Mutagenetix > Incidental Mutation

Incidental Mutation 'R0571:Zyg11b'

46444

Institutional Source

Beutler Lab

Gene Symbol

Zyg11b

Ensembl Gene

ENSMUSG00000034636

Gene Name

zyg-ll family member B, cell cycle regulator

Synonyms

1110046I03Rik, 2810482G21Rik, D4Mgi23, LOC242610

MMRRC Submission

038762-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.725) question?

Stock #

R0571 (G1)

Quality Score

216

Status

Validated

Chromosome

Chromosomal Location

108086921-108158293 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 108117239 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 334 (Y334N)

Ref Sequence

ENSEMBL: ENSMUSP00000043844 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043616]

AlphaFold

Q3UFS0

Predicted Effect

probably damaging
Transcript: ENSMUST00000043616
AA Change: Y334N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000043844
Gene: ENSMUSG00000034636
AA Change: Y334N

Domain	Start	End	E-Value	Type
SCOP:d1jdha_	370	722	3e-16	SMART
Blast:ARM	480	526	1e-17	BLAST
Blast:ARM	528	570	3e-19	BLAST
Blast:ARM	638	679	3e-10	BLAST

Meta Mutation Damage Score

0.9168

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.9%
20x: 94.7%

Validation Efficiency

100% (71/71)

