Incidental Mutation 'R0506:Zfp964'
ID 47585
Institutional Source Beutler Lab
Gene Symbol Zfp964
Ensembl Gene ENSMUSG00000091764
Gene Name zinc finger protein 964
Synonyms Gm7187
MMRRC Submission 038701-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.103) question?
Stock # R0506 (G1)
Quality Score 225
Status Validated
Chromosome 8
Chromosomal Location 70107129-70119632 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 70116587 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Serine at position 396 (C396S)
Ref Sequence ENSEMBL: ENSMUSP00000145354 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000169125] [ENSMUST00000204285]
AlphaFold B2RR88
Predicted Effect unknown
Transcript: ENSMUST00000169125
AA Change: C395S
SMART Domains Protein: ENSMUSP00000129822
Gene: ENSMUSG00000091764
AA Change: C395S

DomainStartEndE-ValueType
KRAB 3 56 5.24e-18 SMART
ZnF_C2H2 216 238 4.54e-4 SMART
ZnF_C2H2 244 266 3.58e-2 SMART
ZnF_C2H2 272 294 2.2e-2 SMART
ZnF_C2H2 300 322 5.5e-3 SMART
ZnF_C2H2 328 350 8.22e-2 SMART
ZnF_C2H2 356 378 2.05e-2 SMART
ZnF_C2H2 384 406 6.32e-3 SMART
ZnF_C2H2 412 434 5.42e-2 SMART
ZnF_C2H2 440 462 1.28e-3 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204062
Predicted Effect unknown
Transcript: ENSMUST00000204285
AA Change: C396S
SMART Domains Protein: ENSMUSP00000145354
Gene: ENSMUSG00000091764
AA Change: C396S

DomainStartEndE-ValueType
KRAB 4 57 5.24e-18 SMART
ZnF_C2H2 217 239 4.54e-4 SMART
ZnF_C2H2 245 267 3.58e-2 SMART
ZnF_C2H2 273 295 2.2e-2 SMART
ZnF_C2H2 301 323 5.5e-3 SMART
ZnF_C2H2 329 351 8.22e-2 SMART
ZnF_C2H2 357 379 2.05e-2 SMART
ZnF_C2H2 385 407 6.32e-3 SMART
ZnF_C2H2 413 435 5.42e-2 SMART
ZnF_C2H2 441 463 1.28e-3 SMART
Meta Mutation Damage Score 0.0869 question?
Coding Region Coverage
  • 1x: 99.6%
  • 3x: 98.8%
  • 10x: 96.7%
  • 20x: 93.3%
Validation Efficiency 100% (100/100)
Allele List at MGI
Other mutations in this stock
Total: 97 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsm5 T A 7: 119,137,319 (GRCm39) C378* probably null Het
Ago3 T C 4: 126,311,045 (GRCm39) D56G possibly damaging Het
Ahnak G T 19: 8,986,492 (GRCm39) G2592V probably damaging Het
Aldh6a1 C T 12: 84,480,300 (GRCm39) G470D probably damaging Het
Ankub1 T A 3: 57,597,796 (GRCm39) N58I probably damaging Het
Apol7b G T 15: 77,309,728 (GRCm39) T23K probably benign Het
Arap2 G A 5: 62,763,474 (GRCm39) P1557S possibly damaging Het
Arhgap24 T C 5: 103,023,643 (GRCm39) Y136H probably damaging Het
Atp1a1 A G 3: 101,497,128 (GRCm39) F393L probably damaging Het
Bcdin3d A T 15: 99,368,873 (GRCm39) C109S probably damaging Het
Catsperd A G 17: 56,965,078 (GRCm39) K475R possibly damaging Het
Cblb A G 16: 52,024,843 (GRCm39) T913A probably benign Het
Cbx6 A G 15: 79,712,404 (GRCm39) L341P probably benign Het
Cd177 T C 7: 24,457,781 (GRCm39) Y159C probably damaging Het
Cdh20 A G 1: 110,027,844 (GRCm39) N530D probably damaging Het
Cdk8 T C 5: 146,235,682 (GRCm39) F270L probably damaging Het
Ces2c A T 8: 105,574,656 (GRCm39) T38S probably damaging Het
Chst14 T C 2: 118,758,202 (GRCm39) L357P probably damaging Het
Clca3b T A 3: 144,528,627 (GRCm39) probably benign Het
Cluh A G 11: 74,555,720 (GRCm39) S839G probably benign Het
Cnga4 T A 7: 105,056,947 (GRCm39) V350E probably damaging Het
Creb1 G A 1: 64,609,426 (GRCm39) G180R probably damaging Het
Csmd3 T C 15: 48,320,907 (GRCm39) E301G probably benign Het
