Mutagenetix > Incidental Mutation

Incidental Mutation 'R5979:Nrxn1'

481349

Institutional Source

Beutler Lab

Gene Symbol

Nrxn1

Ensembl Gene

ENSMUSG00000024109

Gene Name

neurexin I

Synonyms

neurexin I beta, alpha-latrotoxin receptor (calcium-dependent), A230068P09Rik, neurexin I alpha, neurexin I alpha, neurexin I beta, 1700062G21Rik, 9330127H16Rik, neurexin I alpha, neurexin I beta

MMRRC Submission

044161-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5979 (G1)

Quality Score

112.008

Status

Validated

Chromosome

Chromosomal Location

90033631-91093071 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 91088203 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 175 (R175H)

Ref Sequence

ENSEMBL: ENSMUSP00000133491 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054059] [ENSMUST00000072671] [ENSMUST00000095183] [ENSMUST00000160800] [ENSMUST00000160844] [ENSMUST00000161402] [ENSMUST00000174331]

AlphaFold

Q9CS84

Predicted Effect

possibly damaging
Transcript: ENSMUST00000054059
AA Change: R175H

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000057294
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
low complexity region	9	23	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	304	438	2.3e-36	SMART
LamG	492	644	2.74e-43	SMART
EGF	671	705	1.58e-3	SMART
LamG	730	869	7.27e-25	SMART
LamG	917	1053	8.46e-35	SMART
EGF	1078	1112	1.87e1	SMART
LamG	1140	1297	7.74e-20	SMART
low complexity region	1324	1355	N/A	INTRINSIC
low complexity region	1426	1441	N/A	INTRINSIC
4.1m	1444	1462	1.19e-6	SMART
low complexity region	1481	1493	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000072671
AA Change: R175H

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000072458
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
low complexity region	9	23	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	304	438	2.3e-36	SMART
LamG	492	644	2.74e-43	SMART
EGF	671	705	1.58e-3	SMART
LamG	730	869	7.27e-25	SMART
LamG	917	1053	8.46e-35	SMART
EGF	1078	1112	1.87e1	SMART
LamG	1140	1297	7.74e-20	SMART
low complexity region	1324	1355	N/A	INTRINSIC
low complexity region	1423	1438	N/A	INTRINSIC
4.1m	1441	1459	1.19e-6	SMART
low complexity region	1478	1490	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000095183

SMART Domains

Protein: ENSMUSP00000092806
Gene: ENSMUSG00000071033

Domain	Start	End	E-Value	Type
low complexity region	1	40	N/A	INTRINSIC
low complexity region	47	61	N/A	INTRINSIC
low complexity region	75	93	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159419

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160467

Predicted Effect

probably benign
Transcript: ENSMUST00000160800
AA Change: R175H

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000124561
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
low complexity region	9	23	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	300	434	2.3e-36	SMART
LamG	488	640	2.74e-43	SMART
EGF	667	701	1.58e-3	SMART
LamG	726	865	7.27e-25	SMART
LamG	913	1049	8.46e-35	SMART
EGF	1074	1108	1.87e1	SMART
LamG	1136	1293	7.74e-20	SMART
low complexity region	1320	1351	N/A	INTRINSIC
low complexity region	1422	1437	N/A	INTRINSIC
4.1m	1440	1458	1.19e-6	SMART
low complexity region	1477	1489	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160844
AA Change: R175H

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125407
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
low complexity region	9	23	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	304	446	1.24e-32	SMART
LamG	500	652	2.74e-43	SMART
EGF	679	713	1.58e-3	SMART
LamG	738	877	7.27e-25	SMART
LamG	925	1061	8.46e-35	SMART
EGF	1086	1120	1.87e1	SMART
LamG	1148	1305	7.74e-20	SMART
low complexity region	1332	1363	N/A	INTRINSIC
low complexity region	1434	1449	N/A	INTRINSIC
4.1m	1452	1470	1.19e-6	SMART
low complexity region	1489	1501	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161102

Predicted Effect

possibly damaging
Transcript: ENSMUST00000161402
AA Change: R175H

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000124116
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
low complexity region	9	23	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	304	453	3.46e-31	SMART
LamG	507	659	2.74e-43	SMART
EGF	686	720	1.58e-3	SMART
LamG	745	884	7.27e-25	SMART
LamG	932	1068	8.46e-35	SMART
EGF	1093	1127	1.87e1	SMART
LamG	1155	1312	7.74e-20	SMART
low complexity region	1339	1370	N/A	INTRINSIC
low complexity region	1441	1456	N/A	INTRINSIC
4.1m	1459	1477	1.19e-6	SMART
low complexity region	1496	1508	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161637

