Incidental Mutation 'R0506:Tatdn2'
ID 47577
Institutional Source Beutler Lab
Gene Symbol Tatdn2
Ensembl Gene ENSMUSG00000056952
Gene Name TatD DNase domain containing 2
Synonyms mKIAA0218
MMRRC Submission 038701-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0506 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 113674090-113688030 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to A at 113679550 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 298 (D298E)
Ref Sequence ENSEMBL: ENSMUSP00000145308 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000089018] [ENSMUST00000113022] [ENSMUST00000153661] [ENSMUST00000204753]
AlphaFold B7ZNL9
Predicted Effect probably benign
Transcript: ENSMUST00000089018
SMART Domains Protein: ENSMUSP00000086412
Gene: ENSMUSG00000056952

DomainStartEndE-ValueType
low complexity region 63 95 N/A INTRINSIC
low complexity region 237 255 N/A INTRINSIC
low complexity region 382 392 N/A INTRINSIC
Pfam:TatD_DNase 457 721 3.3e-59 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000113022
AA Change: D298E

PolyPhen 2 Score 0.131 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000108645
Gene: ENSMUSG00000056952
AA Change: D298E

DomainStartEndE-ValueType
low complexity region 63 95 N/A INTRINSIC
low complexity region 237 255 N/A INTRINSIC
low complexity region 443 453 N/A INTRINSIC
Pfam:TatD_DNase 518 782 3.7e-61 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000138420
SMART Domains Protein: ENSMUSP00000116559
Gene: ENSMUSG00000056952

DomainStartEndE-ValueType
Pfam:TatD_DNase 1 146 2.9e-38 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000153661
SMART Domains Protein: ENSMUSP00000123557
Gene: ENSMUSG00000056952

DomainStartEndE-ValueType
Pfam:TatD_DNase 1 122 1.3e-22 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000200187
Predicted Effect probably benign
Transcript: ENSMUST00000204753
AA Change: D298E

PolyPhen 2 Score 0.131 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000145308
Gene: ENSMUSG00000056952
AA Change: D298E

