Mutagenetix > Incidental Mutation

Incidental Mutation 'R0513:Tenm4'

48002

Institutional Source

Beutler Lab

Gene Symbol

Tenm4

Ensembl Gene

ENSMUSG00000048078

Gene Name

teneurin transmembrane protein 4

Synonyms

Doc4, l7Rn3, Ten-m4, ELM2, l(7)-3Rn, Odz4

MMRRC Submission

038707-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0513 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

96171246-96911093 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 96895623 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 2311 (M2311T)

Ref Sequence

ENSEMBL: ENSMUSP00000102780 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000107162] [ENSMUST00000107165] [ENSMUST00000107166]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000107162
AA Change: M2311T

PolyPhen 2 Score 0.349 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000102780
Gene: ENSMUSG00000048078
AA Change: M2311T

Domain	Start	End	E-Value	Type
Pfam:Ten_N	10	410	5.6e-195	PFAM
transmembrane domain	411	433	N/A	INTRINSIC
EGF_like	637	665	3.43e1	SMART
EGF	668	696	2.29e1	SMART
EGF	701	730	1.88e-1	SMART
EGF	733	762	1.13e1	SMART
EGF	767	797	2.39e1	SMART
EGF	800	828	4.32e-1	SMART
EGF	831	859	6.02e0	SMART
EGF	862	894	9.93e-1	SMART
low complexity region	900	914	N/A	INTRINSIC
Pfam:RHS_repeat	2327	2380	5.5e-7	PFAM
Pfam:Tox-GHH	2740	2818	5.2e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107165
AA Change: M2319T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102783
Gene: ENSMUSG00000048078
AA Change: M2319T

Domain	Start	End	E-Value	Type
Pfam:Ten_N	36	402	1.1e-171	PFAM
transmembrane domain	403	425	N/A	INTRINSIC
EGF_like	629	657	3.43e1	SMART
EGF	660	688	2.29e1	SMART
EGF	693	722	1.88e-1	SMART
EGF	725	754	1.13e1	SMART
EGF	759	789	2.39e1	SMART
EGF	792	820	4.32e-1	SMART
EGF	823	851	6.02e0	SMART
EGF	863	895	9.93e-1	SMART
low complexity region	901	915	N/A	INTRINSIC
Pfam:RHS_repeat	2335	2368	1.6e-7	PFAM
Pfam:Tox-GHH	2749	2826	1.8e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107166
AA Change: M2282T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000102784
Gene: ENSMUSG00000048078
AA Change: M2282T

Domain	Start	End	E-Value	Type
Pfam:Ten_N	35	193	1.4e-83	PFAM
Pfam:Ten_N	187	365	5e-78	PFAM
transmembrane domain	366	388	N/A	INTRINSIC
EGF_like	592	620	3.43e1	SMART
EGF	623	651	2.29e1	SMART
EGF	656	685	1.88e-1	SMART
EGF	688	717	1.13e1	SMART
EGF	722	752	2.39e1	SMART
EGF	755	783	4.32e-1	SMART
EGF	786	814	6.02e0	SMART
EGF	826	858	9.93e-1	SMART
low complexity region	864	878	N/A	INTRINSIC
Pfam:RHS_repeat	2298	2351	3.8e-7	PFAM
Pfam:Tox-GHH	2711	2789	3.9e-34	PFAM

