Incidental Mutation 'R5970:Cry2'
ID 470787
Institutional Source Beutler Lab
Gene Symbol Cry2
Ensembl Gene ENSMUSG00000068742
Gene Name cryptochrome circadian regulator 2
Synonyms D130054K12Rik
MMRRC Submission 044153-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.540) question?
Stock # R5970 (G1)
Quality Score 225
Status Validated
Chromosome 2
Chromosomal Location 92233991-92264388 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 92243312 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Proline at position 510 (S510P)
Ref Sequence ENSEMBL: ENSMUSP00000106909 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]
AlphaFold Q9R194
Predicted Effect probably benign
Transcript: ENSMUST00000090559
AA Change: S510P

PolyPhen 2 Score 0.079 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: S510P

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 187 2.4e-50 PFAM
Pfam:FAD_binding_7 231 504 4.4e-89 PFAM
low complexity region 562 573 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000111278
AA Change: S510P

PolyPhen 2 Score 0.079 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: S510P

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 189 3.6e-50 PFAM
Pfam:FAD_binding_7 230 506 1.4e-105 PFAM
low complexity region 562 573 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126002
Meta Mutation Damage Score 0.1826 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.4%
Validation Efficiency 98% (63/64)
MGI Phenotype FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aldh1l1 G A 6: 90,574,028 (GRCm39) probably benign Het
Ambn T C 5: 88,615,810 (GRCm39) V413A possibly damaging Het
Amotl1 T A 9: 14,507,824 (GRCm39) D41V probably damaging Het
Arhgef26 T C 3: 62,247,468 (GRCm39) V184A probably benign Het
Birc6 G T 17: 74,925,497 (GRCm39) G936V possibly damaging Het
Ccser1 T A 6: 61,288,226 (GRCm39) S130T possibly damaging Het
Cecr2 A G 6: 120,697,868 (GRCm39) I56V probably damaging Het
Cfap52 A G 11: 67,821,570 (GRCm39) I486T probably damaging Het
Col4a3 A G 1: 82,694,050 (GRCm39) I1557V possibly damaging Het
Col6a5 T A 9: 105,823,046 (GRCm39) I104F unknown Het
Csmd2 G C 4: 128,439,944 (GRCm39) A3133P probably benign Het
Cyld G T 8: 89,459,621 (GRCm39) A611S probably damaging Het
Dennd4c G A 4: 86,743,749 (GRCm39) G1197E probably damaging Het
Dnah10 T C 5: 124,885,793 (GRCm39) F2969L probably benign Het
Dnai1 A G 4: 41,625,281 (GRCm39) K415R probably benign Het
Dnmbp T A 19: 43,842,610 (GRCm39) T1253S probably benign Het
Dsp T C 13: 38,379,678 (GRCm39) L1542P possibly damaging Het
Duox1 T A 2: 122,170,682 (GRCm39) L1234Q probably damaging Het
Efr3b T A 12: 4,018,590 (GRCm39) R585S possibly damaging Het
Gpt A G 15: 76,583,552 (GRCm39) probably null Het
Heatr6 G A 11: 83,644,544 (GRCm39) probably benign Het
Kcns2 A G 15: 34,839,930 (GRCm39) D431G probably benign Het
Kdm3b A G 18: 34,962,342 (GRCm39) N1543D probably damaging Het
Man2a1 A G 17: 64,932,375 (GRCm39) K154R probably benign Het
Mical3 G A 6: 120,935,232 (GRCm39) Q893* probably null Het
Morc3 C T 16: 93,663,341 (GRCm39) H515Y possibly damaging Het
Mprip A T 11: 59,648,547 (GRCm39) R750S probably damaging Het
Mroh1 G A 15: 76,335,691 (GRCm39) V1436M probably benign Het
Muc5ac C T 7: 141,344,406 (GRCm39) R69* probably null Het
Muc5b A T 7: 141,410,449 (GRCm39) Y1274F unknown Het
Mybpc1 A G 10: 88,378,318 (GRCm39) L674P probably damaging Het
Mypn A G 10: 62,966,802 (GRCm39) V958A probably benign Het
Nipbl T C 15: 8,326,302 (GRCm39) T2436A probably benign Het
Or7g20 A G 9: 18,946,443 (GRCm39) D8G probably benign Het
Pcdhb5 T A 18: 37,454,826 (GRCm39) L402Q probably damaging Het
