Mutagenetix > Incidental Mutation

Incidental Mutation 'R0504:Mdn1'

47313

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, D4Abb1e, LOC213784

MMRRC Submission

038699-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0504 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 32698916 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000136222 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000178134]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000071642

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136608

Predicted Effect

probably benign
Transcript: ENSMUST00000178134

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.6%

Validation Efficiency

99% (145/147)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 145 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921539E11Rik	A	G	4: 103,128,057 (GRCm39)		probably benign	Het
Adamts6	T	C	13: 104,563,438 (GRCm39)		probably benign	Het
Adamts9	T	A	6: 92,889,626 (GRCm39)	Y316F	probably damaging	Het
Agl	A	T	3: 116,580,433 (GRCm39)	F374I	probably damaging	Het
Akr1c19	A	G	13: 4,286,250 (GRCm39)	T83A	possibly damaging	Het
Ankrd36	T	A	11: 5,579,274 (GRCm39)	S179R	probably damaging	Het
Appbp2	A	G	11: 85,082,513 (GRCm39)	S573P	probably benign	Het
Arid4a	T	A	12: 71,093,988 (GRCm39)	F254I	probably damaging	Het
Bin3	T	C	14: 70,361,336 (GRCm39)		probably null	Het
Bmi1	T	C	2: 18,688,883 (GRCm39)		probably null	Het
Bmper	G	T	9: 23,317,983 (GRCm39)	C534F	probably damaging	Het
Bora	T	A	14: 99,299,059 (GRCm39)	C205*	probably null	Het
Btnl2	A	G	17: 34,577,091 (GRCm39)	E82G	probably benign	Het
Ccdc8	A	T	7: 16,729,939 (GRCm39)	D476V	unknown	Het
Ccr3	C	A	9: 123,829,478 (GRCm39)	T271K	possibly damaging	Het
Cd276	A	G	9: 58,447,961 (GRCm39)	L23P	possibly damaging	Het
Cd3e	T	C	9: 44,913,552 (GRCm39)	Q61R	probably benign	Het
Cep97	A	G	16: 55,726,142 (GRCm39)	S582P	probably benign	Het
Chml	A	T	1: 175,514,748 (GRCm39)	M391K	probably damaging	Het
Chst1	A	G	2: 92,444,169 (GRCm39)	N214D	probably benign	Het
Chuk	T	C	19: 44,070,377 (GRCm39)		probably benign	Het
Col12a1	G	A	9: 79,588,750 (GRCm39)	H1122Y	possibly damaging	Het
Cpne6	A	G	14: 55,752,059 (GRCm39)	K272R	probably damaging	Het
Cpsf2	T	A	12: 101,956,262 (GRCm39)	L355Q	probably damaging	Het
Cyp2c29	T	A	19: 39,298,224 (GRCm39)	D256E	probably benign	Het
Daglb	G	A	5: 143,479,952 (GRCm39)	V420I	probably benign	Het
Ddx42	G	T	11: 106,138,675 (GRCm39)	G825C	probably benign	Het
Dis3	T	C	14: 99,318,826 (GRCm39)		probably benign	Het
Dkk4	C	T	8: 23,115,359 (GRCm39)	R70C	probably damaging	Het
Dock6	G	T	9: 21,713,732 (GRCm39)	Q1933K	probably damaging	Het
Dpep2	T	G	8: 106,716,620 (GRCm39)	Q186H	probably benign	Het
Dzip3	A	C	16: 48,780,006 (GRCm39)		probably benign	Het
Egflam	T	A	15: 7,252,239 (GRCm39)	I853F	probably damaging	Het
Fastkd5	A	G	2: 130,457,837 (GRCm39)	I251T	probably benign	Het
Fbn2	T	A	18: 58,172,532 (GRCm39)	D2091V	possibly damaging	Het
Fem1al	T	C	11: 29,774,990 (GRCm39)	I156V	probably damaging	Het
Fer1l4	C	A	2: 155,894,115 (GRCm39)	V63L	probably benign	Het
Frem1	T	A	4: 82,830,874 (GRCm39)	D2062V	probably benign	Het
Galnt6	A	C	15: 100,594,538 (GRCm39)		probably benign	Het
Get3	A	C	8: 85,745,236 (GRCm39)	V277G	probably damaging	Het
Gm10972	A	T	3: 94,550,440 (GRCm39)		probably benign	Het
Gm4846	G	A	1: 166,319,114 (GRCm39)	T208I	probably benign	Het
Gorab	A	G	1: 163,214,174 (GRCm39)	L252P	probably damaging	Het
Gtsf2	A	G	15: 103,352,988 (GRCm39)	C63R	probably damaging	Het
