Incidental Mutation 'R5996:Ccr8'
ID 481135
Institutional Source Beutler Lab
Gene Symbol Ccr8
Ensembl Gene ENSMUSG00000042262
Gene Name chemokine (C-C motif) receptor 8
Synonyms Cmkbr8
MMRRC Submission 044175-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5996 (G1)
Quality Score 225.009
Status Validated
Chromosome 9
Chromosomal Location 120092114-120094906 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 120094463 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Cysteine to Serine at position 215 (C215S)
Ref Sequence ENSEMBL: ENSMUSP00000038473 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000048777]
AlphaFold P56484
Predicted Effect probably damaging
Transcript: ENSMUST00000048777
AA Change: C215S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000038473
Gene: ENSMUSG00000042262
AA Change: C215S

Pfam:7TM_GPCR_Srsx 44 313 2.4e-8 PFAM
Pfam:7tm_1 50 298 3.3e-44 PFAM
low complexity region 338 347 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000217495
Meta Mutation Damage Score 0.7887 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.6%
  • 20x: 92.4%
Validation Efficiency 97% (69/71)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for either of two independently generated knock-out alleles show normal lung eosinophilia and Th2 cytokine responses in OVA-elicited asthma models. Mice homozygous for a third knock-out allele show a delay in onset of clinical signs of experimental autoimmune encephalomyelitis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700093K21Rik A G 11: 23,518,928 (GRCm38) M1T probably null Het
4932438A13Rik T C 3: 36,931,116 (GRCm38) F865S probably benign Het
A2m T A 6: 121,659,394 (GRCm38) W741R probably damaging Het
Abcb8 C T 5: 24,400,139 (GRCm38) R108C possibly damaging Het
Acan A G 7: 79,111,320 (GRCm38) T1927A probably damaging Het
Agbl4 A G 4: 110,955,672 (GRCm38) probably null Het
Cd209b T C 8: 3,918,688 (GRCm38) T314A probably benign Het
Cd244 T G 1: 171,581,640 (GRCm38) probably null Het
Cdh23 T C 10: 60,413,577 (GRCm38) E849G possibly damaging Het
Cep57 A G 9: 13,809,879 (GRCm38) V268A probably damaging Het
Clip4 T C 17: 71,856,310 (GRCm38) S626P probably damaging Het
Col19a1 C T 1: 24,328,071 (GRCm38) G477R probably damaging Het
Col4a4 T C 1: 82,455,728 (GRCm38) D1498G unknown Het
Coro6 T C 11: 77,466,496 (GRCm38) V177A probably benign Het
Crip3 T C 17: 46,431,284 (GRCm38) S202P possibly damaging Het
Cxcr2 T C 1: 74,158,460 (GRCm38) C38R probably damaging Het
Cyp4f13 C T 17: 32,929,473 (GRCm38) G327R possibly damaging Het
Dab2 C T 15: 6,435,311 (GRCm38) Q415* probably null Het
Dennd5b A G 6: 149,068,095 (GRCm38) F287L probably benign Het
Dlg4 G A 11: 70,017,231 (GRCm38) R6K probably benign Het
Edc4 A G 8: 105,887,401 (GRCm38) K429E probably damaging Het
Fam107b T A 2: 3,779,630 (GRCm38) probably null Het
Flt4 A T 11: 49,651,070 (GRCm38) D1360V probably damaging Het
Gm1043 A T 5: 37,159,824 (GRCm38) probably benign Het
Gm35060 G T 18: 32,462,492 (GRCm38) probably benign Het
Gm4894 A T 9: 49,278,670 (GRCm38) I82L unknown Het
Gm7247 C T 14: 51,364,348 (GRCm38) S26F probably benign Het
Gpr176 A C 2: 118,283,904 (GRCm38) probably null Het
Gtse1 T C 15: 85,864,180 (GRCm38) L262P probably benign Het
Il4ra T C 7: 125,567,221 (GRCm38) W46R probably damaging Het
Itga11 A G 9: 62,755,673 (GRCm38) Y557C probably benign Het
Kcnab2 A C 4: 152,434,830 (GRCm38) probably null Het
Kcnh7 G A 2: 63,184,097 (GRCm38) probably benign Het
Ly9 T A 1: 171,601,828 (GRCm38) Q230L probably damaging Het
