Mutagenetix > Incidental Mutation

Incidental Mutation 'R3103:Zfp616'

477725

Institutional Source

Beutler Lab

Gene Symbol

Zfp616

Ensembl Gene

ENSMUSG00000069476

Gene Name

zinc finger protein 616

Synonyms

Gm12330

MMRRC Submission

040577-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3103 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73960781-73978118 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 73962561 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 74 (T74A)

Ref Sequence

ENSEMBL: ENSMUSP00000112245 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074813] [ENSMUST00000108463] [ENSMUST00000116546]

AlphaFold

J3QN14

Predicted Effect

probably benign
Transcript: ENSMUST00000074813
AA Change: T74A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000074365
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108463
AA Change: T74A

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000104103
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART
low complexity region	249	258	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000116546
AA Change: T74A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000112245
Gene: ENSMUSG00000069476
AA Change: T74A

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137407

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acp7	A	G	7: 28,310,409 (GRCm39)		probably null	Het
Adap2	G	T	11: 80,047,859 (GRCm39)	C105F	probably damaging	Het
Bace2	T	C	16: 97,223,201 (GRCm39)		probably null	Het
Bpifc	A	G	10: 85,829,286 (GRCm39)	S94P	probably damaging	Het
C2cd3	A	G	7: 100,044,459 (GRCm39)	D347G	possibly damaging	Het
Cad	T	C	5: 31,219,018 (GRCm39)	V613A	possibly damaging	Het
Ccdc47	T	C	11: 106,093,667 (GRCm39)	H6R	probably benign	Het
Celsr3	A	G	9: 108,714,338 (GRCm39)	T1956A	probably benign	Het
Cep128	T	A	12: 90,986,118 (GRCm39)	D1006V	probably damaging	Het
Cog3	T	C	14: 75,984,641 (GRCm39)		probably null	Het
Csmd1	C	T	8: 15,967,405 (GRCm39)	V3153M	probably damaging	Het
Ctnna2	T	C	6: 77,630,127 (GRCm39)	E122G	possibly damaging	Het
Cts8	T	A	13: 61,398,772 (GRCm39)	I245F	probably damaging	Het
Ddx27	T	A	2: 166,868,166 (GRCm39)	V333E	probably damaging	Het
Dmpk	A	G	7: 18,821,579 (GRCm39)	Y279C	probably damaging	Het
Dpagt1	A	G	9: 44,239,292 (GRCm39)	I111V	probably benign	Het
Dvl2	C	A	11: 69,899,695 (GRCm39)	P546T	possibly damaging	Het
Fat4	G	T	3: 38,946,089 (GRCm39)	A1661S	probably benign	Het
Gcm1	A	G	9: 77,971,734 (GRCm39)	N225S	probably damaging	Het
Gcnt2	A	T	13: 41,072,082 (GRCm39)	M242L	probably benign	Het
Golgb1	A	G	16: 36,715,211 (GRCm39)	R226G	probably damaging	Het
Gpr63	G	A	4: 25,007,353 (GRCm39)	V26I	probably benign	Het
Grik2	A	G	10: 49,116,868 (GRCm39)	L631P	probably damaging	Het
Hapln3	T	C	7: 78,771,484 (GRCm39)	D135G	probably benign	Het
Il31ra	C	A	13: 112,666,885 (GRCm39)	V398F	probably damaging	Het
Ipp	G	T	4: 116,381,446 (GRCm39)	R315L	possibly damaging	Het
Kcmf1	A	G	6: 72,838,830 (GRCm39)	L32P	probably damaging	Het
Klf17	T	A	4: 117,617,805 (GRCm39)	Q184L	possibly damaging	Het
Lrp2	C	T	2: 69,262,328 (GRCm39)	V4442I	probably benign	Het
Lrrfip2	A	T	9: 111,051,278 (GRCm39)	E293D	probably damaging	Het
Oit3	A	G	10: 59,274,713 (GRCm39)	I29T	probably damaging	Het
Or1a1	G	A	11: 74,086,901 (GRCm39)	D191N	probably benign	Het
Or1j21	T	C	2: 36,683,574 (GRCm39)	C109R	possibly damaging	Het
Or51h1	T	C	7: 102,308,688 (GRCm39)	V220A	probably benign	Het
Plb1	T	A	5: 32,485,373 (GRCm39)	M842K	possibly damaging	Het
Ppt1	T	C	4: 122,730,100 (GRCm39)	C18R	probably benign	Het
Pstpip2	T	C	18: 77,959,477 (GRCm39)	Y191H	probably damaging	Het
Ryr1	C	T	7: 28,774,373 (GRCm39)	V2361I	probably damaging	Het
Serpinb11	A	G	1: 107,305,338 (GRCm39)	N238S	probably benign	Het
Skor2	A	T	18: 76,946,973 (GRCm39)	K232*	probably null	Het
Slc13a5	A	T	11: 72,148,214 (GRCm39)	W231R	probably damaging	Het
Svs5	A	T	2: 164,175,313 (GRCm39)	E55V	probably benign	Het
Tfcp2	A	T	15: 100,423,481 (GRCm39)	W142R	probably damaging	Het
Trpc2	A	T	7: 101,744,441 (GRCm39)	I738F	possibly damaging	Het
Vmn2r61	T	A	7: 41,916,067 (GRCm39)	S227T	possibly damaging	Het
Zfhx4	A	G	3: 5,464,386 (GRCm39)	T1540A	probably damaging	Het

