Mutagenetix > Incidental Mutation

Incidental Mutation 'R6017:Olfr855'

478646

Institutional Source

Beutler Lab

Gene Symbol

Olfr855

Ensembl Gene

ENSMUSG00000043087

Gene Name

olfactory receptor 855

Synonyms

MOR148-1, GA_x6K02T2PVTD-13330461-13331399

MMRRC Submission

044191-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R6017 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19582617-19586030 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19585434 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 299 (V299A)

Ref Sequence

ENSEMBL: ENSMUSP00000150218 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061693] [ENSMUST00000215587]

AlphaFold

Q7TRF8

Predicted Effect

probably benign
Transcript: ENSMUST00000061693
AA Change: V299A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000054790
Gene: ENSMUSG00000043087
AA Change: V299A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	310	1.7e-54	PFAM
Pfam:7TM_GPCR_Srsx	37	182	5.2e-8	PFAM
Pfam:7tm_1	43	292	2.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215587
AA Change: V299A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.0%
20x: 90.5%

Validation Efficiency

97% (62/64)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9030624J02Rik	T	A	7: 118,809,921	V635D	probably damaging	Het
Adgb	T	C	10: 10,450,036	I56M	probably damaging	Het
Adgrv1	A	T	13: 81,397,423	L5581*	probably null	Het
Arpc2	C	A	1: 74,262,486	H193N	probably benign	Het
B3galt5	A	G	16: 96,315,184	T6A	probably benign	Het
Bod1l	A	T	5: 41,818,760	V1737E	probably benign	Het
Cacfd1	T	G	2: 27,013,428		probably benign	Het
Cdc42ep4	A	T	11: 113,729,366	D66E	probably benign	Het
Cldn1	G	T	16: 26,363,219	T80N	probably damaging	Het
Cmtm1	C	A	8: 104,310,951		probably benign	Het
Cntnap5c	A	T	17: 58,104,698	I526F	probably benign	Het
Copb1	T	C	7: 114,236,797	K450E	probably benign	Het
Crebrf	A	C	17: 26,757,849	I416L	probably benign	Het
Csmd3	C	A	15: 48,314,012	V377F	possibly damaging	Het
Cyp1a2	T	C	9: 57,681,030	K304E	probably damaging	Het
Cyp2d26	A	G	15: 82,790,573	S403P	possibly damaging	Het
Cyp4a12a	T	A	4: 115,326,279	C198*	probably null	Het
Ddx11	A	G	17: 66,130,017	D102G		Het
Dpys	T	A	15: 39,846,718	Q105L	probably null	Het
Dsn1	C	A	2: 156,996,242	R334L	probably damaging	Het
Eml2	G	A	7: 19,201,163	V432I	probably damaging	Het
Epha8	G	T	4: 136,931,743	H867N	probably damaging	Het
Ephb3	T	C	16: 21,222,031	L643P	probably damaging	Het
Fbxo44	G	T	4: 148,158,553	H83Q	probably benign	Het
Gm13102	T	C	4: 144,109,237	Y477H	possibly damaging	Het
Gm7347	T	C	5: 26,057,278	T82A	probably benign	Het
Gss	C	A	2: 155,587,465	A36S	probably benign	Het
Hepacam	A	G	9: 37,380,760	D128G	probably benign	Het
Hepacam2	C	A	6: 3,483,332	V226F	probably damaging	Het
Hgfac	T	C	5: 35,044,395	Y291H	probably damaging	Het
Ip6k2	G	A	9: 108,797,267	R88H	probably benign	Het
Irx5	T	A	8: 92,358,250	Y23N	probably damaging	Het
Kcnf1	T	C	12: 17,175,081	M380V	probably damaging	Het
Kcnj1	A	T	9: 32,394,104		probably benign	Het
Kcnk12	T	C	17: 87,746,736	E166G	probably damaging	Het
Kctd16	T	C	18: 40,258,943	C195R	probably damaging	Het
Kif28	T	A	1: 179,699,453	I718F	probably benign	Het
Lce1e	T	A	3: 92,707,933	K36*	probably null	Het
Map4	T	C	9: 110,034,619	L304P	probably benign	Het
Mettl17	C	T	14: 51,891,617		probably benign	Het
Mpp4	T	C	1: 59,121,359	D595G	probably damaging	Het
Myo18a	A	G	11: 77,841,523	K1282E	probably damaging	Het
Nf2	A	T	11: 4,816,137	V131D	possibly damaging	Het
Olfr147	A	T	9: 38,403,620	M249L	probably benign	Het
Oxsr1	A	G	9: 119,264,777	L270S	probably benign	Het
Plekhg2	G	A	7: 28,362,884	T536I	probably damaging	Het
Ppp1r9a	T	A	6: 4,906,363	V306D	probably benign	Het
Ptk6	C	T	2: 181,195,812	C438Y	probably benign	Het
Scfd1	T	C	12: 51,445,678	V590A	probably damaging	Het
Serpina1b	A	G	12: 103,729,272	S337P	probably damaging	Het
Skor2	T	A	18: 76,858,927	C115S	unknown	Het
Slc2a7	T	C	4: 150,165,172	S407P	probably damaging	Het
Slc8a1	A	G	17: 81,648,254	S452P	probably damaging	Het
Spata31d1c	A	G	13: 65,035,079	D145G	possibly damaging	Het
Spata6	C	A	4: 111,774,827	T145K	probably damaging	Het
Stab1	C	T	14: 31,141,544	R2087H	probably benign	Het
Stk24	C	T	14: 121,302,245	V180M	probably benign	Het
Trrap	A	G	5: 144,844,241	T3271A	probably damaging	Het
Tyro3	T	A	2: 119,816,666	W755R	probably damaging	Het
Ush2a	T	C	1: 188,957,514		probably null	Het
Uts2b	T	C	16: 27,361,043		probably null	Het
Vmn2r105	T	A	17: 20,208,627	H729L	probably damaging	Het
Wdfy3	T	C	5: 101,851,359	I3068V	probably benign	Het
Zfp457	G	A	13: 67,293,699	H175Y	probably damaging	Het
Zfp758	A	G	17: 22,373,731	D40G	probably damaging	Het