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd4	C	T	14: 54,500,706 (GRCm39)	T165I	possibly damaging	Het
Acsl5	A	G	19: 55,277,343 (GRCm39)		probably benign	Het
Actl6b	C	A	5: 137,565,046 (GRCm39)		probably benign	Het
Atg13	T	C	2: 91,509,063 (GRCm39)		probably benign	Het
Cabyr	A	G	18: 12,883,909 (GRCm39)	E132G	probably damaging	Het
Cadps2	C	T	6: 23,583,411 (GRCm39)	V389I	probably damaging	Het
Capn2	C	T	1: 182,298,325 (GRCm39)	V647I	probably benign	Het
Card10	G	T	15: 78,671,601 (GRCm39)	P621Q	possibly damaging	Het
Catsperb	C	G	12: 101,569,033 (GRCm39)	H902D	possibly damaging	Het
Cers3	A	G	7: 66,435,805 (GRCm39)	M255V	possibly damaging	Het
Cfh	T	C	1: 140,030,071 (GRCm39)		probably null	Het
Chd3	A	C	11: 69,252,495 (GRCm39)		probably null	Het
Chpf2	G	T	5: 24,795,425 (GRCm39)	R316L	probably damaging	Het
Clca3a1	T	A	3: 144,713,550 (GRCm39)	N694Y	probably damaging	Het
Cplane1	A	G	15: 8,289,277 (GRCm39)	D2909G	unknown	Het
Ctbp2	G	A	7: 132,616,534 (GRCm39)	L44F	probably damaging	Het
Cttnbp2	T	C	6: 18,381,102 (GRCm39)	M1365V	probably benign	Het
D130052B06Rik	G	A	11: 33,573,922 (GRCm39)	R173H	probably benign	Het
Dchs1	A	G	7: 105,421,203 (GRCm39)	F406L	probably damaging	Het
Ddx43	T	A	9: 78,321,145 (GRCm39)	N384K	possibly damaging	Het
Drd5	G	A	5: 38,477,270 (GRCm39)	V88M	probably damaging	Het
Eefsec	A	T	6: 88,274,881 (GRCm39)	F361Y	probably benign	Het
Epb41	T	C	4: 131,717,215 (GRCm39)	D313G	probably damaging	Het
Etl4	T	C	2: 20,748,580 (GRCm39)	M104T	probably damaging	Het
Fabp7	A	T	10: 57,661,637 (GRCm39)	T37S	probably benign	Het
Fam186b	T	C	15: 99,184,834 (GRCm39)	T30A	probably benign	Het
Fam83d	G	A	2: 158,627,611 (GRCm39)	W433*	probably null	Het
Fmnl2	A	T	2: 52,944,503 (GRCm39)	T161S	probably benign	Het
Ghsr	C	T	3: 27,426,165 (GRCm39)	R74C	probably damaging	Het
Gtf3c3	C	T	1: 54,456,937 (GRCm39)	A488T	probably damaging	Het
Gtpbp4	A	T	13: 9,040,722 (GRCm39)		probably benign	Het
Hamp2	A	G	7: 30,623,511 (GRCm39)	L17P	possibly damaging	Het
Heatr1	C	A	13: 12,445,121 (GRCm39)	S1581R	probably damaging	Het
Hpf1	T	C	8: 61,353,147 (GRCm39)	V176A	probably benign	Het
Hydin	T	A	8: 111,240,735 (GRCm39)		probably null	Het
Ighg2c	G	A	12: 113,252,382 (GRCm39)	Q57*	probably null	Het
Itgb4	A	G	11: 115,870,594 (GRCm39)	N141S	possibly damaging	Het
Kif13b	G	T	14: 64,988,977 (GRCm39)	R786L	probably damaging	Het
Lhx3	C	A	2: 26,091,136 (GRCm39)	W391L	probably damaging	Het
Map1s	T	A	8: 71,365,551 (GRCm39)	V152D	probably damaging	Het
Map4	T	C	9: 109,865,834 (GRCm39)	M608T	probably benign	Het
Mb21d2	G	A	16: 28,748,324 (GRCm39)	A31V	probably benign	Het
Mfn1	T	C	3: 32,615,621 (GRCm39)	I328T	probably damaging	Het
Mif-ps9	G	A	19: 56,743,675 (GRCm39)		noncoding transcript	Het
Myh4	A	T	11: 67,141,157 (GRCm39)	I740F	possibly damaging	Het
Neo1	A	G	9: 58,893,069 (GRCm39)	V191A	probably benign	Het
Nfatc4	T	C	14: 56,067,485 (GRCm39)	V565A	probably damaging	Het
Nrxn2	G	C	19: 6,523,563 (GRCm39)	E525D	probably damaging	Het
Or12k8	T	C	2: 36,975,346 (GRCm39)	H138R	probably benign	Het
Pcdhb12	A	T	18: 37,570,261 (GRCm39)	D469V	probably damaging	Het
Pcdhb6	G	T	18: 37,468,167 (GRCm39)	V363L	probably benign	Het
Pkd1l2	T	C	8: 117,808,957 (GRCm39)	T78A	probably benign	Het
Primpol	T	G	8: 47,034,674 (GRCm39)	D418A	probably damaging	Het
Rbm12b1	G	A	4: 12,146,248 (GRCm39)	S740N	probably benign	Het
Rpe65	T	C	3: 159,305,986 (GRCm39)	L15P	probably damaging	Het
Rxrb	CGCGGCGGCGGCGGCGGCGGC	CGCGGCGGCGGCGGCGGC	17: 34,251,106 (GRCm39)		probably benign	Het
Sectm1a	A	G	11: 120,959,928 (GRCm39)		probably benign	Het
Sft2d1	C	A	17: 8,545,782 (GRCm39)		probably benign	Het
Slc22a18	A	G	7: 143,045,598 (GRCm39)		probably benign	Het
Slu7	G	A	11: 43,332,405 (GRCm39)		probably null	Het
Smc4	T	C	3: 68,931,622 (GRCm39)	V572A	probably damaging	Het
Spire2	T	A	8: 124,080,855 (GRCm39)	I33N	probably damaging	Het
Tbck	T	A	3: 132,458,403 (GRCm39)	C678S	probably damaging	Het
Tnrc6b	T	C	15: 80,797,539 (GRCm39)	V1362A	probably damaging	Het
Ttn	T	C	2: 76,570,326 (GRCm39)	K25110E	possibly damaging	Het
Ugt2b35	A	G	5: 87,148,793 (GRCm39)	S15G	possibly damaging	Het
Upf3a	T	A	8: 13,842,184 (GRCm39)	I200K	probably damaging	Het
Vill	G	C	9: 118,899,701 (GRCm39)	G295A	possibly damaging	Het
Vmn1r191	A	T	13: 22,363,217 (GRCm39)	V179D	probably damaging	Het
Vmn2r74	T	C	7: 85,601,629 (GRCm39)	T670A	probably damaging	Het
Zfp169	T	C	13: 48,643,166 (GRCm39)	T654A	possibly damaging	Het
Zfp646	A	G	7: 127,481,138 (GRCm39)	E1105G	probably damaging	Het