Hal	T	C	10: 93,325,036 (GRCm39)	V15A	probably damaging	Het
Hmcn1	A	T	1: 150,752,170 (GRCm39)		probably benign	Het
Hormad2	A	T	11: 4,358,833 (GRCm39)	H191Q	possibly damaging	Het
Hspa2	A	T	12: 76,451,990 (GRCm39)	D228V	probably damaging	Het
Igfn1	A	T	1: 135,896,267 (GRCm39)	M1433K	probably benign	Het
Il18	A	G	9: 50,486,628 (GRCm39)	D19G	probably damaging	Het
Il1rl2	G	A	1: 40,368,216 (GRCm39)	V129I	probably benign	Het
Inpp5b	C	A	4: 124,676,201 (GRCm39)	Y352*	probably null	Het
Insrr	A	T	3: 87,720,463 (GRCm39)	M1034L	possibly damaging	Het
Jmjd1c	T	C	10: 67,061,534 (GRCm39)	S1296P	probably damaging	Het
Kdm5b	G	T	1: 134,548,761 (GRCm39)		probably null	Het
Krba1	C	T	6: 48,393,188 (GRCm39)	T998I	probably benign	Het
L3mbtl4	A	G	17: 69,084,907 (GRCm39)	N606S	probably benign	Het
Lonrf1	T	A	8: 36,698,313 (GRCm39)	N395I	possibly damaging	Het
Lpp	A	G	16: 24,790,720 (GRCm39)	D393G	probably damaging	Het
Lrrc17	A	G	5: 21,765,528 (GRCm39)	I3M	probably benign	Het
Lrrtm4	A	T	6: 79,999,029 (GRCm39)	Q147L	probably damaging	Het
Map1a	A	G	2: 121,133,422 (GRCm39)	M1413V	probably benign	Het
Mapk8ip2	A	G	15: 89,340,861 (GRCm39)	E102G	possibly damaging	Het
Marf1	C	T	16: 13,960,398 (GRCm39)	A549T	probably damaging	Het
Mfng	A	C	15: 78,641,514 (GRCm39)	H294Q	probably benign	Het
Mical2	T	A	7: 111,870,524 (GRCm39)	N4K	probably benign	Het
Mov10l1	T	A	15: 88,883,042 (GRCm39)	V384E	probably damaging	Het
Myo18b	A	G	5: 113,021,442 (GRCm39)		probably benign	Het
Nlrp1b	T	G	11: 71,073,241 (GRCm39)	I201L	probably damaging	Het
Nos2	C	T	11: 78,830,903 (GRCm39)	P249L	probably damaging	Het
Notch4	A	G	17: 34,794,065 (GRCm39)	T681A	probably damaging	Het
Nr1i3	C	T	1: 171,044,805 (GRCm39)		probably benign	Het
Obscn	A	G	11: 58,899,333 (GRCm39)		probably null	Het
Onecut2	A	T	18: 64,473,820 (GRCm39)	I124F	possibly damaging	Het
Or10a49	C	T	7: 108,468,057 (GRCm39)	M101I	possibly damaging	Het
Or2a14	A	T	6: 43,130,395 (GRCm39)	H52L	probably benign	Het
Or2ag1	T	A	7: 106,313,908 (GRCm39)		probably benign	Het
Or2f2	C	T	6: 42,767,530 (GRCm39)	R186*	probably null	Het
Or2t47	C	A	11: 58,442,462 (GRCm39)	C201F	probably damaging	Het
Or4a75	A	T	2: 89,448,438 (GRCm39)	Y33N	probably damaging	Het
Or4c123	C	T	2: 89,127,083 (GRCm39)	C177Y	probably damaging	Het
Or52u1	C	T	7: 104,237,682 (GRCm39)	R224*	probably null	Het
Or5m12	T	A	2: 85,735,030 (GRCm39)	M123L	possibly damaging	Het
Or8b35	A	G	9: 37,904,438 (GRCm39)	T217A	probably benign	Het
Otol1	G	A	3: 69,934,937 (GRCm39)	G310R	probably damaging	Het
Oxct2b	T	A	4: 123,010,633 (GRCm39)	S184R	possibly damaging	Het
Oxct2b	ACTG	A	4: 123,010,705 (GRCm39)		probably benign	Het
P2rx6	A	G	16: 17,385,291 (GRCm39)		probably benign	Het
Pde4a	A	G	9: 21,115,699 (GRCm39)	N411S	probably damaging	Het
Phkb	A	T	8: 86,783,153 (GRCm39)	D983V	probably benign	Het
Piezo2	G	A	18: 63,157,522 (GRCm39)	T2396I	probably damaging	Het
Pik3ap1	T	A	19: 41,275,929 (GRCm39)	D717V	probably damaging	Het
Plce1	T	A	19: 38,766,465 (GRCm39)		probably benign	Het
Plekhg1	A	G	10: 3,887,853 (GRCm39)	I261V	probably damaging	Het
Ppfia4	T	C	1: 134,251,851 (GRCm39)	H441R	probably damaging	Het
Prpf8	T	A	11: 75,392,768 (GRCm39)		probably benign	Het
Ptn	T	A	6: 36,718,388 (GRCm39)		probably benign	Het
Ptpn13	A	G	5: 103,649,362 (GRCm39)	Y255C	possibly damaging	Het
Ptpn4	A	G	1: 119,693,645 (GRCm39)	Y126H	probably damaging	Het
Ptprc	T	C	1: 138,016,435 (GRCm39)	N505D	probably damaging	Het