Other mutations in Zfp616

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00530:Zfp616	APN	11	73,974,439 (GRCm39)	missense	probably damaging	0.98
IGL00570:Zfp616	APN	11	73,976,631 (GRCm39)	missense	probably benign	0.03
IGL00594:Zfp616	APN	11	73,973,789 (GRCm39)	missense	possibly damaging	0.72
IGL01861:Zfp616	APN	11	73,973,742 (GRCm39)	missense	possibly damaging	0.53
IGL03022:Zfp616	APN	11	73,973,800 (GRCm39)	missense	possibly damaging	0.85
R0197:Zfp616	UTSW	11	73,976,500 (GRCm39)	missense	probably damaging	1.00
R0442:Zfp616	UTSW	11	73,975,321 (GRCm39)	missense	possibly damaging	0.92
R0497:Zfp616	UTSW	11	73,974,306 (GRCm39)	missense	probably benign	0.00
R0651:Zfp616	UTSW	11	73,974,555 (GRCm39)	nonsense	probably null
R0730:Zfp616	UTSW	11	73,975,648 (GRCm39)	missense	probably damaging	1.00
R0883:Zfp616	UTSW	11	73,976,500 (GRCm39)	missense	probably damaging	1.00
R0926:Zfp616	UTSW	11	73,976,644 (GRCm39)	missense	probably benign	0.04
R0940:Zfp616	UTSW	11	73,975,850 (GRCm39)	missense	probably damaging	1.00
R1068:Zfp616	UTSW	11	73,973,767 (GRCm39)	makesense	probably null
R1272:Zfp616	UTSW	11	73,976,062 (GRCm39)	missense	probably benign	0.08
R1446:Zfp616	UTSW	11	73,974,064 (GRCm39)	splice site	probably null
R1482:Zfp616	UTSW	11	73,974,803 (GRCm39)	missense	possibly damaging	0.72
R1553:Zfp616	UTSW	11	73,974,744 (GRCm39)	missense	possibly damaging	0.53
R1564:Zfp616	UTSW	11	73,975,548 (GRCm39)	missense	probably damaging	1.00
R1728:Zfp616	UTSW	11	73,976,597 (GRCm39)	missense	probably damaging	0.99
R1796:Zfp616	UTSW	11	73,976,671 (GRCm39)	missense	probably damaging	0.98
R1797:Zfp616	UTSW	11	73,976,105 (GRCm39)	nonsense	probably null
R1993:Zfp616	UTSW	11	73,975,795 (GRCm39)	missense	probably benign	0.08
R2026:Zfp616	UTSW	11	73,974,413 (GRCm39)	missense	possibly damaging	0.86
R2124:Zfp616	UTSW	11	73,973,869 (GRCm39)	splice site	probably null
R2126:Zfp616	UTSW	11	73,976,229 (GRCm39)	missense	probably benign	0.08
R2199:Zfp616	UTSW	11	73,975,456 (GRCm39)	missense	possibly damaging	0.58
R2265:Zfp616	UTSW	11	73,976,289 (GRCm39)	missense	possibly damaging	0.89
R2404:Zfp616	UTSW	11	73,975,682 (GRCm39)	missense	probably damaging	1.00
R2508:Zfp616	UTSW	11	73,974,121 (GRCm39)	missense	probably benign	0.01
R2519:Zfp616	UTSW	11	73,975,094 (GRCm39)	nonsense	probably null
R3611:Zfp616	UTSW	11	73,974,268 (GRCm39)	missense	possibly damaging	0.53
R3703:Zfp616	UTSW	11	73,974,145 (GRCm39)	nonsense	probably null
R3744:Zfp616	UTSW	11	73,974,813 (GRCm39)	missense	probably benign	0.01
R4043:Zfp616	UTSW	11	73,976,108 (GRCm39)	missense	possibly damaging	0.50
R4273:Zfp616	UTSW	11	73,974,526 (GRCm39)	missense	probably benign	0.00
R4384:Zfp616	UTSW	11	73,974,005 (GRCm39)	missense	possibly damaging	0.94
R4469:Zfp616	UTSW	11	73,961,950 (GRCm39)	missense	probably damaging	0.98
R4560:Zfp616	UTSW	11	73,973,860 (GRCm39)	missense	probably benign	0.00
R4821:Zfp616	UTSW	11	73,975,033 (GRCm39)	missense	probably benign	0.41
R4844:Zfp616	UTSW	11	73,975,225 (GRCm39)	missense	probably benign	0.10
