Mutagenetix > Incidental Mutation

Incidental Mutation 'R5997:Adgrf3'

480608

Institutional Source

Beutler Lab

Gene Symbol

Adgrf3

Ensembl Gene

ENSMUSG00000067642

Gene Name

adhesion G protein-coupled receptor F3

Synonyms

Gpr113, LOC381628, PGR23

MMRRC Submission

044176-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5997 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

30193431-30205722 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to A at 30198362 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000085440 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088117] [ENSMUST00000125367]

AlphaFold

Q58Y75

Predicted Effect

probably null
Transcript: ENSMUST00000088117

SMART Domains

Protein: ENSMUSP00000085440
Gene: ENSMUSG00000067642

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	85	97	N/A	INTRINSIC
Blast:IG	163	252	2e-20	BLAST
Blast:CCP	341	399	1e-6	BLAST
low complexity region	403	415	N/A	INTRINSIC
low complexity region	471	483	N/A	INTRINSIC
GPS	632	684	2.68e-17	SMART
Pfam:7tm_2	687	935	1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000125367

SMART Domains

Protein: ENSMUSP00000120958
Gene: ENSMUSG00000067642

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135322

Meta Mutation Damage Score

0.9481

Coding Region Coverage

1x: 99.8%
3x: 99.4%
10x: 97.1%
20x: 90.5%

Validation Efficiency

100% (78/78)

