Mutagenetix > Incidental Mutation

Incidental Mutation 'LCD18:Zfp442'

478157

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

LCD18 (G1)

Quality Score

999

Status

Validated

Chromosome

Chromosomal Location

150407141-150451486 bp(-) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

C to T at 150419848 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916]

AlphaFold

A2AQA0

Predicted Effect

probably benign
Transcript: ENSMUST00000109916

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

88% (169/191)

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700007G11Rik	G	A	5: 98,707,508		probably benign	Het
4930548H24Rik	GAGAAG	GAG	5: 31,487,373		probably benign	Het
9530077C05Rik	N		9: 22,442,083		probably benign	Het
A330008L17Rik	A	G	8: 99,723,425		noncoding transcript	Het
Aaed1	G	A	13: 64,287,285		probably benign	Het
Aff2	C	A	X: 69,747,535		probably benign	Het
Aldh1a1	C	A	19: 20,626,646		probably benign	Het
Anxa7	C	T	14: 20,469,411	G113E	probably damaging	Het
Apba2	A	T	7: 64,622,160		probably benign	Het
Apc2	G	C	10: 80,299,974		probably benign	Het
App	C	G	16: 85,025,412		probably benign	Het
Asic2	C	A	11: 80,985,744		probably benign	Het
Btk	T	C	X: 134,578,825		probably benign	Het
C530008M17Rik	GGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	GGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	5: 76,658,742		probably benign	Het
Car12	C	A	9: 66,761,676		probably benign	Het
Ccdc191	G	A	16: 43,921,801		probably benign	Het
Ccdc34	N		2: 110,016,318		probably benign	Het
Cd164	G	T	10: 41,521,926	A59S	probably benign	Het
Cd22	C	T	7: 30,878,082	R2H	possibly damaging	Het
Cdv3	A	T	9: 103,365,343		probably benign	Het
Cdv3	C	A	9: 103,365,354		probably benign	Het
Celf2	N		2: 6,779,076		probably benign	Het
Clec18a	C	A	8: 111,076,136		probably benign	Het
Cnpy3	GGATGGAT	GGATAGATAGATAGATAGATGGAT	17: 46,737,536		probably benign	Het
Cntn4	A	G	6: 106,553,940		probably benign	Het
Cntnap5c	G	C	17: 58,162,160		probably benign	Het
Col2a1	C	A	15: 97,988,981		probably null	Het
Cpne3	G	T	4: 19,563,382		probably benign	Het
Dab1	T	G	4: 104,046,572		probably benign	Het
Dapp1	G	A	3: 137,939,400		probably benign	Het
Dcc	G	A	18: 72,297,447		probably benign	Het
Dcun1d1	GAAAAAAAAA	GAAAAAAAAAA	3: 35,938,005		probably benign	Het
Dennd1b	G	A	1: 139,114,764		probably benign	Het
Dhdds	TAA	TA	4: 133,970,363		probably benign	Het
Dnah12	G	T	14: 26,849,385	G2817V	probably damaging	Het
Dnm3	CATATATATATATATATATATATA	CATATATATATATATATATATA	1: 162,406,561		probably benign	Het
Dock10	N		1: 80,716,623		probably benign	Het
Dusp10	G	T	1: 184,037,056	C73F	probably damaging	Het
Fgf20	A	C	8: 40,292,318		probably benign	Het
Ftsj3	G	T	11: 106,250,059		probably benign	Het
Gls	T	G	1: 52,183,367		probably benign	Het
Gm12130	T	C	11: 38,506,923		noncoding transcript	Het
Gm12394	T	C	4: 42,792,885	T416A	probably benign	Het
Gm14936	G	A	X: 112,998,750		noncoding transcript	Het
Gm16630	C	T	6: 48,141,269		noncoding transcript	Het
Gm22194	AGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	AGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	10: 11,940,963		noncoding transcript	Het
Gm26917	C	G	17: 39,843,971		noncoding transcript	Het
Gm35048	GACACACACACACACACACACACACACACACACACACAC	GACACACACACACACACACACACACACACACACACAC	1: 90,521,526		noncoding transcript	Het
Gm37311	G	A	16: 77,618,281		noncoding transcript	Het
Gm37928	AACACACACACACACACACACACACACACACACA	AACACACACACACACACACACACACACACACACACA	3: 118,534,557		noncoding transcript	Het
Gm42418	T	C	17: 39,848,555		noncoding transcript	Het
Gm4302	T	C	10: 100,341,444	W197R	probably benign	Het
Gm5615	T	C	9: 36,533,553		probably benign	Het
H2-Q4	G	A	17: 35,380,405	D155N	probably damaging	Het
H2-T23	T	C	17: 36,031,216		probably benign	Het
Hgs	CTTTTTTT	CTTTTTT	11: 120,469,578		probably benign	Het
Ighv5-9	C	T	12: 113,661,877	S82N	probably benign	Het
Il1rap	C	A	16: 26,631,593		probably benign	Het
Inhbc	N		10: 127,367,140		probably benign	Het
Inpp4b	C	T	8: 81,693,010		probably benign	Het
Kars	N		8: 111,993,708		probably benign	Het
Kcng4	G	T	8: 119,633,519	Y39*	probably null	Het
Kcnh7	A	G	2: 63,049,799		probably benign	Het
Klhl1	G	C	14: 96,317,730		probably benign	Het
Lrch1	C	T	14: 74,905,021		probably benign	Het
Lrp1b	G	C	2: 42,237,562		probably benign	Het
Lsm8	G	A	6: 18,844,316		probably benign	Het
Lsm8	G	A	6: 18,854,321		probably benign	Het
Magi2	T	C	5: 19,954,511		probably benign	Het
Marc2	TAGGCAGCCAATCAGAGCACTGCCTCTCAGCGCCAGGCAGCCAATCAGAGCACTGCCTCTCAGCGCCAGGCA	TAGGCAGCCAATCAGAGCACTGCCTCTCAGCGCCAGGCA	1: 184,822,788		probably benign	Het
Mef2c	G	A	13: 83,605,823		probably benign	Het
Mei4	A	G	9: 82,186,959		probably benign	Het
Mid1	T	A	X: 170,005,564		probably benign	Het
Mndal	G	C	1: 173,880,218		probably benign	Het
Mpped2	C	A	2: 106,721,428		probably benign	Het
Mtf1	A	G	4: 124,829,316		probably benign	Het