Meta Mutation Damage Score

0.0844

Coding Region Coverage

1x: 99.5%
3x: 98.7%
10x: 96.6%
20x: 92.9%

Validation Efficiency

99% (122/123)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene plays a role in establishing proper neuronal connectivity during development. Defects in this gene have been associated with hereditary essential tremor-5. [provided by RefSeq, Oct 2016]
PHENOTYPE: Various ENU-induced alleles cause prenatal lethality associated with impaired mesoderm development and lead to pleiotropic phenotypes. The most severe alleles cause failure of gastrulation and somitogenesis while the least severe one allows survival to adulthood with runting of variable penetrance. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 118 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	A	G	13: 119,469,659 (GRCm38)	T146A	probably benign	Het
4930452B06Rik	A	C	14: 8,536,609 (GRCm38)	D199E	probably damaging	Het
5830473C10Rik	A	T	5: 90,577,927 (GRCm38)	T333S	probably benign	Het
6720489N17Rik	A	C	13: 62,605,215 (GRCm38)	V99G	possibly damaging	Het
Abcg4	A	G	9: 44,281,687 (GRCm38)	S121P	possibly damaging	Het
Actr2	T	C	11: 20,080,124 (GRCm38)	T212A	probably damaging	Het
Adcy10	T	A	1: 165,519,519 (GRCm38)	D368E	probably benign	Het
Anapc15	T	C	7: 101,898,540 (GRCm38)		probably benign	Het
Aox4	A	G	1: 58,217,519 (GRCm38)	R67G	probably benign	Het
Aox4	A	G	1: 58,247,300 (GRCm38)	D697G	probably damaging	Het
Arhgap42	A	T	9: 9,005,765 (GRCm38)	S755T	probably benign	Het
Atm	A	G	9: 53,503,948 (GRCm38)	V881A	probably benign	Het
Cd163l1	C	A	7: 140,224,960 (GRCm38)	C625*	probably null	Het
Cdkal1	C	T	13: 29,625,965 (GRCm38)		probably benign	Het
Cfap61	C	A	2: 146,035,295 (GRCm38)	N491K	possibly damaging	Het
Chgb	T	C	2: 132,785,977 (GRCm38)		probably benign	Het
Chrna1	C	A	2: 73,568,082 (GRCm38)		probably benign	Het
Chst10	A	G	1: 38,865,763 (GRCm38)	L283P	probably damaging	Het
Clca3a1	A	G	3: 144,760,562 (GRCm38)		probably null	Het
Cryzl1	G	T	16: 91,699,287 (GRCm38)	A1E	possibly damaging	Het
Csnk1a1	T	C	18: 61,576,547 (GRCm38)	Y213H	probably damaging	Het
Cspg4	A	G	9: 56,898,091 (GRCm38)	Q2062R	probably benign	Het
Ctnnal1	A	G	4: 56,835,348 (GRCm38)	C310R	probably benign	Het
D630003M21Rik	T	C	2: 158,200,308 (GRCm38)	E906G	probably benign	Het
D930048N14Rik	T	C	11: 51,654,928 (GRCm38)		probably benign	Het
Dag1	G	A	9: 108,208,485 (GRCm38)	P486S	possibly damaging	Het
Dgkq	A	G	5: 108,656,495 (GRCm38)	L33P	probably benign	Het
Dlc1	C	T	8: 36,584,010 (GRCm38)	G856R	probably damaging	Het
Dst	T	A	1: 34,219,531 (GRCm38)		probably benign	Het
Dtl	A	T	1: 191,569,707 (GRCm38)	Y79*	probably null	Het
