Mutagenetix > Incidental Mutation

Incidental Mutation 'R5963:Zfp84'

471873

Institutional Source

Beutler Lab

Gene Symbol

Zfp84

Ensembl Gene

ENSMUSG00000046185

Gene Name

zinc finger protein 84

Synonyms

KRAB18, C86188, Zfp69, 2210410P13Rik, 4633401C23Rik

MMRRC Submission

044148-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5963 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

29467977-29479246 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 29476378 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 357 (G357C)

Ref Sequence

ENSEMBL: ENSMUSP00000032802 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032802]

AlphaFold

Q9D654

Predicted Effect

probably damaging
Transcript: ENSMUST00000032802
AA Change: G357C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000032802
Gene: ENSMUSG00000046185
AA Change: G357C

Domain	Start	End	E-Value	Type
KRAB	14	74	9.09e-36	SMART
ZnF_C2H2	249	271	1.67e-2	SMART
ZnF_C2H2	277	299	1.43e-1	SMART
ZnF_C2H2	305	327	5.81e-2	SMART
ZnF_C2H2	333	355	1.95e-3	SMART
ZnF_C2H2	361	383	8.6e-5	SMART
ZnF_C2H2	389	411	2.32e-1	SMART
ZnF_C2H2	417	439	3.89e-3	SMART
ZnF_C2H2	445	467	1.69e-3	SMART
ZnF_C2H2	473	495	9.58e-3	SMART
ZnF_C2H2	501	523	1.38e-3	SMART
ZnF_C2H2	529	551	1.58e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158514

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.5%

Validation Efficiency

99% (68/69)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alg8	A	G	7: 97,029,037 (GRCm39)	K131E	probably benign	Het
Ank3	T	A	10: 69,823,056 (GRCm39)	L575*	probably null	Het
Aqr	T	C	2: 113,957,442 (GRCm39)	T819A	probably damaging	Het
Bbs2	A	T	8: 94,807,659 (GRCm39)	S407T	probably benign	Het
Bmper	T	A	9: 23,286,889 (GRCm39)	C272S	probably benign	Het
Btbd9	C	T	17: 30,553,192 (GRCm39)		probably null	Het
C5ar1	A	G	7: 15,982,747 (GRCm39)	V91A	possibly damaging	Het
Ccdc138	A	G	10: 58,411,579 (GRCm39)	H649R	possibly damaging	Het
Cfap43	A	T	19: 47,734,013 (GRCm39)	V1451E	probably benign	Het
Cfap46	T	A	7: 139,231,511 (GRCm39)	M901L	probably damaging	Het
Cherp	G	T	8: 73,215,379 (GRCm39)		probably benign	Het
D430041D05Rik	C	T	2: 104,078,630 (GRCm39)	V1229I	possibly damaging	Het
Fat4	A	C	3: 39,064,696 (GRCm39)	D4884A	probably damaging	Het
Fcrl5	T	C	3: 87,351,480 (GRCm39)	F243L	probably damaging	Het
Garem1	T	A	18: 21,262,487 (GRCm39)	I776F	probably benign	Het
Gpr87	T	A	3: 59,086,690 (GRCm39)	R272*	probably null	Het
Gsdmc	A	T	15: 63,651,965 (GRCm39)		probably null	Het
Hydin	T	A	8: 111,220,926 (GRCm39)	F1441I	possibly damaging	Het
Ints11	T	A	4: 155,957,369 (GRCm39)	C63*	probably null	Het
Kdm4b	A	T	17: 56,706,732 (GRCm39)	T908S	probably damaging	Het
Lipn	A	G	19: 34,058,700 (GRCm39)	D304G	probably damaging	Het
Man2a1	T	G	17: 64,982,117 (GRCm39)	N544K	probably benign	Het
Mapkapk5	G	T	5: 121,676,544 (GRCm39)	H66N	probably damaging	Het
Mog	G	A	17: 37,323,240 (GRCm39)	R233*	probably null	Het
Mrps24	A	T	11: 5,657,481 (GRCm39)		probably benign	Het
Nom1	T	C	5: 29,642,768 (GRCm39)	L423P	probably damaging	Het
Or51b6	T	A	7: 103,556,168 (GRCm39)	V174E	probably benign	Het
Pcdha1	T	A	18: 37,064,224 (GRCm39)	V296E	probably damaging	Het
Pdcd2	T	C	17: 15,746,656 (GRCm39)	K168E	possibly damaging	Het
Pdcd2	G	T	17: 15,746,657 (GRCm39)	H167Q	probably damaging	Het
Pla2g12b	T	A	10: 59,239,780 (GRCm39)	V63D	probably damaging	Het
Pnn	C	T	12: 59,114,617 (GRCm39)	R56*	probably null	Het
Primpol	T	A	8: 47,046,615 (GRCm39)	E227V	possibly damaging	Het
Rad54l	A	T	4: 115,967,584 (GRCm39)	W233R	probably damaging	Het
Rims2	A	T	15: 39,300,578 (GRCm39)	D103V	probably damaging	Het
Rttn	T	C	18: 89,091,819 (GRCm39)	S1511P	probably benign	Het
Septin5	T	C	16: 18,442,962 (GRCm39)		probably null	Het
Simc1	T	A	13: 54,673,632 (GRCm39)	I660K	possibly damaging	Het
Slc15a5	A	G	6: 138,056,691 (GRCm39)	L75P	probably damaging	Het
Slc9a2	A	G	1: 40,721,196 (GRCm39)	S55G	possibly damaging	Het
Slit3	C	T	11: 35,591,063 (GRCm39)	R1292C	probably damaging	Het
Slitrk3	A	G	3: 72,958,046 (GRCm39)	V242A	probably benign	Het
St14	A	T	9: 31,017,853 (GRCm39)		probably benign	Het
Tnnt2	T	C	1: 135,771,600 (GRCm39)		probably benign	Het
Traf5	G	A	1: 191,731,977 (GRCm39)	T288I	probably benign	Het
Trerf1	C	T	17: 47,625,263 (GRCm39)		noncoding transcript	Het
Vegfc	A	G	8: 54,634,319 (GRCm39)	N333D	probably benign	Het
Vmn1r65	T	A	7: 6,011,608 (GRCm39)	I209F	probably damaging	Het
Vps51	A	G	19: 6,118,320 (GRCm39)	L725P	probably damaging	Het
Vps8	T	C	16: 21,288,871 (GRCm39)	I408T	possibly damaging	Het
Wdr72	T	C	9: 74,052,310 (GRCm39)	Y114H	probably damaging	Het
Ybx2	A	G	11: 69,831,918 (GRCm39)	E164G	probably damaging	Het
Zcchc14	A	T	8: 122,355,362 (GRCm39)		probably benign	Het
Zfp383	A	G	7: 29,615,103 (GRCm39)	T453A	possibly damaging	Het
Zmynd11	G	T	13: 9,745,931 (GRCm39)		probably benign	Het