Ptprs	T	A	17: 56,761,220 (GRCm39)	I116F	possibly damaging	Het
Rab1a	T	G	11: 20,173,169 (GRCm39)	V90G	probably damaging	Het
Rcor1	T	C	12: 111,068,102 (GRCm39)	V267A	probably benign	Het
Reep4	A	G	14: 70,784,678 (GRCm39)		probably null	Het
Rere	T	A	4: 150,699,779 (GRCm39)		probably benign	Het
Rin3	T	A	12: 102,353,823 (GRCm39)	Y743*	probably null	Het
Rprm	A	G	2: 53,975,067 (GRCm39)	S84P	probably damaging	Het
Sdhaf2	C	T	19: 10,494,383 (GRCm39)	E109K	probably damaging	Het
Sec31b	G	T	19: 44,523,225 (GRCm39)	Q24K	probably damaging	Het
Sema5a	T	C	15: 32,574,949 (GRCm39)		probably benign	Het
Sh3pxd2a	T	C	19: 47,256,186 (GRCm39)	Y844C	probably damaging	Het
Shmt2	A	C	10: 127,355,941 (GRCm39)	N134K	probably damaging	Het
Slc9a8	C	T	2: 167,266,125 (GRCm39)	A34V	probably benign	Het
Spidr	A	C	16: 15,957,936 (GRCm39)	S64A	possibly damaging	Het
Stk10	A	G	11: 32,567,882 (GRCm39)	T895A	probably benign	Het
Syne2	A	T	12: 76,080,365 (GRCm39)		probably benign	Het
Szt2	T	C	4: 118,230,149 (GRCm39)		probably null	Het
Tecpr1	A	T	5: 144,150,899 (GRCm39)	V303D	probably damaging	Het
Tet3	A	G	6: 83,350,776 (GRCm39)	Y1048H	probably damaging	Het
Tfb2m	A	G	1: 179,373,396 (GRCm39)	C101R	probably damaging	Het
Tg	T	C	15: 66,554,253 (GRCm39)	V556A	probably damaging	Het
Thbs4	A	C	13: 92,903,692 (GRCm39)	I441M	probably benign	Het
Thsd7a	A	T	6: 12,379,593 (GRCm39)	Y944N	probably damaging	Het
Tm9sf3	C	A	19: 41,236,331 (GRCm39)		probably benign	Het
Tmem145	T	C	7: 25,010,787 (GRCm39)	F359S	probably damaging	Het
Ttc21b	C	T	2: 66,053,142 (GRCm39)		probably benign	Het
Ttn	A	G	2: 76,579,880 (GRCm39)	V23671A	probably damaging	Het
Txnl4b	T	C	8: 110,298,103 (GRCm39)	I78T	probably benign	Het
Ubr4	C	A	4: 139,133,889 (GRCm39)	L762I	probably damaging	Het
Ubr4	T	C	4: 139,208,149 (GRCm39)		probably null	Het
Ugt1a8	C	T	1: 88,016,079 (GRCm39)	P164L	probably damaging	Het
Unc13b	T	C	4: 43,263,559 (GRCm39)	S1594P	probably damaging	Het
Utrn	A	T	10: 12,278,639 (GRCm39)	F912I	probably benign	Het
Vat1l	T	C	8: 114,963,319 (GRCm39)		probably benign	Het
Vmn1r50	T	A	6: 90,084,863 (GRCm39)	S203T	probably damaging	Het
Vmn2r4	A	T	3: 64,296,784 (GRCm39)	L667Q	probably damaging	Het
Vmn2r66	T	G	7: 84,656,023 (GRCm39)	Q331P	probably damaging	Het
Wdsub1	A	T	2: 59,708,669 (GRCm39)	V68D	possibly damaging	Het
Wnk2	C	G	13: 49,238,870 (GRCm39)	A564P	possibly damaging	Het
Wnk2	T	A	13: 49,238,872 (GRCm39)	K563M	probably damaging	Het
Zan	T	A	5: 137,468,580 (GRCm39)	H297L	probably damaging	Het
Zfp426	A	T	9: 20,381,327 (GRCm39)	H539Q	probably damaging	Het
Zfp488	T	A	14: 33,692,497 (GRCm39)	N222I	probably damaging	Het
Zfp536	T	A	7: 37,268,243 (GRCm39)	H391L	probably damaging	Het
Zp1	T	A	19: 10,893,571 (GRCm39)	N31I	probably damaging	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32,723,651 (GRCm39)	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32,719,214 (GRCm39)	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32,735,719 (GRCm39)	missense	probably benign
IGL00573:Mdn1	APN	4	32,666,619 (GRCm39)	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32,735,525 (GRCm39)	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32,730,864 (GRCm39)	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32,743,686 (GRCm39)	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32,715,922 (GRCm39)	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32,711,938 (GRCm39)	splice site	probably benign
IGL01684:Mdn1	APN	4	32,726,857 (GRCm39)	missense	probably benign
