Mutagenetix > Incidental Mutation

Incidental Mutation 'R5924:Or4k15'

461747

Institutional Source

Beutler Lab

Gene Symbol

Or4k15

Ensembl Gene

ENSMUSG00000059488

Gene Name

olfactory receptor family 4 subfamily K member 15

Synonyms

Olfr727, GA_x6K02T2PMLR-5817082-5818056, MOR246-2

MMRRC Submission

044119-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.140) question?

Stock #

R5924 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

50364010-50365044 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 50364139 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 35 (Y35C)

Ref Sequence

ENSEMBL: ENSMUSP00000149886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079142] [ENSMUST00000215317]

AlphaFold

A2RTN7

Predicted Effect

probably damaging
Transcript: ENSMUST00000079142
AA Change: Y35C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078145
Gene: ENSMUSG00000059488
AA Change: Y35C

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	8.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	36	290	1.5e-7	PFAM
Pfam:7tm_1	41	287	1.1e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205947

Predicted Effect

probably damaging
Transcript: ENSMUST00000215317
AA Change: Y35C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 97.6%
20x: 92.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd3	T	C	18: 10,706,085 (GRCm39)	Y76C	probably damaging	Het
Agbl1	C	T	7: 76,058,982 (GRCm39)	T204I	probably benign	Het
Apc2	A	T	10: 80,147,984 (GRCm39)	I984F	probably damaging	Het
Art3	A	T	5: 92,560,091 (GRCm39)		probably benign	Het
B4galnt4	A	G	7: 140,650,742 (GRCm39)	M839V	probably damaging	Het
Bnip2	A	G	9: 69,904,444 (GRCm39)	D67G	probably benign	Het
Cdhr2	A	C	13: 54,874,496 (GRCm39)	D856A	probably benign	Het
Cep78	A	T	19: 15,938,430 (GRCm39)	L506Q	probably damaging	Het
Col6a1	A	G	10: 76,554,205 (GRCm39)		probably null	Het
Cyp3a44	A	T	5: 145,731,137 (GRCm39)	F221Y	possibly damaging	Het
Dcakd	C	A	11: 102,890,646 (GRCm39)	R47L	probably benign	Het
Ddr2	A	G	1: 169,822,197 (GRCm39)	V417A	probably benign	Het
Dnah5	A	T	15: 28,307,473 (GRCm39)	T1734S	probably benign	Het
Eefsec	A	T	6: 88,332,529 (GRCm39)	M227K	probably damaging	Het
Eif4g3	T	G	4: 137,929,237 (GRCm39)	N1628K	probably damaging	Het
Epha5	A	T	5: 84,381,533 (GRCm39)	Y439*	probably null	Het
Esrp1	G	T	4: 11,361,174 (GRCm39)	T324K	probably damaging	Het
Flnb	T	A	14: 7,890,765 (GRCm38)	M549K	probably benign	Het
Fndc1	T	A	17: 7,992,442 (GRCm39)	Q418L	unknown	Het
Ggnbp2	A	G	11: 84,749,363 (GRCm39)	S144P	possibly damaging	Het
Gk5	T	C	9: 96,032,563 (GRCm39)		probably null	Het
Gpr137	A	G	19: 6,916,729 (GRCm39)	L228P	probably damaging	Het
Gpt2	C	A	8: 86,219,633 (GRCm39)	S26R	probably damaging	Het
Hras	C	T	7: 140,772,374 (GRCm39)	E91K	possibly damaging	Het
Ighv1-36	G	A	12: 114,843,777 (GRCm39)	P28S	possibly damaging	Het
Kalrn	G	A	16: 34,064,203 (GRCm39)	T807M	probably damaging	Het
Lifr	A	G	15: 7,202,453 (GRCm39)	T365A	probably benign	Het
Lpin1	A	T	12: 16,594,658 (GRCm39)	S795T	possibly damaging	Het
Magi2	A	C	5: 20,816,067 (GRCm39)	M1128L	probably benign	Het
Magi3	A	T	3: 103,961,854 (GRCm39)		probably null	Het
Mier1	T	A	4: 103,016,899 (GRCm39)	L380*	probably null	Het
Mtmr14	A	G	6: 113,230,750 (GRCm39)	Y118C	probably damaging	Het
Myof	A	T	19: 37,971,421 (GRCm39)	M277K	probably damaging	Het
Nlrp6	T	C	7: 140,503,403 (GRCm39)	V473A	probably damaging	Het
Nsfl1c	T	A	2: 151,347,320 (GRCm39)	N164K	probably benign	Het
Olfm3	A	T	3: 114,916,187 (GRCm39)	Q353L	probably benign	Het
Opn5	A	T	17: 42,922,199 (GRCm39)	M1K	probably null	Het
Or2y3	A	G	17: 38,393,254 (GRCm39)	V205A	probably benign	Het
Or5d41	T	C	2: 88,054,891 (GRCm39)	I162V	probably benign	Het
Pax8	A	G	2: 24,311,634 (GRCm39)	S434P	probably damaging	Het
Pigo	G	A	4: 43,023,389 (GRCm39)	Q256*	probably null	Het
Pik3ap1	A	C	19: 41,284,895 (GRCm39)	F597V	probably damaging	Het
Pkd2	A	G	5: 104,646,424 (GRCm39)	K744E	probably damaging	Het
Prom1	T	C	5: 44,162,305 (GRCm39)	T729A	probably benign	Het
Rasal1	T	C	5: 120,813,582 (GRCm39)	L652P	probably damaging	Het
Sebox	T	C	11: 78,395,017 (GRCm39)		probably null	Het
Setd2	A	T	9: 110,403,112 (GRCm39)	I1918F	probably benign	Het
Slc24a2	A	T	4: 86,929,825 (GRCm39)		probably null	Het
Slc28a1	G	T	7: 80,765,360 (GRCm39)	G25V	probably benign	Het
Slc51a	A	G	16: 32,295,990 (GRCm39)	F259L	possibly damaging	Het
Slco2a1	T	A	9: 102,923,898 (GRCm39)	C37*	probably null	Het
Speer4f2	A	G	5: 17,581,622 (GRCm39)	D188G	probably damaging	Het
Stim2	A	G	5: 54,259,985 (GRCm39)	K156E	probably benign	Het
Strn4	T	C	7: 16,572,246 (GRCm39)	I653T	probably damaging	Het
Tacr3	G	T	3: 134,638,060 (GRCm39)	D406Y	possibly damaging	Het
Utp20	G	A	10: 88,651,784 (GRCm39)	R400C	probably benign	Het
V1rd19	C	T	7: 23,703,374 (GRCm39)	S280L	probably benign	Het
Vmn2r4	C	T	3: 64,296,685 (GRCm39)	C700Y	probably damaging	Het
Zup1	A	T	10: 33,803,543 (GRCm39)	C514S	probably damaging	Het

