Mutagenetix > Incidental Mutation

Incidental Mutation 'R5974:Zfp882'

471620

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

044157-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R5974 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 71913155 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 53 (F53L)

Ref Sequence

ENSEMBL: ENSMUSP00000119978 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably damaging
Transcript: ENSMUST00000110002
AA Change: F13L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: F13L

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000118290

Predicted Effect

probably damaging
Transcript: ENSMUST00000125802
AA Change: F21L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857
AA Change: F21L

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000126607
AA Change: F53L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857
AA Change: F53L

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170898

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afg3l2	G	T	18: 67,421,259 (GRCm38)	L458M	probably damaging	Het
Anapc4	T	C	5: 52,845,400 (GRCm38)	L261P	probably damaging	Het
Aqp6	A	G	15: 99,601,436 (GRCm38)	Y10C	probably benign	Het
Ccnb1ip1	A	T	14: 50,792,205 (GRCm38)	N133K	probably benign	Het
Clip4	A	T	17: 71,831,247 (GRCm38)	H433L	probably damaging	Het
Cntnap5c	T	A	17: 57,876,485 (GRCm38)	M62K	probably benign	Het
Col12a1	A	G	9: 79,682,127 (GRCm38)	S1049P	probably damaging	Het
Col15a1	C	T	4: 47,258,683 (GRCm38)	T358I	probably benign	Het
Coro7	A	T	16: 4,631,889 (GRCm38)	D645E	possibly damaging	Het
Ctnnal1	C	A	4: 56,817,067 (GRCm38)	W585L	probably damaging	Het
Ctnnd1	C	A	2: 84,620,915 (GRCm38)	E114*	probably null	Het
Daam2	T	A	17: 49,464,473 (GRCm38)	S882C	probably damaging	Het
Des	T	A	1: 75,362,984 (GRCm38)	S329T	probably benign	Het
Dido1	T	C	2: 180,671,497 (GRCm38)	D994G	probably benign	Het
Dock3	A	G	9: 106,994,062 (GRCm38)	V547A	probably damaging	Het
Ebf4	G	A	2: 130,365,564 (GRCm38)	A643T	probably damaging	Het
Ect2	C	A	3: 27,144,963 (GRCm38)	E194*	probably null	Het
Epb41l2	A	G	10: 25,441,815 (GRCm38)	I77V	possibly damaging	Het
Fat3	A	T	9: 16,006,528 (GRCm38)		probably null	Het
Fbn2	T	G	18: 58,048,920 (GRCm38)	D1803A	probably damaging	Het
Fbxo10	T	C	4: 45,040,631 (GRCm38)	E858G	probably benign	Het
Fbxo24	C	T	5: 137,619,650 (GRCm38)	R284Q	probably benign	Het
Fsip2	T	A	2: 82,963,313 (GRCm38)	I425N	possibly damaging	Het
Fut9	A	C	4: 25,620,090 (GRCm38)	Y241*	probably null	Het
Galr2	A	G	11: 116,283,026 (GRCm38)	S161G	possibly damaging	Het
Gcc2	T	A	10: 58,258,243 (GRCm38)	L14I	probably damaging	Het
Gm11127	A	T	17: 36,056,785 (GRCm38)	D220E	probably benign	Het
Hdac11	G	A	6: 91,173,214 (GRCm38)	V332I	probably benign	Het
Hdac7	T	C	15: 97,802,072 (GRCm38)		probably null	Het
Kif16b	C	T	2: 142,857,381 (GRCm38)	G93D	probably damaging	Het
Krtap11-1	C	A	16: 89,570,768 (GRCm38)	C121F	possibly damaging	Het
Lacc1	T	C	14: 77,035,077 (GRCm38)	Q93R	probably damaging	Het
Lama1	T	C	17: 67,773,727 (GRCm38)	F1250S	probably benign	Het
Lmbrd2	A	G	15: 9,172,115 (GRCm38)	E332G	probably benign	Het
Lrp2	C	A	2: 69,459,548 (GRCm38)	C3649F	probably damaging	Het
Map1a	T	C	2: 121,304,376 (GRCm38)	V1653A	probably benign	Het
Mrpl1	T	C	5: 96,231,794 (GRCm38)		probably null	Het
Myo1b	A	G	1: 51,778,373 (GRCm38)	S577P	probably damaging	Het
Nedd4	T	A	9: 72,743,638 (GRCm38)		probably null	Het
Negr1	T	A	3: 157,069,286 (GRCm38)	V213E	probably damaging	Het