Other mutations in Zyg11b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Zyg11b	APN	4	108,094,613 (GRCm39)	missense	probably damaging	1.00
IGL01143:Zyg11b	APN	4	108,102,191 (GRCm39)	missense	possibly damaging	0.69
IGL01627:Zyg11b	APN	4	108,107,985 (GRCm39)	missense	probably benign	0.19
IGL02517:Zyg11b	APN	4	108,123,515 (GRCm39)	missense	probably damaging	0.96
IGL03166:Zyg11b	APN	4	108,123,086 (GRCm39)	missense	probably benign	0.32
R0326:Zyg11b	UTSW	4	108,129,450 (GRCm39)	missense	possibly damaging	0.77
R0345:Zyg11b	UTSW	4	108,123,604 (GRCm39)	missense	probably damaging	1.00
R0396:Zyg11b	UTSW	4	108,112,505 (GRCm39)	missense	probably damaging	1.00
R0718:Zyg11b	UTSW	4	108,099,273 (GRCm39)	missense	possibly damaging	0.94
R1426:Zyg11b	UTSW	4	108,108,009 (GRCm39)	missense	probably damaging	1.00
R1495:Zyg11b	UTSW	4	108,123,410 (GRCm39)	missense	probably damaging	1.00
R1829:Zyg11b	UTSW	4	108,123,290 (GRCm39)	missense	possibly damaging	0.65
R1907:Zyg11b	UTSW	4	108,112,423 (GRCm39)	missense	probably damaging	1.00
R1916:Zyg11b	UTSW	4	108,129,480 (GRCm39)	missense	probably damaging	0.99
R1980:Zyg11b	UTSW	4	108,123,127 (GRCm39)	missense	probably damaging	0.99
R2070:Zyg11b	UTSW	4	108,108,016 (GRCm39)	missense	possibly damaging	0.73
R2495:Zyg11b	UTSW	4	108,101,921 (GRCm39)	critical splice donor site	probably null
R4717:Zyg11b	UTSW	4	108,099,069 (GRCm39)	missense	probably damaging	0.99
R5889:Zyg11b	UTSW	4	108,094,577 (GRCm39)	nonsense	probably null
R5957:Zyg11b	UTSW	4	108,102,210 (GRCm39)	missense	probably damaging	1.00
R6606:Zyg11b	UTSW	4	108,093,286 (GRCm39)	missense	probably benign	0.00
R7151:Zyg11b	UTSW	4	108,102,119 (GRCm39)	missense	possibly damaging	0.49
R7316:Zyg11b	UTSW	4	108,107,699 (GRCm39)	missense	possibly damaging	0.81
R7488:Zyg11b	UTSW	4	108,123,655 (GRCm39)	missense	possibly damaging	0.65
R8936:Zyg11b	UTSW	4	108,109,356 (GRCm39)	missense
R9291:Zyg11b	UTSW	4	108,108,014 (GRCm39)	missense	probably benign	0.37
R9642:Zyg11b	UTSW	4	108,117,185 (GRCm39)	missense	probably damaging	1.00
X0022:Zyg11b	UTSW	4	108,093,298 (GRCm39)	missense	probably benign
X0067:Zyg11b	UTSW	4	108,112,543 (GRCm39)	missense	probably benign
Z1177:Zyg11b	UTSW	4	108,112,561 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCACACAGAGCTGTATGCTGAG -3'
(R):5'- GTTAGAAAGTAGCCACTCCCGCTTG -3'

Sequencing Primer
(F):5'- tcccctggaactagaattgtg -3'
(R):5'- GTTTCACACCTTACCTGGTTATG -3'

Posted On

2013-06-11