|Institutional Source||Beutler Lab|
|Gene Name||potassium channel, subfamily K, member 3|
|Is this an essential gene?||Probably non essential (E-score: 0.115)|
|Stock #||R5982 (G1)|
|Chromosomal Location||30588170-30625271 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||G to T at 30622670 bp|
|Amino Acid Change||Valine to Leucine at position 355 (V355L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000098987 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000066295]|
|Predicted Effect||probably benign
AA Change: V355L
PolyPhen 2 Score 0.176 (Sensitivity: 0.92; Specificity: 0.87)
AA Change: V355L
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.0580|
|Coding Region Coverage||
|Validation Efficiency||98% (81/83)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene. [provided by RefSeq, Aug 2008]
PHENOTYPE: Mice homozygous for a null alleles exhibit decreased pH sensitivitive of action potential in serotonergic neurons. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Kcnk3||
(F):5'- TCATGACCATGAACGCCGAG -3'
(R):5'- TAGCCTGCACAGTTGGAGATTTAG -3'
(F):5'- ACCCAGTCACATGCGCTG -3'
(R):5'- TCACAGGTGCTCCAGGTCTG -3'