Mutagenetix > Incidental Mutations

Incidental Mutation 'R3103:Zfp616'

477725

Institutional Source

Beutler Lab

Gene Symbol

Zfp616

Ensembl Gene

ENSMUSG00000069476

Gene Name

zinc finger protein 616

Synonyms

Gm12330

MMRRC Submission

040577-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3103 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

74069955-74087292 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 74071735 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 74 (T74A)

Ref Sequence

ENSEMBL: ENSMUSP00000112245 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074813] [ENSMUST00000108463] [ENSMUST00000116546]

Predicted Effect

probably benign
Transcript: ENSMUST00000074813
AA Change: T74A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000074365
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108463
AA Change: T74A

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000104103
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART
low complexity region	249	258	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000116546
AA Change: T74A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000112245
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137407

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acp7	A	G	7: 28,610,984		probably null	Het
Adap2	G	T	11: 80,157,033	C105F	probably damaging	Het
Bace2	T	C	16: 97,422,001		probably null	Het
Bpifc	A	G	10: 85,993,422	S94P	probably damaging	Het
C2cd3	A	G	7: 100,395,252	D347G	possibly damaging	Het
Cad	T	C	5: 31,061,674	V613A	possibly damaging	Het
Ccdc47	T	C	11: 106,202,841	H6R	probably benign	Het
Celsr3	A	G	9: 108,837,139	T1956A	probably benign	Het
Cep128	T	A	12: 91,019,344	D1006V	probably damaging	Het
Cog3	T	C	14: 75,747,201		probably null	Het
Csmd1	C	T	8: 15,917,405	V3153M	probably damaging	Het
Ctnna2	T	C	6: 77,653,144	E122G	possibly damaging	Het
Cts8	T	A	13: 61,250,958	I245F	probably damaging	Het
Ddx27	T	A	2: 167,026,246	V333E	probably damaging	Het
Dmpk	A	G	7: 19,087,654	Y279C	probably damaging	Het
Dpagt1	A	G	9: 44,327,995	I111V	probably benign	Het
Dvl2	C	A	11: 70,008,869	P546T	possibly damaging	Het
Fat4	G	T	3: 38,891,940	A1661S	probably benign	Het
Gcm1	A	G	9: 78,064,452	N225S	probably damaging	Het
Gcnt2	A	T	13: 40,918,606	M242L	probably benign	Het
Golgb1	A	G	16: 36,894,849	R226G	probably damaging	Het
Gpr63	G	A	4: 25,007,353	V26I	probably benign	Het
Grik2	A	G	10: 49,240,772	L631P	probably damaging	Het
Hapln3	T	C	7: 79,121,736	D135G	probably benign	Het
Il31ra	C	A	13: 112,530,351	V398F	probably damaging	Het
Ipp	G	T	4: 116,524,249	R315L	possibly damaging	Het
Kcmf1	A	G	6: 72,861,847	L32P	probably damaging	Het
Klf17	T	A	4: 117,760,608	Q184L	possibly damaging	Het
Lrp2	C	T	2: 69,431,984	V4442I	probably benign	Het
Lrrfip2	A	T	9: 111,222,210	E293D	probably damaging	Het
Oit3	A	G	10: 59,438,891	I29T	probably damaging	Het
Olfr403	G	A	11: 74,196,075	D191N	probably benign	Het
Olfr50	T	C	2: 36,793,562	C109R	possibly damaging	Het
Olfr555	T	C	7: 102,659,481	V220A	probably benign	Het
Plb1	T	A	5: 32,328,029	M842K	possibly damaging	Het
Ppt1	T	C	4: 122,836,307	C18R	probably benign	Het
Pstpip2	T	C	18: 77,871,777	Y191H	probably damaging	Het
Ryr1	C	T	7: 29,074,948	V2361I	probably damaging	Het
Serpinb11	A	G	1: 107,377,608	N238S	probably benign	Het
Skor2	A	T	18: 76,859,278	K232*	probably null	Het
Slc13a5	A	T	11: 72,257,388	W231R	probably damaging	Het
Svs5	A	T	2: 164,333,393	E55V	probably benign	Het
Tfcp2	A	T	15: 100,525,600	W142R	probably damaging	Het
Trpc2	A	T	7: 102,095,234	I738F	possibly damaging	Het
Vmn2r61	T	A	7: 42,266,643	S227T	possibly damaging	Het
Zfhx4	A	G	3: 5,399,326	T1540A	probably damaging	Het

