Mutagenetix > Incidental Mutation

Incidental Mutation 'R5975:Zfp341'

471665

Institutional Source

Beutler Lab

Gene Symbol

Zfp341

Ensembl Gene

ENSMUSG00000059842

Gene Name

zinc finger protein 341

Synonyms

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5975 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

154613297-154646821 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 154630441 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 315 (C315S)

Ref Sequence

ENSEMBL: ENSMUSP00000105324 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081926] [ENSMUST00000109702] [ENSMUST00000126421]

AlphaFold

Q6PGC9

Predicted Effect

probably damaging
Transcript: ENSMUST00000081926
AA Change: C322S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000080596
Gene: ENSMUSG00000059842
AA Change: C322S

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	320	342	3.69e-4	SMART
ZnF_C2H2	348	370	1.04e-3	SMART
ZnF_C2H2	442	464	1.45e-2	SMART
ZnF_C2H2	470	494	2.17e-1	SMART
ZnF_C2H2	500	522	2.91e-2	SMART
ZnF_C2H2	537	561	1.23e0	SMART
ZnF_C2H2	563	585	3.58e-2	SMART
ZnF_C2H2	591	613	1.62e0	SMART
ZnF_C2H2	619	641	2.27e-4	SMART
ZnF_C2H2	647	674	7.29e0	SMART
ZnF_C2H2	680	702	5.14e-3	SMART
low complexity region	740	759	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000109702
AA Change: C315S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105324
Gene: ENSMUSG00000059842
AA Change: C315S

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	313	335	3.69e-4	SMART
ZnF_C2H2	341	363	1.04e-3	SMART
ZnF_C2H2	435	457	1.45e-2	SMART
ZnF_C2H2	463	487	2.17e-1	SMART
ZnF_C2H2	493	515	2.91e-2	SMART
ZnF_C2H2	530	554	1.23e0	SMART
ZnF_C2H2	556	578	3.58e-2	SMART
ZnF_C2H2	584	606	1.62e0	SMART
ZnF_C2H2	612	634	2.27e-4	SMART
ZnF_C2H2	640	667	7.29e0	SMART
ZnF_C2H2	673	695	5.14e-3	SMART
low complexity region	733	752	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000126421

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153208

Meta Mutation Damage Score

0.9440

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.3%

Validation Efficiency

98% (80/82)

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438A13Rik	C	T	3: 36,969,221	T2233I	possibly damaging	Het
9430038I01Rik	T	C	7: 137,387,292		probably benign	Het
Abhd14a	A	T	9: 106,443,951		probably null	Het
Actn2	T	C	13: 12,340,497	N2D	probably benign	Het
Adcy9	T	C	16: 4,311,567	E722G	probably damaging	Het
Alox12	C	A	11: 70,242,783	V572L	possibly damaging	Het
Ankrd11	T	C	8: 122,889,749	I2434V	possibly damaging	Het
Anks1	T	A	17: 27,991,447		probably null	Het
Bpifa3	T	A	2: 154,136,321	S148T	probably damaging	Het
Bptf	C	T	11: 107,035,864		probably benign	Het
Cabin1	A	T	10: 75,657,839	L1655H	probably damaging	Het
Ccdc13	G	T	9: 121,827,235	Q171K	probably benign	Het
Ccdc33	T	A	9: 58,117,478	Q155L	possibly damaging	Het
Cckbr	G	A	7: 105,470,619	G280E	probably benign	Het
Cdk11b	G	A	4: 155,648,240		probably benign	Het
Celsr1	G	T	15: 85,919,038		probably null	Het
Cenpj	A	T	14: 56,564,066	I150N	possibly damaging	Het
Cep135	C	A	5: 76,640,890	A1110E	possibly damaging	Het
Chit1	C	A	1: 134,146,626	H224N	probably damaging	Het
Cul5	T	C	9: 53,622,793	R680G	probably damaging	Het
Dhx58	C	A	11: 100,702,209	R224L	probably damaging	Het
Dlx1	C	A	2: 71,531,009	N122K	probably damaging	Het
Dnah5	A	C	15: 28,234,282	D279A	probably damaging	Het
Enpp3	A	G	10: 24,774,842	W799R	probably benign	Het
Ercc5	A	G	1: 44,173,406	T675A	probably benign	Het
Farsa	T	A	8: 84,864,432		probably null	Het
Fbxw15	A	T	9: 109,555,252	V397D	probably damaging	Het
Fcrl5	G	A	3: 87,442,103	V62I	probably benign	Het
Gart	A	G	16: 91,624,336	S815P	probably damaging	Het
Glrx5	A	G	12: 105,040,323	N111S	possibly damaging	Het
Gm10845	A	G	14: 79,863,174		noncoding transcript	Het
Gm11639	A	G	11: 104,687,549		probably benign	Het
Gm8882	A	G	6: 132,362,073	S61P	unknown	Het
Gm9925	T	A	18: 74,065,516		probably benign	Het
Gsdme	T	C	6: 50,227,359	N206S	probably benign	Het
Helz2	T	C	2: 181,231,050	S2459G	probably benign	Het
Hnrnpul1	A	G	7: 25,754,359	S93P	possibly damaging	Het
Ints2	A	T	11: 86,226,748	I716N	possibly damaging	Het
Lmnb2	G	A	10: 80,905,128	Q248*	probably null	Het
Map3k7cl	A	G	16: 87,570,321	I32V	probably benign	Het
Mfsd4b4	A	T	10: 39,892,470	I255N	probably benign	Het
Myh6	A	G	14: 54,950,508	I1163T	probably benign	Het
Nphs1	A	T	7: 30,466,115	T636S	possibly damaging	Het
Ntsr1	T	A	2: 180,500,788	L124Q	probably damaging	Het
Obscn	C	T	11: 59,122,619		probably null	Het
P4ha2	G	A	11: 54,126,412		probably null	Het
Pcdha9	T	A	18: 36,999,111	V411D	probably benign	Het
Pkhd1l1	A	T	15: 44,525,988	I1380F	probably damaging	Het
Plekha1	T	C	7: 130,892,253	V106A	probably benign	Het
Plekhm1	A	T	11: 103,376,691	V818E	possibly damaging	Het
Pprc1	T	G	19: 46,065,370		probably benign	Het
Prmt9	T	C	8: 77,561,018		probably benign	Het
Rab26	T	C	17: 24,530,399	N193D	possibly damaging	Het
Rnf111	A	G	9: 70,429,580	S942P	probably damaging	Het
Scgb2b18	T	C	7: 33,173,225	T52A	probably damaging	Het
Syt3	C	T	7: 44,392,763	Q349*	probably null	Het
Tas2r107	T	C	6: 131,659,780	N102S	probably benign	Het
Tas2r126	T	A	6: 42,435,000	Y156N	possibly damaging	Het
Tcaf2	A	T	6: 42,642,778	I105N	probably benign	Het
Tet1	A	C	10: 62,879,773	M81R	probably benign	Het
Togaram2	T	C	17: 71,729,205	Y897H	probably damaging	Het
Trerf1	A	T	17: 47,314,271		noncoding transcript	Het
Ttn	G	A	2: 76,761,235	T12703I	probably damaging	Het
Unc79	A	G	12: 103,125,626	K1735E	possibly damaging	Het
Usp54	G	A	14: 20,583,351	T372I	possibly damaging	Het
Wdr24	T	C	17: 25,827,128	S476P	probably benign	Het
Wdr78	G	A	4: 103,049,589	P676S	probably benign	Het
Zbtb1	T	C	12: 76,386,275	I345T	possibly damaging	Het
Zcrb1	T	A	15: 93,395,615	I29L	probably benign	Het
Zfp623	C	A	15: 75,948,163	R323S	probably benign	Het
Zfp831	T	A	2: 174,644,092	Y187N	possibly damaging	Het

