Incidental Mutation 'R5965:Zdhhc24'
ID 472063
Institutional Source Beutler Lab
Gene Symbol Zdhhc24
Ensembl Gene ENSMUSG00000006463
Gene Name zinc finger, DHHC domain containing 24
Synonyms 5730496N17Rik
MMRRC Submission 044150-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5965 (G1)
Quality Score 225
Status Validated
Chromosome 19
Chromosomal Location 4928696-4935425 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to G at 4933778 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 278 (D278E)
Ref Sequence ENSEMBL: ENSMUSP00000006632 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000006632] [ENSMUST00000053506] [ENSMUST00000160014] [ENSMUST00000162720]
AlphaFold Q6IR37
Predicted Effect probably benign
Transcript: ENSMUST00000006632
AA Change: D278E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000006632
Gene: ENSMUSG00000006463
AA Change: D278E

DomainStartEndE-ValueType
transmembrane domain 19 41 N/A INTRINSIC
transmembrane domain 51 73 N/A INTRINSIC
Pfam:zf-DHHC 91 234 4.3e-27 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000053506
SMART Domains Protein: ENSMUSP00000055321
Gene: ENSMUSG00000006464

DomainStartEndE-ValueType
low complexity region 2 11 N/A INTRINSIC
Pfam:BBS1 23 276 2.7e-104 PFAM
low complexity region 293 305 N/A INTRINSIC
low complexity region 458 466 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000160014
SMART Domains Protein: ENSMUSP00000123709
Gene: ENSMUSG00000006463

DomainStartEndE-ValueType
transmembrane domain 19 41 N/A INTRINSIC
transmembrane domain 51 73 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000162088
Predicted Effect noncoding transcript
Transcript: ENSMUST00000162134
Predicted Effect probably benign
Transcript: ENSMUST00000162720
SMART Domains Protein: ENSMUSP00000124337
Gene: ENSMUSG00000006463