Egfr	T	G	11: 16,872,855 (GRCm38)	L406R	probably damaging	Het
Elp3	A	T	14: 65,563,246 (GRCm38)		probably null	Het
Fbxw19	C	A	9: 109,481,553 (GRCm38)		probably null	Het
Frs2	T	C	10: 117,074,665 (GRCm38)	E264G	possibly damaging	Het
Fscn2	G	T	11: 120,361,880 (GRCm38)	V58L	probably damaging	Het
Gm17324	G	A	9: 78,448,725 (GRCm38)		probably benign	Het
Gm4787	A	T	12: 81,378,312 (GRCm38)	N357K	probably benign	Het
Gm9956	G	T	10: 56,745,195 (GRCm38)			Het
Gsg1l	C	T	7: 126,020,623 (GRCm38)		probably null	Het
Herc1	G	A	9: 66,445,645 (GRCm38)	V2138M	possibly damaging	Het
Htr2a	T	C	14: 74,706,324 (GRCm38)	L448P	probably benign	Het
Ing3	C	T	6: 21,970,035 (GRCm38)	S255L	probably damaging	Het
Ispd	A	T	12: 36,390,468 (GRCm38)	H125L	probably damaging	Het
Krt78	T	C	15: 101,950,949 (GRCm38)	D271G	probably damaging	Het
Lmbrd2	T	A	15: 9,194,729 (GRCm38)	L606H	probably damaging	Het
Lmod1	T	A	1: 135,325,168 (GRCm38)	N53K	probably damaging	Het
Lsr	G	C	7: 30,958,338 (GRCm38)	A467G	probably benign	Het
Mbtd1	T	A	11: 93,932,212 (GRCm38)		probably null	Het
Mill1	T	A	7: 18,264,877 (GRCm38)	Y337*	probably null	Het
Mlxipl	A	T	5: 135,137,263 (GRCm38)	Q833L	probably benign	Het
Mon2	C	T	10: 123,038,610 (GRCm38)	V278M	probably damaging	Het
Mxra8	A	C	4: 155,841,733 (GRCm38)	M180L	probably benign	Het
Myo18a	A	T	11: 77,811,594 (GRCm38)		probably benign	Het
Myo1c	T	G	11: 75,665,831 (GRCm38)		probably null	Het
Myo1g	T	C	11: 6,510,203 (GRCm38)	T782A	probably benign	Het
Ncapd3	A	G	9: 27,064,105 (GRCm38)		probably benign	Het
Neb	T	C	2: 52,308,687 (GRCm38)	D414G	probably damaging	Het
Nedd4l	T	A	18: 65,195,185 (GRCm38)		probably benign	Het
Nf2	T	C	11: 4,791,185 (GRCm38)	K343R	possibly damaging	Het
Nfasc	A	T	1: 132,603,846 (GRCm38)	D733E	possibly damaging	Het
Nolc1	G	A	19: 46,084,159 (GRCm38)	D699N	probably damaging	Het
Nrbp2	C	T	15: 76,088,976 (GRCm38)	A45T	probably benign	Het
Obscn	A	G	11: 59,061,522 (GRCm38)	V3907A	possibly damaging	Het
Olfr11	A	T	13: 21,638,949 (GRCm38)	D191E	probably benign	Het
Olfr1371	T	C	11: 52,213,749 (GRCm38)	Q80R	possibly damaging	Het
Olfr145	A	T	9: 37,898,055 (GRCm38)	Y217F	probably damaging	Het
Pank2	C	T	2: 131,282,606 (GRCm38)	T290I	probably damaging	Het
Pbx4	T	C	8: 69,864,879 (GRCm38)	V171A	probably benign	Het
Pcgf1	G	T	6: 83,080,574 (GRCm38)	V75F	probably damaging	Het
Pik3ca	T	C	3: 32,461,511 (GRCm38)	S778P	probably damaging	Het
Pld6	T	C	11: 59,785,221 (GRCm38)	I141M	probably damaging	Het
Polq	T	A	16: 37,094,502 (GRCm38)	V2508E	probably damaging	Het
Prkca	C	G	11: 108,014,376 (GRCm38)	D179H	possibly damaging	Het
Pspn	T	C	17: 56,999,720 (GRCm38)	S70G	probably damaging	Het
