Incidental Mutation 'R5994:Zfp101'
ID481034
Institutional Source Beutler Lab
Gene Symbol Zfp101
Ensembl Gene ENSMUSG00000055240
Gene Namezinc finger protein 101
Synonyms
MMRRC Submission 044173-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.054) question?
Stock #R5994 (G1)
Quality Score225.009
Status Not validated
Chromosome17
Chromosomal Location33360507-33394641 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 33380962 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Leucine at position 607 (M607L)
Ref Sequence ENSEMBL: ENSMUSP00000132508 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000167107] [ENSMUST00000174417] [ENSMUST00000174512]
Predicted Effect probably benign
Transcript: ENSMUST00000167107
AA Change: M607L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000132508
Gene: ENSMUSG00000055240
AA Change: M607L

DomainStartEndE-ValueType
KRAB 10 62 9.34e-15 SMART
ZnF_C2H2 219 241 2.57e-3 SMART
ZnF_C2H2 247 269 6.32e-3 SMART
ZnF_C2H2 275 297 3.16e-3 SMART
ZnF_C2H2 302 322 1.65e2 SMART
ZnF_C2H2 330 352 6.75e0 SMART
ZnF_C2H2 358 380 2.75e-3 SMART
ZnF_C2H2 386 408 4.47e-3 SMART
ZnF_C2H2 414 436 2.09e-3 SMART
ZnF_C2H2 442 464 4.79e-3 SMART
ZnF_C2H2 470 492 2.2e-2 SMART
ZnF_C2H2 498 520 4.47e-3 SMART
ZnF_C2H2 526 548 4.72e-2 SMART
ZnF_C2H2 554 576 1.28e-3 SMART
ZnF_C2H2 583 605 5.06e-2 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000173151
Predicted Effect probably benign
Transcript: ENSMUST00000174417
SMART Domains Protein: ENSMUSP00000134459
Gene: ENSMUSG00000055240

DomainStartEndE-ValueType
Blast:KRAB 1 38 5e-16 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000174512
SMART Domains Protein: ENSMUSP00000133873
Gene: ENSMUSG00000055240

