Mutagenetix > Incidental Mutations

Incidental Mutation 'R5985:Vmn2r25'

481598

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

044165-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R5985 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123822814-123853190 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123823628 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 585 (V585A)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

Predicted Effect

probably benign
Transcript: ENSMUST00000162046
AA Change: V585A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: V585A

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

96% (47/49)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130011E15Rik	A	T	19: 45,820,324	Y643N	probably damaging	Het
Abca13	A	C	11: 9,291,628	I1164L	probably benign	Het
Actn1	C	A	12: 80,168,395	G864V	probably damaging	Het
Adgrf1	T	C	17: 43,293,255	I34T	probably benign	Het
Ankrd10	T	A	8: 11,619,077	K225*	probably null	Het
Ankrd29	G	A	18: 12,279,775	A115V	probably damaging	Het
Atg2a	A	G	19: 6,254,637	T1175A	probably damaging	Het
Atp12a	A	G	14: 56,384,341	D781G	probably damaging	Het
B4galnt3	G	A	6: 120,210,158	P759S	probably damaging	Het
Card10	T	C	15: 78,791,211	I448V	probably benign	Het
Col15a1	T	C	4: 47,284,507	F821L	probably damaging	Het
Cpeb3	T	C	19: 37,087,552	Y498C	probably damaging	Het
Defb37	A	T	8: 18,986,331	C58S	probably damaging	Het
Faap20	T	C	4: 155,250,340		probably benign	Het
Frem1	C	T	4: 82,966,050	V1204I	probably benign	Het
Gal3st2	T	C	1: 93,873,613	V46A	possibly damaging	Het
Gan	C	T	8: 117,195,818	S430L	possibly damaging	Het
Gas2	T	A	7: 51,943,676	I168K	probably damaging	Het
Gli3	A	G	13: 15,723,555	D740G	probably damaging	Het
Gm15922	C	A	7: 3,737,317	G302C	probably damaging	Het
Gm3898	G	A	9: 43,830,032		noncoding transcript	Het
Gria4	T	C	9: 4,503,593	Q341R	probably damaging	Het
Herpud1	G	A	8: 94,390,794	R2Q	probably damaging	Het
Klf15	G	A	6: 90,466,721	G93R	possibly damaging	Het
Mtmr7	A	G	8: 40,551,832	F568L	probably benign	Het
Myod1	A	G	7: 46,377,798	Y229C	probably damaging	Het
Olfr1150-ps1	A	G	2: 87,357,605	I54V	probably benign	Het
Olfr1156	A	G	2: 87,949,321	M304T	probably benign	Het
Phf11b	A	G	14: 59,321,578	L235P	possibly damaging	Het
Plekha6	G	C	1: 133,272,307	R208P	possibly damaging	Het
Prox1	A	T	1: 190,146,955	F675L	possibly damaging	Het
Rnf144a	C	T	12: 26,317,780	E176K	probably benign	Het
Safb2	T	C	17: 56,563,181	E965G	possibly damaging	Het
Sik3	A	G	9: 46,211,675	N874S	probably damaging	Het
Slitrk3	T	C	3: 73,050,900	I180V	probably damaging	Het
Speg	T	C	1: 75,406,684	V1141A	possibly damaging	Het
Spns1	G	A	7: 126,376,730	T84I	probably benign	Het
Syne2	C	G	12: 75,966,159	P2709R	probably damaging	Het
Terb1	A	G	8: 104,482,316	S377P	probably damaging	Het
Terb1	A	T	8: 104,451,807	S662T	probably benign	Het
Tph1	A	G	7: 46,653,781	Y258H	probably damaging	Het
Trip12	T	A	1: 84,725,771	E1881D	probably damaging	Het
Trpc7	A	G	13: 56,810,545	L412P	probably damaging	Het
Ttll13	C	T	7: 80,254,638	A337V	probably damaging	Het
Ugt2b1	T	C	5: 86,919,668	K344E	possibly damaging	Het
Vmn1r36	T	A	6: 66,716,871	I7F	probably benign	Het
Vmn2r14	A	T	5: 109,220,216	N303K	possibly damaging	Het
Zan	A	C	5: 137,446,037		probably null	Het
Zfp423	A	T	8: 87,782,146	N502K	possibly damaging	Het
Zfp473	C	T	7: 44,733,328	R526Q	probably damaging	Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123853171	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123839365	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123853003	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123839429	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123839433	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123839764	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123823118	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123839598	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123823488	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123853025	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123828580	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123823622	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123823181	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123852049	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123839827	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123839522	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123853017	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123839323	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123851905	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123851795	splice site	probably null
R1780:Vmn2r25	UTSW	6	123828465	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123825378	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123839684	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123823295	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123839846	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123839559	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123853184	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123853085	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123840098	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123823647	missense	probably damaging	1.00
R4367:Vmn2r25	UTSW	6	123828537	missense	probably damaging	1.00
R4438:Vmn2r25	UTSW	6	123839797	missense	probably benign	0.03
R4488:Vmn2r25	UTSW	6	123822860	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123823023	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123853003	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123823223	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123828447	missense	probably benign
R5207:Vmn2r25	UTSW	6	123840103	missense	probably damaging	1.00
R5254:Vmn2r25	UTSW	6	123825318	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123828492	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123825296	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123828359	missense	possibly damaging	0.49
R6046:Vmn2r25	UTSW	6	123822917	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123822941	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123851982	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123823112	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123823610	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123828435	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123852081	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123823539	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123823142	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123823380	missense	probably damaging	0.99
R7802:Vmn2r25	UTSW	6	123851832	missense	possibly damaging	0.88
R7850:Vmn2r25	UTSW	6	123828472	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123823622	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123853017	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123853013	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123825391	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123823023	missense	possibly damaging	0.81
X0020:Vmn2r25	UTSW	6	123839400	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123822897	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAAGTGACTGTTCTAGGTTGG -3'
(R):5'- CCTTGAGAACTCAACAATTGCTCTG -3'

Sequencing Primer
(F):5'- GTTGGCCAATGAATATTAGTGAGC -3'
(R):5'- TGCTCTGCATAAAATATATGTGCC -3'

Posted On

2017-06-26