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162002

Predicted Effect

possibly damaging
Transcript: ENSMUST00000174331
AA Change: R175H

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000133491
Gene: ENSMUSG00000024109
AA Change: R175H

Domain	Start	End	E-Value	Type
signal peptide	1	30	N/A	INTRINSIC
LamG	50	192	2.29e-31	SMART
EGF	216	256	4.26e0	SMART
LamG	304	446	1.24e-32	SMART
LamG	500	652	2.74e-43	SMART
EGF	679	713	1.58e-3	SMART
LamG	738	877	7.27e-25	SMART
LamG	925	1061	8.46e-35	SMART
EGF	1086	1120	1.87e1	SMART
LamG	1148	1275	3.29e-23	SMART
low complexity region	1302	1333	N/A	INTRINSIC
low complexity region	1404	1419	N/A	INTRINSIC
4.1m	1422	1440	1.19e-6	SMART
low complexity region	1459	1471	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000198489

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.4%
20x: 95.4%

Validation Efficiency

100% (87/87)

MGI Phenotype

FUNCTION: This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are synaptic transmembrane receptors that bind endogenous ligands that include neuroligins, dystroglycan, and neurexophilins. Neurexin complexes are required for efficient neurotransmission and are involved in synaptogenesis. In vertebrates, alternate promoter usage results in multiple isoform classes, of which the alpha and beta classes are the best characterized. In humans, allelic variants in this gene are associated with Pitt-Hopkins-like syndrome-2, while deletions have been associated with autism and schizophrenia. Mouse knockouts display decreased spontaneous and evoked vesicle release resulting in impaired synaptic transmission. In addition, knockout mice show altered social approach, reduced social investigation, reduced locomotor activity, and in males, increased aggression. Alternative splicing and promoter usage result in multiple transcript variants. [provided by RefSeq, Nov 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced Ca(2+)-dependent binding of alpha-latrotoxin to brain membranes. Isolated synaptosomes display only a small reduction in alpha-latrotoxin -triggered glutamate release in the absence of Ca(2+) but show a major decrease in the presence of Ca(2+). [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acss2	A	G	2: 155,522,109	I103V	possibly damaging	Het
Adam3	T	C	8: 24,677,367	N36S	probably benign	Het
Adamts3	A	G	5: 89,861,669	V45A	probably damaging	Het
Afg3l2	G	T	18: 67,421,259	L458M	probably damaging	Het
Agtpbp1	A	T	13: 59,534,046	L69*	probably null	Het
Alkbh5	T	A	11: 60,538,691	I90N	probably damaging	Het
Alx1	T	C	10: 103,022,259	Y193C	probably damaging	Het
Ankrd11	T	G	8: 122,892,400	D1571A	probably damaging	Het
Brd4	T	C	17: 32,198,726	D124G	probably benign	Het
C2cd2	G	A	16: 97,875,218	T443I	probably benign	Het
Casp8	A	T	1: 58,828,912	M171L	probably benign	Het
Cd200r4	G	A	16: 44,832,932	V22I	probably benign	Het
Cdcp2	A	G	4: 107,105,281	Y217C	probably damaging	Het
Cfh	C	A	1: 140,118,671	V556F	possibly damaging	Het
Chka	T	G	19: 3,884,513	I182M	probably damaging	Het
Cope	T	C	8: 70,302,543		probably null	Het
Coq10b	T	C	1: 55,052,918	V15A	probably benign	Het
Cpne9	T	A	6: 113,293,749	S309T	probably benign	Het
Daam2	T	A	17: 49,459,204	H992L	possibly damaging	Het
Dctn3	T	C	4: 41,715,393		probably null	Het
Dhx35	G	T	2: 158,842,869	R536L	probably benign	Het
