Mutagenetix > Incidental Mutation

Incidental Mutation 'R6006:Zfp955a'

479507

Institutional Source

Beutler Lab

Gene Symbol

Zfp955a

Ensembl Gene

ENSMUSG00000094441

Gene Name

zinc finger protein 955A

Synonyms

AI842447

MMRRC Submission

044183-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R6006 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

33458692-33474119 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 33460660 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 491 (C491R)

Ref Sequence

ENSEMBL: ENSMUSP00000008830 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000008830]

AlphaFold

Q80XR7

Predicted Effect

probably damaging
Transcript: ENSMUST00000008830
AA Change: C491R

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000008830
Gene: ENSMUSG00000094441
AA Change: C491R

Domain	Start	End	E-Value	Type
KRAB	10	71	7.08e-15	SMART
ZnF_C2H2	230	252	7.29e0	SMART
ZnF_C2H2	258	280	5.72e-1	SMART
ZnF_C2H2	290	312	6.57e-1	SMART
ZnF_C2HC	291	307	9.75e0	SMART
ZnF_C2H2	318	340	7.67e-2	SMART
ZnF_C2H2	346	368	3.16e-3	SMART
ZnF_C2H2	374	396	1.18e-2	SMART
ZnF_C2H2	402	424	2.99e-4	SMART
ZnF_C2H2	430	452	2.09e-3	SMART
ZnF_C2H2	458	480	6.57e-1	SMART
ZnF_C2HC	459	475	4.03e1	SMART
ZnF_C2H2	486	508	1.28e-3	SMART
ZnF_C2H2	514	536	2.36e-2	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.3%
20x: 91.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	A	G	8: 124,694,804 (GRCm39)	I296T	probably benign	Het
Abcb4	C	A	5: 8,996,026 (GRCm39)	T894K	probably damaging	Het
Acad12	C	T	5: 121,737,299 (GRCm39)	V491I	possibly damaging	Het
Actr8	T	A	14: 29,706,099 (GRCm39)		probably null	Het
Adam7	A	C	14: 68,748,845 (GRCm39)	D467E	probably damaging	Het
Adamts18	A	T	8: 114,433,606 (GRCm39)	C1043S	probably damaging	Het
Adgrb3	T	G	1: 25,865,612 (GRCm39)	D77A	possibly damaging	Het
Arhgap20	T	A	9: 51,761,426 (GRCm39)	D1056E	probably benign	Het
Bco1	A	G	8: 117,840,330 (GRCm39)		probably null	Het
Btn2a2	A	T	13: 23,670,533 (GRCm39)	W67R	probably damaging	Het
Camkmt	A	T	17: 85,759,666 (GRCm39)	N269Y	possibly damaging	Het
Cand1	A	G	10: 119,045,933 (GRCm39)	F991L	possibly damaging	Het
Cchcr1	A	G	17: 35,835,597 (GRCm39)	K234E	possibly damaging	Het
Cd14	T	C	18: 36,859,335 (GRCm39)	D40G	possibly damaging	Het
Cdc73	A	T	1: 143,493,177 (GRCm39)	F386I	probably damaging	Het
Cdk14	C	T	5: 5,299,211 (GRCm39)	M1I	probably null	Het
Cnpy3	A	C	17: 47,047,790 (GRCm39)	S220A	probably benign	Het
