Incidental Mutation 'R5997:Ftsj3'
ID 480639
Institutional Source Beutler Lab
Gene Symbol Ftsj3
Ensembl Gene ENSMUSG00000020706
Gene Name FtsJ RNA 2'-O-methyltransferase 3
Synonyms D11Ertd400e, C79843, Epcs3
MMRRC Submission 044176-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.971) question?
Stock # R5997 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 106139968-106146905 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 106143077 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 412 (D412E)
Ref Sequence ENSEMBL: ENSMUSP00000021048 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000021046] [ENSMUST00000021048] [ENSMUST00000021049] [ENSMUST00000133131]
AlphaFold Q9DBE9
Predicted Effect probably benign
Transcript: ENSMUST00000021046
SMART Domains Protein: ENSMUSP00000021046
Gene: ENSMUSG00000020705

DomainStartEndE-ValueType
low complexity region 6 19 N/A INTRINSIC
low complexity region 35 52 N/A INTRINSIC
low complexity region 68 82 N/A INTRINSIC
low complexity region 108 114 N/A INTRINSIC
coiled coil region 116 143 N/A INTRINSIC
low complexity region 149 158 N/A INTRINSIC
DEXDc 272 474 7.61e-68 SMART
HELICc 512 593 1.58e-33 SMART
low complexity region 644 659 N/A INTRINSIC
low complexity region 722 737 N/A INTRINSIC
low complexity region 814 838 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000021048
AA Change: D412E

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000021048
Gene: ENSMUSG00000020706
AA Change: D412E

DomainStartEndE-ValueType
Pfam:FtsJ 24 200 2.8e-56 PFAM
low complexity region 203 218 N/A INTRINSIC
Pfam:DUF3381 231 398 1.3e-48 PFAM
low complexity region 456 475 N/A INTRINSIC
low complexity region 560 568 N/A INTRINSIC
Pfam:Spb1_C 597 831 1.8e-72 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000021049
SMART Domains Protein: ENSMUSP00000021049
Gene: ENSMUSG00000020708

DomainStartEndE-ValueType
low complexity region 57 69 N/A INTRINSIC
low complexity region 96 108 N/A INTRINSIC
AAA 182 321 6.96e-25 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126938
Predicted Effect noncoding transcript
Transcript: ENSMUST00000127803
Predicted Effect probably benign
Transcript: ENSMUST00000133131
SMART Domains Protein: ENSMUSP00000138057
Gene: ENSMUSG00000020708

DomainStartEndE-ValueType
low complexity region 57 69 N/A INTRINSIC
low complexity region 96 108 N/A INTRINSIC
AAA 182 321 6.96e-25 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000143729
Predicted Effect noncoding transcript
Transcript: ENSMUST00000154635
Predicted Effect noncoding transcript
Transcript: ENSMUST00000143884
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.4%
  • 10x: 97.1%
  • 20x: 90.5%
Validation Efficiency 100% (78/78)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Although the function of this gene is not known, the existence of this gene is supported by mRNA and EST data. A possible function of the encoded protein can be inferred from amino acid sequence similarity to the E.coli FtsJ protein and to a mouse protein possibly involved in embryogenesis. [provided by RefSeq, Jul 2008]
Allele List at MGI

All alleles(10) : Targeted(2) Gene trapped(8)