Nlk	T	G	11: 78,590,966 (GRCm38)	Q223P	probably benign	Het
Ntn5	A	G	7: 45,691,424 (GRCm38)	H162R	probably damaging	Het
Nupl2	T	C	5: 24,167,402 (GRCm38)	S63P	probably damaging	Het
Obscn	C	A	11: 59,076,547 (GRCm38)	D477Y	probably damaging	Het
Olfr101	A	T	17: 37,300,338 (GRCm38)	I28N	possibly damaging	Het
Olfr1037	T	C	2: 86,084,881 (GRCm38)	S299G	probably benign	Het
Olfr1434	T	C	19: 12,283,836 (GRCm38)	S263P	probably damaging	Het
Pabpn1	A	G	14: 54,897,160 (GRCm38)	T280A	probably damaging	Het
Per3	A	T	4: 151,042,737 (GRCm38)	V109E	possibly damaging	Het
Pira2	A	C	7: 3,841,577 (GRCm38)	V485G	probably benign	Het
Pknox2	A	G	9: 36,936,322 (GRCm38)	L133P	probably damaging	Het
Pros1	A	C	16: 62,900,667 (GRCm38)	N195T	probably damaging	Het
Rffl	T	C	11: 82,806,151 (GRCm38)	K289E	probably damaging	Het
Ripk1	T	A	13: 34,030,101 (GRCm38)	Y475*	probably null	Het
Ryr2	A	G	13: 11,714,511 (GRCm38)		probably null	Het
Sgk1	T	A	10: 21,996,249 (GRCm38)	N241K	probably damaging	Het
Skint1	T	A	4: 112,019,319 (GRCm38)	S146T	probably benign	Het
Sox30	G	T	11: 45,981,073 (GRCm38)	D252Y	probably damaging	Het
Syngap1	A	G	17: 26,963,038 (GRCm38)	Y1002C	probably damaging	Het
Tiam2	A	G	17: 3,414,809 (GRCm38)	D271G	possibly damaging	Het
Ticam1	T	C	17: 56,271,178 (GRCm38)	T306A	probably benign	Het
Tmem252	A	G	19: 24,674,268 (GRCm38)	E67G	probably benign	Het
Tnxb	T	G	17: 34,685,707 (GRCm38)	F1149V	probably damaging	Het
Ubr4	A	G	4: 139,421,078 (GRCm38)		probably null	Het
Unc50	A	G	1: 37,437,209 (GRCm38)	D150G	probably benign	Het
Ywhag	A	C	5: 135,911,629 (GRCm38)	L37R	probably damaging	Het
Zfp296	T	C	7: 19,577,937 (GRCm38)	L123P	probably benign	Het
Zfp418	G	T	7: 7,182,200 (GRCm38)	Q387H	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71,913,827 (GRCm38)	missense	probably benign
R0244:Zfp882	UTSW	8	71,913,523 (GRCm38)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71,914,615 (GRCm38)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71,914,337 (GRCm38)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71,914,686 (GRCm38)	nonsense	probably null
R1299:Zfp882	UTSW	8	71,913,473 (GRCm38)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71,913,609 (GRCm38)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71,914,389 (GRCm38)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71,914,654 (GRCm38)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71,914,360 (GRCm38)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71,913,459 (GRCm38)	critical splice acceptor site	probably null
R6052:Zfp882	UTSW	8	71,914,505 (GRCm38)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71,914,640 (GRCm38)	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	71,914,286 (GRCm38)	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71,914,673 (GRCm38)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71,913,249 (GRCm38)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71,914,229 (GRCm38)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71,913,141 (GRCm38)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71,914,118 (GRCm38)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	71,914,987 (GRCm38)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	71,914,071 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCTTTTGCCTTATAATGAAGTCGC -3'
(R):5'- TGGAGCTTGTACAAATCTTGATCAG -3'

Sequencing Primer
(F):5'- AGTCGCAGTCTCACATTAGAG -3'
(R):5'- TCCTGAAGGTTTCCTGCA -3'

Posted On

2017-03-31