Mutagenetix > Incidental Mutation

Incidental Mutation 'R5988:Fat1'

481827

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

mFat1, Fath, 2310038E12Rik

MMRRC Submission

044168-MU

Accession Numbers

Genbank: NM_001081286

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5988 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

44935447-45052257 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 45029456 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 3051 (T3051A)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000098796
AA Change: T3028A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: T3028A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: T3028A

Predicted Effect

probably benign
Transcript: ENSMUST00000191428
AA Change: T3028A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: T3028A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000215588
AA Change: T3051A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Coding Region Coverage

1x: 99.8%
3x: 99.1%
10x: 95.3%
20x: 84.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	T	A	12: 55,377,217	M441K	probably damaging	Het
Actr8	T	A	14: 29,993,073	M615K	possibly damaging	Het
Acvr1c	A	T	2: 58,315,874	C25S	probably damaging	Het
Adk	A	T	14: 21,423,548	E291V	probably benign	Het
Ahnak	C	A	19: 9,009,347		probably benign	Het
Alg11	A	G	8: 22,062,028	T63A	probably benign	Het
Alox12	A	T	11: 70,251,587	S272T	probably benign	Het
Armc10	T	A	5: 21,660,583	S195T	probably damaging	Het
AW209491	C	T	13: 14,637,780	A406V	probably benign	Het
Baat	G	A	4: 49,502,871	P84S	probably damaging	Het
BB014433	G	A	8: 15,041,854	T333I	probably damaging	Het
BC035947	G	A	1: 78,499,206	R230*	probably null	Het
Bcr	G	A	10: 75,175,335	G26E	probably benign	Het
Ccdc88b	T	C	19: 6,855,980	E233G	probably damaging	Het
Cep295	A	G	9: 15,341,474	F353L	probably damaging	Het
Chd8	T	C	14: 52,217,938	M1031V	probably damaging	Het
Col17a1	C	G	19: 47,654,220	G885R	probably damaging	Het
Cyp11a1	A	C	9: 58,020,834	I214L	probably benign	Het
Cyp3a44	A	T	5: 145,794,918	S139R	probably damaging	Het
Ddhd2	G	A	8: 25,748,562	R64C	probably damaging	Het
Dll3	A	G	7: 28,294,112	W561R	probably damaging	Het
Dnajc3	T	C	14: 118,957,964	V97A	possibly damaging	Het
Drosha	C	T	15: 12,834,496		probably benign	Het
E2f8	T	C	7: 48,874,995	D302G	probably damaging	Het
Ecd	C	G	14: 20,324,561	D504H	probably damaging	Het
Eif4g2	C	A	7: 111,077,230	V331L	probably benign	Het
Fam124a	T	C	14: 62,587,537	I160T	possibly damaging	Het
Gm10271	A	T	10: 116,972,592	F6L	probably damaging	Het
Gm5039	T	A	12: 88,321,432	N17I	unknown	Het
Gm572	A	G	4: 148,668,507	E281G	possibly damaging	Het
Gm884	T	C	11: 103,615,896		probably benign	Het
Gm9944	T	C	4: 144,453,205		probably benign	Het
Gstm5	A	C	3: 107,895,954	M1L	probably benign	Het
Havcr1	A	G	11: 46,756,137	T133A	probably damaging	Het
Hey1	T	G	3: 8,666,319	S68R	probably damaging	Het
Hsf2bp	C	T	17: 32,011,175		probably null	Het
Id2	T	C	12: 25,095,724	N96S	probably benign	Het
Ifi206	A	C	1: 173,481,340	S363R	possibly damaging	Het
Il21r	T	C	7: 125,632,288	F296S	probably damaging	Het
Irgm2	A	G	11: 58,220,187	T247A	probably benign	Het
Irx5	T	A	8: 92,360,671	Y410*	probably null	Het
Kmt2c	A	G	5: 25,311,120	I2575T	probably benign	Het
Lmbrd2	T	A	15: 9,182,406		probably null	Het
Lrig1	A	C	6: 94,628,042	S191A	probably damaging	Het
Lypd6b	T	A	2: 49,946,166	C120S	probably damaging	Het
Mpdz	A	G	4: 81,284,575		probably null	Het
Myh6	G	T	14: 54,965,394	R23S	probably damaging	Het
Nat8f6	C	T	6: 85,809,058	M36I	probably benign	Het
Nol8	T	A	13: 49,672,614	M970K	possibly damaging	Het
Nrxn2	T	C	19: 6,492,871	F1042L	possibly damaging	Het
Nup160	T	C	2: 90,689,209	W277R	probably damaging	Het
Ogfr	A	T	2: 180,594,233	T204S	probably damaging	Het
Olfr136	T	C	17: 38,336,020	F288L	probably damaging	Het
Olfr16	T	A	1: 172,957,156	Y120*	probably null	Het
Olfr356	A	G	2: 36,937,224	Y35C	probably damaging	Het
Olfr59	T	C	11: 74,288,853	V69A	probably benign	Het
Olfr667	A	T	7: 104,916,918	V126E	probably damaging	Het
Olfr898	T	C	9: 38,349,749	V222A	probably benign	Het
Pcdh15	A	G	10: 74,379,357	I648V	probably benign	Het
Pced1b	T	A	15: 97,384,347	V89E	probably damaging	Het
Phax	G	T	18: 56,575,492	A43S	probably benign	Het
Phf20	A	G	2: 156,307,330	E976G	probably damaging	Het
Plin4	T	C	17: 56,109,567	T8A	probably benign	Het
Plxna1	G	A	6: 89,357,540	Q36*	probably null	Het
Pold1	T	C	7: 44,540,580	Y394C	probably damaging	Het
Ppp1r26	T	A	2: 28,452,554	V732E	probably benign	Het
Pramel5	A	T	4: 144,273,146	C124S	possibly damaging	Het
Prr14l	C	A	5: 32,830,851	L433F	probably damaging	Het
Ptpn14	A	G	1: 189,850,387	Y477C	probably damaging	Het
Rcl1	T	A	19: 29,121,767	I154N	probably damaging	Het
Rfpl4	T	C	7: 5,115,502	Y23C	probably damaging	Het
Rims1	T	C	1: 22,596,463	K146E	probably damaging	Het
Rims2	A	T	15: 39,292,182	K126M	probably damaging	Het
Rpusd4	T	A	9: 35,272,520		probably null	Het
Scn7a	C	T	2: 66,726,214	W342*	probably null	Het
Sh3pxd2a	A	G	19: 47,364,638	V128A	probably benign	Het
Slc15a5	T	C	6: 138,043,531	H268R	probably benign	Het
Slc22a3	T	C	17: 12,433,895	I321V	possibly damaging	Het
Slc7a6os	A	G	8: 106,202,333	S231P	probably benign	Het
Smad5	T	C	13: 56,735,985	S395P	probably damaging	Het
Sorbs3	G	A	14: 70,203,303	T20I	probably benign	Het