Pigp T A 16: 94,171,053 (GRCm39) probably null Het
Rp1 A G 1: 4,418,685 (GRCm39) L809P probably benign Het
Scn3a T A 2: 65,325,125 (GRCm39) probably benign Het
Sdf2 A T 11: 78,136,906 (GRCm39) M29L probably benign Het
Serpina3b T G 12: 104,100,350 (GRCm39) L311V possibly damaging Het
Snx31 A T 15: 36,523,634 (GRCm39) Y349* probably null Het
Spidr A C 16: 15,932,733 (GRCm39) C182W probably damaging Het
St13 A T 15: 81,261,999 (GRCm39) S146R probably damaging Het
St8sia4 A G 1: 95,581,307 (GRCm39) V145A probably damaging Het
Stradb T C 1: 59,019,175 (GRCm39) probably null Het
Tcp11l2 T A 10: 84,430,661 (GRCm39) probably benign Het
Tfdp2 C T 9: 96,199,627 (GRCm39) P74S unknown Het
Tmprss15 C T 16: 78,854,547 (GRCm39) R287H probably benign Het
Trav10d T C 14: 53,048,779 (GRCm39) Y57H probably damaging Het
Vmn2r104 A G 17: 20,249,733 (GRCm39) I846T probably benign Het
Ywhah T A 5: 33,184,292 (GRCm39) M165K possibly damaging Het
Zfp324 C A 7: 12,703,293 (GRCm39) P72T probably benign Het
Other mutations in Cry2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01995:Cry2 APN 2 92,254,977 (GRCm39) missense probably benign 0.15
IGL02167:Cry2 APN 2 92,264,166 (GRCm39) missense possibly damaging 0.93
IGL02183:Cry2 APN 2 92,243,384 (GRCm39) missense probably damaging 0.99
IGL02343:Cry2 APN 2 92,257,266 (GRCm39) missense possibly damaging 0.90
IGL02432:Cry2 APN 2 92,244,012 (GRCm39) missense probably damaging 0.99
IGL02725:Cry2 APN 2 92,243,605 (GRCm39) splice site probably benign
IGL02932:Cry2 APN 2 92,243,462 (GRCm39) nonsense probably null
IGL03122:Cry2 APN 2 92,243,640 (GRCm39) missense probably damaging 1.00
IGL03366:Cry2 APN 2 92,244,060 (GRCm39) missense probably damaging 1.00
R0679:Cry2 UTSW 2 92,244,060 (GRCm39) missense probably damaging 1.00
R1325:Cry2 UTSW 2 92,244,115 (GRCm39) missense probably damaging 1.00
R1862:Cry2 UTSW 2 92,254,911 (GRCm39) missense probably damaging 1.00
R1891:Cry2 UTSW 2 92,243,985 (GRCm39) missense possibly damaging 0.93
R2189:Cry2 UTSW 2 92,242,037 (GRCm39) missense possibly damaging 0.84
R4032:Cry2 UTSW 2 92,244,172 (GRCm39) missense probably benign 0.00
R4689:Cry2 UTSW 2 92,254,899 (GRCm39) missense probably benign 0.38
R5130:Cry2 UTSW 2 92,254,944 (GRCm39) missense probably benign 0.28
R5145:Cry2 UTSW 2 92,243,405 (GRCm39) missense probably benign
R6179:Cry2 UTSW 2 92,244,187 (GRCm39) missense probably damaging 0.98
R7102:Cry2 UTSW 2 92,243,438 (GRCm39) missense probably damaging 0.99
R7158:Cry2 UTSW 2 92,244,060 (GRCm39) missense probably damaging 1.00
R7213:Cry2 UTSW 2 92,244,004 (GRCm39) missense probably benign 0.00
R7257:Cry2 UTSW 2 92,243,326 (GRCm39) missense possibly damaging 0.67
R7378:Cry2 UTSW 2 92,244,009 (GRCm39) missense probably damaging 1.00
R7427:Cry2 UTSW 2 92,243,392 (GRCm39) missense possibly damaging 0.74
R7428:Cry2 UTSW 2 92,243,392 (GRCm39) missense possibly damaging 0.74
R7440:Cry2 UTSW 2 92,243,983 (GRCm39) missense probably damaging 1.00
R7531:Cry2 UTSW 2 92,243,350 (GRCm39) missense probably damaging 0.98
R8234:Cry2 UTSW 2 92,242,974 (GRCm39) missense probably benign
R8350:Cry2 UTSW 2 92,244,286 (GRCm39) missense probably benign 0.00
R8450:Cry2 UTSW 2 92,244,286 (GRCm39) missense probably benign 0.00
R8496:Cry2 UTSW 2 92,257,284 (GRCm39) missense probably damaging 1.00
R9172:Cry2 UTSW 2 92,243,993 (GRCm39) missense probably damaging 1.00
R9283:Cry2 UTSW 2 92,244,249 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TAAGAGGGCTGCAGATCATG -3'
(R):5'- ACTGAAAGGCTTCCCCTCTC -3'

Sequencing Primer
(F):5'- GGGCTGCAGATCATGAGAAAC -3'
(R):5'- CGATACATCTATGAGCCCTGGAATG -3'
Posted On 2017-03-31