IGL01753:Mdn1	APN	4	32,708,483 (GRCm39)	missense	probably benign
IGL01901:Mdn1	APN	4	32,669,591 (GRCm39)	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32,723,657 (GRCm39)	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32,758,393 (GRCm39)	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32,749,948 (GRCm39)	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32,715,708 (GRCm39)	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32,709,364 (GRCm39)	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32,740,395 (GRCm39)	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32,739,092 (GRCm39)	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32,676,860 (GRCm39)	splice site	probably benign
IGL02396:Mdn1	APN	4	32,700,120 (GRCm39)	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32,694,674 (GRCm39)	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32,670,579 (GRCm39)	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32,763,199 (GRCm39)	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32,734,366 (GRCm39)	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32,713,360 (GRCm39)	splice site	probably benign
IGL03076:Mdn1	APN	4	32,735,564 (GRCm39)	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32,729,994 (GRCm39)	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32,732,842 (GRCm39)	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32,725,967 (GRCm39)	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32,694,495 (GRCm39)	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32,749,934 (GRCm39)	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32,719,223 (GRCm39)	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32,746,527 (GRCm39)	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32,718,317 (GRCm39)	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32,729,956 (GRCm39)	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32,693,534 (GRCm39)	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32,750,318 (GRCm39)	missense	probably damaging	1.00
R0362:Mdn1	UTSW	4	32,746,439 (GRCm39)	critical splice acceptor site	probably null
R0421:Mdn1	UTSW	4	32,684,707 (GRCm39)	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32,699,204 (GRCm39)	splice site	probably benign
R0469:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0477:Mdn1	UTSW	4	32,750,928 (GRCm39)	missense	probably benign	0.02
R0481:Mdn1	UTSW	4	32,767,182 (GRCm39)	splice site	probably benign
R0522:Mdn1	UTSW	4	32,672,837 (GRCm39)	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32,730,479 (GRCm39)	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32,732,829 (GRCm39)	missense	probably benign	0.36
R0607:Mdn1	UTSW	4	32,712,014 (GRCm39)	missense	probably damaging	1.00
R0664:Mdn1	UTSW	4	32,768,011 (GRCm39)	nonsense	probably null
R0701:Mdn1	UTSW	4	32,699,263 (GRCm39)	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32,668,895 (GRCm39)	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32,752,032 (GRCm39)	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32,701,713 (GRCm39)	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32,746,568 (GRCm39)	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32,694,511 (GRCm39)	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32,667,089 (GRCm39)	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32,700,334 (GRCm39)	splice site	probably benign