Other mutations in this stock
Total: 78 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb9 A T 5: 124,227,878 (GRCm39) V121E possibly damaging Het
Acsbg3 A T 17: 57,183,373 (GRCm39) D38V probably benign Het
Adamts20 T A 15: 94,277,628 (GRCm39) Y278F probably damaging Het
Adcy7 A T 8: 89,053,020 (GRCm39) D972V probably benign Het
Adgrf3 C A 5: 30,403,360 (GRCm39) probably null Het
Ahdc1 G T 4: 132,791,206 (GRCm39) G816C probably benign Het
Aifm3 A G 16: 17,319,994 (GRCm39) K283E probably benign Het
Akap6 T C 12: 52,984,016 (GRCm39) probably null Het
Ank1 T C 8: 23,589,678 (GRCm39) L593P probably damaging Het
Apol11b T A 15: 77,519,697 (GRCm39) T128S probably benign Het
C1qtnf7 C A 5: 43,773,427 (GRCm39) T235K probably damaging Het
Camk2a A T 18: 61,111,029 (GRCm39) I73F probably damaging Het
Ccdc121rt3 C T 5: 112,502,874 (GRCm39) V277M possibly damaging Het
Cd109 G T 9: 78,612,344 (GRCm39) V1244F possibly damaging Het
Cep164 A G 9: 45,680,761 (GRCm39) L1240S possibly damaging Het
Cnga1 T A 5: 72,761,918 (GRCm39) D532V probably damaging Het
Cyp4a14 A T 4: 115,353,297 (GRCm39) L5* probably null Het
Cyp4a30b A T 4: 115,316,588 (GRCm39) K405* probably null Het
Dchs1 T C 7: 105,403,302 (GRCm39) D3080G probably benign Het
Ddx1 T C 12: 13,287,800 (GRCm39) D168G probably damaging Het
Dhx57 T G 17: 80,553,235 (GRCm39) K1231Q probably damaging Het
Dnah14 A G 1: 181,597,670 (GRCm39) N3640D probably benign Het
Dock4 T A 12: 40,805,833 (GRCm39) L935Q probably damaging Het
Dus2 A T 8: 106,772,698 (GRCm39) R269S probably benign Het
E230025N22Rik G A 18: 36,822,161 (GRCm39) R201C possibly damaging Het
Erbb3 G A 10: 128,419,054 (GRCm39) T269M probably damaging Het
Fbxo43 T C 15: 36,162,239 (GRCm39) R323G probably damaging Het
Fktn A G 4: 53,735,061 (GRCm39) H233R probably benign Het
Fzd7 A T 1: 59,523,703 (GRCm39) M529L probably benign Het
Fzr1 T A 10: 81,206,660 (GRCm39) probably null Het
Ganc T G 2: 120,261,086 (GRCm39) V257G possibly damaging Het
Garin1a T A 6: 29,290,423 (GRCm39) L267* probably null Het
Gm4131 T C 14: 62,702,207 (GRCm39) K254E probably damaging Het
Gm7347 G T 5: 26,262,247 (GRCm39) Y91* probably null Het
Gm9857 G A 3: 108,847,481 (GRCm39) probably benign Het
Grpel1 T C 5: 36,622,592 (GRCm39) S19P probably benign Het
Gtf3c4 T C 2: 28,723,723 (GRCm39) K670E possibly damaging Het
H2bc7 G A 13: 23,758,277 (GRCm39) probably benign Het
Hmcn1 G T 1: 150,579,924 (GRCm39) Q1938K possibly damaging Het
Hnrnpk T C 13: 58,546,971 (GRCm39) D71G probably damaging Het
Hspa4l G A 3: 40,722,411 (GRCm39) R311H probably damaging Het
Igkv3-5 T A 6: 70,640,688 (GRCm39) F56L probably benign Het
Igkv6-20 T A 6: 70,312,898 (GRCm39) T92S possibly damaging Het
Krt8 C T 15: 101,909,029 (GRCm39) V200I possibly damaging Het
Lamb2 A T 9: 108,357,587 (GRCm39) T66S possibly damaging Het
Lamp3 A G 16: 19,519,778 (GRCm39) L135S probably benign Het
Lrguk A G 6: 34,106,078 (GRCm39) Y701C probably damaging Het
Mcc G T 18: 44,582,388 (GRCm39) L588M probably damaging Het
Mcidas T A 13: 113,135,120 (GRCm39) L234Q probably damaging Het
Mtmr14 T A 6: 113,257,575 (GRCm39) L208Q probably damaging Het
Myof A G 19: 37,893,747 (GRCm39) F1139L possibly damaging Het
Nlrp14 C A 7: 106,781,703 (GRCm39) T300K probably benign Het
Or2d36 A T 7: 106,746,535 (GRCm39) E4V possibly damaging Het
Or2m13 T A 16: 19,226,694 (GRCm39) H24L probably benign Het
Or5b118 A G 19: 13,448,870 (GRCm39) I179V probably benign Het
Or8b41 A T 9: 38,055,097 (GRCm39) Y217F probably damaging Het
Orc2 A G 1: 58,511,547 (GRCm39) I354T probably damaging Het
Pard3b G T 1: 62,115,568 (GRCm39) S140I probably damaging Het
Pcgf5 A G 19: 36,412,003 (GRCm39) D49G probably benign Het
Pcsk6 T C 7: 65,609,041 (GRCm39) F388S probably damaging Het
Prokr2 A C 2: 132,223,362 (GRCm39) I60S probably damaging Het
Rab33b A G 3: 51,391,900 (GRCm39) T50A possibly damaging Het
