Incidental Mutation 'R3701:Zfp446'
| ID |
475886 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp446
|
| Ensembl Gene |
ENSMUSG00000033961 |
| Gene Name |
zinc finger protein 446 |
| Synonyms |
A630035I11Rik |
| MMRRC Submission |
040694-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.080)
|
| Stock # |
R3701 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
12711726-12718323 bp(+) (GRCm39) |
| Type of Mutation |
unclassified |
| DNA Base Change (assembly) |
G to A
at 12712079 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000124620
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000038701]
[ENSMUST00000045810]
[ENSMUST00000108535]
[ENSMUST00000108536]
[ENSMUST00000108537]
[ENSMUST00000124387]
[ENSMUST00000128293]
|
| AlphaFold |
Q8C9M8 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000038701
|
| SMART Domains |
Protein: ENSMUSP00000041944
Gene: ENSMUSG00000004500
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
31 |
91 |
8.89e-30 |
SMART |
|
ZnF_C2H2
|
291 |
313 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
319 |
341 |
1.82e-3 |
SMART |
|
ZnF_C2H2
|
347 |
369 |
6.88e-4 |
SMART |
|
ZnF_C2H2
|
375 |
397 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
403 |
425 |
2.71e-2 |
SMART |
|
ZnF_C2H2
|
431 |
453 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
459 |
481 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
487 |
509 |
1.25e-1 |
SMART |
|
ZnF_C2H2
|
515 |
537 |
7.37e-4 |
SMART |
|
low complexity region
|
564 |
579 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000045810
|
| SMART Domains |
Protein: ENSMUSP00000039073
Gene: ENSMUSG00000033961
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
67 |
79 |
N/A |
INTRINSIC |
|
SCAN
|
122 |
234 |
1.29e-53 |
SMART |
|
KRAB
|
299 |
360 |
3.96e-2 |
SMART |
|
ZnF_C2H2
|
419 |
441 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
468 |
490 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
496 |
518 |
5.34e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108535
|
| SMART Domains |
Protein: ENSMUSP00000104175
Gene: ENSMUSG00000033961
| Domain | Start | End | E-Value | Type |
|---|
|
SCAN
|
22 |
134 |
1.29e-53 |
SMART |
|
KRAB
|
199 |
254 |
1.14e0 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108536
|
| SMART Domains |
Protein: ENSMUSP00000104176
Gene: ENSMUSG00000033961
| Domain | Start | End | E-Value | Type |
|---|
|
SCAN
|
22 |
134 |
1.29e-53 |
SMART |
|
KRAB
|
199 |
260 |
3.96e-2 |
SMART |
|
ZnF_C2H2
|
319 |
341 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
368 |
390 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
396 |
418 |
5.34e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108537
|
| SMART Domains |
Protein: ENSMUSP00000104177
Gene: ENSMUSG00000033961
| Domain | Start | End | E-Value | Type |
|---|
|
SCAN
|
22 |
128 |
7.37e-49 |
SMART |
|
KRAB
|
124 |
185 |
3.96e-2 |
SMART |
|
ZnF_C2H2
|
244 |
266 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
293 |
315 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
321 |
343 |
5.34e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000124387
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000128293
|
| SMART Domains |
Protein: ENSMUSP00000124620
Gene: ENSMUSG00000004500
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
31 |
75 |
3.83e-11 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000147996
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000128427
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.4%
|
| Validation Efficiency |
97% (37/38) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adgrb1 |
A |
C |
15: 74,416,864 (GRCm39) |
K757T |
probably damaging |
Het |
| Akr1c19 |
C |
T |
13: 4,293,032 (GRCm39) |
R263C |
probably damaging |
Het |
| Asxl1 |
A |
G |
2: 153,241,264 (GRCm39) |
T605A |
probably benign |
Het |
| Cacna1i |
A |
T |
15: 80,265,272 (GRCm39) |
|
probably benign |
Het |
| Clip4 |
A |
G |
17: 72,106,003 (GRCm39) |
D62G |
probably damaging |
Het |
| Cstf1 |
A |
G |
2: 172,222,312 (GRCm39) |
T357A |
probably benign |
Het |
| Cul5 |
T |
C |
9: 53,540,516 (GRCm39) |
K499E |
probably damaging |
Het |
| Cyld |
T |
A |
8: 89,456,179 (GRCm39) |
S407T |
probably benign |
Het |
