|Institutional Source||Beutler Lab|
|Gene Name||matrix metallopeptidase 10|
|Is this an essential gene?||Probably non essential (E-score: 0.105)|
|Stock #||R0503 (G1)|
|Chromosomal Location||7502352-7510240 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 7507339 bp|
|Amino Acid Change||Isoleucine to Phenylalanine at position 387 (I387F)|
|Ref Sequence||ENSEMBL: ENSMUSP00000034488 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000034488]|
|Predicted Effect||probably damaging
AA Change: I387F
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: I387F
|Meta Mutation Damage Score||0.7915|
|Coding Region Coverage||
|Validation Efficiency||98% (57/58)|
FUNCTION: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. The lack of encoded protein in mice promotes experimental lung cancer formation, exacerbates experimental colitis and promotes development of inflammation-associated colonic dysplasia. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to bacterial infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Mmp10||
(F):5'- TGACTACAGTTGGCTACGATAACCCAT -3'
(R):5'- GTCAAGTTGAACCTCCACATCGTCTTT -3'
(F):5'- acctgcctctgactccc -3'
(R):5'- CCACATCGTCTTTTGCTTAATCAG -3'