Mutagenetix > Incidental Mutation

Incidental Mutation 'R0505:Robo3'

47485

Institutional Source

Beutler Lab

Gene Symbol

Robo3

Ensembl Gene

ENSMUSG00000032128

Gene Name

roundabout guidance receptor 3

Synonyms

Robo3a, Rbig1, Rig1, Rig-1, Robo3b

MMRRC Submission

038700-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0505 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

37327341-37344730 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to A at 37328055 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000150722 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034643] [ENSMUST00000102895] [ENSMUST00000115038] [ENSMUST00000115046] [ENSMUST00000115048] [ENSMUST00000156972] [ENSMUST00000170512] [ENSMUST00000214185]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000034643
AA Change: R1357C

SMART Domains

Protein: ENSMUSP00000034643
Gene: ENSMUSG00000032128
AA Change: R1357C

Domain	Start	End	E-Value	Type
IGc2	54	128	9.7e-11	SMART
IGc2	156	221	1.44e-4	SMART
IGc2	248	311	1.89e-13	SMART
IGc2	337	409	9.84e-12	SMART
IGc2	441	506	2.09e-15	SMART
FN3	534	616	4.24e-14	SMART
FN3	648	731	3.06e0	SMART
FN3	747	832	1.97e-9	SMART
low complexity region	870	890	N/A	INTRINSIC
low complexity region	1055	1082	N/A	INTRINSIC
low complexity region	1131	1149	N/A	INTRINSIC
low complexity region	1155	1169	N/A	INTRINSIC
low complexity region	1193	1206	N/A	INTRINSIC
low complexity region	1245	1256	N/A	INTRINSIC
low complexity region	1268	1281	N/A	INTRINSIC
low complexity region	1336	1376	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000102895

SMART Domains

Protein: ENSMUSP00000099959
Gene: ENSMUSG00000032125

Domain	Start	End	E-Value	Type
signal peptide	1	37	N/A	INTRINSIC
IG	48	144	2.51e0	SMART
IGc2	160	225	6.86e-11	SMART
FN3	263	343	2.05e0	SMART
FN3	358	440	1.27e-3	SMART
low complexity region	488	494	N/A	INTRINSIC
low complexity region	544	562	N/A	INTRINSIC
low complexity region	720	733	N/A	INTRINSIC
low complexity region	748	762	N/A	INTRINSIC
low complexity region	775	799	N/A	INTRINSIC
low complexity region	855	866	N/A	INTRINSIC
low complexity region	871	880	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000115038
AA Change: R1379C

SMART Domains

Protein: ENSMUSP00000110690
Gene: ENSMUSG00000032128
AA Change: R1379C

Domain	Start	End	E-Value	Type
low complexity region	34	47	N/A	INTRINSIC
IGc2	76	150	9.7e-11	SMART
IGc2	178	243	1.44e-4	SMART
IGc2	270	333	1.89e-13	SMART
IGc2	359	431	9.84e-12	SMART
IGc2	463	528	2.09e-15	SMART
FN3	556	638	4.24e-14	SMART
FN3	670	753	3.06e0	SMART
FN3	769	854	1.97e-9	SMART
low complexity region	892	912	N/A	INTRINSIC
low complexity region	1077	1104	N/A	INTRINSIC
low complexity region	1153	1171	N/A	INTRINSIC
low complexity region	1177	1191	N/A	INTRINSIC
low complexity region	1215	1228	N/A	INTRINSIC
low complexity region	1267	1278	N/A	INTRINSIC
low complexity region	1290	1303	N/A	INTRINSIC
low complexity region	1358	1398	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000115046

SMART Domains

Protein: ENSMUSP00000110698
Gene: ENSMUSG00000032125

Domain	Start	End	E-Value	Type
signal peptide	1	37	N/A	INTRINSIC
IG	48	144	2.51e0	SMART
IGc2	160	225	6.86e-11	SMART
FN3	263	343	2.05e0	SMART
FN3	358	440	1.27e-3	SMART
low complexity region	484	500	N/A	INTRINSIC
low complexity region	540	546	N/A	INTRINSIC
low complexity region	596	614	N/A	INTRINSIC
low complexity region	747	756	N/A	INTRINSIC
low complexity region	779	792	N/A	INTRINSIC
low complexity region	807	821	N/A	INTRINSIC
low complexity region	834	858	N/A	INTRINSIC
low complexity region	914	925	N/A	INTRINSIC
low complexity region	930	939	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000115048