Col22a1	A	G	15: 71,845,685 (GRCm39)	V359A	probably damaging	Het
Col6a3	C	A	1: 90,696,105 (GRCm39)	C2654F	unknown	Het
Colgalt2	A	G	1: 152,348,912 (GRCm39)	T186A	probably damaging	Het
Cpsf4l	A	G	11: 113,590,753 (GRCm39)	V199A	probably benign	Het
Dhtkd1	G	A	2: 5,908,836 (GRCm39)	Q753*	probably null	Het
Ep400	A	G	5: 110,852,825 (GRCm39)	S1307P	unknown	Het
Fer1l6	T	C	15: 58,518,893 (GRCm39)	V1675A	probably damaging	Het
Glis1	T	C	4: 107,425,103 (GRCm39)	L238P	probably damaging	Het
Ift70a1	A	G	2: 75,811,832 (GRCm39)	Y84H	probably benign	Het
Iqgap3	T	A	3: 87,998,854 (GRCm39)	D318E	probably damaging	Het
Lats1	T	C	10: 7,581,359 (GRCm39)	F715L	probably damaging	Het
Mlxip	T	C	5: 123,583,721 (GRCm39)	F428S	possibly damaging	Het
Morc3	T	G	16: 93,663,381 (GRCm39)	I528R	possibly damaging	Het
Mtx1	C	T	3: 89,117,613 (GRCm39)	G60D	probably damaging	Het
Mug2	G	C	6: 122,060,459 (GRCm39)	Q1398H	probably null	Het
Mup8	T	C	4: 60,220,403 (GRCm39)	I110V	probably benign	Het
Nfkb1	A	T	3: 135,309,522 (GRCm39)	L12*	probably null	Het
Numa1	G	A	7: 101,641,926 (GRCm39)		probably null	Het
Or52e18	T	G	7: 104,609,870 (GRCm39)	E23A	probably damaging	Het
Pbk	C	T	14: 66,054,094 (GRCm39)	P213L	probably damaging	Het
Pdcd1lg2	T	A	19: 29,431,905 (GRCm39)	H224Q	possibly damaging	Het
Pkp1	C	G	1: 135,805,406 (GRCm39)		probably null	Het
Rela	A	G	19: 5,689,967 (GRCm39)	N139S	probably damaging	Het
Rgs3	G	A	4: 62,542,143 (GRCm39)	R39Q	probably damaging	Het
S1pr3	A	G	13: 51,573,731 (GRCm39)	E304G	probably damaging	Het
Sertad2	G	A	11: 20,597,884 (GRCm39)	G27S	probably benign	Het
Setmar	T	C	6: 108,053,387 (GRCm39)	S294P	possibly damaging	Het
Smc2	A	G	4: 52,459,024 (GRCm39)	N473S	probably benign	Het
Ssr1	TCTCTTTC	T	13: 38,169,972 (GRCm39)		probably null	Het
Tigd2	A	G	6: 59,187,762 (GRCm39)	I210V	possibly damaging	Het
Tmprss9	T	A	10: 80,719,555 (GRCm39)	F93L	possibly damaging	Het
U2surp	T	C	9: 95,361,360 (GRCm39)	Y633C	probably damaging	Het
Usp18	A	T	6: 121,239,781 (GRCm39)	E292V	possibly damaging	Het
Usp32	A	C	11: 84,883,277 (GRCm39)		probably null	Het
Utp18	T	C	11: 93,776,449 (GRCm39)	D12G	probably benign	Het
Vmn1r216	C	A	13: 23,283,928 (GRCm39)	H204N	probably benign	Het
Wdr97	T	C	15: 76,241,372 (GRCm39)	V626A	probably damaging	Het
Wwc1	A	T	11: 35,761,809 (GRCm39)	V619E	probably null	Het
Wwc1	T	C	11: 35,780,100 (GRCm39)	D285G	probably damaging	Het
Zfp3	A	T	11: 70,662,590 (GRCm39)	Q183L	probably benign	Het