Ptchd4	C	T	17: 42,503,746 (GRCm38)	T846I	probably benign	Het
Reg3g	A	C	6: 78,467,844 (GRCm38)	Y50*	probably null	Het
Rev3l	C	T	10: 39,828,143 (GRCm38)	H2062Y	probably benign	Het
Rsph4a	C	T	10: 33,912,991 (GRCm38)	Q611*	probably null	Het
Scgb1b20	G	A	7: 33,373,314 (GRCm38)		probably null	Het
Sfxn5	T	C	6: 85,269,973 (GRCm38)		probably benign	Het
Sh3tc1	T	A	5: 35,700,307 (GRCm38)	Q1179L	possibly damaging	Het
Skint11	A	G	4: 114,194,565 (GRCm38)	I37V	probably benign	Het
Slc35f5	T	C	1: 125,576,169 (GRCm38)		probably benign	Het
Slc8a2	A	C	7: 16,157,339 (GRCm38)	D768A	probably damaging	Het
Slco6b1	A	G	1: 96,997,184 (GRCm38)		noncoding transcript	Het
Smurf2	T	A	11: 106,836,105 (GRCm38)	T453S	probably benign	Het
Spag16	A	G	1: 70,493,768 (GRCm38)		probably benign	Het
Spindoc	G	A	19: 7,374,144 (GRCm38)	T205I	probably benign	Het
Stab1	T	C	14: 31,148,945 (GRCm38)	I1316V	probably benign	Het
Stox2	T	C	8: 47,193,865 (GRCm38)	R187G	probably damaging	Het
Tcea2	A	C	2: 181,684,481 (GRCm38)	T93P	probably benign	Het
Tmem63a	A	G	1: 180,960,461 (GRCm38)	Q260R	probably benign	Het
Tnpo2	A	T	8: 85,053,529 (GRCm38)	H698L	probably benign	Het
Trim33	A	G	3: 103,310,384 (GRCm38)	D215G	probably damaging	Het
Ttc3	T	A	16: 94,426,212 (GRCm38)	I727N	probably damaging	Het
Ttn	T	A	2: 76,943,325 (GRCm38)	K2271N	probably damaging	Het
Ubr2	T	A	17: 46,986,779 (GRCm38)	K223*	probably null	Het
Ubr4	A	G	4: 139,416,875 (GRCm38)	M1410V	possibly damaging	Het
Ugt2b35	T	C	5: 87,003,412 (GRCm38)		probably benign	Het
Ulk4	T	A	9: 121,152,325 (GRCm38)	H880L	probably benign	Het
Unc80	G	A	1: 66,622,474 (GRCm38)	C1686Y	possibly damaging	Het
Upf2	T	A	2: 5,957,667 (GRCm38)	L60Q	unknown	Het
Usf2	A	T	7: 30,954,736 (GRCm38)		probably benign	Het
Usp21	T	A	1: 171,283,012 (GRCm38)		probably benign	Het
Usp21	T	A	1: 171,283,014 (GRCm38)		probably benign	Het
Vmn2r118	T	A	17: 55,610,970 (GRCm38)	K181*	probably null	Het
Vmn2r124	T	A	17: 18,073,729 (GRCm38)	S693T	possibly damaging	Het
Vmn2r76	C	A	7: 86,228,779 (GRCm38)	G470V	probably benign	Het
Vps13c	A	C	9: 67,930,735 (GRCm38)	I1856L	probably benign	Het
Vps50	C	T	6: 3,520,210 (GRCm38)	L119F	probably damaging	Het
Wdfy3	T	A	5: 101,890,789 (GRCm38)	S2012C	probably damaging	Het
Zfp142	A	G	1: 74,571,555 (GRCm38)	V924A	probably damaging	Het
Zfp217	C	T	2: 170,115,462 (GRCm38)	A539T	probably benign	Het
Zfp235	T	C	7: 24,142,219 (GRCm38)	S688P	probably damaging	Het
Zfp39	C	T	11: 58,889,987 (GRCm38)	V650I	probably benign	Het
Zfp82	A	T	7: 30,056,840 (GRCm38)	N272K	probably damaging	Het
Zfyve21	A	C	12: 111,823,264 (GRCm38)	D54A	possibly damaging	Het
Zfyve26	C	T	12: 79,244,484 (GRCm38)	D2116N	probably damaging	Het

