missense	probably damaging	1.00
Z1177:Gpr156	UTSW	16	37,825,225 (GRCm39)	missense	probably benign	0.22

Predicted Primers

PCR Primer
(F):5'- TCGGAATGCCCTGCTGTTTC -3'
(R):5'- CTGAAACCCAGTTTAGATGGGG -3'

Sequencing Primer
(F):5'- GGACTCCCTACACGTTCCAG -3'
(R):5'- GGCTACGGGAAGTGGTACTC -3'

Posted On

2017-02-28