Dnah8	G	T	17: 30,815,664	E4186*	probably null	Het
Dnah9	A	G	11: 65,834,481	L4282P	probably damaging	Het
Dpp10	A	G	1: 123,384,283		probably null	Het
Dst	T	A	1: 34,160,372		probably benign	Het
Ehbp1	C	A	11: 22,151,887	V214L	probably benign	Het
Fam131b	T	C	6: 42,321,971	D25G	probably damaging	Het
Fbxl13	T	A	5: 21,582,091	I283F	probably damaging	Het
Gabrr3	T	G	16: 59,434,568	N205K	possibly damaging	Het
Got1l1	C	T	8: 27,197,923		probably null	Het
Gprin1	G	A	13: 54,739,978	A161V	probably benign	Het
Hepacam2	A	T	6: 3,476,149	F183I	probably damaging	Het
Hmx2	A	G	7: 131,554,550	T82A	probably benign	Het
Igsf10	C	T	3: 59,336,473	E147K	probably damaging	Het
Kndc1	G	A	7: 139,939,827	A1700T	probably benign	Het
Knl1	T	C	2: 119,069,360	V514A	possibly damaging	Het
Lama2	T	C	10: 27,235,732	D764G	probably damaging	Het
Lgi3	G	A	14: 70,536,460	R358H	probably damaging	Het
Limd1	G	T	9: 123,479,414	Q59H	possibly damaging	Het
Lrrk2	A	G	15: 91,772,945	Y1814C	possibly damaging	Het
Lysmd3	G	A	13: 81,665,274		probably null	Het
Mroh7	T	C	4: 106,720,926	N185S	probably benign	Het
Muc2	A	G	7: 141,697,250		probably null	Het
Muc2	G	A	7: 141,751,406	G149D	probably damaging	Het
Nlrp3	T	C	11: 59,548,971	F458S	probably benign	Het
Nop58	A	T	1: 59,702,831	D173V	probably damaging	Het
Nxpe4	A	G	9: 48,396,562	N322S	probably benign	Het
Ocstamp	A	G	2: 165,397,547	S240P	probably damaging	Het
Olfr137	T	C	17: 38,305,192	K90E	probably benign	Het
Olfr311	A	G	11: 58,841,840	H242R	probably damaging	Het
Olfr713	G	A	7: 107,036,336	M60I	probably damaging	Het
Olfr883	ATTGCTGTTT	ATTGCTGTTTGCTGTTT	9: 38,026,540		probably null	Het
Ovch2	G	A	7: 107,794,388	T177I	possibly damaging	Het
Parn	A	C	16: 13,606,171	L454R	probably damaging	Het
Pcdhb12	T	A	18: 37,437,991	L730Q	possibly damaging	Het
Phf3	G	T	1: 30,805,746	F1377L	probably damaging	Het
Pign	G	T	1: 105,589,274	S542R	probably benign	Het
Prex2	G	T	1: 11,132,372	V502F	probably damaging	Het
Psmd1	A	T	1: 86,090,053	I529F	possibly damaging	Het
Ptafr	A	G	4: 132,579,305	E2G	probably benign	Het
R3hdm1	A	G	1: 128,211,223	N380S	probably benign	Het
Rbm12	A	C	2: 156,097,759		probably benign	Het
Rgl1	A	G	1: 152,557,493	Y174H	probably damaging	Het
Rps6kc1	A	G	1: 190,800,435	S457P	probably damaging	Het
Sall4	A	T	2: 168,750,343	S964T	probably benign	Het
Sart1	T	A	19: 5,381,223	I681F	probably damaging	Het
Serinc5	T	C	13: 92,661,136	L49P	probably benign	Het
Serpinb9e	T	C	13: 33,255,053	V154A	probably benign	Het
Skiv2l	A	T	17: 34,841,463	N851K	probably benign	Het
Smox	G	A	2: 131,516,414	V136I	probably damaging	Het
Sspo	C	T	6: 48,463,693	T1747I	probably benign	Het
Swt1	A	T	1: 151,407,588	D339E	possibly damaging	Het
Synpo2	A	G	3: 123,117,411	L195P	probably damaging	Het
Syt7	G	T	19: 10,443,479	G414W	probably damaging	Het
Tmem186	G	A	16: 8,636,160	T79I	probably damaging	Het
Tmem39a	A	T	16: 38,575,744	N113I	probably damaging	Het
Trim14	C	T	4: 46,507,239	V326M	probably damaging	Het
Trim58	A	G	11: 58,646,083	E234G	probably damaging	Het
Ttr	T	C	18: 20,670,002	L75P	probably damaging	Het
Ubr1	C	T	2: 120,946,382	V293I	probably benign	Het
Vmn1r91	T	A	7: 20,102,065	V303E	probably benign	Het
Vmn2r30	A	C	7: 7,312,335	I833S	probably damaging	Het
Zfp131	G	T	13: 119,776,446	N125K	probably benign	Het
Zfp169	A	T	13: 48,491,040		probably benign	Het
Zfp213	C	A	17: 23,557,911	E386*	probably null	Het

