Mutagenetix > Incidental Mutation

Incidental Mutation 'R5992:Vmn2r3'

480870

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5992 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64258961-64287495 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64259647 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 688 (C688S)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably damaging
Transcript: ENSMUST00000170244
AA Change: C660S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: C660S

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000176328
AA Change: C688S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: C688S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	T	A	17: 45,508,623	L188*	probably null	Het
Acod1	C	T	14: 103,055,035	R332C	probably damaging	Het
Adamts3	T	A	5: 89,691,335	K852M	probably damaging	Het
Adm	A	T	7: 110,627,696		probably benign	Het
Aff4	A	G	11: 53,373,010	S286G	probably damaging	Het
Ank2	A	G	3: 126,959,651		probably null	Het
Aqr	A	T	2: 114,143,049	Y427*	probably null	Het
Arid1b	C	A	17: 4,994,956		probably benign	Het
Arpp21	T	A	9: 112,143,485	R259*	probably null	Het
Cep290	C	T	10: 100,543,321	A55V	possibly damaging	Het
Chsy3	GT	G	18: 59,176,166	163	probably null	Het
Clcn2	T	C	16: 20,713,654	E68G	possibly damaging	Het
Corin	T	A	5: 72,316,389	H699L	probably benign	Het
Cyld	T	C	8: 88,733,053	Y446H	probably damaging	Het
Dcst1	T	A	3: 89,352,576	E613V	probably damaging	Het
Dlk1	T	C	12: 109,455,581	C74R	probably damaging	Het
Dnah12	A	C	14: 26,697,341	K128T	probably benign	Het
Dspp	T	A	5: 104,178,451	S893R	unknown	Het
Dtl	A	T	1: 191,568,572		probably null	Het
F2rl1	T	A	13: 95,514,270	S35C	probably benign	Het
Fcgbp	A	T	7: 28,120,534	Y2562F	probably benign	Het
Fgf10	A	T	13: 118,715,508	D42V	probably benign	Het
Gm10271	A	T	10: 116,972,592	F6L	probably damaging	Het
Gm21190	T	A	5: 15,524,851	E256D	probably damaging	Het
Gm5157	A	G	7: 21,185,421	S66P	probably damaging	Het
Gm884	A	T	11: 103,613,792	M2450K	possibly damaging	Het
Hal	T	G	10: 93,490,916	L138R	probably damaging	Het
Hsd17b3	T	C	13: 64,059,470		probably null	Het
Lrsam1	T	C	2: 32,955,222	T94A	probably benign	Het
Macc1	T	C	12: 119,447,585	V696A	probably damaging	Het
Magi2	G	A	5: 19,227,291	M1I	probably null	Het
March7	C	A	2: 60,245,220	N674K	probably benign	Het
Mfap1b	A	G	2: 121,470,295	V34A	probably benign	Het
Mob3b	G	A	4: 35,084,069	S40L	probably benign	Het
Ndufs2	A	T	1: 171,236,418	V386E	probably damaging	Het
Nfic	C	T	10: 81,420,747	A19T	probably damaging	Het
Nfs1	G	A	2: 156,134,453	R174W	probably damaging	Het
Nin	G	T	12: 70,045,524	S670R	possibly damaging	Het
Nrxn1	T	C	17: 90,623,507	I754V	probably benign	Het
Nwd1	T	C	8: 72,653,573		probably null	Het
Olfr1231	T	A	2: 89,303,359	T78S	possibly damaging	Het
Olfr1238	T	A	2: 89,406,879	M67L	probably benign	Het
Olfr575	G	T	7: 102,955,009	N197K	probably benign	Het
Pcdhgb8	A	G	18: 37,763,449	E524G	probably damaging	Het
Phlpp1	A	T	1: 106,318,993	R638*	probably null	Het
Pkmyt1	G	C	17: 23,735,326	W360S	probably benign	Het
Plxnd1	C	A	6: 115,967,787		probably null	Het
Poldip3	A	T	15: 83,129,229	N322K	probably damaging	Het
Prmt3	A	T	7: 49,828,947	I419L	probably benign	Het
Prss36	A	T	7: 127,944,830	V123E	probably damaging	Het
Prss58	A	G	6: 40,897,769	I46T	probably damaging	Het
Rac1	T	C	5: 143,506,998		probably benign	Het
Rb1cc1	A	G	1: 6,233,996	Y36C	probably damaging	Het
Rif1	C	G	2: 52,095,844	L614V	probably damaging	Het
Rnd2	C	T	11: 101,468,999	L57F	probably damaging	Het
Rp1	A	T	1: 4,148,703	F951L	unknown	Het
Rps6ka5	G	T	12: 100,575,250	P417T	possibly damaging	Het
Ryr1	A	G	7: 29,067,637	W2967R	probably damaging	Het
Sacs	T	A	14: 61,205,543	S1679R	probably damaging	Het
Scn1a	A	C	2: 66,335,456	W153G	probably damaging	Het
Serpinb7	A	G	1: 107,445,996	Y114C	probably damaging	Het
Son	T	C	16: 91,658,904	M1513T	probably benign	Het
Spag8	C	T	4: 43,651,534	V447M	probably benign	Het
St3gal2	T	A	8: 110,969,553	Y257N	probably damaging	Het
Tars	A	T	15: 11,397,196	D40E	probably damaging	Het
Tlr3	T	C	8: 45,397,814	H158R	probably benign	Het
Tppp2	G	T	14: 51,918,935	V50L	probably benign	Het
Trrap	T	C	5: 144,810,184	S1503P	probably benign	Het
Ttll4	T	G	1: 74,685,391	S573R	probably damaging	Het
Vmn2r104	A	T	17: 20,029,485	N841K	probably damaging	Het
Vps16	T	C	2: 130,424,449		probably null	Het
Zfp608	G	A	18: 54,899,248	T540I	probably benign	Het
Zfp775	G	A	6: 48,619,816	R208Q	probably damaging	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64259883	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64274961	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64275055	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64271199	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64287395	missense	probably benign
IGL02719:Vmn2r3	APN	3	64275610	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64259818	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64278835	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64275346	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64275366	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64275633	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64275276	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64275117	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64275472	missense	probably benign	0.03
R1806:Vmn2r3	UTSW	3	64287389	missense	possibly damaging	0.73
R1852:Vmn2r3	UTSW	3	64259394	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64259116	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64275072	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64259062	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64275312	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64275717	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64287429	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64275271	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64275601	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64259761	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64259197	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64271353	missense	probably benign
R5033:Vmn2r3	UTSW	3	64259799	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64259319	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64278826	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64259557	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64259023	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R6028:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64278761	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64275096	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64275618	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64274906	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64259506	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64259247	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64287311	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64259943	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64261404	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64275532	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64275349	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64275518	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64278814	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64275450	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64271293	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64274926	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64271196	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64278949	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64274890	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64259475	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64274962	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64287471	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64259274	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64259252	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64261382	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64278982	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64271314	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64271303	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64271248	missense	possibly damaging	0.62
X0022:Vmn2r3	UTSW	3	64274968	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAACATGCTGGGTGGCTTC -3'
(R):5'- ATTCTTTCCATATTTGGGGCACTTG -3'

Sequencing Primer
(F):5'- ATGCTGGGTGGCTTCAACAC -3'
(R):5'- CACTTGTGGTCTTGGCAGTTAC -3'

Posted On

2017-06-26