Mutagenetix > Incidental Mutation

Incidental Mutation 'R5306:Ifit3'

478097

Institutional Source

Beutler Lab

Gene Symbol

Ifit3

Ensembl Gene

ENSMUSG00000074896

Gene Name

interferon-induced protein with tetratricopeptide repeats 3

Synonyms

Ifi49

MMRRC Submission

042889-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5306 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

34583531-34588731 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 34587807 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 251 (Y251C)

Ref Sequence

ENSEMBL: ENSMUSP00000099889 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102825]

AlphaFold

Q64345

Predicted Effect

probably damaging
Transcript: ENSMUST00000102825
AA Change: Y251C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000099889
Gene: ENSMUSG00000074896
AA Change: Y251C

Domain	Start	End	E-Value	Type
TPR	51	84	7.69e1	SMART
TPR	94	127	2.84e1	SMART
TPR	136	169	5.69e0	SMART
Blast:TPR	170	206	6e-6	BLAST
low complexity region	209	218	N/A	INTRINSIC
TPR	241	274	1.02e1	SMART

Meta Mutation Damage Score

0.9408

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.2%

Validation Efficiency

97% (64/66)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930021J03Rik	A	G	19: 29,729,830		probably benign	Het
Adam29	T	A	8: 55,871,757	D554V	probably damaging	Het
Ankrd44	G	A	1: 54,926,203		probably benign	Het
Api5	A	T	2: 94,423,466	C297*	probably null	Het
Asb14	G	A	14: 26,911,909	C357Y	probably damaging	Het
Brdt	T	A	5: 107,345,144	D112E	probably damaging	Het
Capsl	C	A	15: 9,457,790	Q32K	probably benign	Het
Ccdc106	A	G	7: 5,058,097	D81G	probably damaging	Het
Cep104	C	A	4: 154,006,242	T884K	probably benign	Het
Cmbl	T	C	15: 31,582,069	Y71H	probably damaging	Het
Crybg3	A	T	16: 59,559,993		probably benign	Het
Dynlt1c	T	C	17: 6,601,811	M1T	probably null	Het
Erbb2	T	C	11: 98,428,206	S574P	probably benign	Het
Exosc10	T	C	4: 148,562,392	V153A	probably benign	Het
Faxc	G	T	4: 21,931,557		probably benign	Het
Fcgbp	A	G	7: 28,091,818	T835A	probably damaging	Het
Fmo5	G	T	3: 97,641,760	M241I	probably benign	Het
Gabra1	A	C	11: 42,133,552	I432S	probably benign	Het
Gfap	A	G	11: 102,895,748		probably null	Het
Gm11595	G	A	11: 99,772,555	R100C	unknown	Het
Gm12185	T	A	11: 48,915,555	M270L	probably benign	Het
Gm14443	T	C	2: 175,169,579	N358S	possibly damaging	Het
Gm6583	T	C	5: 112,355,044	R265G	probably benign	Het
Gpr3	C	T	4: 133,211,179	V61M	probably damaging	Het
Herc2	C	T	7: 56,184,961	T3229M	probably damaging	Het
Inf2	G	A	12: 112,601,553	V180I	probably benign	Het
Ints11	T	C	4: 155,875,208	Y91H	probably damaging	Het
Ints4	A	C	7: 97,509,678	D419A	probably damaging	Het
Kmt2e	T	C	5: 23,499,333	S1175P	probably damaging	Het
Mki67	A	T	7: 135,714,001	V44E	probably damaging	Het
Mrgprb13	A	T	7: 48,312,192		noncoding transcript	Het
Myh2	T	C	11: 67,186,556	L839P	probably damaging	Het
Nos1ap	T	A	1: 170,349,399	K145M	probably damaging	Het
Olfr723	A	G	14: 49,929,550		probably benign	Het
Olfr814	A	C	10: 129,873,941	I272R	probably damaging	Het
Pced1a	T	A	2: 130,419,171	H422L	probably benign	Het
Plpp1	G	T	13: 112,851,555		probably null	Het
Plxna4	T	C	6: 32,206,121	Y949C	probably damaging	Het
Polg2	G	A	11: 106,778,970	T158I	probably damaging	Het
Prss38	A	T	11: 59,372,995	I297K	probably benign	Het
Psph	A	G	5: 129,769,367	L98P	probably damaging	Het
Rab11b	G	A	17: 33,760,269		probably benign	Het
Rsf1	CGGCGGC	CGGCGGCGGGGGCGGC	7: 97,579,929		probably benign	Het
Serpine3	G	A	14: 62,670,933	A137T	probably damaging	Het
Sh3bp4	T	C	1: 89,144,275	F282L	probably damaging	Het
Sh3bp5	C	A	14: 31,377,495	R265L	probably benign	Het
Slco2b1	T	A	7: 99,688,991	Y109F	possibly damaging	Het
Slfn10-ps	T	A	11: 83,035,529		noncoding transcript	Het
Smc5	A	G	19: 23,259,645		probably null	Het
Smyd4	A	G	11: 75,402,158	N638S	probably benign	Het
Stxbp5	T	C	10: 9,799,991	E628G	probably damaging	Het
Tmem236	A	T	2: 14,219,164	K255*	probably null	Het
Ttc29	T	A	8: 78,251,910		probably null	Het
Ttc37	G	A	13: 76,147,767	E1050K	possibly damaging	Het
Tyr	A	G	7: 87,438,014	I430T	probably damaging	Het
Uckl1	A	G	2: 181,574,367		probably null	Het
Vmn2r52	A	C	7: 10,170,745	I389R	possibly damaging	Het
Wdr62	A	T	7: 30,265,263	F352Y	possibly damaging	Het
Wdr70	T	C	15: 7,924,273	D379G	probably benign	Het
Zfp408	T	A	2: 91,646,345	M155L	probably benign	Het
Zfp459	T	A	13: 67,413,130	Q66H	probably damaging	Het
Zfp870	C	A	17: 32,883,653	G234V	probably damaging	Het

Other mutations in Ifit3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1501:Ifit3	UTSW	19	34588251	missense	probably benign	0.13
R1521:Ifit3	UTSW	19	34587173	missense	probably damaging	1.00
R3084:Ifit3	UTSW	19	34587240	missense	probably damaging	0.99
R5017:Ifit3	UTSW	19	34587192	missense	possibly damaging	0.78
R6194:Ifit3	UTSW	19	34587627	missense	probably benign	0.06
R6523:Ifit3	UTSW	19	34588155	missense	probably benign	0.10
R6559:Ifit3	UTSW	19	34587114	missense	probably damaging	1.00
R7535:Ifit3	UTSW	19	34587880	missense	probably damaging	0.98
R7947:Ifit3	UTSW	19	34587959	nonsense	probably null
R8049:Ifit3	UTSW	19	34588080	missense	possibly damaging	0.88
R8142:Ifit3	UTSW	19	34587501	missense	probably damaging	1.00
R8850:Ifit3	UTSW	19	34587588	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGAAGCAGTTCTCCGTGGATG -3'
(R):5'- AGTCTTCTGCATGAACTCCATC -3'

Sequencing Primer
(F):5'- CTCCGTGGATGCTCTGAAG -3'
(R):5'- GCATGAACTCCATCGTTAATCG -3'

Posted On

2017-05-23