Cyp4f18 A T 8: 72,749,844 (GRCm39) D268E probably benign Het
Dock5 A G 14: 68,022,241 (GRCm39) probably benign Het
Dpy19l4 T A 4: 11,289,715 (GRCm39) H332L probably benign Het
Dync2h1 T A 9: 7,113,153 (GRCm39) H224L probably benign Het
Dzip1l C A 9: 99,545,134 (GRCm39) Q585K possibly damaging Het
Erf C T 7: 24,943,801 (GRCm39) G510D probably damaging Het
Fanci T C 7: 79,081,926 (GRCm39) L623P probably benign Het
Fat1 T C 8: 45,475,988 (GRCm39) V1655A probably damaging Het
Fat4 T C 3: 38,942,463 (GRCm39) V452A probably benign Het
Gal3st4 C T 5: 138,264,151 (GRCm39) G283S probably benign Het
Gm5422 A G 10: 31,126,318 (GRCm39) noncoding transcript Het
Gnal C T 18: 67,221,744 (GRCm39) T49I unknown Het
Gng5 A G 3: 146,209,103 (GRCm39) N57S probably damaging Het
Herc1 A G 9: 66,355,441 (GRCm39) I2231V probably damaging Het
Hgfac G T 5: 35,201,584 (GRCm39) G272W probably damaging Het
Hmcn1 T A 1: 150,618,092 (GRCm39) D1265V possibly damaging Het
Ifi207 T A 1: 173,563,878 (GRCm39) Q47L possibly damaging Het
Klhl40 G A 9: 121,607,133 (GRCm39) E98K probably damaging Het
Lepr G T 4: 101,630,207 (GRCm39) probably benign Het
Lyst A G 13: 13,812,600 (GRCm39) H1004R probably benign Het
Map3k1 T A 13: 111,892,298 (GRCm39) R986* probably null Het
Mmp1b C A 9: 7,387,013 (GRCm39) Q66H possibly damaging Het
Mpo T C 11: 87,694,330 (GRCm39) S107P probably benign Het
Mroh9 T C 1: 162,888,205 (GRCm39) H290R possibly damaging Het
Myo7b A G 18: 32,097,439 (GRCm39) probably null Het
Myom1 T C 17: 71,399,215 (GRCm39) probably benign Het
Nalcn C T 14: 123,834,026 (GRCm39) V50I possibly damaging Het
Negr1 A G 3: 156,866,385 (GRCm39) probably benign Het
Nlrc5 T G 8: 95,219,753 (GRCm39) probably benign Het
Nyap2 G A 1: 81,065,029 (GRCm39) D14N probably damaging Het
Or10w1 T A 19: 13,632,261 (GRCm39) I151N possibly damaging Het
Or2h1 C A 17: 37,404,203 (GRCm39) G188W probably damaging Het
Or5b105 G A 19: 13,080,642 (GRCm39) R3C possibly damaging Het
Parp14 A T 16: 35,661,779 (GRCm39) S1419T possibly damaging Het
Piezo2 A G 18: 63,160,615 (GRCm39) F2347S probably damaging Het
Pigf A G 17: 87,316,337 (GRCm39) V147A probably benign Het
Pkhd1 A T 1: 20,629,693 (GRCm39) M637K probably benign Het
Plce1 T C 19: 38,748,582 (GRCm39) I1771T probably benign Het
Ppp6c A T 2: 39,096,660 (GRCm39) probably benign Het
Prag1 T C 8: 36,570,854 (GRCm39) V479A possibly damaging Het
Prss33 A T 17: 24,054,079 (GRCm39) D42E probably benign Het
Psmb10 A G 8: 106,664,177 (GRCm39) V64A possibly damaging Het
Psmd14 A G 2: 61,630,407 (GRCm39) T306A probably benign Het
Psmg1 C T 16: 95,790,687 (GRCm39) probably benign Het
Rc3h2 A T 2: 37,266,671 (GRCm39) probably null Het
Reln C T 5: 22,125,494 (GRCm39) V2730I probably damaging Het
Sec24a A T 11: 51,634,622 (GRCm39) H101Q probably benign Het
Selenoi A G 5: 30,471,954 (GRCm39) N385S probably benign Het
Slc24a4 T C 12: 102,097,882 (GRCm39) probably null Het
Slc4a10 G A 2: 62,080,877 (GRCm39) S338N probably benign Het
Slfn3 A T 11: 83,103,986 (GRCm39) T286S probably damaging Het
Snx29 A G 16: 11,213,167 (GRCm39) D111G probably benign Het
Sp8 T C 12: 118,812,300 (GRCm39) S52P possibly damaging Het
Srek1 G T 13: 103,897,098 (GRCm39) T81K probably damaging Het
Sry C G Y: 2,662,864 (GRCm39) Q265H unknown Het
Taf3 A G 2: 9,945,804 (GRCm39) V600A probably benign Het
Tatdn2 C A 6: 113,679,550 (GRCm39) D298E probably benign Het