R4948:Zfp616	UTSW	11	73,974,830 (GRCm39)	missense	possibly damaging	0.72
R5007:Zfp616	UTSW	11	73,974,643 (GRCm39)	missense	possibly damaging	0.96
R5198:Zfp616	UTSW	11	73,974,336 (GRCm39)	missense	probably benign	0.33
R5344:Zfp616	UTSW	11	73,975,321 (GRCm39)	missense	possibly damaging	0.92
R5918:Zfp616	UTSW	11	73,974,086 (GRCm39)	missense	possibly damaging	0.70
R5933:Zfp616	UTSW	11	73,973,952 (GRCm39)	missense	probably damaging	0.96
R6084:Zfp616	UTSW	11	73,974,672 (GRCm39)	nonsense	probably null
R6421:Zfp616	UTSW	11	73,974,696 (GRCm39)	missense	possibly damaging	0.53
R6494:Zfp616	UTSW	11	73,976,018 (GRCm39)	missense	probably damaging	1.00
R6523:Zfp616	UTSW	11	73,973,968 (GRCm39)	missense	possibly damaging	0.79
R6849:Zfp616	UTSW	11	73,976,276 (GRCm39)	missense	possibly damaging	0.70
R6910:Zfp616	UTSW	11	73,975,828 (GRCm39)	missense	probably damaging	1.00
R7146:Zfp616	UTSW	11	73,976,087 (GRCm39)	missense	possibly damaging	0.61
R7213:Zfp616	UTSW	11	73,976,689 (GRCm39)	missense	probably benign	0.05
R7302:Zfp616	UTSW	11	73,976,205 (GRCm39)	missense	probably benign	0.08
R7391:Zfp616	UTSW	11	73,976,155 (GRCm39)	missense	probably benign	0.08
R7654:Zfp616	UTSW	11	73,974,013 (GRCm39)	missense	possibly damaging	0.53
R7877:Zfp616	UTSW	11	73,975,188 (GRCm39)	missense	probably damaging	1.00
R7889:Zfp616	UTSW	11	73,976,271 (GRCm39)	missense	probably damaging	1.00
R8022:Zfp616	UTSW	11	73,974,894 (GRCm39)	missense	probably benign
R8061:Zfp616	UTSW	11	73,974,340 (GRCm39)	missense	possibly damaging	0.96
R8212:Zfp616	UTSW	11	73,976,569 (GRCm39)	missense	probably damaging	0.96
R8335:Zfp616	UTSW	11	73,974,726 (GRCm39)	nonsense	probably null
R8361:Zfp616	UTSW	11	73,975,476 (GRCm39)	missense	probably damaging	0.98
R8486:Zfp616	UTSW	11	73,974,909 (GRCm39)	missense	probably benign	0.18
R8695:Zfp616	UTSW	11	73,975,710 (GRCm39)	missense	probably benign	0.45
R8808:Zfp616	UTSW	11	73,976,523 (GRCm39)	missense	probably damaging	1.00
R9022:Zfp616	UTSW	11	73,976,539 (GRCm39)	missense	probably damaging	1.00
R9126:Zfp616	UTSW	11	73,976,280 (GRCm39)	missense	probably damaging	1.00
R9164:Zfp616	UTSW	11	73,975,825 (GRCm39)	missense	probably damaging	1.00
R9293:Zfp616	UTSW	11	73,974,744 (GRCm39)	missense	possibly damaging	0.53
R9421:Zfp616	UTSW	11	73,974,331 (GRCm39)	missense	possibly damaging	0.72
R9512:Zfp616	UTSW	11	73,975,936 (GRCm39)	missense	probably damaging	1.00
R9529:Zfp616	UTSW	11	73,976,596 (GRCm39)	missense	possibly damaging	0.90
R9529:Zfp616	UTSW	11	73,975,660 (GRCm39)	missense	probably damaging	1.00
R9606:Zfp616	UTSW	11	73,976,220 (GRCm39)	missense	probably damaging	1.00
R9708:Zfp616	UTSW	11	73,976,283 (GRCm39)	missense	probably damaging	1.00
R9788:Zfp616	UTSW	11	73,975,276 (GRCm39)	missense	probably damaging	0.99
Z1176:Zfp616	UTSW	11	73,976,467 (GRCm39)	missense	possibly damaging	0.90
Z1176:Zfp616	UTSW	11	73,974,045 (GRCm39)	missense	possibly damaging	0.72
Z1176:Zfp616	UTSW	11	73,973,859 (GRCm39)	missense	probably benign
Z1177:Zfp616	UTSW	11	73,975,878 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

Posted On

2017-05-15