DomainStartEndE-ValueType
transmembrane domain 19 41 N/A INTRINSIC
transmembrane domain 51 73 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000162754
Meta Mutation Damage Score 0.0638 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.1%
Validation Efficiency 98% (80/82)
MGI Phenotype PHENOTYPE: Phenotypic analysis of mice homozygous for a gene trap allele indicates this mutation has no notable phenotype in any parameter tested in a high-throughput screen. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgrf3 T A 5: 30,410,637 (GRCm39) T23S probably benign Het
Armc2 A G 10: 41,798,568 (GRCm39) I747T possibly damaging Het
Cacna1c T A 6: 118,579,261 (GRCm39) H1729L probably damaging Het
Crebbp A G 16: 3,905,525 (GRCm39) probably benign Het
Cyp4f17 T A 17: 32,743,611 (GRCm39) M326K probably damaging Het
Dchs1 T G 7: 105,405,132 (GRCm39) D2470A probably damaging Het
Ddhd2 T C 8: 26,225,804 (GRCm39) T518A probably damaging Het
Derl2 T C 11: 70,905,378 (GRCm39) T109A probably benign Het
Dnah7b T C 1: 46,402,147 (GRCm39) L3994P probably damaging Het
Dock10 T C 1: 80,546,461 (GRCm39) probably null Het
Dync1h1 T A 12: 110,599,212 (GRCm39) S1856T probably benign Het
Ehd2 T C 7: 15,685,999 (GRCm39) K358E possibly damaging Het
Enpp2 A G 15: 54,746,367 (GRCm39) probably null Het
Esco1 T C 18: 10,593,867 (GRCm39) E473G possibly damaging Het
Exoc5 A G 14: 49,272,388 (GRCm39) F342S probably damaging Het
Fam186a T A 15: 99,842,978 (GRCm39) T1089S probably benign Het
Fanca T C 8: 124,043,149 (GRCm39) D79G possibly damaging Het
Gabrb2 A G 11: 42,517,696 (GRCm39) Y506C probably damaging Het
Galntl6 T C 8: 58,310,565 (GRCm39) T379A probably benign Het
Garin2 T A 12: 78,757,080 (GRCm39) N12K unknown Het
Gm34768 A G 2: 11,913,316 (GRCm39) noncoding transcript Het
Gps2 A G 11: 69,805,620 (GRCm39) E46G possibly damaging Het
Gsdmc T C 15: 63,676,447 (GRCm39) probably null Het
Gys1 C T 7: 45,104,763 (GRCm39) T666I probably benign Het
Hsd17b11 T A 5: 104,169,651 (GRCm39) probably benign Het
Iffo1 T A 6: 125,129,471 (GRCm39) probably benign Het
Ighv1-51 T C 12: 115,095,177 (GRCm39) noncoding transcript Het
Kansl3 T A 1: 36,384,601 (GRCm39) probably null Het
Kdm3a A G 6: 71,598,364 (GRCm39) I174T probably benign Het
Klhl26 A T 8: 70,905,381 (GRCm39) D95E probably damaging Het
Lair1 T C 7: 4,032,023 (GRCm39) D28G possibly damaging Het
Lama3 A G 18: 12,562,944 (GRCm39) D489G possibly damaging Het
Lamb3 A T 1: 193,025,768 (GRCm39) I1153F probably damaging Het
Lsp1 T A 7: 142,044,161 (GRCm39) probably null Het
Man1a A G 10: 53,809,586 (GRCm39) probably benign Het
Mcam A C 9: 44,047,925 (GRCm39) S57R probably damaging Het
Mrgprf C A 7: 144,861,168 (GRCm39) probably benign Het
Nfia T C 4: 97,999,529 (GRCm39) *499Q probably null Het
Nmbr C A 10: 14,642,554 (GRCm39) R38S probably benign Het
Or12e7 T G 2: 87,288,381 (GRCm39) F291V probably benign Het
Or51a25 C A 7: 102,373,467 (GRCm39) V77L probably benign Het
Or5h23 T C 16: 58,906,666 (GRCm39) Y60C probably damaging Het
P3h1 C T 4: 119,105,424 (GRCm39) H741Y probably benign Het
Parp4 A T 14: 56,861,489 (GRCm39) M941L probably benign Het
Phaf1 T C 8: 105,961,171 (GRCm39) F69L probably damaging Het
Pik3c3 C T 18: 30,431,633 (GRCm39) T331M probably damaging Het
Prkg1 T C 19: 30,701,556 (GRCm39) probably null Het
Qsox2 A T 2: 26,112,233 (GRCm39) V103D probably benign Het
Ranbp1 T C 16: 18,063,092 (GRCm39) T95A probably damaging Het
Ric1 G A 19: 29,548,171 (GRCm39) D280N probably damaging Het
Scd1 A G 19: 44,388,579 (GRCm39) probably null Het
Serpinb3c A G 1: 107,204,653 (GRCm39) I31T probably benign Het
Slc7a11 T A 3: 50,333,593 (GRCm39) Y386F probably benign Het
Smtnl2 A T 11: 72,291,279 (GRCm39) probably null Het
Snx2 G T 18: 53,327,534 (GRCm39) E87* probably null Het
Tars2 A C 3: 95,655,464 (GRCm39) probably null Het
Tax1bp1 C A 6: 52,706,317 (GRCm39) T106N probably damaging Het
Tcof1 A G 18: 60,966,490 (GRCm39) probably null Het
Tenm3 C A 8: 48,681,543 (GRCm39) E2680* probably null Het
Thap7 C T 16: 17,348,611 (GRCm39) probably benign Het
Thoc6 T A 17: 23,889,842 (GRCm39) I23F possibly damaging Het
Tmem185b T G 1: 119,454,294 (GRCm39) Y18* probably null Het
Trav6-1 A C 14: 52,876,254 (GRCm39) Y58S probably damaging Het
Trbv16 A T 6: 41,128,989 (GRCm39) I58F probably benign Het
Ubtfl1 T A 9: 18,320,838 (GRCm39) M122K probably benign Het
Vps13a T C 19: 16,596,392 (GRCm39) probably null Het
Zfp316 T C 5: 143,250,427 (GRCm39) probably null Het
Zfyve9 T C 4: 108,548,878 (GRCm39) T769A possibly damaging Het
Znhit6 T A 3: 145,284,103 (GRCm39) N96K possibly damaging Het
Other mutations in Zdhhc24
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01289:Zdhhc24 APN 19 4,928,850 (GRCm39) missense probably damaging 0.99
PIT4283001:Zdhhc24 UTSW 19 4,928,778 (GRCm39) start codon destroyed probably null 0.01
R0003:Zdhhc24 UTSW 19 4,930,402 (GRCm39) missense possibly damaging 0.51
R1720:Zdhhc24 UTSW 19 4,928,979 (GRCm39) missense probably damaging 0.99
R1780:Zdhhc24 UTSW 19 4,933,794 (GRCm39) missense probably damaging 0.99
R5530:Zdhhc24 UTSW 19 4,933,591 (GRCm39) splice site probably null
R7249:Zdhhc24 UTSW 19 4,928,889 (GRCm39) missense possibly damaging 0.81
Predicted Primers PCR Primer
(F):5'- TTTCCATGGGATGCTGCTGC -3'
(R):5'- TAGACCATCCTCTGCCAAAAGGG -3'

Sequencing Primer
(F):5'- TGCTTCGGGGACAGACCAC -3'
(R):5'- CTACAGAGATAGAAGAGCCCACATTG -3'
Posted On 2017-03-31