Other mutations in Zfp616

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00530:Zfp616	APN	11	74083613	missense	probably damaging	0.98
IGL00570:Zfp616	APN	11	74085805	missense	probably benign	0.03
IGL00594:Zfp616	APN	11	74082963	missense	possibly damaging	0.72
IGL01861:Zfp616	APN	11	74082916	missense	possibly damaging	0.53
IGL03022:Zfp616	APN	11	74082974	missense	possibly damaging	0.85
R0197:Zfp616	UTSW	11	74085674	missense	probably damaging	1.00
R0442:Zfp616	UTSW	11	74084495	missense	possibly damaging	0.92
R0497:Zfp616	UTSW	11	74083480	missense	probably benign	0.00
R0651:Zfp616	UTSW	11	74083729	nonsense	probably null
R0730:Zfp616	UTSW	11	74084822	missense	probably damaging	1.00
R0883:Zfp616	UTSW	11	74085674	missense	probably damaging	1.00
R0926:Zfp616	UTSW	11	74085818	missense	probably benign	0.04
R0940:Zfp616	UTSW	11	74085024	missense	probably damaging	1.00
R1068:Zfp616	UTSW	11	74082941	makesense	probably null
R1272:Zfp616	UTSW	11	74085236	missense	probably benign	0.08
R1446:Zfp616	UTSW	11	74083238	unclassified	probably null
R1482:Zfp616	UTSW	11	74083977	missense	possibly damaging	0.72
R1553:Zfp616	UTSW	11	74083918	missense	possibly damaging	0.53
R1564:Zfp616	UTSW	11	74084722	missense	probably damaging	1.00
R1728:Zfp616	UTSW	11	74085771	missense	probably damaging	0.99
R1796:Zfp616	UTSW	11	74085845	missense	probably damaging	0.98
R1797:Zfp616	UTSW	11	74085279	nonsense	probably null
R1993:Zfp616	UTSW	11	74084969	missense	probably benign	0.08
R2026:Zfp616	UTSW	11	74083587	missense	possibly damaging	0.86
R2124:Zfp616	UTSW	11	74083043	unclassified	probably null
R2126:Zfp616	UTSW	11	74085403	missense	probably benign	0.08
R2199:Zfp616	UTSW	11	74084630	missense	possibly damaging	0.58
R2265:Zfp616	UTSW	11	74085463	missense	possibly damaging	0.89
R2404:Zfp616	UTSW	11	74084856	missense	probably damaging	1.00
R2508:Zfp616	UTSW	11	74083295	missense	probably benign	0.01
R2519:Zfp616	UTSW	11	74084268	nonsense	probably null
R3611:Zfp616	UTSW	11	74083442	missense	possibly damaging	0.53
R3703:Zfp616	UTSW	11	74083319	nonsense	probably null
R3744:Zfp616	UTSW	11	74083987	missense	probably benign	0.01
R4043:Zfp616	UTSW	11	74085282	missense	possibly damaging	0.50
R4273:Zfp616	UTSW	11	74083700	missense	probably benign	0.00
R4384:Zfp616	UTSW	11	74083179	missense	possibly damaging	0.94
R4469:Zfp616	UTSW	11	74071124	missense	probably damaging	0.98
R4560:Zfp616	UTSW	11	74083034	missense	probably benign	0.00
R4821:Zfp616	UTSW	11	74084207	missense	probably benign	0.41
R4844:Zfp616	UTSW	11	74084399	missense	probably benign	0.10
R4948:Zfp616	UTSW	11	74084004	missense	possibly damaging	0.72
R5007:Zfp616	UTSW	11	74083817	missense	possibly damaging	0.96
R5198:Zfp616	UTSW	11	74083510	missense	probably benign	0.33
R5344:Zfp616	UTSW	11	74084495	missense	possibly damaging	0.92
R5918:Zfp616	UTSW	11	74083260	missense	possibly damaging	0.70
R5933:Zfp616	UTSW	11	74083126	missense	probably damaging	0.96
R6084:Zfp616	UTSW	11	74083846	nonsense	probably null
R6421:Zfp616	UTSW	11	74083870	missense	possibly damaging	0.53
R6494:Zfp616	UTSW	11	74085192	missense	probably damaging	1.00
R6523:Zfp616	UTSW	11	74083142	missense	possibly damaging	0.79
R6849:Zfp616	UTSW	11	74085450	missense	possibly damaging	0.70
R6910:Zfp616	UTSW	11	74085002	missense	probably damaging	1.00
R7146:Zfp616	UTSW	11	74085261	missense	possibly damaging	0.61
R7213:Zfp616	UTSW	11	74085863	missense	probably benign	0.05
R7302:Zfp616	UTSW	11	74085379	missense	probably benign	0.08
R7391:Zfp616	UTSW	11	74085329	missense	probably benign	0.08
R7654:Zfp616	UTSW	11	74083187	missense	possibly damaging	0.53
R7877:Zfp616	UTSW	11	74084362	missense	probably damaging	1.00
R7889:Zfp616	UTSW	11	74085445	missense	probably damaging	1.00
R7960:Zfp616	UTSW	11	74084362	missense	probably damaging	1.00
R7972:Zfp616	UTSW	11	74085445	missense	probably damaging	1.00
R8022:Zfp616	UTSW	11	74084068	missense	not run
R8061:Zfp616	UTSW	11	74083514	missense	not run
Z1176:Zfp616	UTSW	11	74083033	missense	not run
Z1176:Zfp616	UTSW	11	74083219	missense	not run
Z1176:Zfp616	UTSW	11	74085641	missense	not run
Z1177:Zfp616	UTSW	11	74085052	missense	not run

Predicted Primers

Posted On

2017-05-15