Map2 C A 1: 66,414,884 (GRCm38) H978N possibly damaging Het
Map2k6 A G 11: 110,497,906 (GRCm38) I225V possibly damaging Het
Map3k13 A G 16: 21,905,245 (GRCm38) N326D possibly damaging Het
Mdga2 A G 12: 66,797,763 (GRCm38) F85L probably benign Het
Mmp13 C T 9: 7,274,269 (GRCm38) P192L probably damaging Het
Mrpl37 T C 4: 107,066,507 (GRCm38) T97A probably benign Het
Mycbpap C T 11: 94,513,594 (GRCm38) G121D probably benign Het
Nabp1 A G 1: 51,471,385 (GRCm38) S153P probably benign Het
Nsun3 A T 16: 62,769,686 (GRCm38) F222I probably benign Het
Olfr735 A G 14: 50,345,512 (GRCm38) V310A possibly damaging Het
Pcdhga4 A T 18: 37,685,938 (GRCm38) D180V probably benign Het
Pex6 C A 17: 46,714,458 (GRCm38) probably null Het
Phrf1 C A 7: 141,259,102 (GRCm38) probably benign Het
Ppp1r36 A G 12: 76,439,162 (GRCm38) T365A possibly damaging Het
Ranbp10 A G 8: 105,773,040 (GRCm38) M519T probably benign Het
Rfx4 G A 10: 84,840,017 (GRCm38) W32* probably null Het
Ryr1 T C 7: 29,024,241 (GRCm38) N4131S probably benign Het
Slc27a6 A G 18: 58,612,234 (GRCm38) E580G possibly damaging Het
Slc7a14 T C 3: 31,209,236 (GRCm38) D719G probably benign Het
Slfn9 A G 11: 82,987,484 (GRCm38) I273T possibly damaging Het
Spice1 A G 16: 44,384,674 (GRCm38) E781G probably benign Het
Sspo C T 6: 48,494,176 (GRCm38) P4686S possibly damaging Het
Stab1 G T 14: 31,139,551 (GRCm38) R2500S probably benign Het
Stox2 A T 8: 47,203,147 (GRCm38) M98K possibly damaging Het
Tacc2 T C 7: 130,623,483 (GRCm38) S633P probably damaging Het
Tm9sf4 T C 2: 153,195,571 (GRCm38) probably null Het
Togaram2 T A 17: 71,704,783 (GRCm38) F486I probably damaging Het
Trappc12 A G 12: 28,747,114 (GRCm38) S140P possibly damaging Het
Ttc29 A T 8: 78,276,896 (GRCm38) N247I probably damaging Het
Uso1 A T 5: 92,192,730 (GRCm38) E668D probably benign Het
Vmn2r69 A T 7: 85,411,909 (GRCm38) probably null Het
Xirp2 T A 2: 67,511,650 (GRCm38) L1412I possibly damaging Het
Xrcc5 T A 1: 72,310,458 (GRCm38) D15E probably damaging Het
Zfp661 T C 2: 127,577,048 (GRCm38) K391E probably damaging Het
Zfp729b A G 13: 67,593,858 (GRCm38) I106T probably benign Het
Zfyve9 A C 4: 108,719,360 (GRCm38) S175A probably benign Het
Other mutations in Ccr8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01511:Ccr8 APN 9 120,094,625 (GRCm38) missense probably damaging 1.00
IGL02558:Ccr8 APN 9 120,094,658 (GRCm38) missense probably benign 0.01
IGL02966:Ccr8 APN 9 120,094,140 (GRCm38) missense probably damaging 0.96
IGL03135:Ccr8 APN 9 120,094,623 (GRCm38) missense possibly damaging 0.89
R0402:Ccr8 UTSW 9 120,094,910 (GRCm38) splice site probably null
R0739:Ccr8 UTSW 9 120,094,349 (GRCm38) missense probably damaging 1.00
R1069:Ccr8 UTSW 9 120,094,217 (GRCm38) missense probably benign 0.00
R4766:Ccr8 UTSW 9 120,094,464 (GRCm38) missense probably damaging 1.00
R4934:Ccr8 UTSW 9 120,094,749 (GRCm38) missense probably benign
R5116:Ccr8 UTSW 9 120,094,029 (GRCm38) missense probably benign 0.39
R5942:Ccr8 UTSW 9 120,094,706 (GRCm38) missense probably damaging 0.99
R5957:Ccr8 UTSW 9 120,093,827 (GRCm38) missense probably damaging 0.99
R7223:Ccr8 UTSW 9 120,094,617 (GRCm38) missense probably damaging 0.99
R8037:Ccr8 UTSW 9 120,094,370 (GRCm38) missense probably benign
R8332:Ccr8 UTSW 9 120,094,374 (GRCm38) missense probably damaging 1.00
R8962:Ccr8 UTSW 9 120,094,547 (GRCm38) missense possibly damaging 0.56
R9344:Ccr8 UTSW 9 120,094,067 (GRCm38) missense probably damaging 1.00
Z1177:Ccr8 UTSW 9 120,094,499 (GRCm38) missense probably benign 0.27
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2017-06-26