SMART Domains

Protein: ENSMUSP00000110700
Gene: ENSMUSG00000032125

Domain	Start	End	E-Value	Type
signal peptide	1	37	N/A	INTRINSIC
IG	48	144	2.51e0	SMART
IGc2	160	225	6.86e-11	SMART
FN3	263	343	2.05e0	SMART
FN3	358	440	1.27e-3	SMART
low complexity region	488	494	N/A	INTRINSIC
low complexity region	544	562	N/A	INTRINSIC
low complexity region	695	704	N/A	INTRINSIC
low complexity region	727	740	N/A	INTRINSIC
low complexity region	755	769	N/A	INTRINSIC
low complexity region	782	806	N/A	INTRINSIC
low complexity region	862	873	N/A	INTRINSIC
low complexity region	878	887	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000156972

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167089

Predicted Effect

probably benign
Transcript: ENSMUST00000170512

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000171467

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167216

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215777

Predicted Effect

probably benign
Transcript: ENSMUST00000214185

Meta Mutation Damage Score

0.0798

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

98% (119/121)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
PHENOTYPE: Homozygous mutants display perinatal lethality, abnormal commissural axon growth, and fragile floor plates. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,241,058 (GRCm39)	Y974H	probably benign	Het
Abca2	G	T	2: 25,324,906 (GRCm39)	G300V	probably benign	Het
Abi1	A	G	2: 22,852,516 (GRCm39)		probably benign	Het
Actr10	T	A	12: 71,006,738 (GRCm39)	Y332N	probably damaging	Het
Adam25	G	T	8: 41,208,261 (GRCm39)	C509F	probably damaging	Het
Adck1	A	T	12: 88,338,461 (GRCm39)		probably benign	Het
Adgra3	A	G	5: 50,166,676 (GRCm39)		probably null	Het
Adgrl1	G	T	8: 84,661,279 (GRCm39)		probably benign	Het
Akr1c21	A	G	13: 4,626,306 (GRCm39)	Y110C	probably damaging	Het
Arhgef25	T	C	10: 127,019,566 (GRCm39)	I463V	probably null	Het
Atp6v1e2	C	T	17: 87,252,006 (GRCm39)	V131M	probably benign	Het
Bdnf	A	G	2: 109,505,688 (GRCm39)		probably null	Het
Bltp3b	T	C	10: 89,627,305 (GRCm39)	S145P	probably damaging	Het
C7	A	T	15: 5,023,624 (GRCm39)		probably benign	Het
Cdc27	T	C	11: 104,419,114 (GRCm39)	T273A	probably benign	Het
Cdo1	T	A	18: 46,848,678 (GRCm39)	I187F	probably benign	Het
Cep104	A	T	4: 154,080,761 (GRCm39)	T742S	probably benign	Het
Ckm	A	T	7: 19,153,377 (GRCm39)	K223*	probably null	Het
Cmtr1	C	T	17: 29,895,259 (GRCm39)	P586L	probably benign	Het
Csmd1	C	T	8: 16,042,758 (GRCm39)	R2325Q	probably damaging	Het
Dcpp1	A	T	17: 24,101,568 (GRCm39)	I106L	possibly damaging	Het
Diaph3	A	C	14: 87,328,400 (GRCm39)		probably benign	Het
Dnah11	A	G	12: 118,070,245 (GRCm39)	V1520A	probably damaging	Het
Dnajc25	T	A	4: 59,020,438 (GRCm39)	M168K		Het
Dpp3	T	C	19: 4,964,682 (GRCm39)	N542D	probably damaging	Het