Other mutations in Olfr855

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Olfr855	APN	9	19585320	missense	possibly damaging	0.74
IGL01405:Olfr855	APN	9	19585205	missense	probably benign	0.23
IGL01775:Olfr855	APN	9	19584705	missense	probably benign	0.04
IGL01920:Olfr855	APN	9	19585022	missense	probably benign	0.01
R0501:Olfr855	UTSW	9	19584618	missense	probably damaging	1.00
R0600:Olfr855	UTSW	9	19585304	missense	possibly damaging	0.47
R0667:Olfr855	UTSW	9	19585447	missense	probably benign
R1769:Olfr855	UTSW	9	19585386	missense	probably damaging	0.98
R3117:Olfr855	UTSW	9	19584941	missense	probably damaging	0.99
R4002:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4003:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4043:Olfr855	UTSW	9	19584995	missense	probably benign	0.16
R4243:Olfr855	UTSW	9	19584558	missense	probably damaging	1.00
R4672:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4673:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4959:Olfr855	UTSW	9	19585208	missense	probably benign
R4973:Olfr855	UTSW	9	19585208	missense	probably benign
R5223:Olfr855	UTSW	9	19585026	missense	probably benign	0.16
R5681:Olfr855	UTSW	9	19584899	missense	probably damaging	1.00
R6005:Olfr855	UTSW	9	19584885	missense	probably benign	0.45
R6145:Olfr855	UTSW	9	19584888	missense	probably benign	0.02
R6615:Olfr855	UTSW	9	19584989	missense	probably benign	0.05
R6771:Olfr855	UTSW	9	19585379	missense	probably benign	0.16
R6969:Olfr855	UTSW	9	19584590	missense	possibly damaging	0.77
R7239:Olfr855	UTSW	9	19585191	missense	probably damaging	1.00
R7313:Olfr855	UTSW	9	19584642	missense	probably damaging	1.00
R7361:Olfr855	UTSW	9	19584560	missense	probably benign	0.00
R8112:Olfr855	UTSW	9	19584724	missense	probably benign	0.44
R8470:Olfr855	UTSW	9	19584969	missense	probably damaging	0.99
R9155:Olfr855	UTSW	9	19585083	missense	probably benign	0.00
R9187:Olfr855	UTSW	9	19584654	missense	probably benign	0.03
R9422:Olfr855	UTSW	9	19584672	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATAAAGCCTTCTCTACTTGCGGG -3'
(R):5'- ATGTTTCACAGATGTTGACGCATAG -3'

Sequencing Primer
(F):5'- ACTTGCGGGACTCACTTGTCAG -3'
(R):5'- TGTTGACGCATAGAGAGTCC -3'

Posted On

2017-06-26