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32,739,977 (GRCm39)	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32,723,501 (GRCm39)	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1591:Mdn1	UTSW	4	32,700,092 (GRCm39)	missense	possibly damaging	0.65
R1678:Mdn1	UTSW	4	32,663,050 (GRCm39)	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32,700,417 (GRCm39)	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32,773,952 (GRCm39)	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32,700,103 (GRCm39)	missense	probably damaging	1.00
R1833:Mdn1	UTSW	4	32,720,761 (GRCm39)	missense	probably damaging	0.97
R1858:Mdn1	UTSW	4	32,730,881 (GRCm39)	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32,763,339 (GRCm39)	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32,742,540 (GRCm39)	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32,760,839 (GRCm39)	small deletion	probably benign
R2075:Mdn1	UTSW	4	32,716,058 (GRCm39)	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32,738,712 (GRCm39)	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32,743,843 (GRCm39)	splice site	probably null
R2110:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32,716,271 (GRCm39)	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32,763,306 (GRCm39)	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32,765,701 (GRCm39)	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32,750,010 (GRCm39)	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32,723,621 (GRCm39)	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32,750,013 (GRCm39)	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32,720,818 (GRCm39)	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32,693,506 (GRCm39)	nonsense	probably null
R3973:Mdn1	UTSW	4	32,722,363 (GRCm39)	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32,707,475 (GRCm39)	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32,743,809 (GRCm39)	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32,754,482 (GRCm39)	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32,704,635 (GRCm39)	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32,668,860 (GRCm39)	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32,715,883 (GRCm39)	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32,722,334 (GRCm39)	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32,754,437 (GRCm39)	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32,741,812 (GRCm39)	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32,707,636 (GRCm39)	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32,730,749 (GRCm39)	splice site	probably null
R4667:Mdn1	UTSW	4	32,679,572 (GRCm39)	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32,666,430 (GRCm39)	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32,683,583 (GRCm39)	nonsense	probably null
R4807:Mdn1	UTSW	4	32,685,651 (GRCm39)	splice site	probably null
R4923:Mdn1	UTSW	4	32,671,608 (GRCm39)	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32,707,459 (GRCm39)	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32,756,512 (GRCm39)	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32,739,827 (GRCm39)	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32,734,418 (GRCm39)	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32,670,593 (GRCm39)	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32,774,008 (GRCm39)	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32,759,011 (GRCm39)	splice site	probably null