Ebf2	A	T	14: 67,609,185 (GRCm39)	K199*	probably null	Het
Eeig2	T	C	3: 108,887,520 (GRCm39)	E248G	probably benign	Het
Efcab11	T	A	12: 99,685,294 (GRCm39)	Q160L	probably benign	Het
Eif2ak4	T	A	2: 118,261,517 (GRCm39)	S686T	probably benign	Het
Epha6	C	T	16: 60,026,095 (GRCm39)	S449N	possibly damaging	Het
Ercc4	T	C	16: 12,944,331 (GRCm39)	V329A	probably benign	Het
Faf1	T	C	4: 109,697,600 (GRCm39)	F309L	possibly damaging	Het
G6pd2	C	A	5: 61,966,910 (GRCm39)	D228E	probably benign	Het
Ggt1	T	G	10: 75,421,791 (GRCm39)	V546G	probably damaging	Het
Gpatch4	G	T	3: 87,958,524 (GRCm39)	V3F	probably damaging	Het
Gprin3	A	G	6: 59,330,372 (GRCm39)	L645P	probably damaging	Het
Hyal2	A	G	9: 107,449,270 (GRCm39)	Y342C	probably benign	Het
Igf2bp2	A	G	16: 21,907,849 (GRCm39)	I16T	possibly damaging	Het
Inca1	T	C	11: 70,581,025 (GRCm39)	Y61C	probably damaging	Het
Ipo5	T	C	14: 121,180,145 (GRCm39)	W860R	possibly damaging	Het
Kcnj9	C	T	1: 172,150,591 (GRCm39)	A341T	probably benign	Het
Kdm5b	T	C	1: 134,530,309 (GRCm39)	V440A	probably damaging	Het
L3mbtl1	C	T	2: 162,789,255 (GRCm39)		probably benign	Het
Lin54	G	A	5: 100,600,152 (GRCm39)	T307I	probably damaging	Het
Lrrc18	C	A	14: 32,731,096 (GRCm39)	Q212K	probably benign	Het
Lrrc37a	A	G	11: 103,393,851 (GRCm39)	S525P	probably benign	Het
Lrrc71	T	A	3: 87,653,006 (GRCm39)	S137C	probably damaging	Het
Lrrk1	A	T	7: 65,940,656 (GRCm39)		probably null	Het
Man2b2	G	A	5: 36,973,542 (GRCm39)	S58L	probably benign	Het
Masp1	T	A	16: 23,276,888 (GRCm39)	H539L	probably benign	Het
Med1	G	A	11: 98,047,730 (GRCm39)	P1022L	probably damaging	Het
Meis1	T	A	11: 18,961,360 (GRCm39)	H171L	probably damaging	Het
Mier1	T	A	4: 103,012,820 (GRCm39)		probably benign	Het
Mmp13	A	T	9: 7,272,929 (GRCm39)	R96S	probably damaging	Het
Mms19	G	A	19: 41,942,173 (GRCm39)	T38I	probably damaging	Het
Mrc1	G	A	2: 14,314,843 (GRCm39)	C976Y	probably damaging	Het
Mrtfb	C	T	16: 13,230,390 (GRCm39)	T1025I	possibly damaging	Het
Naalad2	A	G	9: 18,297,191 (GRCm39)	Y32H	probably benign	Het
Ndufs1	A	G	1: 63,183,085 (GRCm39)		probably benign	Het
Nefm	C	T	14: 68,361,608 (GRCm39)	D219N	probably damaging	Het
Nwd1	C	T	8: 73,388,965 (GRCm39)	P172L	probably damaging	Het
Nwd2	T	A	5: 63,962,454 (GRCm39)	D679E	probably damaging	Het
Ogdh	T	A	11: 6,289,936 (GRCm39)		probably benign	Het
Olfm3	T	A	3: 114,916,330 (GRCm39)	S421T	possibly damaging	Het
Opn5	T	G	17: 42,903,844 (GRCm39)	T164P	possibly damaging	Het
Or4k37	A	T	2: 111,159,673 (GRCm39)	N303I	probably benign	Het
Or51a25	T	A	7: 102,373,236 (GRCm39)	I154F	probably damaging	Het
Or52a24	T	C	7: 103,381,583 (GRCm39)	V150A	probably benign	Het
Or5ak20	A	G	2: 85,184,093 (GRCm39)	M59T	possibly damaging	Het
Or5b12b	T	C	19: 12,861,443 (GRCm39)	L66P	probably damaging	Het
Or5b12b	A	G	19: 12,861,910 (GRCm39)	T222A	probably damaging	Het
