Mutagenetix > Incidental Mutation

Incidental Mutation 'R6029:Or1e16'

480280

Institutional Source

Beutler Lab

Gene Symbol

Or1e16

Ensembl Gene

ENSMUSG00000069823

Gene Name

olfactory receptor family 1 subfamily E member 16

Synonyms

GA_x6K02T2P1NL-3556334-3555390, MOR135-13, I54, Olfr1

MMRRC Submission

044201-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.196) question?

Stock #

R6029 (G1)

Quality Score

217.468

Status

Validated

Chromosome

Chromosomal Location

73285902-73290321 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

AGCGGTCGTAGGC to AGC at 73286480 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000120899 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000120303] [ENSMUST00000131253] [ENSMUST00000134011]

AlphaFold

Q8VGI1

Predicted Effect

probably null
Transcript: ENSMUST00000120303

SMART Domains

Protein: ENSMUSP00000113707
Gene: ENSMUSG00000069823

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.7e-60	PFAM
Pfam:7tm_1	41	290	2e-27	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000131253

SMART Domains

Protein: ENSMUSP00000120899
Gene: ENSMUSG00000069823

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	31	184	1.2e-6	PFAM
Pfam:7TM_GPCR_Srsx	35	171	6.1e-8	PFAM
Pfam:7tm_1	41	191	3.6e-30	PFAM
Pfam:7tm_4	139	196	1.4e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000134011