DomainStartEndE-ValueType
KRAB 10 62 9.34e-15 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000183047
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.4%
  • 20x: 91.7%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A2m A T 6: 121,670,903 H1118L probably benign Het
Abca8b A G 11: 109,949,766 probably null Het
Abcb5 A T 12: 118,965,260 probably null Het
Adcy6 A T 15: 98,593,664 I1016N probably damaging Het
Afg3l2 A T 18: 67,429,070 C312S probably damaging Het
Ano8 C T 8: 71,484,834 V89M probably damaging Het
Arhgap21 C T 2: 20,881,376 G330D possibly damaging Het
Caskin1 T C 17: 24,496,961 L195P probably damaging Het
Cfap54 A G 10: 93,039,081 I514T probably damaging Het
Ctdsp2 G A 10: 126,995,820 probably benign Het
Cyp4x1 T C 4: 115,121,945 I152V probably benign Het
Dglucy G A 12: 100,842,700 R219Q probably benign Het
Disp3 G T 4: 148,254,284 A810E possibly damaging Het
Dtx4 T C 19: 12,501,153 Y22C probably damaging Het
Edaradd A T 13: 12,478,496 I105N probably damaging Het
Eepd1 C T 9: 25,603,453 P519S probably damaging Het
Fscn3 A T 6: 28,430,295 S155C probably benign Het
Gm10134 A T 2: 28,506,246 E51V probably damaging Het
Gm7247 C T 14: 51,364,348 S26F probably benign Het
Golga7 T C 8: 23,250,265 E83G probably benign Het
Gpr12 T C 5: 146,583,431 H227R probably damaging Het
Hoxa2 T G 6: 52,164,392 S85R possibly damaging Het
Hrnr T C 3: 93,332,300 S3282P unknown Het
Ift74 C A 4: 94,691,724 T543K possibly damaging Het
Klf10 C A 15: 38,296,041 R420L probably damaging Het
Krt77 T A 15: 101,862,855 I338F probably damaging Het
Limch1 A T 5: 66,974,622 S152C probably damaging Het
Mgat4e T A 1: 134,541,496 H270L probably benign Het
Myrf A T 19: 10,219,117 L504Q probably null Het
Nckipsd A G 9: 108,813,977 Q366R probably benign Het
Npy5r A T 8: 66,682,099 V14D probably benign Het
Nrap T A 19: 56,351,599 R830* probably null Het
Ogfrl1 A T 1: 23,378,989 Y103N probably damaging Het
Olfr978 A T 9: 39,994,223 R138* probably null Het
P2rx4 T A 5: 122,725,079 L232H probably damaging Het
Pabpc2 A T 18: 39,773,894 T71S probably benign Het
Paip2b C A 6: 83,808,885 S121I probably damaging Het
Pofut1 C T 2: 153,261,229 T261I possibly damaging Het
Ppp6c G T 2: 39,210,992 T46K possibly damaging Het
Prkd2 C A 7: 16,850,336 H371Q probably benign Het
Prrc2c A T 1: 162,674,156 probably null Het
Psd3 C T 8: 67,719,968 A894T probably damaging Het
Pygm A T 19: 6,398,043 probably null Het
Pzp A T 6: 128,491,597 M989K probably damaging Het
Ralgapa2 A T 2: 146,361,453 S1159T probably benign Het
Rapgefl1 T C 11: 98,850,160 F575L probably benign Het
Rassf6 A G 5: 90,617,768 L28S probably damaging Het
Rbp3 G T 14: 33,954,900 K268N probably damaging Het
Rela C T 19: 5,647,064 T433M possibly damaging Het
Rnf103 T A 6: 71,496,910 S102R probably damaging Het
Scarf2 A G 16: 17,806,379 N516S probably damaging Het
Sdcbp2 T C 2: 151,587,483 I241T probably damaging Het
Sept7 T C 9: 25,288,198 I131T possibly damaging Het
Sh3pxd2b T C 11: 32,407,570 F191L probably damaging Het
Siglec15 C A 18: 78,047,375 C236F probably damaging Het
Slc11a2 T C 15: 100,397,681 T520A probably benign Het
Slc26a11 C A 11: 119,379,912 F553L probably benign Het
Smchd1 G A 17: 71,365,409 P1596S possibly damaging Het
Taar7b A G 10: 24,000,348 H137R probably damaging Het
Thap12 T A 7: 98,716,030 C468* probably null Het
Timp4 C T 6: 115,247,354 G118D probably damaging Het
Tnnt3 A G 7: 142,511,266 K48E probably damaging Het
Trmt10a T A 3: 138,156,714 I255N probably damaging Het
Ttll10 T C 4: 156,048,732 probably null Het
Ube4b A G 4: 149,372,932 Y283H probably damaging Het
Ucp1 G T 8: 83,293,938 V126L possibly damaging Het
Unc13b T A 4: 43,172,596 probably benign Het
Vps13b T C 15: 35,875,772 S2768P probably damaging Het
Zcchc6 T G 13: 59,789,209 Y806S probably damaging Het
Zfp292 C T 4: 34,805,464 V2527M possibly damaging Het
Zfp503 T A 14: 21,985,562 T429S possibly damaging Het
Other mutations in Zfp101
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0189:Zfp101 UTSW 17 33382239 missense possibly damaging 0.53
R0254:Zfp101 UTSW 17 33380978 missense possibly damaging 0.71
R0448:Zfp101 UTSW 17 33382321 missense possibly damaging 0.73
R0696:Zfp101 UTSW 17 33381427 missense possibly damaging 0.86
R0834:Zfp101 UTSW 17 33382444 missense probably benign 0.33
R2000:Zfp101 UTSW 17 33381517 missense possibly damaging 0.92
R2366:Zfp101 UTSW 17 33380998 missense probably benign 0.06
R2471:Zfp101 UTSW 17 33380976 missense possibly damaging 0.85
R3713:Zfp101 UTSW 17 33381906 missense probably benign 0.05
R3857:Zfp101 UTSW 17 33382431 nonsense probably null
R4537:Zfp101 UTSW 17 33382492 missense possibly damaging 0.87
R4712:Zfp101 UTSW 17 33394483 splice site probably null
R5049:Zfp101 UTSW 17 33381898 missense possibly damaging 0.71
R5241:Zfp101 UTSW 17 33382236 missense probably benign
R5499:Zfp101 UTSW 17 33382344 missense probably benign 0.20
R5587:Zfp101 UTSW 17 33381321 missense possibly damaging 0.71
R5694:Zfp101 UTSW 17 33380945 missense probably benign
R6193:Zfp101 UTSW 17 33381746 nonsense probably null
R7331:Zfp101 UTSW 17 33382585 missense possibly damaging 0.53
R7393:Zfp101 UTSW 17 33386700 missense possibly damaging 0.71
R7434:Zfp101 UTSW 17 33381590 missense possibly damaging 0.86
R7922:Zfp101 UTSW 17 33381537 missense possibly damaging 0.93
R8087:Zfp101 UTSW 17 33381003 missense probably benign
R8684:Zfp101 UTSW 17 33382003 missense possibly damaging 0.86
Predicted Primers PCR Primer
(F):5'- GCTGGTCATGTCTGTTTCCAC -3'
(R):5'- GCCGTATACATGTAAGCATTGC -3'

Sequencing Primer
(F):5'- ATGACTAGCTAAGCTATCTCGCGTG -3'
(R):5'- TTCCAGTACCCGTAACAGTCATG -3'
Posted On2017-06-26