DomainStartEndE-ValueType
low complexity region 63 95 N/A INTRINSIC
low complexity region 237 255 N/A INTRINSIC
low complexity region 443 453 N/A INTRINSIC
Pfam:TatD_DNase 518 782 3.7e-61 PFAM
Meta Mutation Damage Score 0.0606 question?
Coding Region Coverage
  • 1x: 99.6%
  • 3x: 98.8%
  • 10x: 96.7%
  • 20x: 93.3%
Validation Efficiency 100% (100/100)
Allele List at MGI
Other mutations in this stock
Total: 97 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsm5 T A 7: 119,137,319 (GRCm39) C378* probably null Het
Ago3 T C 4: 126,311,045 (GRCm39) D56G possibly damaging Het
Ahnak G T 19: 8,986,492 (GRCm39) G2592V probably damaging Het
Aldh6a1 C T 12: 84,480,300 (GRCm39) G470D probably damaging Het
Ankub1 T A 3: 57,597,796 (GRCm39) N58I probably damaging Het
Apol7b G T 15: 77,309,728 (GRCm39) T23K probably benign Het
Arap2 G A 5: 62,763,474 (GRCm39) P1557S possibly damaging Het
Arhgap24 T C 5: 103,023,643 (GRCm39) Y136H probably damaging Het
Atp1a1 A G 3: 101,497,128 (GRCm39) F393L probably damaging Het
Bcdin3d A T 15: 99,368,873 (GRCm39) C109S probably damaging Het
Catsperd A G 17: 56,965,078 (GRCm39) K475R possibly damaging Het
Cblb A G 16: 52,024,843 (GRCm39) T913A probably benign Het
Cbx6 A G 15: 79,712,404 (GRCm39) L341P probably benign Het
Cd177 T C 7: 24,457,781 (GRCm39) Y159C probably damaging Het
Cdh20 A G 1: 110,027,844 (GRCm39) N530D probably damaging Het
Cdk8 T C 5: 146,235,682 (GRCm39) F270L probably damaging Het
Ces2c A T 8: 105,574,656 (GRCm39) T38S probably damaging Het
Chst14 T C 2: 118,758,202 (GRCm39) L357P probably damaging Het
Clca3b T A 3: 144,528,627 (GRCm39) probably benign Het
Cluh A G 11: 74,555,720 (GRCm39) S839G probably benign Het
Cnga4 T A 7: 105,056,947 (GRCm39) V350E probably damaging Het
Creb1 G A 1: 64,609,426 (GRCm39) G180R probably damaging Het
Csmd3 T C 15: 48,320,907 (GRCm39) E301G probably benign Het
Cyp4f18 A T 8: 72,749,844 (GRCm39) D268E probably benign Het
Dock5 A G 14: 68,022,241 (GRCm39) probably benign Het
Dpy19l4 T A 4: 11,289,715 (GRCm39) H332L probably benign Het
Dync2h1 T A 9: 7,113,153 (GRCm39) H224L probably benign Het
Dzip1l C A 9: 99,545,134 (GRCm39) Q585K possibly damaging Het
Erf C T 7: 24,943,801 (GRCm39) G510D probably damaging Het
Fanci T C 7: 79,081,926 (GRCm39) L623P probably benign Het
Fat1 T C 8: 45,475,988 (GRCm39) V1655A probably damaging Het
Fat4 T C 3: 38,942,463 (GRCm39) V452A probably benign Het
Gal3st4 C T 5: 138,264,151 (GRCm39) G283S probably benign Het
Gm5422 A G 10: 31,126,318 (GRCm39) noncoding transcript Het
Gnal C T 18: 67,221,744 (GRCm39) T49I unknown Het
Gng5 A G 3: 146,209,103 (GRCm39) N57S probably damaging Het
Herc1 A G 9: 66,355,441 (GRCm39) I2231V probably damaging Het
Hgfac G T 5: 35,201,584 (GRCm39) G272W probably damaging Het
Hmcn1 T A 1: 150,618,092 (GRCm39) D1265V possibly damaging Het
Ifi207 T A 1: 173,563,878 (GRCm39) Q47L possibly damaging Het
Klhl40 G A 9: 121,607,133 (GRCm39) E98K probably damaging Het
Lepr G T 4: 101,630,207 (GRCm39) probably benign Het
Lyst A G 13: 13,812,600 (GRCm39) H1004R probably benign Het
Map3k1 T A 13: 111,892,298 (GRCm39) R986* probably null Het
Mmp1b C A 9: 7,387,013 (GRCm39) Q66H possibly damaging Het
Mpo T C 11: 87,694,330 (GRCm39) S107P probably benign Het
Mroh9 T C 1: 162,888,205 (GRCm39) H290R possibly damaging Het
Myo7b A G 18: 32,097,439 (GRCm39) probably null Het
Myom1 T C 17: 71,399,215 (GRCm39) probably benign Het
Nalcn C T 14: 123,834,026 (GRCm39) V50I possibly damaging Het
Negr1 A G 3: 156,866,385 (GRCm39) probably benign Het
Nlrc5 T G 8: 95,219,753 (GRCm39) probably benign Het
Nyap2 G A 1: 81,065,029 (GRCm39) D14N probably damaging Het
Or10w1 T A 19: 13,632,261 (GRCm39) I151N possibly damaging Het
Or2h1 C A 17: 37,404,203 (GRCm39) G188W probably damaging Het
Or5b105 G A 19: 13,080,642 (GRCm39) R3C possibly damaging Het
Parp14 A T 16: 35,661,779 (GRCm39) S1419T possibly damaging Het
Piezo2 A G 18: 63,160,615 (GRCm39) F2347S probably damaging Het
Pigf A G 17: 87,316,337 (GRCm39) V147A probably benign Het
Pkhd1 A T 1: 20,629,693 (GRCm39) M637K probably benign Het
Plce1 T C 19: 38,748,582 (GRCm39) I1771T probably benign Het
Ppp6c A T 2: 39,096,660 (GRCm39) probably benign Het
Prag1 T C 8: 36,570,854 (GRCm39) V479A possibly damaging Het
