Mutagenetix > Incidental Mutation

Incidental Mutation 'R5957:Adgrg7'

471250

Institutional Source

Beutler Lab

Gene Symbol

Adgrg7

Ensembl Gene

ENSMUSG00000022755

Gene Name

adhesion G protein-coupled receptor G7

Synonyms

Gpr128, 9130020O16Rik

MMRRC Submission

043246-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5957 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

56724609-56795855 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 56773427 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 142 (N142I)

Ref Sequence

ENSEMBL: ENSMUSP00000023437 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023437]

AlphaFold

Q8BM96

Predicted Effect

probably damaging
Transcript: ENSMUST00000023437
AA Change: N142I

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000023437
Gene: ENSMUSG00000022755
AA Change: N142I

Domain	Start	End	E-Value	Type
transmembrane domain	12	34	N/A	INTRINSIC
SCOP:d1edmb_	52	76	1e-3	SMART
GPS	376	424	6.16e-8	SMART
Pfam:7tm_2	428	712	4.5e-40	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced body weight gain and increased frequency of peristalsis and slow wave potential in the small intestine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,070,161	T1704A	probably benign	Het
Aldh18a1	A	T	19: 40,570,537	Y286*	probably null	Het
Armc4	T	C	18: 7,285,706	E219G	probably benign	Het
Arpp21	T	C	9: 112,185,686	T17A	probably benign	Het
Atp5s	T	C	12: 69,743,784	V185A	probably benign	Het
Bnip2	T	C	9: 69,999,238	I147T	probably damaging	Het
Ccr8	T	C	9: 120,093,827	Y3H	probably damaging	Het
Cenps	C	A	4: 149,130,201		probably benign	Het
Cul7	G	A	17: 46,657,757	G553S	probably damaging	Het
Cyp21a1	A	G	17: 34,803,176	I206T	probably benign	Het
Dennd4b	A	G	3: 90,270,965	D488G	probably damaging	Het
Dip2b	T	C	15: 100,209,694	L1195P	probably benign	Het
Dock5	T	A	14: 67,857,994	H77L	probably benign	Het
Fbxw13	C	T	9: 109,192,666		probably null	Het
Fmnl3	G	A	15: 99,325,910	R302W	probably damaging	Het
Gbf1	G	T	19: 46,246,221		probably null	Het
Gm12794	T	C	4: 101,941,701	F290L	probably benign	Het
Gm4846	T	C	1: 166,486,953	I374V	probably benign	Het
Gsk3b	C	A	16: 38,193,953	P258T	probably damaging	Het
Igsf5	T	C	16: 96,364,049	V8A	probably benign	Het
Il22	T	A	10: 118,205,166	L59Q	probably damaging	Het
Ildr1	T	C	16: 36,725,534	*517Q	probably null	Het
Iqca	T	A	1: 90,080,948	D450V	probably damaging	Het
Itga5	T	C	15: 103,351,429	D647G	probably benign	Het
Myh7	T	G	14: 54,989,078	N408T	probably damaging	Het
Mylk3	T	C	8: 85,328,637	M564V	probably damaging	Het
Nsd2	T	A	5: 33,855,603	M407K	probably damaging	Het
Oprd1	C	T	4: 132,144,163	V75I	probably benign	Het
Poli	G	A	18: 70,517,440	H310Y	probably benign	Het
Ptch1	C	T	13: 63,525,115	R755H	probably damaging	Het
Pygl	A	T	12: 70,199,720	M351K	probably damaging	Het
Serpinb9b	T	C	13: 33,039,848	L341P	possibly damaging	Het
Slc47a1	A	G	11: 61,344,342	V555A	probably benign	Het
Slc8a2	C	A	7: 16,145,284	T565K	possibly damaging	Het
Snx14	T	C	9: 88,403,274	I446V	possibly damaging	Het
Syde1	T	C	10: 78,590,117	Y72C	probably damaging	Het
Trim37	C	T	11: 87,145,551	R138C	probably damaging	Het
Tubgcp5	T	A	7: 55,814,962	S530R	probably benign	Het
Vps13c	T	C	9: 67,954,971	S2957P	probably damaging	Het
Wdr41	T	C	13: 94,997,187		probably null	Het
Zyg11b	T	C	4: 108,245,013	K504E	probably damaging	Het