Other mutations in Zfp84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01768:Zfp84	APN	7	29,476,091 (GRCm39)	missense	probably benign	0.16
IGL03022:Zfp84	APN	7	29,474,759 (GRCm39)	splice site	probably benign
R0666:Zfp84	UTSW	7	29,476,276 (GRCm39)	missense	probably damaging	1.00
R0781:Zfp84	UTSW	7	29,470,797 (GRCm39)	start codon destroyed	probably null	0.02
R1110:Zfp84	UTSW	7	29,470,797 (GRCm39)	start codon destroyed	probably null	0.02
R1353:Zfp84	UTSW	7	29,475,600 (GRCm39)	missense	probably benign	0.02
R1495:Zfp84	UTSW	7	29,476,728 (GRCm39)	nonsense	probably null
R1496:Zfp84	UTSW	7	29,476,039 (GRCm39)	missense	possibly damaging	0.53
R1681:Zfp84	UTSW	7	29,476,825 (GRCm39)	missense	probably damaging	1.00
R1827:Zfp84	UTSW	7	29,476,768 (GRCm39)	missense	possibly damaging	0.91
R1854:Zfp84	UTSW	7	29,474,796 (GRCm39)	missense	possibly damaging	0.84
R2209:Zfp84	UTSW	7	29,476,607 (GRCm39)	missense	probably damaging	0.99
R2843:Zfp84	UTSW	7	29,474,758 (GRCm39)	splice site	probably null
R2844:Zfp84	UTSW	7	29,474,758 (GRCm39)	splice site	probably null
R4691:Zfp84	UTSW	7	29,476,505 (GRCm39)	missense	probably damaging	1.00
R5453:Zfp84	UTSW	7	29,475,722 (GRCm39)	missense	possibly damaging	0.82
R5474:Zfp84	UTSW	7	29,476,514 (GRCm39)	missense	probably damaging	1.00
R5578:Zfp84	UTSW	7	29,474,856 (GRCm39)	missense	possibly damaging	0.93
R5646:Zfp84	UTSW	7	29,475,818 (GRCm39)	missense	probably benign	0.05
R6830:Zfp84	UTSW	7	29,475,911 (GRCm39)	missense	probably benign	0.00
R8129:Zfp84	UTSW	7	29,475,862 (GRCm39)	missense	probably benign	0.00
R8138:Zfp84	UTSW	7	29,474,797 (GRCm39)	missense	probably damaging	0.99
R9180:Zfp84	UTSW	7	29,474,873 (GRCm39)	missense	probably damaging	0.97
R9401:Zfp84	UTSW	7	29,476,297 (GRCm39)	missense	probably damaging	1.00
R9489:Zfp84	UTSW	7	29,476,264 (GRCm39)	missense	possibly damaging	0.89
R9555:Zfp84	UTSW	7	29,476,102 (GRCm39)	missense	probably damaging	0.99
R9605:Zfp84	UTSW	7	29,476,264 (GRCm39)	missense	possibly damaging	0.89
V3553:Zfp84	UTSW	7	29,476,672 (GRCm39)	missense	probably benign	0.36
Z1186:Zfp84	UTSW	7	29,470,805 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GGAAAGCCTTCACCAACTGC -3'
(R):5'- GGTGCAGAATGAGAGTTTTACAATC -3'

Sequencing Primer
(F):5'- CAACTGCTCGCTGCTCG -3'
(R):5'- GGAAGGCCTTCATACACTCCTG -3'

Posted On

2017-03-31