Other mutations in Tenm4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00228:Tenm4	APN	7	96,868,009 (GRCm38)	missense	probably benign	0.00
IGL00468:Tenm4	APN	7	96,874,472 (GRCm38)	missense	probably damaging	0.98
IGL00519:Tenm4	APN	7	96,805,138 (GRCm38)	splice site	probably benign
IGL00979:Tenm4	APN	7	96,729,391 (GRCm38)	missense	probably damaging	0.96
IGL01401:Tenm4	APN	7	96,874,267 (GRCm38)	missense	probably damaging	1.00
IGL01459:Tenm4	APN	7	96,729,385 (GRCm38)	missense	probably damaging	1.00
IGL01519:Tenm4	APN	7	96,895,177 (GRCm38)	missense	probably damaging	1.00
IGL01545:Tenm4	APN	7	96,874,303 (GRCm38)	missense	probably benign	0.00
IGL01579:Tenm4	APN	7	96,863,502 (GRCm38)	missense	probably benign	0.00
IGL01587:Tenm4	APN	7	96,863,502 (GRCm38)	missense	probably benign	0.00
IGL01625:Tenm4	APN	7	96,885,358 (GRCm38)	missense	probably damaging	1.00
IGL01655:Tenm4	APN	7	96,553,724 (GRCm38)	missense	probably damaging	1.00
IGL01683:Tenm4	APN	7	96,885,404 (GRCm38)	missense	possibly damaging	0.84
IGL01728:Tenm4	APN	7	96,896,064 (GRCm38)	missense	probably damaging	1.00
IGL01732:Tenm4	APN	7	96,895,509 (GRCm38)	missense	probably damaging	1.00
IGL01924:Tenm4	APN	7	96,895,212 (GRCm38)	missense	probably damaging	1.00
IGL01966:Tenm4	APN	7	96,553,550 (GRCm38)	missense	probably damaging	1.00
IGL02177:Tenm4	APN	7	96,895,662 (GRCm38)	missense	probably benign	0.40
IGL02207:Tenm4	APN	7	96,874,116 (GRCm38)	missense	possibly damaging	0.85
IGL02269:Tenm4	APN	7	96,823,822 (GRCm38)	missense	probably damaging	1.00
IGL02274:Tenm4	APN	7	96,854,734 (GRCm38)	missense	probably damaging	1.00
IGL02375:Tenm4	APN	7	96,704,137 (GRCm38)	missense	possibly damaging	0.52
IGL02415:Tenm4	APN	7	96,874,074 (GRCm38)	missense	probably damaging	0.98
IGL02472:Tenm4	APN	7	96,774,176 (GRCm38)	unclassified	probably benign
IGL02656:Tenm4	APN	7	96,885,433 (GRCm38)	missense	probably damaging	1.00
IGL02678:Tenm4	APN	7	96,896,219 (GRCm38)	missense	probably damaging	1.00
IGL02829:Tenm4	APN	7	96,894,998 (GRCm38)	nonsense	probably null
IGL02863:Tenm4	APN	7	96,873,706 (GRCm38)	missense	probably damaging	1.00
IGL03145:Tenm4	APN	7	96,842,968 (GRCm38)	missense	probably damaging	0.98
IGL03153:Tenm4	APN	7	96,873,762 (GRCm38)	missense	probably damaging	1.00
principium	UTSW	7	96,797,481 (GRCm38)	missense	probably damaging	0.98
toccata	UTSW	7	96,902,989 (GRCm38)	critical splice donor site	probably null
P0026:Tenm4	UTSW	7	96,874,527 (GRCm38)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,892,926 (GRCm38)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,892,926 (GRCm38)	missense	probably damaging	1.00
R0140:Tenm4	UTSW	7	96,896,052 (GRCm38)	missense	possibly damaging	0.78
R0164:Tenm4	UTSW	7	96,729,340 (GRCm38)	splice site	probably benign
R0277:Tenm4	UTSW	7	96,694,950 (GRCm38)	missense	possibly damaging	0.54
R0323:Tenm4	UTSW	7	96,694,950 (GRCm38)	missense	possibly damaging	0.54
R0362:Tenm4	UTSW	7	96,772,035 (GRCm38)	nonsense	probably null
R0381:Tenm4	UTSW	7	96,905,881 (GRCm38)	missense	probably damaging	1.00
R0420:Tenm4	UTSW	7	96,873,766 (GRCm38)	missense	possibly damaging	0.85
R0426:Tenm4	UTSW	7	96,777,851 (GRCm38)	missense	probably damaging	1.00
R0624:Tenm4	UTSW	7	96,774,020 (GRCm38)	missense	probably damaging	1.00
R0837:Tenm4	UTSW	7	96,896,275 (GRCm38)	splice site	probably benign
R1037:Tenm4	UTSW	7	96,797,481 (GRCm38)	missense	probably damaging	0.98
R1172:Tenm4	UTSW	7	96,848,044 (GRCm38)	missense	probably damaging	1.00
R1422:Tenm4	UTSW	7	96,550,051 (GRCm38)	missense	probably damaging	0.99
R1427:Tenm4	UTSW	7	96,843,048 (GRCm38)	missense	probably benign	0.42
R1462:Tenm4	UTSW	7	96,704,153 (GRCm38)	missense	probably damaging	1.00
R1462:Tenm4	UTSW	7	96,704,153 (GRCm38)	missense	probably damaging	1.00
