Incidental Mutation 'R3615:Tdo2'
ID473045
Institutional Source Beutler Lab
Gene Symbol Tdo2
Ensembl Gene ENSMUSG00000028011
Gene Nametryptophan 2,3-dioxygenase
Synonymschky, TO, TDO
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.202) question?
Stock #R3615 (G1)
Quality Score225
Status Not validated
Chromosome3
Chromosomal Location81957090-81976202 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 81975428 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Histidine at position 13 (Y13H)
Ref Sequence ENSEMBL: ENSMUSP00000029645 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029645] [ENSMUST00000193879]
Predicted Effect possibly damaging
Transcript: ENSMUST00000029645
AA Change: Y13H

PolyPhen 2 Score 0.684 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000029645
Gene: ENSMUSG00000028011
AA Change: Y13H

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 26 372 8e-177 PFAM
low complexity region 393 406 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000193879
SMART Domains Protein: ENSMUSP00000141237
Gene: ENSMUSG00000028011

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 7 353 1.4e-174 PFAM
low complexity region 374 387 N/A INTRINSIC
Meta Mutation Damage Score 0.0624 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.3%
Validation Efficiency 98% (40/41)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased plasma and brain levels of tryptophan, increased serotonin levels in the brain, decreased anxiety-related behavior, increased neuronal precursor proliferation and accelerated neurogenesis in the granule cell layer of the olfactory bulb. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700022I11Rik A G 4: 42,971,864 N399S probably benign Het
A2ml1 T C 6: 128,558,294 T818A probably benign Het
Aasdh A G 5: 76,888,782 V304A probably benign Het
Angptl3 G A 4: 99,034,465 A248T probably benign Het
Ap2b1 T A 11: 83,324,565 C112S possibly damaging Het
Aqr A T 2: 114,136,887 I549N probably damaging Het
Barhl1 C T 2: 28,911,550 D161N possibly damaging Het
Col28a1 A G 6: 8,014,942 V821A probably damaging Het
Dclk2 G A 3: 86,920,035 P46S probably damaging Het
Dnah1 A G 14: 31,315,148 L247P possibly damaging Het
Dpysl2 T A 14: 66,834,370 H107L probably damaging Het
Dzip3 A G 16: 48,937,063 L869S probably damaging Het
Efs T C 14: 54,920,095 Y160C probably damaging Het
Enam A T 5: 88,504,447 N1197Y possibly damaging Het
Espl1 A G 15: 102,312,989 I944V probably damaging Het
Fam184b A G 5: 45,582,815 V343A possibly damaging Het
Fbxw26 A T 9: 109,743,760 Y105* probably null Het
Fiz1 A G 7: 5,008,172 L449P probably benign Het
Foxi2 T A 7: 135,410,451 C23S possibly damaging Het
Gdf2 G A 14: 33,944,957 R212Q probably damaging Het
Gm5105 C A 3: 138,049,688 A46S unknown Het
Gm597 T C 1: 28,776,575 D792G probably benign Het
Grik5 C T 7: 25,022,571 A581T probably benign Het
Gse1 C G 8: 120,572,742 probably benign Het
Hsp90aa1 T A 12: 110,695,680 M1L possibly damaging Het
Hsp90aa1 C A 12: 110,695,681 probably null Het
Krt25 A C 11: 99,317,298 V368G possibly damaging Het
Lacc1 A G 14: 77,033,287 V269A probably benign Het
Lamc1 T C 1: 153,251,150 K417E probably damaging Het
Miip A G 4: 147,865,914 M75T probably benign Het
Nlrp10 A G 7: 108,924,476 F599S probably benign Het
Nlrp12 T A 7: 3,240,575 M436L probably benign Het
Olfr142 T C 2: 90,252,409 E193G possibly damaging Het
Pafah1b1 G A 11: 74,690,232 S57F probably damaging Het
Pla2g2e G A 4: 138,880,374 V22I probably benign Het
Plekhd1 A G 12: 80,717,270 E202G probably damaging Het
Prss21 A G 17: 23,872,831 T258A probably benign Het
Prss34 A G 17: 25,298,846 E65G probably benign Het
Psap A G 10: 60,294,603 N149S probably benign Het
Ptprf C T 4: 118,237,883 A275T probably benign Het
Sem1 A G 6: 6,578,520 L12P probably damaging Het
Sf3b3 A G 8: 110,844,523 Y4H probably damaging Het
Sh3bp4 G T 1: 89,137,705 R7L probably damaging Het
Slc16a1 T A 3: 104,653,570 L397Q probably damaging Het
Smg5 A G 3: 88,336,451 S10G possibly damaging Het
Tas2r102 C T 6: 132,762,818 Q230* probably null Het
Tmem231 C T 8: 111,918,313 R187H possibly damaging Het
Tmem30b A G 12: 73,545,579 M254T probably damaging Het
Trpm1 G A 7: 64,243,570 G1057R probably damaging Het
Tusc3 A T 8: 39,164,838 K347N probably damaging Het
Usp36 C T 11: 118,276,759 probably null Het
Vash2 T C 1: 190,970,419 Y117C probably damaging Het
Vrk2 A G 11: 26,489,866 I235T possibly damaging Het
Wdr20 A G 12: 110,793,939 T420A probably benign Het
Other mutations in Tdo2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02127:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02129:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02271:Tdo2 APN 3 81963917 splice site probably benign
IGL02686:Tdo2 APN 3 81968155 missense probably benign 0.00
IGL02802:Tdo2 APN 3 81975697 intron probably benign
IGL03171:Tdo2 APN 3 81967029 missense probably benign
IGL03285:Tdo2 APN 3 81958789 unclassified probably null
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0335:Tdo2 UTSW 3 81964000 missense probably benign
R0720:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R1174:Tdo2 UTSW 3 81974376 missense probably damaging 1.00
R1175:Tdo2 UTSW 3 81974376 missense probably damaging 1.00
R1222:Tdo2 UTSW 3 81961468 unclassified probably null
R1418:Tdo2 UTSW 3 81961468 unclassified probably null
R1868:Tdo2 UTSW 3 81960546 missense probably benign 0.04
R1918:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R2031:Tdo2 UTSW 3 81969505 missense probably damaging 1.00
R2513:Tdo2 UTSW 3 81969505 missense possibly damaging 0.91
R3616:Tdo2 UTSW 3 81975428 missense possibly damaging 0.68
R3872:Tdo2 UTSW 3 81968086 missense probably benign 0.08
R5260:Tdo2 UTSW 3 81975323 critical splice donor site probably null
R5547:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R6029:Tdo2 UTSW 3 81961440 missense probably damaging 1.00
R6089:Tdo2 UTSW 3 81962728 missense probably damaging 1.00
R6163:Tdo2 UTSW 3 81975403 missense possibly damaging 0.49
R6379:Tdo2 UTSW 3 81958795 unclassified probably benign
R7060:Tdo2 UTSW 3 81969559 missense probably damaging 1.00
R7544:Tdo2 UTSW 3 81971635 critical splice donor site probably null
R7585:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R7724:Tdo2 UTSW 3 81968083 critical splice donor site probably null
Predicted Primers
Posted On2017-04-14