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.7%

Validation Efficiency

97% (87/90)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	G	A	2: 68,524,370 (GRCm39)		probably null	Het
Adi1	G	A	12: 28,729,318 (GRCm39)		probably benign	Het
Afg3l2	G	T	18: 67,554,329 (GRCm39)	L458M	probably damaging	Het
Apob	A	G	12: 8,066,243 (GRCm39)	E4371G	probably damaging	Het
Ash1l	T	C	3: 88,892,326 (GRCm39)	Y1402H	probably damaging	Het
Aspm	T	G	1: 139,408,728 (GRCm39)	C2538W	possibly damaging	Het
Brd10	A	G	19: 29,732,367 (GRCm39)		probably benign	Het
Btbd16	T	C	7: 130,420,802 (GRCm39)	V353A	probably benign	Het
Cad	C	T	5: 31,212,327 (GRCm39)	S10L	possibly damaging	Het
Cdc42bpa	T	C	1: 179,939,352 (GRCm39)	S877P	probably damaging	Het
Cep152	A	T	2: 125,405,552 (GRCm39)	V1660D	probably benign	Het
Clrn2	G	T	5: 45,617,528 (GRCm39)	G133V	probably damaging	Het
Clstn2	T	C	9: 97,338,634 (GRCm39)	I842V	probably benign	Het
Cpt1c	C	T	7: 44,614,548 (GRCm39)	A434T	probably benign	Het
D130052B06Rik	G	T	11: 33,573,477 (GRCm39)	V70L	possibly damaging	Het
Dlg1	T	A	16: 31,612,388 (GRCm39)	V317E	probably damaging	Het
Dock10	T	C	1: 80,514,663 (GRCm39)	E1084G	possibly damaging	Het
Dync2h1	A	G	9: 7,157,646 (GRCm39)	V827A	probably benign	Het
E030030I06Rik	A	G	10: 22,024,832 (GRCm39)	L27P	unknown	Het
Ero1b	T	A	13: 12,589,714 (GRCm39)	L39Q	probably damaging	Het
Erp27	T	C	6: 136,888,609 (GRCm39)	D123G	probably damaging	Het
Fasn	G	T	11: 120,711,735 (GRCm39)	F148L	probably damaging	Het
Fbxl5	T	C	5: 43,922,746 (GRCm39)	E218G	probably damaging	Het
Fbxw28	A	T	9: 109,158,493 (GRCm39)	D210E	probably damaging	Het
Gm10653	T	A	9: 62,748,796 (GRCm39)		probably benign	Het
Gm17067	A	G	7: 42,357,554 (GRCm39)	L316S	probably benign	Het
Gm29106	T	C	1: 118,127,990 (GRCm39)	S561P	probably damaging	Het
Hmcn1	C	A	1: 150,508,188 (GRCm39)	K3699N	probably benign	Het
Itch	T	A	2: 155,021,009 (GRCm39)		probably null	Het
Itpr3	C	T	17: 27,317,145 (GRCm39)	A800V	probably damaging	Het
Kdm2b	A	T	5: 123,017,650 (GRCm39)	M1052K	probably damaging	Het
Kdm4b	T	C	17: 56,703,576 (GRCm39)	M712T	probably damaging	Het
Kmt5b	A	G	19: 3,852,104 (GRCm39)	E137G	probably damaging	Het
Krt33a	G	A	11: 99,903,289 (GRCm39)	T251I	probably benign	Het
Lrrc4b	G	T	7: 44,111,754 (GRCm39)	R542L	probably benign	Het
Macf1	C	A	4: 123,401,126 (GRCm39)	S653I	probably damaging	Het
Macrod2	G	A	2: 142,160,367 (GRCm39)	V408M	probably damaging	Het
Macroh2a2	T	C	10: 61,583,541 (GRCm39)	T200A	possibly damaging	Het
Mapkapk3	G	T	9: 107,166,425 (GRCm39)	A40E	possibly damaging	Het
Mmp20	G	A	9: 7,639,302 (GRCm39)	V157I	probably benign	Het
Mrps28	T	C	3: 8,988,805 (GRCm39)	R18G	possibly damaging	Het
Msrb2	T	A	2: 19,399,122 (GRCm39)	C162S	probably damaging	Het
Naa80	A	G	9: 107,460,753 (GRCm39)	Y216C	probably damaging	Het
Nckap5	T	G	1: 125,953,523 (GRCm39)	T1078P	possibly damaging	Het
Nectin3	T	C	16: 46,256,763 (GRCm39)	Y91C	probably benign	Het
Nf2	G	T	11: 4,734,566 (GRCm39)		probably null	Het
Or10d1b	G	A	9: 39,613,696 (GRCm39)	A123V	probably damaging	Het
Or13a23-ps1	A	T	7: 140,118,789 (GRCm39)	M120L	unknown	Het
Or2y10	A	T	11: 49,455,428 (GRCm39)	I227F	possibly damaging	Het
Or4c29	T	C	2: 88,740,380 (GRCm39)	D119G	probably damaging	Het
Or4g7	T	A	2: 111,309,310 (GRCm39)	Y60*	probably null	Het
Or8b36	ATTGCTGTTT	ATTGCTGTTTGCTGTTT	9: 37,937,836 (GRCm39)		probably null	Het
Oxr1	T	G	15: 41,689,297 (GRCm39)	L507W	probably damaging	Het
Plxna2	A	T	1: 194,481,883 (GRCm39)	K1451M	probably damaging	Het
Plxna2	A	G	1: 194,476,735 (GRCm39)	M1184V	probably benign	Het
Pofut1	G	A	2: 153,101,713 (GRCm39)		probably null	Het
Ppp1r16b	T	C	2: 158,597,137 (GRCm39)	V257A	possibly damaging	Het
Prss56	G	A	1: 87,115,279 (GRCm39)	W498*	probably null	Het
Ptpru	A	G	4: 131,498,604 (GRCm39)	F1329S	probably damaging	Het
Pusl1	A	G	4: 155,973,920 (GRCm39)	F278S	probably damaging	Het
Qars1	A	T	9: 108,390,889 (GRCm39)	L470F	probably damaging	Het
Rbm8a2	T	C	1: 175,806,312 (GRCm39)	D55G	probably benign	Het
Rora	T	A	9: 69,271,734 (GRCm39)	N181K	probably benign	Het
Rufy3	A	G	5: 88,775,114 (GRCm39)	D262G	probably damaging	Het
Sh3gl2	G	A	4: 85,299,651 (GRCm39)	V212M	probably damaging	Het
Sis	G	A	3: 72,835,641 (GRCm39)	T907I	probably benign	Het
Slc38a3	A	T	9: 107,529,374 (GRCm39)	I456N	probably damaging	Het
Slc4a8	T	A	15: 100,705,220 (GRCm39)	C809S	probably benign	Het
Snap91	A	T	9: 86,707,133 (GRCm39)		probably null	Het
Spaca6	A	G	17: 18,051,458 (GRCm39)	T45A	probably benign	Het
Speer1m	A	T	5: 11,970,680 (GRCm39)	L84F	probably damaging	Het
Spry1	T	C	3: 37,696,997 (GRCm39)	I80T	possibly damaging	Het
St8sia2	A	G	7: 73,610,458 (GRCm39)	L275P	possibly damaging	Het
Supt7l	C	A	5: 31,684,331 (GRCm39)		probably null	Het
Tdo2	T	A	3: 81,868,747 (GRCm39)	K304N	probably damaging	Het
Tdrd12	A	T	7: 35,184,655 (GRCm39)	Y753N	probably damaging	Het
Topbp1	A	T	9: 103,222,152 (GRCm39)	Q1341L	probably benign	Het
Trim14	G	T	4: 46,506,998 (GRCm39)	A406E	probably benign	Het
Trp63	C	T	16: 25,686,964 (GRCm39)	R393W	probably damaging	Het
Trrap	T	C	5: 144,754,489 (GRCm39)	V1932A	possibly damaging	Het
Trrap	T	C	5: 144,762,724 (GRCm39)	M2399T	possibly damaging	Het
Unc45b	A	G	11: 82,804,153 (GRCm39)	N110S	probably damaging	Het
Usp32	G	T	11: 84,916,408 (GRCm39)	H845Q	probably damaging	Het
Usp44	G	A	10: 93,682,494 (GRCm39)	D268N	probably damaging	Het
Vmn1r18	T	G	6: 57,367,451 (GRCm39)	R34S	possibly damaging	Het
Vmn2r103	T	A	17: 20,014,478 (GRCm39)	Y423*	probably null	Het
Wapl	A	T	14: 34,461,204 (GRCm39)	E1054V	possibly damaging	Het
Zbtb8b	A	G	4: 129,322,286 (GRCm39)	Y392H	probably damaging	Het
Zdhhc2	A	T	8: 40,925,968 (GRCm39)	Q321L	probably null	Het
Zfp983	A	G	17: 21,881,401 (GRCm39)	D443G	probably benign	Het