Other mutations in Nrxn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Nrxn1	APN	17	90059474	critical splice donor site	probably null
IGL01644:Nrxn1	APN	17	90620873	missense	possibly damaging	0.94
IGL01820:Nrxn1	APN	17	90643103	missense	probably damaging	0.98
IGL01902:Nrxn1	APN	17	91088491	splice site	probably null
IGL02079:Nrxn1	APN	17	90643083	missense	probably damaging	0.99
IGL02089:Nrxn1	APN	17	91088401	missense	probably benign	0.01
IGL02133:Nrxn1	APN	17	90643243	missense	probably damaging	1.00
IGL02179:Nrxn1	APN	17	90630083	missense	probably damaging	0.99
IGL02199:Nrxn1	APN	17	90037258	missense	probably damaging	1.00
IGL02262:Nrxn1	APN	17	90704208	missense	probably damaging	1.00
IGL02941:Nrxn1	APN	17	90208383	missense	probably damaging	1.00
PIT4449001:Nrxn1	UTSW	17	90597579	missense	probably damaging	1.00
PIT4791001:Nrxn1	UTSW	17	90455503	intron	probably benign
R0123:Nrxn1	UTSW	17	90995487	splice site	probably null
R0212:Nrxn1	UTSW	17	90362758	unclassified	probably benign
R0277:Nrxn1	UTSW	17	90700742	critical splice donor site	probably null
R0323:Nrxn1	UTSW	17	90700742	critical splice donor site	probably null
R0384:Nrxn1	UTSW	17	90208347	missense	probably damaging	1.00
R0395:Nrxn1	UTSW	17	91088314	missense	possibly damaging	0.90
R0606:Nrxn1	UTSW	17	90565373	missense	probably damaging	1.00
R0616:Nrxn1	UTSW	17	90362857	missense	probably damaging	1.00
R0624:Nrxn1	UTSW	17	91088689	missense	unknown
R0633:Nrxn1	UTSW	17	90704181	missense	probably damaging	1.00
R0927:Nrxn1	UTSW	17	90037330	missense	probably damaging	1.00
R1035:Nrxn1	UTSW	17	90163874	missense	probably damaging	0.96
R1221:Nrxn1	UTSW	17	90643294	missense	probably damaging	0.97
R1403:Nrxn1	UTSW	17	90643053	missense	probably benign	0.11
R1403:Nrxn1	UTSW	17	90643053	missense	probably benign	0.11
R1691:Nrxn1	UTSW	17	90162289	missense	probably damaging	0.98
R1703:Nrxn1	UTSW	17	90208417	missense	probably damaging	1.00
R1709:Nrxn1	UTSW	17	90037187	missense	probably damaging	1.00
R1721:Nrxn1	UTSW	17	90162404	missense	probably damaging	1.00
R1792:Nrxn1	UTSW	17	90588824	missense	probably damaging	0.96
R1980:Nrxn1	UTSW	17	91088318	missense	probably benign	0.01
R2116:Nrxn1	UTSW	17	90704277	missense	probably damaging	1.00
R2117:Nrxn1	UTSW	17	90704277	missense	probably damaging	1.00
R2162:Nrxn1	UTSW	17	90162431	missense	probably damaging	1.00
R3119:Nrxn1	UTSW	17	90597519	nonsense	probably null
R3409:Nrxn1	UTSW	17	90208367	missense	probably damaging	1.00
R3683:Nrxn1	UTSW	17	90623452	missense	probably damaging	1.00
R3885:Nrxn1	UTSW	17	90623471	missense	probably damaging	1.00
R3939:Nrxn1	UTSW	17	90208421	missense	probably damaging	1.00
R4475:Nrxn1	UTSW	17	90701982	missense	probably damaging	0.98
R4640:Nrxn1	UTSW	17	90560768	missense	probably damaging	1.00
R4678:Nrxn1	UTSW	17	90623422	missense	probably damaging	1.00
R4690:Nrxn1	UTSW	17	90037081	missense	probably damaging	1.00
R4790:Nrxn1	UTSW	17	90455049	missense	possibly damaging	0.86
R4877:Nrxn1	UTSW	17	91088177	missense	probably benign	0.33
R4989:Nrxn1	UTSW	17	90620846	intron	probably benign