Pde7b	C	T	10: 20,314,492 (GRCm39)	V166M	probably damaging	Het
Peds1	A	T	2: 167,486,907 (GRCm39)		probably benign	Het
Pik3ap1	T	C	19: 41,313,003 (GRCm39)	N370S	probably damaging	Het
Pkhd1l1	A	T	15: 44,452,814 (GRCm39)	D3913V	probably damaging	Het
Pld1	A	G	3: 28,174,971 (GRCm39)	I90V	possibly damaging	Het
Plxna2	A	G	1: 194,326,656 (GRCm39)	T197A	possibly damaging	Het
Plxna4	A	T	6: 32,179,054 (GRCm39)	M987K	probably benign	Het
Pmch	A	G	10: 87,927,221 (GRCm39)	N75D	probably benign	Het
Prom2	T	A	2: 127,374,787 (GRCm39)	Q583L	possibly damaging	Het
Pyroxd1	T	A	6: 142,299,288 (GRCm39)	M148K	possibly damaging	Het
R3hdm2	C	T	10: 127,293,569 (GRCm39)	L158F	probably damaging	Het
Rapgef6	A	T	11: 54,516,789 (GRCm39)	T349S	probably benign	Het
Rfx5	C	T	3: 94,863,666 (GRCm39)	T105I	probably damaging	Het
Rif1	C	A	2: 52,000,749 (GRCm39)	P1401Q	probably damaging	Het
Rpn1	T	A	6: 88,067,224 (GRCm39)	S195T	probably benign	Het
Rslcan18	C	A	13: 67,250,183 (GRCm39)	K17N	probably benign	Het
Rsph3b	A	T	17: 7,209,126 (GRCm39)	I48N	probably damaging	Het
Sbf2	A	T	7: 109,998,550 (GRCm39)	Y628N	probably damaging	Het
Sis	T	C	3: 72,867,629 (GRCm39)	T139A	probably benign	Het
Slc22a14	A	G	9: 119,001,100 (GRCm39)		probably benign	Het
Slitrk6	A	T	14: 110,987,364 (GRCm39)	L781H	probably damaging	Het
Smarcb1	T	C	10: 75,732,900 (GRCm39)	T372A	probably damaging	Het
Spidr	T	A	16: 15,855,531 (GRCm39)	H328L	probably damaging	Het
Sun5	T	A	2: 153,712,872 (GRCm39)	D16V	probably damaging	Het
Syde2	G	A	3: 145,720,135 (GRCm39)	E1053K	possibly damaging	Het
Syne2	T	C	12: 76,146,238 (GRCm39)	S6419P	probably damaging	Het
Tenm3	G	A	8: 48,794,195 (GRCm39)		probably benign	Het
Timm44	C	A	8: 4,310,532 (GRCm39)	E407*	probably null	Het
Tnpo2	A	G	8: 85,773,991 (GRCm39)	T342A	probably benign	Het
Trio	A	G	15: 27,767,993 (GRCm39)	C1964R	probably benign	Het
Trip11	A	C	12: 101,851,931 (GRCm39)	L711R	probably damaging	Het
Trp53bp1	A	T	2: 121,100,450 (GRCm39)	H101Q	probably damaging	Het
Trpm6	A	G	19: 18,851,266 (GRCm39)		probably benign	Het
Ttn	A	T	2: 76,680,335 (GRCm39)		probably benign	Het
Ucp1	T	A	8: 84,021,936 (GRCm39)	M256K	possibly damaging	Het
Unc5a	T	A	13: 55,152,767 (GRCm39)	S838T	probably damaging	Het
Uxs1	T	C	1: 43,804,046 (GRCm39)		probably null	Het
Vmn2r108	A	T	17: 20,683,096 (GRCm39)	C703S	possibly damaging	Het
Zc3hav1	C	T	6: 38,309,599 (GRCm39)	G408R	probably damaging	Het
Zfp1004	T	A	2: 150,035,000 (GRCm39)	C471*	probably null	Het
Zfp609	G	A	9: 65,610,744 (GRCm39)	L740F	possibly damaging	Het
Zfp69	T	C	4: 120,788,292 (GRCm39)	E341G	probably damaging	Het
Zfp707	A	T	15: 75,847,105 (GRCm39)	H312L	probably damaging	Het
Zfp773	T	C	7: 7,136,023 (GRCm39)	D191G	probably benign	Het
Zgrf1	C	A	3: 127,366,887 (GRCm39)	D755E	probably benign	Het
Zscan5b	T	A	7: 6,242,074 (GRCm39)	I431N	probably damaging	Het