Spata9	A	G	13: 75,968,117	I42V	probably benign	Het
Srl	C	T	16: 4,523,028	C9Y	unknown	Het
Sugp2	T	C	8: 70,243,225	F283L	probably benign	Het
Syne2	T	C	12: 75,929,417		probably null	Het
Taar6	A	G	10: 23,985,256	Y131H	probably damaging	Het
Tango2	T	C	16: 18,302,690	T137A	probably damaging	Het
Tas2r106	T	A	6: 131,678,643	I82L	probably benign	Het
Tbc1d32	T	C	10: 56,088,337	E922G	probably damaging	Het
Tgfb2	G	T	1: 186,704,581	P65Q	probably benign	Het
Tjp2	T	G	19: 24,114,100	I566L	probably benign	Het
Tnrc6a	T	A	7: 123,182,380	N1424K	probably damaging	Het
Trim7	A	T	11: 48,837,686	M54L	probably benign	Het
Trpm1	T	A	7: 64,226,805	S535R	probably benign	Het
Tsc2	T	A	17: 24,620,766	N417I	probably damaging	Het
Ttn	T	C	2: 76,969,788	R359G	probably benign	Het
Vim	A	G	2: 13,582,485	T458A	probably benign	Het
Vps11	A	T	9: 44,353,924	S612R	probably benign	Het
Wdfy3	A	G	5: 101,884,138	S2273P	probably benign	Het
Wwox	G	T	8: 114,706,341	R249L	probably benign	Het
Zbtb4	G	T	11: 69,778,964	G838C	probably damaging	Het
Zkscan3	A	T	13: 21,396,291	C76S	probably damaging	Het
Znrf3	C	T	11: 5,281,776	C483Y	probably damaging	Het
Zscan2	A	G	7: 80,876,199	K556R	possibly damaging	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45024602	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	44951670	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45050940	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45033390	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45017857	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45024840	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45035532	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45023949	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45026841	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45026800	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45040576	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45051270	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45023049	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45029555	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45040700	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45036239	missense	probably benign	0.07
IGL01793:Fat1	APN	8	44989112	missense	probably benign
IGL01820:Fat1	APN	8	45010502	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	44952599	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45027540	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45023659	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	44950332	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	44950331	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45025818	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45040556	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	44951583	missense	probably benign	0.16
IGL02442:Fat1	APN	8	44950323	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45025072	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45051398	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45035591	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45023164	missense	probably benign
IGL02885:Fat1	APN	8	44989167	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45040682	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45024314	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45023614	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45030123	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	44950586	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	44950468	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45025241	missense	probably damaging	1.00
Laggardly	UTSW	8	45044464	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	44950371	missense	probably damaging	1.00
Shrinkage	UTSW	8	45018037	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45025480	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45024996	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45017434	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45029540	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45037207	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45024645	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45023790	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45024313	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	44951892	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45010123	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	44950348	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45024649	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45029534	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	44950542	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45022951	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	44951332	nonsense	probably null
R0624:Fat1	UTSW	8	45051168	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45026553	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45026749	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45040555	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45023983	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45018037	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45026598	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45033326	splice site	probably benign
