Mutagenetix > Incidental Mutations

Incidental Mutation 'R5946:Olfr874'

472182

Institutional Source

Beutler Lab

Gene Symbol

Olfr874

Ensembl Gene

ENSMUSG00000063350

Gene Name

olfactory receptor 874

Synonyms

MOR161-2, GA_x6K02T2PVTD-31428850-31429782

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R5946 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

37745106-37748594 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 37747034 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Glutamine at position 300 (L300Q)

Ref Sequence

ENSEMBL: ENSMUSP00000150088 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000115004] [ENSMUST00000216982]

Predicted Effect

probably damaging
Transcript: ENSMUST00000115004
AA Change: L300Q

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000110656
Gene: ENSMUSG00000063350
AA Change: L300Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	5.9e-49	PFAM
Pfam:7tm_1	40	289	6.2e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000216982
AA Change: L300Q

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc9	A	G	6: 142,625,952	F1137S	probably damaging	Het
Actr6	G	T	10: 89,728,192	Q73K	probably benign	Het
Adamtsl3	G	A	7: 82,576,057	G358D	probably damaging	Het
Aggf1	A	G	13: 95,371,576	V94A	probably damaging	Het
Arpc3	A	G	5: 122,403,396	Y57C	probably damaging	Het
Asb2	A	G	12: 103,321,555	Y630H	probably benign	Het
Atp1a1	A	T	3: 101,589,774	N405K	probably benign	Het
C6	G	T	15: 4,808,514	D869Y	possibly damaging	Het
Cds2	A	G	2: 132,297,248	Y137C	probably damaging	Het
Ceacam12	A	T	7: 18,069,206	E179V	probably damaging	Het
Chgb	T	A	2: 132,792,596	Y153N	probably benign	Het
Cit	T	A	5: 115,997,534	L1831Q	probably damaging	Het
Cpne8	C	A	15: 90,488,988	*578L	probably null	Het
Cspg5	A	G	9: 110,251,083	T440A	probably damaging	Het
Dnah7a	A	C	1: 53,559,308	V1393G	probably damaging	Het
Dnajb8	A	G	6: 88,222,593	D37G	probably benign	Het
Dst	A	G	1: 34,174,192	I1063M	probably benign	Het
Efs	T	G	14: 54,919,494		probably null	Het
Gpatch1	A	G	7: 35,291,832	S596P	probably damaging	Het
Hbs1l	C	A	10: 21,341,756	H190Q	probably benign	Het
Ighm	A	G	12: 113,422,709	V7A	unknown	Het
Ivd	A	T	2: 118,876,889	I295F	possibly damaging	Het
Kcnq5	A	C	1: 21,505,707	S258A	probably damaging	Het
Mad1l1	G	T	5: 140,261,579	P331Q	probably damaging	Het
Mcf2l	G	T	8: 13,013,922	G1045C	probably damaging	Het
Mcoln1	T	A	8: 3,508,701	I233N	probably damaging	Het
Mmp13	T	C	9: 7,276,580	L225P	probably damaging	Het
Muc5ac	G	A	7: 141,817,907	C2615Y	possibly damaging	Het
Myh7b	T	C	2: 155,621,395	F516L	probably damaging	Het
Obsl1	A	T	1: 75,491,207	S1347R	probably damaging	Het
Ogn	A	G	13: 49,618,285	N207S	probably benign	Het
Olfr132	C	A	17: 38,130,707	A162S	probably benign	Het
Pcdha2	G	T	18: 36,941,106	V597L	probably damaging	Het
Pcnt	T	A	10: 76,382,063	Y2126F	possibly damaging	Het
Pgbd5	A	T	8: 124,374,317	M400K	possibly damaging	Het
Pklr	A	T	3: 89,136,196	E5V	probably benign	Het
Pkp4	T	A	2: 59,305,067	D94E	probably benign	Het
Ppan	C	T	9: 20,889,673	Q111*	probably null	Het
Prkcb	A	G	7: 122,544,703	N330S	probably benign	Het
Prl4a1	T	A	13: 28,018,516	W25R	probably damaging	Het
Rars2	T	A	4: 34,656,855	H501Q	possibly damaging	Het
Ryr2	T	C	13: 11,726,953	D2114G	probably damaging	Het
Serinc2	G	T	4: 130,255,521	T351K	possibly damaging	Het
Slc22a12	A	G	19: 6,537,851	F358L	probably damaging	Het
Sorcs2	A	C	5: 36,029,083	V905G	probably damaging	Het
Tekt3	G	C	11: 63,094,747	A460P	probably damaging	Het
Tm4sf1	T	G	3: 57,292,868	I109L	possibly damaging	Het
Tmc5	A	T	7: 118,670,725	E899D	probably damaging	Het
Tmem268	C	T	4: 63,568,509	P90S	probably damaging	Het
Trim38	A	G	13: 23,782,734	M55V	probably benign	Het
Trip10	T	G	17: 57,250,963	V50G	probably damaging	Het
Usp25	T	A	16: 77,115,054	C990*	probably null	Het
Uts2	A	G	4: 150,999,049	D39G	probably benign	Het
Vezf1	T	A	11: 88,073,734	C49*	probably null	Het
Wee2	T	A	6: 40,463,212	N431K	probably null	Het
Yeats2	C	A	16: 20,207,763	Y796*	probably null	Het
Zfp592	G	A	7: 81,037,897	G890D	possibly damaging	Het
Zfp647	G	A	15: 76,912,085	P125L	probably damaging	Het

Other mutations in Olfr874

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Olfr874	APN	9	37746389	missense	possibly damaging	0.89
IGL02349:Olfr874	APN	9	37746206	missense	probably benign	0.03
IGL02799:Olfr874	UTSW	9	37746509	missense	probably damaging	1.00
R0498:Olfr874	UTSW	9	37746254	missense	probably damaging	1.00
R0690:Olfr874	UTSW	9	37746217	missense	probably benign	0.01
R1053:Olfr874	UTSW	9	37746835	missense	probably damaging	0.99
R1777:Olfr874	UTSW	9	37746311	missense	possibly damaging	0.78
R1862:Olfr874	UTSW	9	37746968	missense	probably benign
R1907:Olfr874	UTSW	9	37746433	missense	probably benign	0.35
R4524:Olfr874	UTSW	9	37746866	missense	possibly damaging	0.50
R4731:Olfr874	UTSW	9	37746535	missense	probably benign	0.06
R4746:Olfr874	UTSW	9	37746157	missense	probably benign	0.02
R4768:Olfr874	UTSW	9	37746881	missense	probably damaging	1.00
R5130:Olfr874	UTSW	9	37746509	missense	probably damaging	1.00
R5406:Olfr874	UTSW	9	37746647	missense	probably benign	0.23
R5546:Olfr874	UTSW	9	37746524	missense	probably benign	0.05
R5882:Olfr874	UTSW	9	37746632	missense	probably benign	0.02
R6226:Olfr874	UTSW	9	37746137	start codon destroyed	probably null	1.00
R6705:Olfr874	UTSW	9	37746734	missense	possibly damaging	0.94
R6965:Olfr874	UTSW	9	37746137	start codon destroyed	probably null	1.00
R8008:Olfr874	UTSW	9	37746793	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCAGCTCCCACTTGATTGTG -3'
(R):5'- ATCCATGAAGACCTAGTTTCTCCATAG -3'

Sequencing Primer
(F):5'- CACTTGATTGTGGTTTGTCTTTTC -3'
(R):5'- CTAGTTTCTCCATAGTGCCATAAAC -3'

Posted On

2017-03-31