Other mutations in Robo3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00927:Robo3	APN	9	37,339,050 (GRCm39)	critical splice donor site	probably null
IGL01023:Robo3	APN	9	37,340,847 (GRCm39)	missense	probably damaging	1.00
IGL01431:Robo3	APN	9	37,330,407 (GRCm39)	unclassified	probably benign
IGL01993:Robo3	APN	9	37,335,949 (GRCm39)	missense	probably damaging	1.00
IGL02256:Robo3	APN	9	37,336,649 (GRCm39)	missense	probably damaging	1.00
IGL02323:Robo3	APN	9	37,333,497 (GRCm39)	missense	probably benign	0.05
IGL02561:Robo3	APN	9	37,338,387 (GRCm39)	missense	possibly damaging	0.84
IGL02866:Robo3	APN	9	37,333,602 (GRCm39)	missense	possibly damaging	0.89
IGL02897:Robo3	APN	9	37,338,798 (GRCm39)	nonsense	probably null
IGL03003:Robo3	APN	9	37,330,587 (GRCm39)	missense	probably damaging	1.00
IGL03307:Robo3	APN	9	37,333,860 (GRCm39)	missense	probably damaging	0.96
IGL03097:Robo3	UTSW	9	37,333,824 (GRCm39)	critical splice donor site	probably null
R0137:Robo3	UTSW	9	37,336,640 (GRCm39)	missense	probably benign	0.00
R0266:Robo3	UTSW	9	37,333,936 (GRCm39)	missense	probably damaging	0.96
R0390:Robo3	UTSW	9	37,333,473 (GRCm39)	missense	probably benign	0.00
R0815:Robo3	UTSW	9	37,333,479 (GRCm39)	missense	probably damaging	1.00
R0924:Robo3	UTSW	9	37,340,778 (GRCm39)	splice site	probably benign
R1167:Robo3	UTSW	9	37,335,203 (GRCm39)	nonsense	probably null
R1203:Robo3	UTSW	9	37,329,978 (GRCm39)	missense	probably damaging	1.00
R1451:Robo3	UTSW	9	37,329,007 (GRCm39)	missense	probably benign	0.01
R1575:Robo3	UTSW	9	37,340,957 (GRCm39)	missense	probably damaging	1.00
R1596:Robo3	UTSW	9	37,335,928 (GRCm39)	critical splice donor site	probably null
R1660:Robo3	UTSW	9	37,340,440 (GRCm39)	missense	probably damaging	1.00
R1677:Robo3	UTSW	9	37,329,005 (GRCm39)	missense	possibly damaging	0.75
R1839:Robo3	UTSW	9	37,333,623 (GRCm39)	missense	probably benign	0.00
R1878:Robo3	UTSW	9	37,333,461 (GRCm39)	missense	probably damaging	1.00
R1891:Robo3	UTSW	9	37,339,351 (GRCm39)	missense	probably damaging	1.00
R2040:Robo3	UTSW	9	37,338,760 (GRCm39)	missense	probably damaging	1.00
R2859:Robo3	UTSW	9	37,339,400 (GRCm39)	nonsense	probably null
R3786:Robo3	UTSW	9	37,333,521 (GRCm39)	missense	probably damaging	1.00
R3886:Robo3	UTSW	9	37,333,477 (GRCm39)	nonsense	probably null
R3888:Robo3	UTSW	9	37,333,477 (GRCm39)	nonsense	probably null
R3910:Robo3	UTSW	9	37,330,591 (GRCm39)	missense	probably damaging	1.00
R4212:Robo3	UTSW	9	37,333,194 (GRCm39)	missense	probably damaging	1.00
R4213:Robo3	UTSW	9	37,333,194 (GRCm39)	missense	probably damaging	1.00
R4691:Robo3	UTSW	9	37,336,514 (GRCm39)	missense	probably damaging	0.99
R4979:Robo3	UTSW	9	37,334,640 (GRCm39)	missense	probably damaging	1.00