Other mutations in Zfp341

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00499:Zfp341	APN	2	154634231	missense	probably damaging	1.00
IGL01352:Zfp341	APN	2	154628896	missense	probably benign	0.00
IGL01748:Zfp341	APN	2	154628927	missense	probably damaging	0.99
IGL02260:Zfp341	APN	2	154642049	missense	possibly damaging	0.89
IGL02329:Zfp341	APN	2	154632304	missense	possibly damaging	0.90
casanova_grimbacher	UTSW	2	154624881	missense	probably damaging	1.00
Specious	UTSW	2	154646134	missense	possibly damaging	0.63
R0079:Zfp341	UTSW	2	154624994	nonsense	probably null
R0570:Zfp341	UTSW	2	154646068	missense	probably benign	0.02
R0620:Zfp341	UTSW	2	154634273	missense	possibly damaging	0.94
R1018:Zfp341	UTSW	2	154646052	missense	probably damaging	1.00
R1611:Zfp341	UTSW	2	154645703	missense	probably damaging	1.00
R1733:Zfp341	UTSW	2	154641378	missense	probably benign	0.19
R1822:Zfp341	UTSW	2	154646134	missense	possibly damaging	0.63
R1956:Zfp341	UTSW	2	154638212	missense	probably benign	0.09
R2437:Zfp341	UTSW	2	154628801	missense	probably damaging	0.97
R3623:Zfp341	UTSW	2	154624881	missense	probably damaging	1.00
R4417:Zfp341	UTSW	2	154628987	missense	possibly damaging	0.94
R4806:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4807:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4863:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4955:Zfp341	UTSW	2	154638030	missense	probably damaging	0.98
R4962:Zfp341	UTSW	2	154626814	missense	possibly damaging	0.80
R5484:Zfp341	UTSW	2	154625001	missense	probably benign	0.00
R5864:Zfp341	UTSW	2	154643554	missense	possibly damaging	0.95
R5877:Zfp341	UTSW	2	154632289	missense	probably damaging	1.00
R5990:Zfp341	UTSW	2	154645659	missense	probably damaging	0.98
R6057:Zfp341	UTSW	2	154625034	missense	probably benign	0.01
R6882:Zfp341	UTSW	2	154638023	missense	probably damaging	1.00
R7686:Zfp341	UTSW	2	154624861	missense	probably damaging	0.96
R7701:Zfp341	UTSW	2	154634080	splice site	probably null
R7847:Zfp341	UTSW	2	154634194	missense	probably damaging	1.00
R8109:Zfp341	UTSW	2	154627900	missense	probably benign	0.04
R9313:Zfp341	UTSW	2	154627987	missense	probably damaging	1.00
R9484:Zfp341	UTSW	2	154643843	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCAAGCCTTGGACTTTCC -3'
(R):5'- ATCTTCCTGTCTCAGCGAGTATG -3'

Sequencing Primer
(F):5'- AAGCCTTGGACTTTCCCTCTTACAG -3'
(R):5'- CTGTCTCAGCGAGTATGCTCAG -3'

Posted On

2017-03-31