R5215:Mdn1	UTSW	4	32,741,418 (GRCm39)	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32,720,897 (GRCm39)	missense	probably benign
R5522:Mdn1	UTSW	4	32,685,783 (GRCm39)	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32,767,961 (GRCm39)	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32,728,167 (GRCm39)	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32,765,664 (GRCm39)	missense	probably benign
R5621:Mdn1	UTSW	4	32,716,371 (GRCm39)	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32,695,480 (GRCm39)	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32,667,467 (GRCm39)	splice site	probably null
R5780:Mdn1	UTSW	4	32,722,950 (GRCm39)	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32,754,547 (GRCm39)	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32,670,646 (GRCm39)	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32,695,400 (GRCm39)	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32,678,330 (GRCm39)	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32,741,073 (GRCm39)	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32,715,713 (GRCm39)	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32,689,581 (GRCm39)	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32,684,735 (GRCm39)	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32,716,040 (GRCm39)	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32,715,953 (GRCm39)	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32,696,269 (GRCm39)	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32,708,484 (GRCm39)	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32,748,590 (GRCm39)	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32,749,593 (GRCm39)	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32,715,979 (GRCm39)	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32,730,054 (GRCm39)	nonsense	probably null
R6384:Mdn1	UTSW	4	32,670,607 (GRCm39)	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32,773,308 (GRCm39)	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32,713,780 (GRCm39)	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32,676,786 (GRCm39)	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32,741,893 (GRCm39)	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32,748,614 (GRCm39)	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32,726,942 (GRCm39)	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32,733,374 (GRCm39)	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32,767,969 (GRCm39)	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32,762,341 (GRCm39)	missense	probably benign
R7158:Mdn1	UTSW	4	32,725,121 (GRCm39)	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32,746,446 (GRCm39)	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32,719,184 (GRCm39)	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32,694,634 (GRCm39)	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32,701,823 (GRCm39)	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32,695,427 (GRCm39)	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32,725,944 (GRCm39)	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32,723,685 (GRCm39)	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32,691,729 (GRCm39)	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32,773,375 (GRCm39)	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32,667,270 (GRCm39)	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32,696,359 (GRCm39)	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32,694,599 (GRCm39)	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32,691,229 (GRCm39)	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32,739,912 (GRCm39)	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32,722,360 (GRCm39)	missense	possibly damaging	0.75