R1597:Tenm4	UTSW	7	96,902,989 (GRCm38)	critical splice donor site	probably null
R1701:Tenm4	UTSW	7	96,902,889 (GRCm38)	missense	probably damaging	1.00
R1707:Tenm4	UTSW	7	96,888,685 (GRCm38)	missense	probably damaging	1.00
R1809:Tenm4	UTSW	7	96,873,780 (GRCm38)	missense	probably benign	0.17
R1812:Tenm4	UTSW	7	96,895,940 (GRCm38)	missense	probably damaging	1.00
R1895:Tenm4	UTSW	7	96,735,808 (GRCm38)	missense	probably damaging	1.00
R1933:Tenm4	UTSW	7	96,895,326 (GRCm38)	missense	probably damaging	1.00
R1946:Tenm4	UTSW	7	96,735,808 (GRCm38)	missense	probably damaging	1.00
R2108:Tenm4	UTSW	7	96,906,290 (GRCm38)	missense	probably damaging	1.00
R2151:Tenm4	UTSW	7	96,902,847 (GRCm38)	missense	probably damaging	1.00
R2247:Tenm4	UTSW	7	96,906,009 (GRCm38)	missense	probably benign	0.03
R2329:Tenm4	UTSW	7	96,895,862 (GRCm38)	missense	probably benign	0.00
R2893:Tenm4	UTSW	7	96,894,990 (GRCm38)	missense	probably damaging	1.00
R2990:Tenm4	UTSW	7	96,893,125 (GRCm38)	splice site	probably null
R3409:Tenm4	UTSW	7	96,895,160 (GRCm38)	missense	probably damaging	1.00
R3410:Tenm4	UTSW	7	96,852,530 (GRCm38)	missense	probably damaging	0.99
R3411:Tenm4	UTSW	7	96,852,530 (GRCm38)	missense	probably damaging	0.99
R3440:Tenm4	UTSW	7	96,553,516 (GRCm38)	missense	probably benign	0.00
R3441:Tenm4	UTSW	7	96,553,516 (GRCm38)	missense	probably benign	0.00
R3719:Tenm4	UTSW	7	96,863,563 (GRCm38)	missense	possibly damaging	0.92
R3772:Tenm4	UTSW	7	96,694,880 (GRCm38)	missense	probably damaging	1.00
R3773:Tenm4	UTSW	7	96,694,880 (GRCm38)	missense	probably damaging	1.00
R4093:Tenm4	UTSW	7	96,895,772 (GRCm38)	missense	probably damaging	1.00
R4439:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4441:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4510:Tenm4	UTSW	7	96,894,863 (GRCm38)	missense	probably benign
R4511:Tenm4	UTSW	7	96,894,863 (GRCm38)	missense	probably benign
R4543:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4645:Tenm4	UTSW	7	96,895,742 (GRCm38)	missense	probably damaging	1.00
R4701:Tenm4	UTSW	7	96,895,349 (GRCm38)	missense	probably damaging	1.00
R4707:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4714:Tenm4	UTSW	7	96,894,924 (GRCm38)	missense	probably damaging	1.00
R4742:Tenm4	UTSW	7	96,797,484 (GRCm38)	missense	probably damaging	0.99
R4784:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4785:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4801:Tenm4	UTSW	7	96,906,245 (GRCm38)	missense	probably damaging	0.97
R4802:Tenm4	UTSW	7	96,906,245 (GRCm38)	missense	probably damaging	0.97
R4880:Tenm4	UTSW	7	96,905,818 (GRCm38)	splice site	probably null
R5036:Tenm4	UTSW	7	96,852,561 (GRCm38)	missense	probably damaging	1.00
R5036:Tenm4	UTSW	7	96,694,790 (GRCm38)	missense	probably damaging	1.00
R5050:Tenm4	UTSW	7	96,895,788 (GRCm38)	missense	probably damaging	1.00
R5103:Tenm4	UTSW	7	96,842,957 (GRCm38)	missense	probably damaging	1.00
R5106:Tenm4	UTSW	7	96,843,149 (GRCm38)	missense	probably damaging	0.99
R5118:Tenm4	UTSW	7	96,893,086 (GRCm38)	missense	probably damaging	1.00
R5272:Tenm4	UTSW	7	96,874,203 (GRCm38)	missense	probably damaging	0.98
R5282:Tenm4	UTSW	7	96,837,331 (GRCm38)	missense	possibly damaging	0.90
R5403:Tenm4	UTSW	7	96,888,827 (GRCm38)	missense	probably damaging	1.00
R5404:Tenm4	UTSW	7	96,894,680 (GRCm38)	missense	probably damaging	1.00
R5567:Tenm4	UTSW	7	96,896,209 (GRCm38)	nonsense	probably null
R5590:Tenm4	UTSW	7	96,797,401 (GRCm38)	missense	possibly damaging	0.93
R5590:Tenm4	UTSW	7	96,797,400 (GRCm38)	missense	possibly damaging	0.73
R5597:Tenm4	UTSW	7	96,553,517 (GRCm38)	missense	probably benign	0.00
R5782:Tenm4	UTSW	7	96,893,039 (GRCm38)	missense	probably benign	0.00
R5861:Tenm4	UTSW	7	96,843,217 (GRCm38)	intron	probably benign
