Mutagenetix > Incidental Mutation

Incidental Mutation 'R5990:Rev3l'

481954

Institutional Source

Beutler Lab

Gene Symbol

Rev3l

Ensembl Gene

ENSMUSG00000019841

Gene Name

REV3 like, DNA directed polymerase zeta catalytic subunit

Synonyms

Sez4, Rev

MMRRC Submission

044170-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5990 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39732118-39875211 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 39823811 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 1435 (S1435A)

Ref Sequence

ENSEMBL: ENSMUSP00000131519 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019986] [ENSMUST00000131186] [ENSMUST00000139803] [ENSMUST00000164763]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000019986
AA Change: S1435A

PolyPhen 2 Score 0.168 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000019986
Gene: ENSMUSG00000019841
AA Change: S1435A

Domain	Start	End	E-Value	Type
Pfam:DNA_pol_B_exo1	43	201	1.6e-10	PFAM
low complexity region	494	506	N/A	INTRINSIC
low complexity region	959	969	N/A	INTRINSIC
low complexity region	1042	1057	N/A	INTRINSIC
low complexity region	1205	1216	N/A	INTRINSIC
low complexity region	1424	1440	N/A	INTRINSIC
low complexity region	1569	1595	N/A	INTRINSIC
Blast:POLBc	1825	2243	1e-163	BLAST
PDB:4GK5\|D	1863	1895	4e-13	PDB
POLBc	2308	2783	5.32e-105	SMART
Blast:POLBc	2860	2926	2e-14	BLAST
Pfam:zf-C4pol	3034	3103	8.2e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000131186

Predicted Effect

probably benign
Transcript: ENSMUST00000139803

SMART Domains

Protein: ENSMUSP00000115630
Gene: ENSMUSG00000019841

Domain	Start	End	E-Value	Type
Blast:POLBc	1	369	1e-155	BLAST
POLBc	434	805	4.77e-34	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145333

Predicted Effect

probably benign
Transcript: ENSMUST00000164763
AA Change: S1435A

PolyPhen 2 Score 0.168 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000131519
Gene: ENSMUSG00000019841
AA Change: S1435A

Domain	Start	End	E-Value	Type
Pfam:DNA_pol_B_exo1	43	200	1.3e-11	PFAM
low complexity region	494	506	N/A	INTRINSIC
Pfam:DUF4683	745	1132	1.7e-162	PFAM
low complexity region	1205	1216	N/A	INTRINSIC
low complexity region	1424	1440	N/A	INTRINSIC
low complexity region	1569	1595	N/A	INTRINSIC
Blast:POLBc	1825	2243	1e-163	BLAST
PDB:4GK5\|D	1863	1895	4e-13	PDB
POLBc	2308	2783	5.32e-105	SMART
Blast:POLBc	2860	2926	2e-14	BLAST
Pfam:zf-C4pol	3034	3102	6.1e-15	PFAM