Other mutations in Or1e16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01380:Or1e16	APN	11	73,286,017 (GRCm39)	missense	probably damaging	0.98
IGL01938:Or1e16	APN	11	73,286,471 (GRCm39)	missense	probably damaging	1.00
IGL02270:Or1e16	APN	11	73,286,191 (GRCm39)	missense	probably benign
IGL03287:Or1e16	APN	11	73,286,845 (GRCm39)	start codon destroyed	probably null	1.00
R0006:Or1e16	UTSW	11	73,286,314 (GRCm39)	missense	probably damaging	0.99
R0907:Or1e16	UTSW	11	73,285,945 (GRCm39)	missense	probably damaging	0.97
R1982:Or1e16	UTSW	11	73,285,918 (GRCm39)	missense	probably benign	0.00
R3804:Or1e16	UTSW	11	73,286,776 (GRCm39)	missense	probably benign	0.01
R4064:Or1e16	UTSW	11	73,286,348 (GRCm39)	missense	probably benign	0.04
R4171:Or1e16	UTSW	11	73,286,365 (GRCm39)	missense	probably damaging	1.00
R4724:Or1e16	UTSW	11	73,285,981 (GRCm39)	missense	probably damaging	1.00
R4732:Or1e16	UTSW	11	73,286,521 (GRCm39)	missense	probably benign	0.03
R4733:Or1e16	UTSW	11	73,286,521 (GRCm39)	missense	probably benign	0.03
R5030:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5097:Or1e16	UTSW	11	73,286,119 (GRCm39)	missense	probably damaging	1.00
R5098:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5101:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5135:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5137:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5192:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5193:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5193:Or1e16	UTSW	11	73,286,479 (GRCm39)	frame shift	probably null
R5197:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5220:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5221:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5222:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5258:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5297:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5396:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5398:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5399:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5432:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5433:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5531:Or1e16	UTSW	11	73,286,003 (GRCm39)	missense	probably benign	0.26
R5634:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5714:Or1e16	UTSW	11	73,286,187 (GRCm39)	splice site	probably null
R5812:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5813:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5814:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5815:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5913:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5955:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5956:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R5968:Or1e16	UTSW	11	73,286,018 (GRCm39)	missense	possibly damaging	0.75
R6034:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R6034:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R6176:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R6177:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R6178:Or1e16	UTSW	11	73,286,480 (GRCm39)	frame shift	probably null
R6196:Or1e16	UTSW	11	73,286,299 (GRCm39)	missense	probably benign	0.08
R6995:Or1e16	UTSW	11	73,286,410 (GRCm39)	missense	probably benign
R7035:Or1e16	UTSW	11	73,286,544 (GRCm39)	missense	probably benign	0.00
R7470:Or1e16	UTSW	11	73,286,714 (GRCm39)	missense	probably damaging	1.00
R7530:Or1e16	UTSW	11	73,279,189 (GRCm39)	missense	possibly damaging	0.55
R8461:Or1e16	UTSW	11	73,285,982 (GRCm39)	missense	probably damaging	1.00
R9149:Or1e16	UTSW	11	73,286,853 (GRCm39)	unclassified	probably benign
R9279:Or1e16	UTSW	11	73,279,789 (GRCm39)	missense	probably benign	0.05
R9293:Or1e16	UTSW	11	73,285,955 (GRCm39)	missense	probably damaging	0.99
R9682:Or1e16	UTSW	11	73,286,025 (GRCm39)	missense	probably benign	0.03
R9752:Or1e16	UTSW	11	73,286,479 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- TAACACGGGTGTCAGAGCAG -3'
(R):5'- TCCACACACCCATGTACTTG -3'

Sequencing Primer
(F):5'- TGTCAGAGCAGGCCAGCTTTAG -3'
(R):5'- ATGTACTTGTTTCTCAGCAACTTG -3'

Posted On

2017-06-26