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700061G19Rik	A	T	17: 56,876,373 (GRCm38)	D38V	probably benign	Het
Abcb9	A	T	5: 124,089,815 (GRCm38)	V121E	possibly damaging	Het
Adamts20	T	A	15: 94,379,747 (GRCm38)	Y278F	probably damaging	Het
Adcy7	A	T	8: 88,326,392 (GRCm38)	D972V	probably benign	Het
Ahdc1	G	T	4: 133,063,895 (GRCm38)	G816C	probably benign	Het
Aifm3	A	G	16: 17,502,130 (GRCm38)	K283E	probably benign	Het
Akap6	T	C	12: 52,937,233 (GRCm38)		probably null	Het
Ank1	T	C	8: 23,099,662 (GRCm38)	L593P	probably damaging	Het
Apol11b	T	A	15: 77,635,497 (GRCm38)	T128S	probably benign	Het
C1qtnf7	C	A	5: 43,616,085 (GRCm38)	T235K	probably damaging	Het
Camk2a	A	T	18: 60,977,957 (GRCm38)	I73F	probably damaging	Het
Cd109	G	T	9: 78,705,062 (GRCm38)	V1244F	possibly damaging	Het
Cep164	A	G	9: 45,769,463 (GRCm38)	L1240S	possibly damaging	Het
Cnga1	T	A	5: 72,604,575 (GRCm38)	D532V	probably damaging	Het
Cyp4a14	A	T	4: 115,496,100 (GRCm38)	L5*	probably null	Het
Cyp4a30b	A	T	4: 115,459,391 (GRCm38)	K405*	probably null	Het
Dchs1	T	C	7: 105,754,095 (GRCm38)	D3080G	probably benign	Het
Ddx1	T	C	12: 13,237,799 (GRCm38)	D168G	probably damaging	Het
Dhx57	T	G	17: 80,245,806 (GRCm38)	K1231Q	probably damaging	Het
Dnah14	A	G	1: 181,770,105 (GRCm38)	N3640D	probably benign	Het
Dock4	T	A	12: 40,755,834 (GRCm38)	L935Q	probably damaging	Het
Dus2	A	T	8: 106,046,066 (GRCm38)	R269S	probably benign	Het
E230025N22Rik	G	A	18: 36,689,108 (GRCm38)	R201C	possibly damaging	Het
Erbb3	G	A	10: 128,583,185 (GRCm38)	T269M	probably damaging	Het
Fam71f2	T	A	6: 29,290,424 (GRCm38)	L267*	probably null	Het
Fbxo43	T	C	15: 36,162,093 (GRCm38)	R323G	probably damaging	Het
Fktn	A	G	4: 53,735,061 (GRCm38)	H233R	probably benign	Het
Ftsj3	A	T	11: 106,252,251 (GRCm38)	D412E	probably damaging	Het
Fzd7	A	T	1: 59,484,544 (GRCm38)	M529L	probably benign	Het
Fzr1	T	A	10: 81,370,826 (GRCm38)		probably null	Het
Ganc	T	G	2: 120,430,605 (GRCm38)	V257G	possibly damaging	Het
Gm4131	T	C	14: 62,464,758 (GRCm38)	K254E	probably damaging	Het
Gm6583	C	T	5: 112,355,008 (GRCm38)	V277M	possibly damaging	Het
Gm7347	G	T	5: 26,057,249 (GRCm38)	Y91*	probably null	Het
Gm9857	G	A	3: 108,940,165 (GRCm38)		probably benign	Het
Grpel1	T	C	5: 36,465,248 (GRCm38)	S19P	probably benign	Het
Gtf3c4	T	C	2: 28,833,711 (GRCm38)	K670E	possibly damaging	Het
Hist1h2bf	G	A	13: 23,574,103 (GRCm38)		probably benign	Het
Hmcn1	G	T	1: 150,704,173 (GRCm38)	Q1938K	possibly damaging	Het
Hnrnpk	T	C	13: 58,399,157 (GRCm38)	D71G	probably damaging	Het
Hspa4l	G	A	3: 40,767,979 (GRCm38)	R311H	probably damaging	Het
Igkv3-5	T	A	6: 70,663,704 (GRCm38)	F56L	probably benign	Het
Igkv6-20	T	A	6: 70,335,914 (GRCm38)	T92S	possibly damaging	Het
Krt8	C	T	15: 102,000,594 (GRCm38)	V200I	possibly damaging	Het
Lamb2	A	T	9: 108,480,388 (GRCm38)	T66S	possibly damaging	Het
Lamp3	A	G	16: 19,701,028 (GRCm38)	L135S	probably benign	Het
Lrguk	A	G	6: 34,129,143 (GRCm38)	Y701C	probably damaging	Het
Mcc	G	T	18: 44,449,321 (GRCm38)	L588M	probably damaging	Het
Mcidas	T	A	13: 112,998,586 (GRCm38)	L234Q	probably damaging	Het
Mtmr14	T	A	6: 113,280,614 (GRCm38)	L208Q	probably damaging	Het
Myof	A	G	19: 37,905,299 (GRCm38)	F1139L	possibly damaging	Het
Nlrp14	C	A	7: 107,182,496 (GRCm38)	T300K	probably benign	Het
Olfr1474	A	G	19: 13,471,506 (GRCm38)	I179V	probably benign	Het
Olfr165	T	A	16: 19,407,944 (GRCm38)	H24L	probably benign	Het
Olfr716	A	T	7: 107,147,328 (GRCm38)	E4V	possibly damaging	Het
Olfr890	A	T	9: 38,143,801 (GRCm38)	Y217F	probably damaging	Het
Orc2	A	G	1: 58,472,388 (GRCm38)	I354T	probably damaging	Het
Pard3b	G	T	1: 62,076,409 (GRCm38)	S140I	probably damaging	Het
Pcgf5	A	G	19: 36,434,603 (GRCm38)	D49G	probably benign	Het
Pcsk6	T	C	7: 65,959,293 (GRCm38)	F388S	probably damaging	Het
Prokr2	A	C	2: 132,381,442 (GRCm38)	I60S	probably damaging	Het
Rab33b	A	G	3: 51,484,479 (GRCm38)	T50A	possibly damaging	Het
Rbms3	T	C	9: 116,719,389 (GRCm38)	D61G	probably damaging	Het
Rhcg	T	A	7: 79,600,514 (GRCm38)	K274*	probably null	Het
Rnf112	C	T	11: 61,451,022 (GRCm38)	V319M	possibly damaging	Het
Rnf44	A	T	13: 54,682,800 (GRCm38)	S265T	possibly damaging	Het
Sf3a3	A	G	4: 124,722,058 (GRCm38)	D168G	probably damaging	Het
Sik2	A	G	9: 50,895,342 (GRCm38)		probably null	Het
Slco1a5	T	A	6: 142,253,113 (GRCm38)	L275F	probably benign	Het
Smtnl1	T	C	2: 84,815,378 (GRCm38)	H383R	probably damaging	Het
Spns3	T	G	11: 72,539,078 (GRCm38)	T175P	probably damaging	Het
Togaram1	A	G	12: 64,995,538 (GRCm38)	T1174A	probably benign	Het
Tradd	C	T	8: 105,260,645 (GRCm38)	E10K	possibly damaging	Het
Ttc7b	A	T	12: 100,373,560 (GRCm38)	Y579N	probably damaging	Het
Uncx	G	A	5: 139,547,589 (GRCm38)	G470R	probably damaging	Het
Vav3	T	A	3: 109,501,461 (GRCm38)	M177K	probably damaging	Het
Wfs1	A	T	5: 36,967,750 (GRCm38)	I599N	probably damaging	Het
Zfp454	G	A	11: 50,873,622 (GRCm38)	H217Y	probably damaging	Het