Meta Mutation Damage Score

0.0648

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.5%
20x: 92.2%

Validation Efficiency

99% (77/78)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene represents the catalytic subunit of DNA polymerase zeta, which functions in translesion DNA synthesis. The encoded protein can be found in mitochondria, where it protects DNA from damage. Defects in this gene are a cause of Mobius syndrome. [provided by RefSeq, Jan 2017]
PHENOTYPE: Nullizygous mice exhibit complete embryonic lethality and abnormal embryonic tissue morphology with widespread degeneration and cell death. Mice carrying the amino acid substitution of phenylalanine for leucine at position 2610 display alterations in somatic hypermutation frequency and specificity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610028H24Rik	A	T	10: 76,449,289 (GRCm38)	M11L	probably benign	Het
Acad10	A	G	5: 121,645,405 (GRCm38)	L319P	probably damaging	Het
Adam12	C	A	7: 133,931,736 (GRCm38)	C471F	probably damaging	Het
Arfgef1	A	G	1: 10,172,921 (GRCm38)	Y1065H	probably damaging	Het
Atp8b3	G	A	10: 80,525,697 (GRCm38)	T797M	possibly damaging	Het
Auts2	G	A	5: 131,476,895 (GRCm38)		probably benign	Het
AW209491	C	T	13: 14,637,780 (GRCm38)	A406V	probably benign	Het
Ccdc146	A	G	5: 21,318,182 (GRCm38)	S286P	probably benign	Het
Chsy3	GT	G	18: 59,176,166 (GRCm38)	163	probably null	Het
Cmtr1	T	C	17: 29,702,161 (GRCm38)	Y794H	probably benign	Het
Copb2	A	G	9: 98,570,325 (GRCm38)	E54G	probably damaging	Het
Ctsk	A	T	3: 95,501,456 (GRCm38)	H77L	probably damaging	Het
Dnah3	T	C	7: 120,073,541 (GRCm38)	Y546C	probably benign	Het
Ephb2	A	T	4: 136,696,055 (GRCm38)	V304E	probably benign	Het
Ern1	C	T	11: 106,411,769 (GRCm38)	V420I	probably benign	Het
Esrrg	A	T	1: 188,198,798 (GRCm38)	E339V	probably damaging	Het
Fbxo10	A	C	4: 45,061,960 (GRCm38)	F189V	probably damaging	Het
Foxl1	G	T	8: 121,128,421 (GRCm38)	A154S	probably damaging	Het
Frs3	A	G	17: 47,701,677 (GRCm38)	D103G	possibly damaging	Het
Gdpd4	A	G	7: 98,040,930 (GRCm38)	T610A	probably benign	Het
Gm10271	A	T	10: 116,972,592 (GRCm38)	F6L	probably damaging	Het
Gm4847	C	T	1: 166,643,373 (GRCm38)	S36N	probably benign	Het
Grm8	G	T	6: 27,363,624 (GRCm38)	L631I	probably damaging	Het
Ints9	T	A	14: 65,039,328 (GRCm38)	L648Q	probably damaging	Het
Kansl1l	T	C	1: 66,735,726 (GRCm38)	H647R	probably damaging	Het
Kcnq1	T	A	7: 143,261,368 (GRCm38)	H501Q	probably damaging	Het
Kctd20	T	C	17: 28,966,910 (GRCm38)	L409P	probably benign	Het
Kiss1r	A	G	10: 79,918,707 (GRCm38)	T12A	probably benign	Het
Kndc1	T	A	7: 139,927,420 (GRCm38)	V1173E	probably damaging	Het
Kprp	T	C	3: 92,824,774 (GRCm38)	E323G	probably damaging	Het
Krtap10-4	A	T	10: 77,826,607 (GRCm38)		probably benign	Het
Lrrc37a	A	G	11: 103,500,958 (GRCm38)	Y1214H	probably benign	Het
Lysmd3	G	A	13: 81,669,588 (GRCm38)	G228D	probably damaging	Het