R5890:Tenm4	UTSW	7	96,902,860 (GRCm38)	missense	probably damaging	1.00
R5930:Tenm4	UTSW	7	96,854,719 (GRCm38)	missense	probably damaging	1.00
R5940:Tenm4	UTSW	7	96,845,895 (GRCm38)	missense	probably damaging	1.00
R6012:Tenm4	UTSW	7	96,522,433 (GRCm38)	intron	probably benign
R6060:Tenm4	UTSW	7	96,873,711 (GRCm38)	missense	probably damaging	1.00
R6104:Tenm4	UTSW	7	96,837,289 (GRCm38)	missense	probably damaging	0.97
R6283:Tenm4	UTSW	7	96,874,494 (GRCm38)	missense	probably benign	0.33
R6333:Tenm4	UTSW	7	96,774,124 (GRCm38)	missense	probably damaging	1.00
R6522:Tenm4	UTSW	7	96,843,044 (GRCm38)	missense	possibly damaging	0.88
R6616:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6746:Tenm4	UTSW	7	96,892,860 (GRCm38)	missense	probably damaging	1.00
R6751:Tenm4	UTSW	7	96,845,712 (GRCm38)	missense	possibly damaging	0.95
R6806:Tenm4	UTSW	7	96,811,959 (GRCm38)	missense	possibly damaging	0.95
R6807:Tenm4	UTSW	7	96,895,271 (GRCm38)	missense	probably damaging	1.00
R6807:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6809:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6810:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6811:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6853:Tenm4	UTSW	7	96,837,295 (GRCm38)	missense	possibly damaging	0.94
R6886:Tenm4	UTSW	7	96,797,392 (GRCm38)	missense	possibly damaging	0.85
R6920:Tenm4	UTSW	7	96,895,550 (GRCm38)	missense	probably damaging	1.00
R6937:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6939:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7011:Tenm4	UTSW	7	96,896,135 (GRCm38)	nonsense	probably null
R7033:Tenm4	UTSW	7	96,895,223 (GRCm38)	nonsense	probably null
R7040:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7083:Tenm4	UTSW	7	96,895,349 (GRCm38)	missense	probably damaging	1.00
R7238:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7239:Tenm4	UTSW	7	96,735,813 (GRCm38)	missense	possibly damaging	0.47
R7239:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7337:Tenm4	UTSW	7	96,874,126 (GRCm38)	missense	probably benign	0.44
R7400:Tenm4	UTSW	7	96,694,803 (GRCm38)	missense	probably damaging	0.97
R7407:Tenm4	UTSW	7	96,773,987 (GRCm38)	missense	possibly damaging	0.89
R7449:Tenm4	UTSW	7	96,874,213 (GRCm38)	missense	possibly damaging	0.65
R7473:Tenm4	UTSW	7	96,774,146 (GRCm38)	missense	probably damaging	1.00
R7477:Tenm4	UTSW	7	96,845,808 (GRCm38)	missense	probably damaging	0.99
R7489:Tenm4	UTSW	7	96,837,314 (GRCm38)	missense	possibly damaging	0.90
R7498:Tenm4	UTSW	7	96,848,017 (GRCm38)	missense	probably damaging	1.00
R7562:Tenm4	UTSW	7	96,888,814 (GRCm38)	missense	probably damaging	1.00
R7615:Tenm4	UTSW	7	96,845,926 (GRCm38)	missense	probably damaging	1.00
R7624:Tenm4	UTSW	7	96,895,985 (GRCm38)	missense	possibly damaging	0.95
R7626:Tenm4	UTSW	7	96,893,014 (GRCm38)	missense	probably damaging	1.00
R7690:Tenm4	UTSW	7	96,863,533 (GRCm38)	missense	probably benign	0.00
R7692:Tenm4	UTSW	7	96,895,403 (GRCm38)	missense	probably damaging	1.00
R7748:Tenm4	UTSW	7	96,894,702 (GRCm38)	missense	probably damaging	1.00
R7763:Tenm4	UTSW	7	96,895,692 (GRCm38)	missense	probably benign	0.38
R7792:Tenm4	UTSW	7	96,774,014 (GRCm38)	missense	possibly damaging	0.54
R7855:Tenm4	UTSW	7	96,873,874 (GRCm38)	missense	probably damaging	1.00
R7868:Tenm4	UTSW	7	96,906,380 (GRCm38)	missense	possibly damaging	0.79
R7878:Tenm4	UTSW	7	96,852,357 (GRCm38)	missense	probably damaging	1.00
R7997:Tenm4	UTSW	7	96,874,305 (GRCm38)	missense	probably benign	0.44
R8017:Tenm4	UTSW	7	96,704,041 (GRCm38)	missense	probably damaging	1.00
R8019:Tenm4	UTSW	7	96,704,041 (GRCm38)	missense	probably damaging	1.00
R8054:Tenm4	UTSW	7	96,729,346 (GRCm38)	splice site	probably benign
R8061:Tenm4	UTSW	7	96,852,456 (GRCm38)	missense	probably damaging	1.00