Other mutations in Adgrg7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01783:Adgrg7	APN	16	56747919	critical splice donor site	probably null
IGL03122:Adgrg7	APN	16	56770362	splice site	probably benign
orchard	UTSW	16	56724979	missense	probably damaging	1.00
sevin	UTSW	16	56742406	missense	probably damaging	1.00
R0632:Adgrg7	UTSW	16	56742589	missense	possibly damaging	0.89
R0673:Adgrg7	UTSW	16	56773486	missense	possibly damaging	0.48
R1690:Adgrg7	UTSW	16	56795630	missense	probably damaging	0.99
R2009:Adgrg7	UTSW	16	56761873	missense	probably benign	0.08
R2017:Adgrg7	UTSW	16	56732806	missense	probably benign	0.02
R2132:Adgrg7	UTSW	16	56767918	missense	probably damaging	1.00
R2153:Adgrg7	UTSW	16	56752428	missense	possibly damaging	0.75
R2229:Adgrg7	UTSW	16	56752403	missense	probably benign
R2436:Adgrg7	UTSW	16	56761945	missense	possibly damaging	0.78
R2878:Adgrg7	UTSW	16	56750454	missense	probably benign	0.14
R2981:Adgrg7	UTSW	16	56750406	critical splice donor site	probably null
R4014:Adgrg7	UTSW	16	56742288	missense	probably damaging	1.00
R4023:Adgrg7	UTSW	16	56730298	missense	probably damaging	1.00
R4024:Adgrg7	UTSW	16	56730298	missense	probably damaging	1.00
R4026:Adgrg7	UTSW	16	56730298	missense	probably damaging	1.00
R4551:Adgrg7	UTSW	16	56748012	missense	probably damaging	1.00
R4834:Adgrg7	UTSW	16	56732869	missense	probably damaging	1.00
R5041:Adgrg7	UTSW	16	56730348	missense	probably benign	0.21
R5145:Adgrg7	UTSW	16	56742319	missense	probably benign	0.04
R5377:Adgrg7	UTSW	16	56730306	missense	possibly damaging	0.68
R5549:Adgrg7	UTSW	16	56750427	missense	probably damaging	1.00
R5915:Adgrg7	UTSW	16	56730385	splice site	probably null
R6146:Adgrg7	UTSW	16	56773466	missense	probably benign	0.21
R6198:Adgrg7	UTSW	16	56777193	missense	possibly damaging	0.64
R6233:Adgrg7	UTSW	16	56778642	missense	possibly damaging	0.52
R6337:Adgrg7	UTSW	16	56752425	missense	probably damaging	0.96
R6633:Adgrg7	UTSW	16	56730286	missense	probably benign	0.05
R6693:Adgrg7	UTSW	16	56770224	missense	probably damaging	0.97
R6812:Adgrg7	UTSW	16	56795798	start gained	probably benign
R6841:Adgrg7	UTSW	16	56750424	missense	probably damaging	1.00
R6868:Adgrg7	UTSW	16	56773476	missense	probably benign
R7076:Adgrg7	UTSW	16	56742406	missense	probably damaging	1.00
R7146:Adgrg7	UTSW	16	56730242	missense	probably damaging	1.00
R7232:Adgrg7	UTSW	16	56777152	splice site	probably null
R7266:Adgrg7	UTSW	16	56770311	missense	probably benign	0.00
R7376:Adgrg7	UTSW	16	56724979	missense	probably damaging	1.00
R7390:Adgrg7	UTSW	16	56732844	missense	probably damaging	0.98
R7401:Adgrg7	UTSW	16	56742418	missense	probably benign	0.43
R7496:Adgrg7	UTSW	16	56732857	missense	probably benign
R7540:Adgrg7	UTSW	16	56750429	missense	probably damaging	1.00
R8147:Adgrg7	UTSW	16	56742513	missense	probably damaging	1.00
R8354:Adgrg7	UTSW	16	56795682	start gained	probably benign
R8372:Adgrg7	UTSW	16	56795751	start gained	probably benign
R8393:Adgrg7	UTSW	16	56762114	missense	probably damaging	1.00
R8454:Adgrg7	UTSW	16	56795682	start gained	probably benign
R8723:Adgrg7	UTSW	16	56761919	missense	probably benign	0.00
R8891:Adgrg7	UTSW	16	56752399	missense	probably benign	0.03
R9017:Adgrg7	UTSW	16	56732848	missense	probably benign	0.01
R9570:Adgrg7	UTSW	16	56750450	missense	probably damaging	1.00
R9604:Adgrg7	UTSW	16	56777207	missense	probably damaging	0.99
R9628:Adgrg7	UTSW	16	56732830	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTTGAGCAGTCTACAAATATAAGGTC -3'
(R):5'- AATACATGCAGTGCCCAGC -3'

Sequencing Primer
(F):5'- ATTTGCTAAGCTACCATTTGCTG -3'
(R):5'- ATGCAGTGCCCAGCAGAGTC -3'

Posted On

2017-03-31