Muc16	G	A	9: 18,659,243 (GRCm38)	A660V	unknown	Het
Muc5b	T	C	7: 141,858,161 (GRCm38)	C1615R	unknown	Het
Nans	A	T	4: 46,489,441 (GRCm38)	N28I	probably damaging	Het
Nlrp4b	C	T	7: 10,714,491 (GRCm38)	S207L	possibly damaging	Het
Nrd1	T	G	4: 109,019,071 (GRCm38)	F355V	probably damaging	Het
Nrip2	C	T	6: 128,400,016 (GRCm38)		probably benign	Het
Nufip1	C	T	14: 76,114,188 (GRCm38)	P161L	probably damaging	Het
Ogdhl	C	T	14: 32,327,114 (GRCm38)	H114Y	possibly damaging	Het
Olfr1295	T	A	2: 111,564,674 (GRCm38)	I257F	probably damaging	Het
Olfr160	G	T	9: 37,712,110 (GRCm38)	H56Q	probably damaging	Het
Opa1	T	A	16: 29,587,018 (GRCm38)	W134R	probably damaging	Het
Parp14	G	A	16: 35,841,457 (GRCm38)	P1403S	probably benign	Het
Patl2	T	C	2: 122,124,484 (GRCm38)	D361G	probably damaging	Het
Pcx	A	G	19: 4,621,266 (GRCm38)	D1172G	probably damaging	Het
Phax	A	G	18: 56,575,603 (GRCm38)	T58A	probably benign	Het
Phf11b	T	A	14: 59,324,926 (GRCm38)	I177L	possibly damaging	Het
Pole	T	A	5: 110,302,144 (GRCm38)	V819D	probably damaging	Het
Poll	T	C	19: 45,553,155 (GRCm38)	D458G	possibly damaging	Het
Polr2m	G	A	9: 71,479,320 (GRCm38)		probably null	Het
Ppp1r9a	C	A	6: 5,134,660 (GRCm38)	H928N	probably benign	Het
Prdm2	T	C	4: 143,170,113 (GRCm38)	N102D	probably damaging	Het
Rbm45	A	T	2: 76,370,412 (GRCm38)	D95V	probably benign	Het
Rdh19	A	G	10: 127,859,594 (GRCm38)	M226V	probably benign	Het
Rgs20	A	G	1: 4,912,330 (GRCm38)	I305T	probably benign	Het
Rhobtb2	T	C	14: 69,796,369 (GRCm38)	N469S	probably damaging	Het
Rif1	C	G	2: 52,095,844 (GRCm38)	L614V	probably damaging	Het
Rps6ka1	A	G	4: 133,866,397 (GRCm38)	I177T	probably damaging	Het
Samd12	T	A	15: 53,719,623 (GRCm38)	D105V	probably damaging	Het
Setd3	A	T	12: 108,160,335 (GRCm38)	D88E	probably benign	Het
Sfmbt2	G	T	2: 10,579,381 (GRCm38)	V850L	possibly damaging	Het
Slc26a10	C	A	10: 127,178,758 (GRCm38)	A195S	possibly damaging	Het
Smcp	C	A	3: 92,584,250 (GRCm38)	A97S	unknown	Het
Stxbp5	G	A	10: 9,835,933 (GRCm38)	H248Y	probably damaging	Het
Syne2	T	C	12: 76,024,144 (GRCm38)	L4457P	probably benign	Het
Tbx3	A	G	5: 119,680,529 (GRCm38)	T390A	probably benign	Het
Tmeff2	G	A	1: 50,979,442 (GRCm38)	W194*	probably null	Het
Tmem120b	G	A	5: 123,104,481 (GRCm38)	R174Q	probably damaging	Het
Trio	A	G	15: 27,891,459 (GRCm38)	V402A	probably benign	Het
Ttll2	C	A	17: 7,352,367 (GRCm38)	G54W	possibly damaging	Het
Uba7	G	A	9: 107,981,234 (GRCm38)	V786M	probably damaging	Het
Vmn2r32	T	C	7: 7,479,810 (GRCm38)	E55G	probably damaging	Het
Wdr35	A	G	12: 9,016,511 (GRCm38)	D724G	probably damaging	Het
Xrcc1	G	A	7: 24,567,868 (GRCm38)	V381M	probably damaging	Het
Zdhhc11	T	A	13: 73,979,184 (GRCm38)	W227R	probably benign	Het
Zfp341	T	C	2: 154,645,659 (GRCm38)	S681P	probably damaging	Het
Zfp735	T	A	11: 73,690,348 (GRCm38)	D70E	possibly damaging	Het
Zmat4	G	A	8: 23,929,263 (GRCm38)	A104T	probably damaging	Het