R1051:Fat1	UTSW	8	45044506	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45039890	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45044279	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45010545	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45025622	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	44953244	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45033390	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45025484	nonsense	probably null
R1508:Fat1	UTSW	8	45026862	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45023383	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45018042	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45025178	nonsense	probably null
R1656:Fat1	UTSW	8	45025530	nonsense	probably null
R1662:Fat1	UTSW	8	44953164	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45036835	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45025576	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45024792	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45010482	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45036803	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	44953304	missense	probably benign	0.01
R1872:Fat1	UTSW	8	45038349	missense	probably damaging	1.00
R1883:Fat1	UTSW	8	45051147	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45023856	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45044228	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45033926	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45040682	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	44952393	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45023746	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45024332	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45026704	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45037463	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45024646	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45023700	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	44950371	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45040530	splice site	probably benign
R2416:Fat1	UTSW	8	45026383	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45044011	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45045173	splice site	probably null
R3109:Fat1	UTSW	8	45045173	splice site	probably null
R3196:Fat1	UTSW	8	44951868	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45017938	missense	probably benign
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	44953269	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45025479	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45023035	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45025481	missense	probably benign	0.09
R4078:Fat1	UTSW	8	44989122	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45036851	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45010437	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45050944	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45036787	missense	probably benign	0.00
R4364:Fat1	UTSW	8	44952962	missense	probably benign	0.01
R4394:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	44952346	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45023599	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45041894	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45026242	missense	probably benign
R4606:Fat1	UTSW	8	44950683	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45025147	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45033477	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45038326	missense	probably benign	0.04
R4824:Fat1	UTSW	8	44989114	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45036162	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45013065	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45012970	missense	probably benign	0.15
R4896:Fat1	UTSW	8	44951280	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45022963	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45036275	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45031263	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45023380	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45024282	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	44951814	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	44952512	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45025809	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45036131	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45025885	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45013053	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45036875	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45023479	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45039836	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	44953072	nonsense	probably null
R5734:Fat1	UTSW	8	45051209	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45017423	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45051129	missense	probably benign
R5886:Fat1	UTSW	8	45027681	critical splice donor site	probably null
R5886:Fat1	UTSW	8	45033395	missense	probably damaging	1.00