Rbms3 T C 9: 116,548,457 (GRCm39) D61G probably damaging Het
Rhcg T A 7: 79,250,262 (GRCm39) K274* probably null Het
Rnf112 C T 11: 61,341,848 (GRCm39) V319M possibly damaging Het
Rnf44 A T 13: 54,830,613 (GRCm39) S265T possibly damaging Het
Sf3a3 A G 4: 124,615,851 (GRCm39) D168G probably damaging Het
Sik2 A G 9: 50,806,642 (GRCm39) probably null Het
Slco1a5 T A 6: 142,198,839 (GRCm39) L275F probably benign Het
Smtnl1 T C 2: 84,645,722 (GRCm39) H383R probably damaging Het
Spns3 T G 11: 72,429,904 (GRCm39) T175P probably damaging Het
Togaram1 A G 12: 65,042,312 (GRCm39) T1174A probably benign Het
Tradd C T 8: 105,987,277 (GRCm39) E10K possibly damaging Het
Ttc7b A T 12: 100,339,819 (GRCm39) Y579N probably damaging Het
Uncx G A 5: 139,533,344 (GRCm39) G470R probably damaging Het
Vav3 T A 3: 109,408,777 (GRCm39) M177K probably damaging Het
Wfs1 A T 5: 37,125,094 (GRCm39) I599N probably damaging Het
Zfp454 G A 11: 50,764,449 (GRCm39) H217Y probably damaging Het
Other mutations in Ftsj3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00719:Ftsj3 APN 11 106,141,005 (GRCm39) missense probably damaging 0.99
IGL00964:Ftsj3 APN 11 106,143,941 (GRCm39) missense probably benign 0.00
IGL01025:Ftsj3 APN 11 106,141,185 (GRCm39) missense probably damaging 0.98
IGL01101:Ftsj3 APN 11 106,146,458 (GRCm39) missense probably benign 0.16
IGL01370:Ftsj3 APN 11 106,143,145 (GRCm39) missense possibly damaging 0.79
IGL02000:Ftsj3 APN 11 106,141,233 (GRCm39) missense probably benign 0.03
IGL02139:Ftsj3 APN 11 106,145,489 (GRCm39) missense possibly damaging 0.91
IGL02340:Ftsj3 APN 11 106,143,972 (GRCm39) nonsense probably null
IGL02964:Ftsj3 APN 11 106,143,163 (GRCm39) missense probably damaging 1.00
IGL03136:Ftsj3 APN 11 106,144,639 (GRCm39) missense probably damaging 1.00
D4043:Ftsj3 UTSW 11 106,145,634 (GRCm39) missense possibly damaging 0.91
LCD18:Ftsj3 UTSW 11 106,140,885 (GRCm39) splice site probably benign
NA:Ftsj3 UTSW 11 106,145,634 (GRCm39) missense possibly damaging 0.91
P0018:Ftsj3 UTSW 11 106,145,634 (GRCm39) missense possibly damaging 0.91
P0027:Ftsj3 UTSW 11 106,145,634 (GRCm39) missense possibly damaging 0.91
PIT4581001:Ftsj3 UTSW 11 106,140,302 (GRCm39) missense unknown
R1449:Ftsj3 UTSW 11 106,143,826 (GRCm39) missense probably benign 0.28
R2242:Ftsj3 UTSW 11 106,141,604 (GRCm39) missense probably benign 0.45
R4086:Ftsj3 UTSW 11 106,140,395 (GRCm39) missense probably damaging 1.00
R4356:Ftsj3 UTSW 11 106,144,502 (GRCm39) missense probably benign 0.01
R4358:Ftsj3 UTSW 11 106,144,502 (GRCm39) missense probably benign 0.01
R4943:Ftsj3 UTSW 11 106,140,344 (GRCm39) missense probably damaging 1.00
R5520:Ftsj3 UTSW 11 106,146,414 (GRCm39) missense probably benign 0.05
R6047:Ftsj3 UTSW 11 106,143,144 (GRCm39) missense probably damaging 0.96
R6180:Ftsj3 UTSW 11 106,144,166 (GRCm39) splice site probably null
R6771:Ftsj3 UTSW 11 106,140,366 (GRCm39) missense probably damaging 1.00
R7121:Ftsj3 UTSW 11 106,143,123 (GRCm39) missense probably damaging 1.00
R7303:Ftsj3 UTSW 11 106,145,506 (GRCm39) missense probably damaging 1.00
R7349:Ftsj3 UTSW 11 106,140,572 (GRCm39) missense probably damaging 1.00
R7559:Ftsj3 UTSW 11 106,143,813 (GRCm39) missense possibly damaging 0.55
R7782:Ftsj3 UTSW 11 106,143,377 (GRCm39) unclassified probably benign
R7783:Ftsj3 UTSW 11 106,143,377 (GRCm39) unclassified probably benign
R7899:Ftsj3 UTSW 11 106,143,115 (GRCm39) nonsense probably null
R8129:Ftsj3 UTSW 11 106,144,657 (GRCm39) missense probably benign 0.03
R8897:Ftsj3 UTSW 11 106,144,602 (GRCm39) missense probably damaging 1.00
R8933:Ftsj3 UTSW 11 106,141,660 (GRCm39) missense probably benign 0.01
R8985:Ftsj3 UTSW 11 106,141,595 (GRCm39) missense possibly damaging 0.82
Predicted Primers PCR Primer
(F):5'- TTCTGGAGTCTCGGGTACTC -3'
(R):5'- TCTGGCAGAGATGAAAGCCC -3'

Sequencing Primer
(F):5'- GGAGTCTCGGGTACTCCTTTTC -3'
(R):5'- CCAAGAAGTGGCAGAACTCAAG -3'
Posted On 2017-06-26