Other mutations in Rev3l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Rev3l	APN	10	39,806,969 (GRCm38)	missense	probably benign
IGL00815:Rev3l	APN	10	39,859,153 (GRCm38)	missense	possibly damaging	0.79
IGL00964:Rev3l	APN	10	39,864,806 (GRCm38)	missense	probably benign	0.39
IGL01765:Rev3l	APN	10	39,828,265 (GRCm38)	missense	probably benign	0.00
IGL01792:Rev3l	APN	10	39,823,340 (GRCm38)	missense	probably benign
IGL01950:Rev3l	APN	10	39,821,157 (GRCm38)	missense	probably damaging	1.00
IGL01963:Rev3l	APN	10	39,822,737 (GRCm38)	missense	possibly damaging	0.90
IGL02089:Rev3l	APN	10	39,825,099 (GRCm38)	missense	probably damaging	1.00
IGL02288:Rev3l	APN	10	39,828,216 (GRCm38)	missense	probably benign
IGL02381:Rev3l	APN	10	39,821,346 (GRCm38)	missense	possibly damaging	0.83
IGL02409:Rev3l	APN	10	39,821,148 (GRCm38)	missense	possibly damaging	0.75
IGL02434:Rev3l	APN	10	39,822,591 (GRCm38)	missense	probably damaging	1.00
IGL02570:Rev3l	APN	10	39,848,013 (GRCm38)	missense	possibly damaging	0.68
IGL02581:Rev3l	APN	10	39,821,281 (GRCm38)	missense	probably benign	0.10
IGL02654:Rev3l	APN	10	39,862,734 (GRCm38)	missense	probably damaging	1.00
IGL02720:Rev3l	APN	10	39,822,395 (GRCm38)	nonsense	probably null
IGL02746:Rev3l	APN	10	39,824,589 (GRCm38)	missense	probably damaging	0.99
IGL02829:Rev3l	APN	10	39,825,240 (GRCm38)	missense	probably damaging	1.00
IGL02961:Rev3l	APN	10	39,827,945 (GRCm38)	missense	possibly damaging	0.65
IGL02974:Rev3l	APN	10	39,862,747 (GRCm38)	nonsense	probably null
IGL03029:Rev3l	APN	10	39,828,486 (GRCm38)	missense	probably benign	0.34
IGL03153:Rev3l	APN	10	39,806,878 (GRCm38)	missense	probably damaging	1.00
IGL03172:Rev3l	APN	10	39,824,790 (GRCm38)	missense	probably benign	0.10
R0068:Rev3l	UTSW	10	39,824,831 (GRCm38)	missense	possibly damaging	0.68
R0068:Rev3l	UTSW	10	39,824,831 (GRCm38)	missense	possibly damaging	0.68
R0153:Rev3l	UTSW	10	39,874,128 (GRCm38)	nonsense	probably null
R0308:Rev3l	UTSW	10	39,824,894 (GRCm38)	missense	probably benign	0.09
R0355:Rev3l	UTSW	10	39,817,286 (GRCm38)	missense	probably damaging	1.00
R0513:Rev3l	UTSW	10	39,828,143 (GRCm38)	missense	probably benign	0.00
R0523:Rev3l	UTSW	10	39,848,049 (GRCm38)	missense	probably benign	0.02
R0559:Rev3l	UTSW	10	39,824,487 (GRCm38)	missense	probably damaging	1.00
R0761:Rev3l	UTSW	10	39,874,195 (GRCm38)	missense	probably benign	0.32
R1023:Rev3l	UTSW	10	39,832,639 (GRCm38)	missense	probably damaging	1.00
R1159:Rev3l	UTSW	10	39,851,925 (GRCm38)	nonsense	probably null
R1398:Rev3l	UTSW	10	39,821,583 (GRCm38)	missense	probably benign	0.05
R1478:Rev3l	UTSW	10	39,783,333 (GRCm38)	critical splice donor site	probably null
R1517:Rev3l	UTSW	10	39,838,443 (GRCm38)	missense	probably benign	0.34
R1527:Rev3l	UTSW	10	39,822,822 (GRCm38)	missense	probably damaging	1.00
R1635:Rev3l	UTSW	10	39,806,662 (GRCm38)	missense	probably damaging	0.98
R1695:Rev3l	UTSW	10	39,824,616 (GRCm38)	missense	probably damaging	0.97
R1695:Rev3l	UTSW	10	39,824,615 (GRCm38)	nonsense	probably null