R5919:Fat1	UTSW	8	45026873	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45044036	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45033368	missense	probably damaging	1.00
R6166:Fat1	UTSW	8	44952485	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	44953392	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45027613	missense	probably damaging	0.99
R6351:Fat1	UTSW	8	45033495	missense	probably damaging	1.00
R6391:Fat1	UTSW	8	44952342	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	44950681	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6703:Fat1	UTSW	8	44953046	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45024373	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45026404	missense	probably damaging	1.00
R6852:Fat1	UTSW	8	45035598	missense	possibly damaging	0.46
R6863:Fat1	UTSW	8	45044464	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	44952452	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45051023	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45024495	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45043945	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	44953349	nonsense	probably null
R7062:Fat1	UTSW	8	44950216	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45040775	missense	probably benign	0.09
R7071:Fat1	UTSW	8	44989108	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45031468	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	44950925	missense	probably benign
R7210:Fat1	UTSW	8	45023503	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45010609	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45037438	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45026665	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	44952474	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45044152	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45031274	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45023160	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45036184	missense	probably damaging	1.00
R7526:Fat1	UTSW	8	45023427	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	44988994	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45037165	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45009850	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	44988930	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	44951633	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45023322	missense	probably damaging	1.00
R7762:Fat1	UTSW	8	45037337	missense	probably damaging	0.99
R7782:Fat1	UTSW	8	44950911	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45042223	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45041973	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	44950224	missense	probably benign
R7869:Fat1	UTSW	8	45051222	missense	probably benign	0.02
R8034:Fat1	UTSW	8	44951691	missense	probably benign	0.28
R8094:Fat1	UTSW	8	44952702	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45026058	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45039956	missense	probably null
R8221:Fat1	UTSW	8	44953353	missense
R8233:Fat1	UTSW	8	44952018	missense
R8250:Fat1	UTSW	8	44953299	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45030347	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45024169	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45040563	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45030313	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45023121	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45042294	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45031295	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45044550	nonsense	probably null
R9032:Fat1	UTSW	8	45039857	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45039901	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45013090	missense	possibly damaging	0.76
R9083:Fat1	UTSW	8	45038299	missense	probably benign	0.00
R9103:Fat1	UTSW	8	44951813	missense	probably benign	0.38
R9124:Fat1	UTSW	8	44950326	missense	probably benign
R9124:Fat1	UTSW	8	45025027	missense	possibly damaging	0.48
R9128:Fat1	UTSW	8	45009841	missense	probably benign	0.14
R9148:Fat1	UTSW	8	44952645	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	44951315	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	44951754	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45035477	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45010461	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	44953023	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45023191	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	44953038	missense	probably damaging	0.99
R9712:Fat1	UTSW	8	45017380	missense	probably benign	0.05
R9756:Fat1	UTSW	8	45043937	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	44988966	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45025734	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45023807	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	44950598	missense	probably benign
Z1176:Fat1	UTSW	8	45023596	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	45036838	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGGTACCTGTTACAAGGCTC -3'
(R):5'- TCATGTCTCCCACACAGAGG -3'

Sequencing Primer
(F):5'- CCTTGTTCCCACATCTGTTTTATAAG -3'
(R):5'- ATTAAGTTAGTCCCAAATTCCATCC -3'

Posted On

2017-06-26