Mxd1	T	C	6: 86,667,406		probably benign	Het
Nbea	G	T	3: 55,701,527		probably benign	Het
Ncor1	N		11: 62,419,782		probably benign	Het
Nox4	A	G	7: 87,243,067		probably benign	Het
Ocln	C	T	13: 100,520,567		probably benign	Het
Ofcc1	G	A	13: 40,092,967		probably benign	Het
Olfr313	T	A	11: 58,817,440	V144D	possibly damaging	Het
Paics	N		5: 76,956,744		probably null	Het
Paqr8	G	T	1: 20,914,658		probably benign	Het
Pdss1	C	T	2: 22,900,968		probably benign	Het
Piezo1	G	A	8: 122,495,569	R503W	probably damaging	Het
Pkhd1	G	A	1: 20,611,414		probably benign	Het
Ppp1r3f	C	A	X: 7,560,336	G562V	probably damaging	Het
Prr16	C	T	18: 51,200,324		probably benign	Het
Prss38	T	G	11: 59,375,641		probably benign	Het
Ptprk	T	C	10: 28,574,987		probably benign	Het
Pum1	N		4: 130,730,549		probably benign	Het
Rabgef1	N		5: 130,187,586		probably null	Het
Rgs16	G	A	1: 153,744,230		probably benign	Het
Riok3	G	T	18: 12,129,982		probably benign	Het
Robo2	N		16: 74,055,954		probably benign	Het
Rps6ka3	A	G	X: 159,279,215		probably benign	Het
Rptn	T	A	3: 93,397,541	L727Q	probably benign	Het
Slc25a46	C	A	18: 31,597,313		probably benign	Het
Spsb1	C	T	4: 149,952,486		probably benign	Het
Tbc1d19	A	C	5: 53,816,709		probably benign	Het
Trav7-4	C	T	14: 53,461,518	L41F	probably benign	Het
Trip12	N		1: 84,754,482		probably benign	Het
Ttc13	G	A	8: 124,675,866		probably benign	Het
Ttll6	C	T	11: 96,155,258		probably benign	Het
Unc5b	C	G	10: 60,786,171		probably benign	Het
Vmn2r87	C	T	10: 130,478,714	M334I	probably benign	Het
Vps13b	G	T	15: 35,846,957	A2629S	probably damaging	Het
Wars2	N		3: 99,214,774		probably null	Het
Zdhhc3	AACACACACACACACACACACACACACACACAC	AACACACACACACACACACACACACACACACACAC	9: 123,083,022		probably benign	Het
Zfp26	C	A	9: 20,438,546	A241S	probably benign	Het
Zfp808	C	T	13: 62,166,651		probably benign	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150409347	nonsense	probably null
IGL02566:Zfp442	APN	2	150409791	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150409794	splice site	probably benign
PIT4812001:Zfp442	UTSW	2	150409741	nonsense	probably null
R0219:Zfp442	UTSW	2	150411240	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150411249	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150408340	nonsense	probably null
R1702:Zfp442	UTSW	2	150409180	nonsense	probably null
R1829:Zfp442	UTSW	2	150409063	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150408180	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150408662	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150408122	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150409606	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150408229	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150411210	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150409715	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150408495	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150409610	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150408159	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150408024	nonsense	probably null
R6042:Zfp442	UTSW	2	150408096	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150451401	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150408108	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150409579	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150410940	critical splice donor site	probably null
R7061:Zfp442	UTSW	2	150408017	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150408136	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150409281	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150409005	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150408756	missense	unknown
R7591:Zfp442	UTSW	2	150408172	nonsense	probably null
R7679:Zfp442	UTSW	2	150410997	nonsense	probably null
R7768:Zfp442	UTSW	2	150408321	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150409719	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150409482	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150411226	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150409176	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150408709	missense	unknown
R8528:Zfp442	UTSW	2	150409042	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150408173	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150409367	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150408756	missense	unknown
R9401:Zfp442	UTSW	2	150409695	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150408748	missense	unknown
R9711:Zfp442	UTSW	2	150408287	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150408479	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AATGGTCAGGACATGATGGC -3'
(R):5'- CCTAAGATCCTGGGTGTGCTAG -3'

Sequencing Primer
(F):5'- TGGTCAGGACATGATGGCAGAAG -3'
(R):5'- ACCATGGGACTGTCCACC -3'

Posted On

2017-05-26