R1782:Rev3l	UTSW	10	39,799,885 (GRCm38)	missense	probably benign
R1815:Rev3l	UTSW	10	39,822,871 (GRCm38)	missense	probably benign	0.41
R1818:Rev3l	UTSW	10	39,828,424 (GRCm38)	missense	probably benign	0.05
R2039:Rev3l	UTSW	10	39,824,444 (GRCm38)	missense	probably damaging	1.00
R2071:Rev3l	UTSW	10	39,824,353 (GRCm38)	missense	probably benign	0.17
R2101:Rev3l	UTSW	10	39,828,096 (GRCm38)	missense	probably benign	0.00
R2141:Rev3l	UTSW	10	39,848,049 (GRCm38)	missense	probably benign	0.02
R2883:Rev3l	UTSW	10	39,825,156 (GRCm38)	missense	probably damaging	1.00
R3787:Rev3l	UTSW	10	39,846,210 (GRCm38)	missense	probably damaging	0.97
R3910:Rev3l	UTSW	10	39,820,556 (GRCm38)	missense	probably damaging	1.00
R3912:Rev3l	UTSW	10	39,820,556 (GRCm38)	missense	probably damaging	1.00
R3913:Rev3l	UTSW	10	39,820,556 (GRCm38)	missense	probably damaging	1.00
R4590:Rev3l	UTSW	10	39,806,933 (GRCm38)	missense	probably damaging	1.00
R4631:Rev3l	UTSW	10	39,828,416 (GRCm38)	missense	probably benign	0.44
R4633:Rev3l	UTSW	10	39,846,186 (GRCm38)	missense	probably damaging	1.00
R4707:Rev3l	UTSW	10	39,823,397 (GRCm38)	missense	probably damaging	0.99
R4724:Rev3l	UTSW	10	39,846,806 (GRCm38)	nonsense	probably null
R4810:Rev3l	UTSW	10	39,823,725 (GRCm38)	missense	probably benign	0.01
R4857:Rev3l	UTSW	10	39,838,459 (GRCm38)	missense	probably damaging	1.00
R4882:Rev3l	UTSW	10	39,821,460 (GRCm38)	missense	possibly damaging	0.89
R4928:Rev3l	UTSW	10	39,823,985 (GRCm38)	missense	probably benign	0.30
R4970:Rev3l	UTSW	10	39,823,330 (GRCm38)	missense	probably benign	0.00
R4977:Rev3l	UTSW	10	39,823,578 (GRCm38)	missense	possibly damaging	0.80
R5112:Rev3l	UTSW	10	39,823,330 (GRCm38)	missense	probably benign	0.00
R5261:Rev3l	UTSW	10	39,846,729 (GRCm38)	missense	probably damaging	1.00
R5419:Rev3l	UTSW	10	39,824,931 (GRCm38)	missense	possibly damaging	0.95
R5570:Rev3l	UTSW	10	39,852,075 (GRCm38)	critical splice donor site	probably null
R5628:Rev3l	UTSW	10	39,822,967 (GRCm38)	missense	probably damaging	0.98
R5689:Rev3l	UTSW	10	39,794,958 (GRCm38)	missense	probably damaging	1.00
R5781:Rev3l	UTSW	10	39,823,093 (GRCm38)	missense	probably benign	0.00
R5829:Rev3l	UTSW	10	39,806,906 (GRCm38)	missense	probably damaging	0.97
R5984:Rev3l	UTSW	10	39,742,689 (GRCm38)	intron	probably benign
R6054:Rev3l	UTSW	10	39,824,150 (GRCm38)	missense	probably benign	0.01
R6171:Rev3l	UTSW	10	39,862,713 (GRCm38)	nonsense	probably null
R6220:Rev3l	UTSW	10	39,822,779 (GRCm38)	missense	probably damaging	1.00
R6520:Rev3l	UTSW	10	39,822,702 (GRCm38)	missense	probably benign	0.06
R6798:Rev3l	UTSW	10	39,854,763 (GRCm38)	missense	probably damaging	1.00
R6811:Rev3l	UTSW	10	39,830,921 (GRCm38)	nonsense	probably null
R6812:Rev3l	UTSW	10	39,823,548 (GRCm38)	missense	probably benign
R6904:Rev3l	UTSW	10	39,821,481 (GRCm38)	missense	probably benign
R6905:Rev3l	UTSW	10	39,817,327 (GRCm38)	missense	probably benign	0.18
R6938:Rev3l	UTSW	10	39,862,710 (GRCm38)	missense	probably damaging	1.00
R7037:Rev3l	UTSW	10	39,851,975 (GRCm38)	missense	probably damaging	1.00