Other mutations in Adgrf3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03080:Adgrf3	APN	5	30,196,829 (GRCm38)	missense	probably benign	0.02
IGL03171:Adgrf3	APN	5	30,196,294 (GRCm38)	missense	probably damaging	1.00
R0010:Adgrf3	UTSW	5	30,205,609 (GRCm38)	splice site	probably benign
R0042:Adgrf3	UTSW	5	30,197,428 (GRCm38)	missense	probably damaging	1.00
R0140:Adgrf3	UTSW	5	30,196,381 (GRCm38)	missense	probably benign	0.19
R0617:Adgrf3	UTSW	5	30,195,080 (GRCm38)	missense	probably benign	0.25
R0748:Adgrf3	UTSW	5	30,196,876 (GRCm38)	missense	probably damaging	1.00
R1291:Adgrf3	UTSW	5	30,199,534 (GRCm38)	missense	probably damaging	0.99
R1330:Adgrf3	UTSW	5	30,195,095 (GRCm38)	missense	probably benign	0.24
R1468:Adgrf3	UTSW	5	30,202,229 (GRCm38)	splice site	probably benign
R1695:Adgrf3	UTSW	5	30,203,555 (GRCm38)	missense	probably benign	0.05
R1716:Adgrf3	UTSW	5	30,197,551 (GRCm38)	missense	probably benign	0.03
R1844:Adgrf3	UTSW	5	30,199,213 (GRCm38)	missense	probably damaging	0.96
R1935:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R1936:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R2059:Adgrf3	UTSW	5	30,199,491 (GRCm38)	missense	possibly damaging	0.91
R2656:Adgrf3	UTSW	5	30,196,438 (GRCm38)	missense	possibly damaging	0.96
R2913:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2914:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2987:Adgrf3	UTSW	5	30,197,360 (GRCm38)	missense	probably damaging	1.00
R3797:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3798:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3799:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3934:Adgrf3	UTSW	5	30,200,434 (GRCm38)	unclassified	probably benign
R4043:Adgrf3	UTSW	5	30,204,362 (GRCm38)	missense	probably benign	0.00
R4080:Adgrf3	UTSW	5	30,197,369 (GRCm38)	nonsense	probably null
R4575:Adgrf3	UTSW	5	30,202,257 (GRCm38)	missense	probably benign	0.00
R4754:Adgrf3	UTSW	5	30,197,617 (GRCm38)	critical splice acceptor site	probably null
R4819:Adgrf3	UTSW	5	30,198,444 (GRCm38)	missense	possibly damaging	0.66
R4893:Adgrf3	UTSW	5	30,200,478 (GRCm38)	missense	probably benign	0.00
R4991:Adgrf3	UTSW	5	30,199,148 (GRCm38)	missense	probably benign	0.26
R5686:Adgrf3	UTSW	5	30,197,306 (GRCm38)	missense	probably damaging	1.00
R5965:Adgrf3	UTSW	5	30,205,639 (GRCm38)	missense	probably benign	0.00
R6103:Adgrf3	UTSW	5	30,196,267 (GRCm38)	missense	probably damaging	1.00
R6244:Adgrf3	UTSW	5	30,197,533 (GRCm38)	missense	probably benign	0.17
R6409:Adgrf3	UTSW	5	30,197,314 (GRCm38)	missense	probably damaging	0.96
R6575:Adgrf3	UTSW	5	30,196,524 (GRCm38)	missense	possibly damaging	0.72
R6745:Adgrf3	UTSW	5	30,203,603 (GRCm38)	missense	probably benign	0.31
R6790:Adgrf3	UTSW	5	30,196,387 (GRCm38)	missense	probably benign	0.00
R6813:Adgrf3	UTSW	5	30,197,521 (GRCm38)	missense	probably damaging	0.96
R7202:Adgrf3	UTSW	5	30,204,380 (GRCm38)	nonsense	probably null
R7250:Adgrf3	UTSW	5	30,195,682 (GRCm38)	missense	probably damaging	1.00
R7353:Adgrf3	UTSW	5	30,198,497 (GRCm38)	missense	probably damaging	0.98
R7634:Adgrf3	UTSW	5	30,202,247 (GRCm38)	missense	probably benign	0.01
R7658:Adgrf3	UTSW	5	30,197,206 (GRCm38)	missense	probably benign	0.41
R8037:Adgrf3	UTSW	5	30,199,512 (GRCm38)	missense	probably damaging	1.00
R8281:Adgrf3	UTSW	5	30,197,303 (GRCm38)	missense	possibly damaging	0.46
R8717:Adgrf3	UTSW	5	30,198,581 (GRCm38)	unclassified	probably benign
R8857:Adgrf3	UTSW	5	30,197,067 (GRCm38)	nonsense	probably null
R8926:Adgrf3	UTSW	5	30,200,448 (GRCm38)	missense	possibly damaging	0.46
R9391:Adgrf3	UTSW	5	30,195,073 (GRCm38)	missense	possibly damaging	0.94
R9446:Adgrf3	UTSW	5	30,196,959 (GRCm38)	missense	probably benign	0.01
R9522:Adgrf3	UTSW	5	30,199,484 (GRCm38)	missense	possibly damaging	0.90
Z1088:Adgrf3	UTSW	5	30,199,120 (GRCm38)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- TTCTGGCCTCTCAGCATCAG -3'
(R):5'- GATTTTCATGTCACCCACGG -3'

Sequencing Primer
(F):5'- GCATCAGGCTTTCTTTATTCTCTATG -3'
(R):5'- ACGGTCCTTTCTGTCAGCTG -3'

Posted On

2017-06-26