R5204:Nrxn1	UTSW	17	90162364	missense	probably damaging	1.00
R5205:Nrxn1	UTSW	17	90163874	missense	probably damaging	0.96
R5239:Nrxn1	UTSW	17	90704109	missense	probably damaging	1.00
R5250:Nrxn1	UTSW	17	90535441	intron	probably benign
R5473:Nrxn1	UTSW	17	90590092	missense	probably damaging	1.00
R5629:Nrxn1	UTSW	17	90590032	missense	possibly damaging	0.75
R5743:Nrxn1	UTSW	17	90643224	missense	probably damaging	1.00
R5910:Nrxn1	UTSW	17	90704318	nonsense	probably null
R5961:Nrxn1	UTSW	17	90454943	missense	probably damaging	0.99
R5992:Nrxn1	UTSW	17	90623507	missense	probably benign	0.01
R6024:Nrxn1	UTSW	17	90590098	missense	possibly damaging	0.88
R6031:Nrxn1	UTSW	17	90588790	missense	probably damaging	1.00
R6031:Nrxn1	UTSW	17	90588790	missense	probably damaging	1.00
R6185:Nrxn1	UTSW	17	90037136	missense	probably damaging	1.00
R6220:Nrxn1	UTSW	17	91088476	missense	probably benign	0.14
R6306:Nrxn1	UTSW	17	90565446	missense	possibly damaging	0.55
R6621:Nrxn1	UTSW	17	90162182	missense	probably damaging	1.00
R6669:Nrxn1	UTSW	17	90059563	missense	probably damaging	0.98
R6770:Nrxn1	UTSW	17	90037179	missense	probably damaging	1.00
R6798:Nrxn1	UTSW	17	90629950	missense	probably damaging	1.00
R6923:Nrxn1	UTSW	17	91088233	missense	probably benign	0.06
R7140:Nrxn1	UTSW	17	91088764	start gained	probably benign
R7374:Nrxn1	UTSW	17	90588669	critical splice donor site	probably null
R7564:Nrxn1	UTSW	17	90362906	missense	possibly damaging	0.64
R7570:Nrxn1	UTSW	17	90162379	missense	probably benign	0.35
R7800:Nrxn1	UTSW	17	91089207	unclassified	probably benign
R7828:Nrxn1	UTSW	17	90059551	missense	probably damaging	0.99
R7974:Nrxn1	UTSW	17	90700779	missense	probably damaging	1.00
R8001:Nrxn1	UTSW	17	91088536	missense	possibly damaging	0.49
R8189:Nrxn1	UTSW	17	90704209	missense	probably damaging	0.96
R8258:Nrxn1	UTSW	17	90163821	missense	probably damaging	0.99
R8259:Nrxn1	UTSW	17	90163821	missense	probably damaging	0.99
R8298:Nrxn1	UTSW	17	90704169	missense	probably damaging	1.00
R8801:Nrxn1	UTSW	17	90701965	critical splice donor site	probably benign
R8814:Nrxn1	UTSW	17	90630101	missense	probably damaging	1.00
R8873:Nrxn1	UTSW	17	90565393	nonsense	probably null
R8954:Nrxn1	UTSW	17	90590187	missense	probably damaging	1.00
R9086:Nrxn1	UTSW	17	90162364	missense	probably damaging	1.00
R9110:Nrxn1	UTSW	17	90561805	nonsense	probably null
R9498:Nrxn1	UTSW	17	90589969	missense	probably damaging	1.00
R9499:Nrxn1	UTSW	17	90630022	missense	probably damaging	1.00
R9552:Nrxn1	UTSW	17	90630022	missense	probably damaging	1.00
R9780:Nrxn1	UTSW	17	90623614	missense	possibly damaging	0.54
RF005:Nrxn1	UTSW	17	90362876	missense	probably damaging	1.00
RF024:Nrxn1	UTSW	17	90362876	missense	probably damaging	1.00
X0021:Nrxn1	UTSW	17	90590212	missense	probably damaging	1.00
X0063:Nrxn1	UTSW	17	90362831	missense	possibly damaging	0.54
Z1088:Nrxn1	UTSW	17	90059505	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACCTTGGCTGCAGTCCTTG -3'
(R):5'- TCGTGCTCTACTTCGACGAC -3'

Sequencing Primer
(F):5'- TCCTCGAGCAGTCGCATAC -3'
(R):5'- ACGAGGGCTTCTGCGACTTC -3'

Posted On

2017-06-26