Prss33 A T 17: 24,054,079 (GRCm39) D42E probably benign Het
Psmb10 A G 8: 106,664,177 (GRCm39) V64A possibly damaging Het
Psmd14 A G 2: 61,630,407 (GRCm39) T306A probably benign Het
Psmg1 C T 16: 95,790,687 (GRCm39) probably benign Het
Rc3h2 A T 2: 37,266,671 (GRCm39) probably null Het
Reln C T 5: 22,125,494 (GRCm39) V2730I probably damaging Het
Sec24a A T 11: 51,634,622 (GRCm39) H101Q probably benign Het
Selenoi A G 5: 30,471,954 (GRCm39) N385S probably benign Het
Slc24a4 T C 12: 102,097,882 (GRCm39) probably null Het
Slc4a10 G A 2: 62,080,877 (GRCm39) S338N probably benign Het
Slfn3 A T 11: 83,103,986 (GRCm39) T286S probably damaging Het
Snx29 A G 16: 11,213,167 (GRCm39) D111G probably benign Het
Sp8 T C 12: 118,812,300 (GRCm39) S52P possibly damaging Het
Srek1 G T 13: 103,897,098 (GRCm39) T81K probably damaging Het
Sry C G Y: 2,662,864 (GRCm39) Q265H unknown Het
Taf3 A G 2: 9,945,804 (GRCm39) V600A probably benign Het
Tmem253 A T 14: 52,254,663 (GRCm39) probably benign Het
Tmem63a T A 1: 180,785,614 (GRCm39) probably null Het
Tmprss11b T C 5: 86,809,499 (GRCm39) D331G probably damaging Het
Tor1aip1 T A 1: 155,883,420 (GRCm39) K143* probably null Het
Trappc8 A T 18: 20,977,245 (GRCm39) N841K possibly damaging Het
Trio T C 15: 27,855,049 (GRCm39) Q711R probably benign Het
Trmt10b C A 4: 45,304,306 (GRCm39) T114N probably damaging Het
Trpv2 C A 11: 62,473,732 (GRCm39) A129D probably benign Het
Ttll4 T G 1: 74,727,777 (GRCm39) D846E probably benign Het
Ugt2a3 A G 5: 87,484,508 (GRCm39) L172P possibly damaging Het
Usp19 T A 9: 108,371,686 (GRCm39) F355Y probably damaging Het
Vmn1r209 C T 13: 22,990,114 (GRCm39) G192D probably damaging Het
Vmn2r107 T G 17: 20,578,021 (GRCm39) D443E probably benign Het
Wee2 A T 6: 40,440,187 (GRCm39) E445V probably benign Het
Zer1 A T 2: 29,991,819 (GRCm39) I680N probably damaging Het
Zfhx4 T C 3: 5,467,795 (GRCm39) L2651P probably damaging Het
Zfp692 C T 11: 58,199,881 (GRCm39) Q157* probably null Het
Zfp964 T A 8: 70,116,587 (GRCm39) C396S unknown Het
Other mutations in Tatdn2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01302:Tatdn2 APN 6 113,680,985 (GRCm39) splice site probably benign
IGL01335:Tatdn2 APN 6 113,681,017 (GRCm39) missense probably benign 0.29
IGL01459:Tatdn2 APN 6 113,686,992 (GRCm39) splice site probably null
IGL02406:Tatdn2 APN 6 113,681,174 (GRCm39) missense probably benign 0.41
IGL02728:Tatdn2 APN 6 113,681,676 (GRCm39) missense probably damaging 1.00
R0321:Tatdn2 UTSW 6 113,686,462 (GRCm39) missense probably damaging 1.00
R0583:Tatdn2 UTSW 6 113,679,486 (GRCm39) missense possibly damaging 0.80
R1022:Tatdn2 UTSW 6 113,686,506 (GRCm39) missense probably damaging 1.00
R1024:Tatdn2 UTSW 6 113,686,506 (GRCm39) missense probably damaging 1.00
R1301:Tatdn2 UTSW 6 113,681,076 (GRCm39) missense probably damaging 1.00
R1454:Tatdn2 UTSW 6 113,681,288 (GRCm39) missense probably benign 0.26
R1459:Tatdn2 UTSW 6 113,687,031 (GRCm39) missense probably damaging 1.00
R1710:Tatdn2 UTSW 6 113,674,888 (GRCm39) missense possibly damaging 0.90
R1771:Tatdn2 UTSW 6 113,679,060 (GRCm39) critical splice acceptor site probably null
R2064:Tatdn2 UTSW 6 113,681,103 (GRCm39) missense probably benign 0.41
R2065:Tatdn2 UTSW 6 113,681,103 (GRCm39) missense probably benign 0.41
R2067:Tatdn2 UTSW 6 113,681,103 (GRCm39) missense probably benign 0.41
R4446:Tatdn2 UTSW 6 113,679,501 (GRCm39) critical splice donor site probably null
R4654:Tatdn2 UTSW 6 113,684,326 (GRCm39) missense probably benign 0.09
R4888:Tatdn2 UTSW 6 113,681,566 (GRCm39) missense possibly damaging 0.66
R7378:Tatdn2 UTSW 6 113,681,662 (GRCm39) missense probably damaging 1.00
R7971:Tatdn2 UTSW 6 113,687,235 (GRCm39) splice site probably null
R8086:Tatdn2 UTSW 6 113,686,482 (GRCm39) missense probably damaging 1.00
R8833:Tatdn2 UTSW 6 113,684,348 (GRCm39) missense probably damaging 1.00
R8872:Tatdn2 UTSW 6 113,681,170 (GRCm39) missense probably damaging 1.00
R9186:Tatdn2 UTSW 6 113,687,125 (GRCm39) missense probably benign 0.13
R9234:Tatdn2 UTSW 6 113,679,683 (GRCm39) critical splice donor site probably null
Predicted Primers PCR Primer
(F):5'- GAGTGATGCTATGGAGGAACCCAAC -3'
(R):5'- TTCCTAAGGCCCTGAGTCTGATCC -3'

Sequencing Primer
(F):5'- AAGCTTGGGAGACTGTCCTAATC -3'
(R):5'- CTGATCCTCCTAATGGTGGGAAAC -3'
Posted On 2013-06-12