R7718:Mdn1	UTSW	4	32,718,420 (GRCm39)	missense	probably damaging	1.00
R7762:Mdn1	UTSW	4	32,734,421 (GRCm39)	missense	probably benign
R7787:Mdn1	UTSW	4	32,741,794 (GRCm39)	missense	probably damaging	1.00
R8111:Mdn1	UTSW	4	32,674,003 (GRCm39)	missense	possibly damaging	0.81
R8222:Mdn1	UTSW	4	32,707,477 (GRCm39)	missense	probably benign	0.09
R8246:Mdn1	UTSW	4	32,657,284 (GRCm39)	missense	probably benign	0.06
R8267:Mdn1	UTSW	4	32,742,485 (GRCm39)	missense	possibly damaging	0.82
R8286:Mdn1	UTSW	4	32,731,960 (GRCm39)	missense	possibly damaging	0.91
R8305:Mdn1	UTSW	4	32,725,107 (GRCm39)	missense	probably benign
R8318:Mdn1	UTSW	4	32,735,897 (GRCm39)	critical splice donor site	probably null
R8379:Mdn1	UTSW	4	32,756,453 (GRCm39)	missense	probably null	1.00
R8384:Mdn1	UTSW	4	32,765,680 (GRCm39)	missense	probably benign	0.05
R8514:Mdn1	UTSW	4	32,739,857 (GRCm39)	missense	probably damaging	1.00
R8560:Mdn1	UTSW	4	32,743,830 (GRCm39)	missense	probably benign	0.08
R8672:Mdn1	UTSW	4	32,768,793 (GRCm39)	missense	probably damaging	1.00
R8708:Mdn1	UTSW	4	32,725,854 (GRCm39)	missense	probably damaging	1.00
R8769:Mdn1	UTSW	4	32,751,390 (GRCm39)	missense	probably damaging	0.97
R8896:Mdn1	UTSW	4	32,678,328 (GRCm39)	missense	probably benign	0.28
R8918:Mdn1	UTSW	4	32,744,579 (GRCm39)	nonsense	probably null
R8920:Mdn1	UTSW	4	32,719,280 (GRCm39)	missense	probably damaging	1.00
R8966:Mdn1	UTSW	4	32,672,837 (GRCm39)	nonsense	probably null
R8997:Mdn1	UTSW	4	32,773,275 (GRCm39)	missense	probably damaging	1.00
R9120:Mdn1	UTSW	4	32,701,814 (GRCm39)	missense	probably damaging	1.00
R9129:Mdn1	UTSW	4	32,676,812 (GRCm39)	missense	probably benign	0.24
R9131:Mdn1	UTSW	4	32,762,275 (GRCm39)	missense	possibly damaging	0.69
R9200:Mdn1	UTSW	4	32,760,791 (GRCm39)	missense	probably benign	0.00
R9226:Mdn1	UTSW	4	32,694,612 (GRCm39)	missense	probably benign	0.25
R9235:Mdn1	UTSW	4	32,739,122 (GRCm39)	missense	probably benign	0.10
R9293:Mdn1	UTSW	4	32,707,579 (GRCm39)	missense	probably damaging	1.00
R9315:Mdn1	UTSW	4	32,760,911 (GRCm39)	missense	probably benign	0.00
R9338:Mdn1	UTSW	4	32,666,536 (GRCm39)	missense	probably benign	0.00
R9353:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R9393:Mdn1	UTSW	4	32,713,825 (GRCm39)	missense
R9420:Mdn1	UTSW	4	32,678,414 (GRCm39)	missense	probably damaging	1.00
R9475:Mdn1	UTSW	4	32,739,849 (GRCm39)	missense	possibly damaging	0.65
R9583:Mdn1	UTSW	4	32,741,372 (GRCm39)	missense	probably damaging	1.00
R9600:Mdn1	UTSW	4	32,684,723 (GRCm39)	nonsense	probably null
R9640:Mdn1	UTSW	4	32,754,539 (GRCm39)	missense	probably damaging	1.00
R9688:Mdn1	UTSW	4	32,745,590 (GRCm39)	missense	probably damaging	1.00
R9744:Mdn1	UTSW	4	32,715,711 (GRCm39)	missense	possibly damaging	0.91
X0066:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,696,244 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,668,944 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,667,102 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTCCTAGTCCTACCGGAGACGGTC -3'
(R):5'- TGCCTGCCAGAAGCATGAAGCC -3'

Sequencing Primer
(F):5'- CCTCGGTGTTTGCTGGAAAG -3'
(R):5'- GTAAGAGTGACTGTTACCCACTG -3'

Posted On

2013-06-12