Other mutations in Zfp955a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01115:Zfp955a	APN	17	33,461,554 (GRCm39)	nonsense	probably null
IGL01859:Zfp955a	APN	17	33,462,693 (GRCm39)	missense	probably benign	0.45
IGL02612:Zfp955a	APN	17	33,463,039 (GRCm39)	missense	probably damaging	0.99
IGL02894:Zfp955a	APN	17	33,461,426 (GRCm39)	nonsense	probably null
IGL02933:Zfp955a	APN	17	33,462,683 (GRCm39)	splice site	probably null
R0145:Zfp955a	UTSW	17	33,461,430 (GRCm39)	missense	probably damaging	0.98
R0577:Zfp955a	UTSW	17	33,461,068 (GRCm39)	missense	probably damaging	0.99
R0963:Zfp955a	UTSW	17	33,462,726 (GRCm39)	missense	probably benign	0.00
R1588:Zfp955a	UTSW	17	33,460,791 (GRCm39)	missense	probably benign	0.00
R1614:Zfp955a	UTSW	17	33,461,306 (GRCm39)	missense	possibly damaging	0.72
R1704:Zfp955a	UTSW	17	33,460,699 (GRCm39)	nonsense	probably null
R1994:Zfp955a	UTSW	17	33,460,620 (GRCm39)	missense	probably damaging	0.99
R2043:Zfp955a	UTSW	17	33,461,527 (GRCm39)	missense	possibly damaging	0.94
R2091:Zfp955a	UTSW	17	33,461,731 (GRCm39)	nonsense	probably null
R2091:Zfp955a	UTSW	17	33,461,731 (GRCm39)	nonsense	probably null
R4077:Zfp955a	UTSW	17	33,460,675 (GRCm39)	missense	probably benign	0.15
R4078:Zfp955a	UTSW	17	33,460,675 (GRCm39)	missense	probably benign	0.15
R4689:Zfp955a	UTSW	17	33,461,040 (GRCm39)	missense	probably damaging	1.00
R4735:Zfp955a	UTSW	17	33,460,696 (GRCm39)	missense	probably benign	0.09
R4870:Zfp955a	UTSW	17	33,460,699 (GRCm39)	nonsense	probably null
R4904:Zfp955a	UTSW	17	33,461,162 (GRCm39)	nonsense	probably null
R5180:Zfp955a	UTSW	17	33,461,592 (GRCm39)	missense	probably benign	0.15
R7132:Zfp955a	UTSW	17	33,460,589 (GRCm39)	nonsense	probably null
R7403:Zfp955a	UTSW	17	33,462,720 (GRCm39)	missense	probably benign	0.01
R7457:Zfp955a	UTSW	17	33,463,025 (GRCm39)	nonsense	probably null
R7547:Zfp955a	UTSW	17	33,461,797 (GRCm39)	missense	probably benign	0.05
R8263:Zfp955a	UTSW	17	33,463,087 (GRCm39)	missense	probably damaging	1.00
R8265:Zfp955a	UTSW	17	33,463,087 (GRCm39)	missense	probably damaging	1.00
R8330:Zfp955a	UTSW	17	33,463,087 (GRCm39)	missense	probably damaging	1.00
R8947:Zfp955a	UTSW	17	33,460,955 (GRCm39)	missense	probably damaging	1.00
R9052:Zfp955a	UTSW	17	33,461,279 (GRCm39)	missense	possibly damaging	0.53
R9352:Zfp955a	UTSW	17	33,461,335 (GRCm39)	missense	probably benign	0.01
R9557:Zfp955a	UTSW	17	33,461,107 (GRCm39)	nonsense	probably null
R9683:Zfp955a	UTSW	17	33,461,587 (GRCm39)	missense	probably benign	0.01
R9784:Zfp955a	UTSW	17	33,461,149 (GRCm39)	missense	probably damaging	1.00
R9797:Zfp955a	UTSW	17	33,461,888 (GRCm39)	missense	possibly damaging	0.59
X0062:Zfp955a	UTSW	17	33,460,976 (GRCm39)	missense	probably benign	0.37

Predicted Primers

PCR Primer
(F):5'- AAGCATGCAAACGCTTTCCC -3'
(R):5'- GGCAACTGTTCCAACCATGAAC -3'

Sequencing Primer
(F):5'- GAATTTCTCTCTAGCACTATGAGTTC -3'
(R):5'- ACCTACGTTTGCAAGCAGTG -3'

Posted On

2017-06-26