R7124:Rev3l	UTSW	10	39,822,167 (GRCm38)	nonsense	probably null
R7286:Rev3l	UTSW	10	39,823,605 (GRCm38)	missense	probably damaging	0.99
R7385:Rev3l	UTSW	10	39,823,682 (GRCm38)	missense	probably benign	0.01
R7575:Rev3l	UTSW	10	39,821,445 (GRCm38)	missense	possibly damaging	0.56
R7596:Rev3l	UTSW	10	39,821,538 (GRCm38)	missense	probably damaging	1.00
R7597:Rev3l	UTSW	10	39,822,884 (GRCm38)	missense	probably damaging	1.00
R7670:Rev3l	UTSW	10	39,836,722 (GRCm38)	missense	probably benign	0.01
R7804:Rev3l	UTSW	10	39,823,485 (GRCm38)	missense	probably benign	0.34
R7818:Rev3l	UTSW	10	39,823,902 (GRCm38)	missense	possibly damaging	0.54
R7874:Rev3l	UTSW	10	39,822,495 (GRCm38)	missense	possibly damaging	0.72
R7991:Rev3l	UTSW	10	39,863,738 (GRCm38)	missense	possibly damaging	0.52
R8059:Rev3l	UTSW	10	39,843,495 (GRCm38)	missense	probably damaging	1.00
R8174:Rev3l	UTSW	10	39,859,115 (GRCm38)	missense	probably damaging	1.00
R8187:Rev3l	UTSW	10	39,806,697 (GRCm38)	missense	probably benign
R8299:Rev3l	UTSW	10	39,821,541 (GRCm38)	missense	probably benign	0.01
R8352:Rev3l	UTSW	10	39,822,903 (GRCm38)	missense	probably damaging	1.00
R8452:Rev3l	UTSW	10	39,822,903 (GRCm38)	missense	probably damaging	1.00
R8468:Rev3l	UTSW	10	39,827,991 (GRCm38)	missense	probably damaging	0.99
R8487:Rev3l	UTSW	10	39,806,848 (GRCm38)	missense	probably damaging	1.00
R8512:Rev3l	UTSW	10	39,821,538 (GRCm38)	missense	probably damaging	1.00
R8554:Rev3l	UTSW	10	39,806,842 (GRCm38)	missense	probably benign	0.12
R8702:Rev3l	UTSW	10	39,838,469 (GRCm38)	nonsense	probably null
R8848:Rev3l	UTSW	10	39,846,709 (GRCm38)	missense	probably damaging	0.99
R8857:Rev3l	UTSW	10	39,794,969 (GRCm38)	nonsense	probably null
R8870:Rev3l	UTSW	10	39,862,790 (GRCm38)	missense	probably damaging	1.00
R9094:Rev3l	UTSW	10	39,824,813 (GRCm38)	missense	probably benign
R9175:Rev3l	UTSW	10	39,854,768 (GRCm38)	missense	possibly damaging	0.83
R9286:Rev3l	UTSW	10	39,806,951 (GRCm38)	missense	possibly damaging	0.54
R9299:Rev3l	UTSW	10	39,848,003 (GRCm38)	missense	probably damaging	1.00
R9307:Rev3l	UTSW	10	39,817,153 (GRCm38)	missense	probably benign	0.01
R9337:Rev3l	UTSW	10	39,822,854 (GRCm38)	missense	probably benign	0.40
R9342:Rev3l	UTSW	10	39,821,462 (GRCm38)	missense	probably benign
R9389:Rev3l	UTSW	10	39,822,971 (GRCm38)	missense	possibly damaging	0.47
R9395:Rev3l	UTSW	10	39,859,223 (GRCm38)	critical splice donor site	probably null
R9458:Rev3l	UTSW	10	39,783,251 (GRCm38)	missense	probably damaging	1.00
R9481:Rev3l	UTSW	10	39,825,037 (GRCm38)	missense	probably benign
R9646:Rev3l	UTSW	10	39,822,444 (GRCm38)	missense	probably damaging	1.00
R9686:Rev3l	UTSW	10	39,867,388 (GRCm38)	missense	possibly damaging	0.67
X0022:Rev3l	UTSW	10	39,828,607 (GRCm38)	critical splice donor site	probably null
Z1088:Rev3l	UTSW	10	39,824,318 (GRCm38)	missense	probably benign	0.41

Predicted Primers

PCR Primer
(F):5'- GTCTCAGGTAGCACAGAGTACAC -3'
(R):5'- TTGCTTCTGACAATGACCTTTG -3'

Sequencing Primer
(F):5'- TAGCACAGAGTACACAGGTATGTTC -3'
(R):5'- GCTTCTGACAATGACCTTTGTTTTAC -3'

Posted On

2017-06-26