| Dnph1 |
G |
A |
17: 46,809,637 (GRCm39) |
G101S |
possibly damaging |
Het |
| Entrep1 |
T |
C |
19: 23,956,831 (GRCm39) |
E354G |
probably benign |
Het |
| Esp38 |
A |
T |
17: 40,266,112 (GRCm39) |
R74* |
probably null |
Het |
| Fdxr |
A |
T |
11: 115,160,527 (GRCm39) |
L336Q |
probably damaging |
Het |
| Hivep1 |
T |
C |
13: 42,311,203 (GRCm39) |
S1148P |
probably benign |
Het |
| Hivep2 |
C |
A |
10: 14,004,713 (GRCm39) |
T437K |
probably benign |
Het |
| Itgb1bp2 |
T |
C |
X: 100,495,293 (GRCm39) |
|
probably benign |
Het |
| Kcnj5 |
T |
C |
9: 32,229,124 (GRCm39) |
T25A |
possibly damaging |
Het |
| Lyn |
A |
G |
4: 3,742,455 (GRCm39) |
H28R |
probably benign |
Het |
| Nbeal1 |
C |
T |
1: 60,290,572 (GRCm39) |
|
probably benign |
Het |
| Nmur2 |
A |
G |
11: 55,931,603 (GRCm39) |
L36P |
probably damaging |
Het |
| Or14j3 |
G |
A |
17: 37,900,717 (GRCm39) |
Q176* |
probably null |
Het |
| Or6c5c |
A |
T |
10: 129,298,821 (GRCm39) |
Y92F |
probably damaging |
Het |
| Or9i14 |
T |
G |
19: 13,792,712 (GRCm39) |
I81L |
probably benign |
Het |
| Pdgfra |
T |
A |
5: 75,340,881 (GRCm39) |
Y613* |
probably null |
Het |
| Phka2 |
T |
C |
X: 159,316,045 (GRCm39) |
V230A |
possibly damaging |
Het |
| Pkd2l1 |
A |
G |
19: 44,145,666 (GRCm39) |
S186P |
probably damaging |
Het |
| Skic3 |
T |
C |
13: 76,261,798 (GRCm39) |
I171T |
probably benign |
Het |
| Snx14 |
T |
C |
9: 88,302,296 (GRCm39) |
|
probably benign |
Het |
| Tex15 |
T |
C |
8: 34,064,194 (GRCm39) |
V1208A |
probably benign |
Het |
| Tmf1 |
A |
G |
6: 97,149,292 (GRCm39) |
F485S |
possibly damaging |
Het |
| Tnfsf4 |
G |
A |
1: 161,244,778 (GRCm39) |
V156I |
possibly damaging |
Het |
| Trdn |
C |
A |
10: 33,210,980 (GRCm39) |
Q391K |
probably damaging |
Het |
| Ttc34 |
T |
C |
4: 154,949,939 (GRCm39) |
F964S |
probably damaging |
Het |
| Vmn2r104 |
A |
G |
17: 20,249,818 (GRCm39) |
S818P |
probably damaging |
Het |
| Zbed5 |
G |
A |
5: 129,932,000 (GRCm39) |
D650N |
possibly damaging |
Het |
| Zfp592 |
C |
T |
7: 80,687,159 (GRCm39) |
R821* |
probably null |
Het |
| Zfp647 |
G |
A |
15: 76,795,110 (GRCm39) |
R517W |
probably damaging |
Het |
| Zfp983 |
G |
C |
17: 21,880,455 (GRCm39) |
E128Q |
probably damaging |
Het |
|
| Other mutations in Zfp446 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01486:Zfp446
|
APN |
7 |
12,713,307 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01534:Zfp446
|
APN |
7 |
12,713,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02471:Zfp446
|
APN |
7 |
12,716,181 (GRCm39) |
missense |
probably benign |
0.41 |
|
IGL02888:Zfp446
|
APN |
7 |
12,713,255 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03153:Zfp446
|
APN |
7 |
12,711,834 (GRCm39) |
missense |
probably benign |
0.01 |
|
IGL03185:Zfp446
|
APN |
7 |
12,712,925 (GRCm39) |
missense |
probably null |
0.43 |
|
IGL03242:Zfp446
|
APN |
7 |
12,713,252 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0538:Zfp446
|
UTSW |
7 |
12,713,516 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R1809:Zfp446
|
UTSW |
7 |
12,713,048 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3686:Zfp446
|
UTSW |
7 |
12,716,580 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5256:Zfp446
|
UTSW |
7 |
12,713,231 (GRCm39) |
nonsense |
probably null |
|
|
R5363:Zfp446
|
UTSW |
7 |
12,711,984 (GRCm39) |
missense |
probably benign |
0.20 |
|
R5377:Zfp446
|
UTSW |
7 |
12,716,178 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R6806:Zfp446
|
UTSW |
7 |
12,713,043 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7167:Zfp446
|
UTSW |
7 |
12,712,049 (GRCm39) |
unclassified |
probably benign |
|
|
R7988:Zfp446
|
UTSW |
7 |
12,712,970 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R8288:Zfp446
|
UTSW |
7 |
12,711,885 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8487:Zfp446
|
UTSW |
7 |
12,716,555 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R8943:Zfp446
|
UTSW |
7 |
12,713,564 (GRCm39) |
nonsense |
probably null |
|
|
R9315:Zfp446
|
UTSW |
7 |
12,713,397 (GRCm39) |
missense |
probably benign |
0.28 |
|
Z1177:Zfp446
|
UTSW |
7 |
12,712,093 (GRCm39) |
unclassified |
probably benign |
|
|
| Predicted Primers |
|
| Posted On |
2017-05-11 |
Incidental Mutation