Tmem253 A T 14: 52,254,663 (GRCm39) probably benign Het
Tmem63a T A 1: 180,785,614 (GRCm39) probably null Het
Tmprss11b T C 5: 86,809,499 (GRCm39) D331G probably damaging Het
Tor1aip1 T A 1: 155,883,420 (GRCm39) K143* probably null Het
Trappc8 A T 18: 20,977,245 (GRCm39) N841K possibly damaging Het
Trio T C 15: 27,855,049 (GRCm39) Q711R probably benign Het
Trmt10b C A 4: 45,304,306 (GRCm39) T114N probably damaging Het
Trpv2 C A 11: 62,473,732 (GRCm39) A129D probably benign Het
Ttll4 T G 1: 74,727,777 (GRCm39) D846E probably benign Het
Ugt2a3 A G 5: 87,484,508 (GRCm39) L172P possibly damaging Het
Usp19 T A 9: 108,371,686 (GRCm39) F355Y probably damaging Het
Vmn1r209 C T 13: 22,990,114 (GRCm39) G192D probably damaging Het
Vmn2r107 T G 17: 20,578,021 (GRCm39) D443E probably benign Het
Wee2 A T 6: 40,440,187 (GRCm39) E445V probably benign Het
Zer1 A T 2: 29,991,819 (GRCm39) I680N probably damaging Het
Zfhx4 T C 3: 5,467,795 (GRCm39) L2651P probably damaging Het
Zfp692 C T 11: 58,199,881 (GRCm39) Q157* probably null Het
Other mutations in Zfp964
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00580:Zfp964 APN 8 70,112,043 (GRCm39) splice site probably null
R0740:Zfp964 UTSW 8 70,115,828 (GRCm39) missense probably damaging 0.98
R0786:Zfp964 UTSW 8 70,116,731 (GRCm39) missense possibly damaging 0.71
R1158:Zfp964 UTSW 8 70,116,503 (GRCm39) missense unknown
R1204:Zfp964 UTSW 8 70,116,668 (GRCm39) missense probably benign 0.08
R1413:Zfp964 UTSW 8 70,115,720 (GRCm39) missense unknown
R1562:Zfp964 UTSW 8 70,115,654 (GRCm39) missense probably benign
R1663:Zfp964 UTSW 8 70,116,733 (GRCm39) splice site probably null
R1693:Zfp964 UTSW 8 70,116,800 (GRCm39) missense possibly damaging 0.55
R2029:Zfp964 UTSW 8 70,116,567 (GRCm39) missense unknown
R2847:Zfp964 UTSW 8 70,116,504 (GRCm39) missense unknown
R2849:Zfp964 UTSW 8 70,116,504 (GRCm39) missense unknown
R4111:Zfp964 UTSW 8 70,116,754 (GRCm39) missense probably benign 0.18
R4792:Zfp964 UTSW 8 70,116,665 (GRCm39) missense probably benign 0.18
R4907:Zfp964 UTSW 8 70,115,972 (GRCm39) missense possibly damaging 0.86
R4938:Zfp964 UTSW 8 70,116,758 (GRCm39) missense possibly damaging 0.64
R5688:Zfp964 UTSW 8 70,116,766 (GRCm39) missense probably benign 0.03
R5905:Zfp964 UTSW 8 70,116,563 (GRCm39) missense unknown
R6009:Zfp964 UTSW 8 70,116,106 (GRCm39) missense possibly damaging 0.71
R6021:Zfp964 UTSW 8 70,115,742 (GRCm39) missense unknown
R6028:Zfp964 UTSW 8 70,116,563 (GRCm39) missense unknown
R6374:Zfp964 UTSW 8 70,111,994 (GRCm39) missense possibly damaging 0.93
R6583:Zfp964 UTSW 8 70,115,633 (GRCm39) missense probably damaging 0.98
R7730:Zfp964 UTSW 8 70,116,360 (GRCm39) missense possibly damaging 0.85
R8263:Zfp964 UTSW 8 70,116,345 (GRCm39) missense possibly damaging 0.92
R8309:Zfp964 UTSW 8 70,115,924 (GRCm39) missense possibly damaging 0.53
R8889:Zfp964 UTSW 8 70,116,405 (GRCm39) missense probably damaging 1.00
R8892:Zfp964 UTSW 8 70,116,405 (GRCm39) missense probably damaging 1.00
R9133:Zfp964 UTSW 8 70,115,783 (GRCm39) missense probably benign 0.00
R9185:Zfp964 UTSW 8 70,115,873 (GRCm39) missense possibly damaging 0.53
Predicted Primers PCR Primer
(F):5'- AGCGGTACATGAGAACTCACGTTG -3'
(R):5'- ctggactGAATCATTTTCTGGGGCA -3'

Sequencing Primer
(F):5'- gaaagaatccatactgagaaaaaacc -3'
(R):5'- agatgactgttacgactgaagg -3'
Posted On 2013-06-12