R5238:Robo3	UTSW	9	37,328,175 (GRCm39)	missense	probably damaging	0.99
R5570:Robo3	UTSW	9	37,336,571 (GRCm39)	missense	possibly damaging	0.81
R5629:Robo3	UTSW	9	37,330,507 (GRCm39)	nonsense	probably null
R5770:Robo3	UTSW	9	37,330,497 (GRCm39)	missense	possibly damaging	0.87
R5837:Robo3	UTSW	9	37,341,112 (GRCm39)	critical splice acceptor site	probably null
R6021:Robo3	UTSW	9	37,333,829 (GRCm39)	nonsense	probably null
R6129:Robo3	UTSW	9	37,334,589 (GRCm39)	missense	probably benign
R6232:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6233:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6235:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6326:Robo3	UTSW	9	37,338,323 (GRCm39)	missense	probably damaging	1.00
R6354:Robo3	UTSW	9	37,328,513 (GRCm39)	unclassified	probably benign
R6355:Robo3	UTSW	9	37,330,235 (GRCm39)	missense	possibly damaging	0.71
R6475:Robo3	UTSW	9	37,334,586 (GRCm39)	missense	probably damaging	0.99
R6937:Robo3	UTSW	9	37,341,176 (GRCm39)	missense	probably benign	0.16
R7201:Robo3	UTSW	9	37,335,626 (GRCm39)	nonsense	probably null
R7208:Robo3	UTSW	9	37,336,020 (GRCm39)	missense	probably damaging	0.99
R7249:Robo3	UTSW	9	37,336,129 (GRCm39)	missense	probably benign
R7376:Robo3	UTSW	9	37,344,212 (GRCm39)	missense	probably damaging	1.00
R7380:Robo3	UTSW	9	37,329,852 (GRCm39)	missense	probably damaging	1.00
R7448:Robo3	UTSW	9	37,336,111 (GRCm39)	missense	possibly damaging	0.89
R7475:Robo3	UTSW	9	37,336,674 (GRCm39)	missense	probably benign	0.01
R7496:Robo3	UTSW	9	37,339,121 (GRCm39)	missense	probably damaging	1.00
R7587:Robo3	UTSW	9	37,340,942 (GRCm39)	missense	probably damaging	1.00
R7694:Robo3	UTSW	9	37,329,816 (GRCm39)	missense	probably benign	0.14
R8381:Robo3	UTSW	9	37,341,056 (GRCm39)	missense	probably damaging	1.00
R8464:Robo3	UTSW	9	37,332,726 (GRCm39)	missense	probably damaging	1.00
R8495:Robo3	UTSW	9	37,336,664 (GRCm39)	missense	probably damaging	1.00
R8886:Robo3	UTSW	9	37,328,768 (GRCm39)	missense	probably damaging	0.99
R9422:Robo3	UTSW	9	37,329,789 (GRCm39)	missense	probably benign	0.03
R9563:Robo3	UTSW	9	37,340,900 (GRCm39)	missense	probably damaging	1.00
R9564:Robo3	UTSW	9	37,340,900 (GRCm39)	missense	probably damaging	1.00
R9681:Robo3	UTSW	9	37,339,087 (GRCm39)	missense	probably benign	0.45
R9681:Robo3	UTSW	9	37,334,558 (GRCm39)	missense	possibly damaging	0.75
X0024:Robo3	UTSW	9	37,339,151 (GRCm39)	missense	probably damaging	1.00
X0027:Robo3	UTSW	9	37,339,121 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGTCACAGGCCATGCATAGGAAG -3'
(R):5'- GTTTACAGGGTGCCAGTGGACAAG -3'

Sequencing Primer
(F):5'- TACACCTCCTATGGCATAGTCAG -3'
(R):5'- ACAAGTGGTGACAGTCTGCTTC -3'

Posted On

2013-06-12