R8108:Tenm4	UTSW	7	96,854,728 (GRCm38)	missense	probably benign	0.39
R8140:Tenm4	UTSW	7	96,895,176 (GRCm38)	missense	probably damaging	1.00
R8214:Tenm4	UTSW	7	96,895,407 (GRCm38)	missense	probably damaging	1.00
R8258:Tenm4	UTSW	7	96,867,991 (GRCm38)	missense	probably damaging	1.00
R8259:Tenm4	UTSW	7	96,867,991 (GRCm38)	missense	probably damaging	1.00
R8364:Tenm4	UTSW	7	96,772,106 (GRCm38)	critical splice donor site	probably null
R8542:Tenm4	UTSW	7	96,811,932 (GRCm38)	missense	probably damaging	0.99
R8669:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8670:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8683:Tenm4	UTSW	7	96,902,857 (GRCm38)	missense	probably damaging	0.99
R8691:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8692:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8714:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8716:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8735:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8736:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8737:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8738:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8738:Tenm4	UTSW	7	96,873,840 (GRCm38)	missense	probably damaging	1.00
R8739:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8776:Tenm4	UTSW	7	96,895,032 (GRCm38)	missense	probably damaging	1.00
R8776-TAIL:Tenm4	UTSW	7	96,895,032 (GRCm38)	missense	probably damaging	1.00
R8777:Tenm4	UTSW	7	96,896,037 (GRCm38)	missense	probably damaging	1.00
R8777-TAIL:Tenm4	UTSW	7	96,896,037 (GRCm38)	missense	probably damaging	1.00
R8817:Tenm4	UTSW	7	96,874,128 (GRCm38)	missense	probably benign	0.01
R8851:Tenm4	UTSW	7	96,852,503 (GRCm38)	missense	probably damaging	1.00
R8913:Tenm4	UTSW	7	96,702,745 (GRCm38)	splice site	probably benign
R8977:Tenm4	UTSW	7	96,811,970 (GRCm38)	missense	probably damaging	1.00
R9100:Tenm4	UTSW	7	96,845,854 (GRCm38)	missense	probably damaging	1.00
R9136:Tenm4	UTSW	7	96,823,918 (GRCm38)	missense	possibly damaging	0.69
R9163:Tenm4	UTSW	7	96,823,873 (GRCm38)	missense	probably damaging	1.00
R9188:Tenm4	UTSW	7	96,772,027 (GRCm38)	missense	probably damaging	1.00
R9195:Tenm4	UTSW	7	96,892,919 (GRCm38)	missense	probably damaging	1.00
R9217:Tenm4	UTSW	7	96,885,439 (GRCm38)	missense	probably damaging	1.00
R9344:Tenm4	UTSW	7	96,896,145 (GRCm38)	missense	probably damaging	1.00
R9414:Tenm4	UTSW	7	96,896,160 (GRCm38)	missense	probably benign
R9466:Tenm4	UTSW	7	96,550,045 (GRCm38)	missense	possibly damaging	0.79
R9559:Tenm4	UTSW	7	96,823,849 (GRCm38)	missense	probably benign
R9626:Tenm4	UTSW	7	96,896,138 (GRCm38)	missense	probably damaging	1.00
R9673:Tenm4	UTSW	7	96,867,989 (GRCm38)	missense	probably damaging	1.00
R9676:Tenm4	UTSW	7	96,895,431 (GRCm38)	missense	probably damaging	1.00
R9678:Tenm4	UTSW	7	96,737,412 (GRCm38)	missense	possibly damaging	0.94
R9775:Tenm4	UTSW	7	96,906,554 (GRCm38)	missense	possibly damaging	0.92
R9790:Tenm4	UTSW	7	96,888,839 (GRCm38)	missense	probably damaging	1.00
R9791:Tenm4	UTSW	7	96,888,839 (GRCm38)	missense	probably damaging	1.00
R9803:Tenm4	UTSW	7	96,553,478 (GRCm38)	missense	probably damaging	1.00
X0021:Tenm4	UTSW	7	96,873,909 (GRCm38)	nonsense	probably null
X0026:Tenm4	UTSW	7	96,868,087 (GRCm38)	missense	probably damaging	0.98
X0066:Tenm4	UTSW	7	96,894,794 (GRCm38)	missense	probably damaging	1.00
X0066:Tenm4	UTSW	7	96,848,030 (GRCm38)	missense	probably damaging	1.00
Z1176:Tenm4	UTSW	7	96,905,914 (GRCm38)	missense	probably benign	0.00
Z1177:Tenm4	UTSW	7	96,863,585 (GRCm38)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- CATCAATGACAAGCCACTCTGGCG -3'
(R):5'- AGTTCCACTGAAGACAGCAAGCG -3'

Sequencing Primer
(F):5'- GCTACAGCTATGACCTCAATGG -3'
(R):5'- CCGATGTTGTCACAAGCTATG -3'

Posted On

2013-06-12