Mutagenetix > Incidental Mutations

Incidental Mutation 'R5969:Gm2035'

470755

Institutional Source

Beutler Lab

Gene Symbol

Gm2035

Ensembl Gene

ENSMUSG00000096619

Gene Name

predicted pseudogene 2035

Synonyms

MMRRC Submission

044152-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.222) question?

Stock #

R5969 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

87919423-87919857 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 87919478 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 127 (D127G)

Ref Sequence

ENSEMBL: ENSMUSP00000137146 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000180053]

Predicted Effect

unknown
Transcript: ENSMUST00000180053
AA Change: D127G

SMART Domains

Protein: ENSMUSP00000137146
Gene: ENSMUSG00000096619
AA Change: D127G

Domain	Start	End	E-Value	Type
low complexity region	3	25	N/A	INTRINSIC
eIF1a	28	110	4.13e-45	SMART
low complexity region	125	144	N/A	INTRINSIC

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.2%

Validation Efficiency

99% (67/68)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aaas	A	C	15: 102,350,564	Y19D	probably damaging	Het
Abca13	T	A	11: 9,292,214	L1359*	probably null	Het
Ahi1	A	T	10: 20,984,393	D671V	probably damaging	Het
Ahnak	T	C	19: 9,016,585	S5078P	probably damaging	Het
Ankhd1	A	T	18: 36,600,834	T584S	probably damaging	Het
Apba2	T	A	7: 64,744,447	L568*	probably null	Het
Cenpn	C	A	8: 116,940,537	L300I	probably damaging	Het
Cmya5	A	T	13: 93,089,544	L3012Q	possibly damaging	Het
Cnnm1	T	C	19: 43,491,472	S819P	probably damaging	Het
Cpa6	A	T	1: 10,488,883	S87T	probably benign	Het
Crybg2	T	A	4: 134,075,692		probably null	Het
Csmd1	T	A	8: 16,071,353	T1777S	probably benign	Het
Csmd3	G	T	15: 47,947,990	P1235Q	probably damaging	Het
Cxcr4	T	A	1: 128,589,847	N24Y	probably benign	Het
D630003M21Rik	G	T	2: 158,217,708	H91N	probably damaging	Het
Ece1	T	C	4: 137,961,740		probably null	Het
Edc3	T	C	9: 57,713,428	S11P	probably damaging	Het
Exoc3	G	A	13: 74,172,186	Q719*	probably null	Het
Fam13a	A	G	6: 58,965,198	M203T	probably damaging	Het
Fam160b1	C	T	19: 57,384,123	R602*	probably null	Het
Fchsd1	C	T	18: 37,959,873		probably benign	Het
Fxyd2	T	A	9: 45,408,330	I30N	probably damaging	Het
Gapt	A	G	13: 110,353,946	V61A	probably benign	Het
Glb1l2	C	T	9: 26,780,742	A74T	probably damaging	Het
Gpr35	T	C	1: 92,983,220	V2A	probably damaging	Het
Gtf3c1	A	G	7: 125,645,676	S1729P	possibly damaging	Het
Heatr5a	T	C	12: 51,959,040	T51A	probably benign	Het
Kat6b	G	A	14: 21,670,792	M1737I	probably damaging	Het
Kif20a	G	A	18: 34,632,415	A822T	probably benign	Het
Klk10	A	G	7: 43,784,985	Y267C	probably damaging	Het
Lgmn	T	C	12: 102,405,827	Y98C	probably damaging	Het
Lyst	A	C	13: 13,687,813		probably null	Het
Man2a1	A	G	17: 64,625,380	K154R	probably benign	Het
Mfng	A	T	15: 78,764,382	V165D	possibly damaging	Het
Mto1	A	G	9: 78,452,905	E225G	probably damaging	Het
Notch3	C	A	17: 32,153,884	C571F	probably damaging	Het
Nup205	C	T	6: 35,177,578		probably benign	Het
Olfr65	T	A	7: 103,906,910	I157N	probably damaging	Het
P2ry14	T	A	3: 59,115,158	I303F	probably damaging	Het
Pcnx3	T	C	19: 5,685,535	D421G	probably damaging	Het
Pdlim4	T	C	11: 54,063,656	H75R	possibly damaging	Het
Phf21a	A	T	2: 92,221,611	H17L	probably damaging	Het
Ppid	T	A	3: 79,597,717	N122K	probably damaging	Het
Ppp4r3a	T	C	12: 101,043,579	I613V	probably benign	Het
Prcp	C	T	7: 92,917,766	P229S	probably benign	Het
Ralgds	T	C	2: 28,542,414	V85A	probably damaging	Het
Rgs22	G	A	15: 36,015,636	T1034I	probably benign	Het
Slc4a3	T	C	1: 75,549,979	V48A	probably damaging	Het
Snx16	A	T	3: 10,438,157	M10K	possibly damaging	Het
Svep1	G	A	4: 58,070,977	Q2270*	probably null	Het
Tmem185b	T	G	1: 119,527,463	I318S	probably benign	Het
Tnik	C	T	3: 28,620,948	R657C	probably damaging	Het
Top3b	T	C	16: 16,883,565		probably null	Het
Trim40	C	T	17: 36,882,427	R203H	probably benign	Het
Triobp	T	A	15: 78,967,540	N631K	probably benign	Het
Ubr3	T	G	2: 69,979,386	Y1233*	probably null	Het
Vgll3	A	G	16: 65,839,563	D200G	probably damaging	Het
Vmn2r24	G	A	6: 123,779,022	E18K	probably benign	Het
Zfp141	C	A	7: 42,489,488	R40L	probably damaging	Het

Other mutations in Gm2035

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02314:Gm2035	APN	12	87919607	missense	probably benign	0.00
IGL02347:Gm2035	APN	12	87919589	missense	probably damaging	1.00
IGL02639:Gm2035	APN	12	87919499	missense	probably benign	0.04
R4230:Gm2035	UTSW	12	87919797	missense	unknown
R6159:Gm2035	UTSW	12	87919751	missense	probably damaging	1.00
R6359:Gm2035	UTSW	12	87919505	missense	probably benign	0.09
R7181:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7183:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7184:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7653:Gm2035	UTSW	12	87919478	missense	unknown
R8046:Gm2035	UTSW	12	87919565	missense	probably benign	0.02
R8353:Gm2035	UTSW	12	87919553	missense	probably benign
R8453:Gm2035	UTSW	12	87919553	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCATTGACAAGACACACTCATG -3'
(R):5'- CCAAAATGCTGGGATGTGGAC -3'

Sequencing Primer
(F):5'- CACACTCATGCCAAATGAAGTTTTAC -3'
(R):5'- TGTAAGGAGGCTGTGCAAC -3'

Posted On

2017-03-31