Other mutations in Or4k15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00906:Or4k15	APN	14	50,364,214 (GRCm39)	missense	probably damaging	1.00
IGL01306:Or4k15	APN	14	50,364,039 (GRCm39)	missense	probably benign	0.00
ANU23:Or4k15	UTSW	14	50,364,039 (GRCm39)	missense	probably benign	0.00
R0498:Or4k15	UTSW	14	50,364,750 (GRCm39)	missense	probably damaging	1.00
R0574:Or4k15	UTSW	14	50,364,139 (GRCm39)	missense	probably damaging	1.00
R1201:Or4k15	UTSW	14	50,364,813 (GRCm39)	missense	probably damaging	1.00
R2112:Or4k15	UTSW	14	50,364,080 (GRCm39)	missense	probably damaging	1.00
R2435:Or4k15	UTSW	14	50,364,211 (GRCm39)	missense	probably damaging	1.00
R4238:Or4k15	UTSW	14	50,364,889 (GRCm39)	missense	probably benign
R4611:Or4k15	UTSW	14	50,364,530 (GRCm39)	missense	probably benign	0.12
R4663:Or4k15	UTSW	14	50,364,939 (GRCm39)	missense	probably benign	0.00
R4672:Or4k15	UTSW	14	50,364,714 (GRCm39)	missense	probably benign	0.02
R5022:Or4k15	UTSW	14	50,364,469 (GRCm39)	missense	possibly damaging	0.78
R5062:Or4k15	UTSW	14	50,364,894 (GRCm39)	missense	probably damaging	1.00
R6702:Or4k15	UTSW	14	50,364,688 (GRCm39)	missense	probably damaging	1.00
R6703:Or4k15	UTSW	14	50,364,688 (GRCm39)	missense	probably damaging	1.00
R7497:Or4k15	UTSW	14	50,364,952 (GRCm39)	missense	probably benign	0.20
R7615:Or4k15	UTSW	14	50,364,446 (GRCm39)	missense	probably benign	0.07
R7798:Or4k15	UTSW	14	50,364,895 (GRCm39)	missense	probably damaging	1.00
R8413:Or4k15	UTSW	14	50,364,827 (GRCm39)	missense	probably benign	0.19
R8439:Or4k15	UTSW	14	50,364,604 (GRCm39)	missense	probably benign
R8841:Or4k15	UTSW	14	50,364,666 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACTAGTGGCAGGTTTCTGAAAAC -3'
(R):5'- CCAAGCAGGCTTCAAAAGATATTG -3'

Sequencing Primer
(F):5'- tcaggctttatataactagatcc -3'
(R):5'- GTTTTTCGTTCAACCAGAAAGTCAGC -3'

Posted On

2017-02-28