Mutagenetix > Incidental Mutation

Incidental Mutation 'R6083:Wnk1'

482296

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

Prkwnk1, 6430573H23Rik, EG406236, Hsn2

MMRRC Submission

044242-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6083 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

119900930-120015633 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 120014562 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 11 (G11D)

Ref Sequence

ENSEMBL: ENSMUSP00000145304 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

probably damaging
Transcript: ENSMUST00000060043
AA Change: G11D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: G11D

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000088644
AA Change: G11D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: G11D

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000088646
AA Change: G11D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: G11D

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102262

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

probably damaging
Transcript: ENSMUST00000177761
AA Change: G11D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: G11D

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000203030
AA Change: G11D

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: G11D

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

96% (76/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810021J22Rik	C	A	11: 58,769,677 (GRCm39)	P73Q	possibly damaging	Het
Aak1	A	T	6: 86,940,978 (GRCm39)	I591F	unknown	Het
Ace	C	A	11: 105,876,093 (GRCm39)	Y816*	probably null	Het
Ahnak2	A	G	12: 112,746,715 (GRCm39)	V1205A	probably benign	Het
Ahnak2	T	G	12: 112,746,589 (GRCm39)	Q1076P	probably benign	Het
Ap2a1	G	A	7: 44,557,175 (GRCm39)	R263W	probably damaging	Het
Arhgap29	A	G	3: 121,786,397 (GRCm39)	T257A	probably benign	Het
Bora	A	T	14: 99,299,730 (GRCm39)	Q234L	possibly damaging	Het
Ccdc17	A	T	4: 116,454,123 (GRCm39)	Q47L	possibly damaging	Het
Cep170	C	T	1: 176,602,191 (GRCm39)	R305H	probably damaging	Het
Cyb5r4	C	T	9: 86,939,221 (GRCm39)	P335S	probably damaging	Het
Cyp2c54	A	G	19: 40,062,206 (GRCm39)	L17P	probably benign	Het
Cyp2c69	A	T	19: 39,837,900 (GRCm39)	V394E	probably damaging	Het
Dedd2	G	A	7: 24,910,715 (GRCm39)	P154S	probably benign	Het
Defb36	A	T	2: 152,446,408 (GRCm39)	M1L	unknown	Het
Dnaja1	A	G	4: 40,731,713 (GRCm39)	D263G	probably benign	Het
Dock10	T	C	1: 80,510,148 (GRCm39)	N1560S	probably damaging	Het
Efnb2	T	C	8: 8,672,328 (GRCm39)		probably null	Het
Eif3e	A	G	15: 43,129,540 (GRCm39)	I196T	probably damaging	Het
Ercc4	T	A	16: 12,927,903 (GRCm39)	C24*	probably null	Het
Fmn1	A	G	2: 113,194,648 (GRCm39)	E116G	unknown	Het
Gnas	A	G	2: 174,139,655 (GRCm39)	M1V	probably null	Het
Grin2a	C	A	16: 9,397,404 (GRCm39)	M894I	probably benign	Het
Herc2	G	T	7: 55,878,253 (GRCm39)	S4566I	probably benign	Het
Hmcn1	G	A	1: 150,631,044 (GRCm39)	P918L	probably damaging	Het
Hmcn1	G	T	1: 150,631,045 (GRCm39)	P918T	probably damaging	Het
Hsd3b2	T	A	3: 98,619,372 (GRCm39)	Y191F	possibly damaging	Het
Ifit1bl2	A	C	19: 34,597,217 (GRCm39)	F133C	possibly damaging	Het
Itih2	T	C	2: 10,113,705 (GRCm39)		probably benign	Het
Itsn1	T	C	16: 91,649,899 (GRCm39)	L191P	probably benign	Het
Kdr	T	C	5: 76,105,026 (GRCm39)	K1068R	probably damaging	Het
Lmtk2	T	C	5: 144,119,574 (GRCm39)	L1345P	probably damaging	Het
Man2b2	T	A	5: 36,966,385 (GRCm39)	D936V	probably damaging	Het
Mapk1ip1	G	A	7: 138,438,317 (GRCm39)	R38*	probably null	Het
Med13l	T	C	5: 118,859,551 (GRCm39)	V246A	possibly damaging	Het
Mlec	T	A	5: 115,286,108 (GRCm39)	T248S	probably benign	Het
Mslnl	T	A	17: 25,956,876 (GRCm39)	V54D	possibly damaging	Het
Muc16	G	A	9: 18,568,508 (GRCm39)	T1337I	unknown	Het
Nek7	C	T	1: 138,443,392 (GRCm39)	S187N	probably damaging	Het
Neto2	A	G	8: 86,367,214 (GRCm39)	V538A	probably benign	Het
Nktr	T	C	9: 121,579,202 (GRCm39)		probably benign	Het
Npy6r	A	G	18: 44,409,559 (GRCm39)	K327E	probably damaging	Het
Or2ag1	A	G	7: 106,472,789 (GRCm39)	I221T	probably damaging	Het
Or3a1c	A	G	11: 74,046,396 (GRCm39)	M139V	possibly damaging	Het
Or4a72	T	C	2: 89,406,016 (GRCm39)	D18G	probably benign	Het
Or4c123	T	A	2: 89,127,368 (GRCm39)	D82V	probably damaging	Het
Or5b109	A	T	19: 13,211,889 (GRCm39)	I92F	probably benign	Het
Pcdhga4	A	T	18: 37,820,478 (GRCm39)	N676Y	probably damaging	Het
Pde2a	T	C	7: 101,152,086 (GRCm39)	I331T	possibly damaging	Het
Pip5k1b	A	T	19: 24,281,399 (GRCm39)	Y486*	probably null	Het
Plch2	A	G	4: 155,085,275 (GRCm39)	M272T	probably benign	Het
Rbm12	G	A	2: 155,939,646 (GRCm39)		probably benign	Het
Rgs19	T	C	2: 181,331,300 (GRCm39)	E111G	probably damaging	Het
Rhbdf1	T	C	11: 32,160,066 (GRCm39)	N145S	probably damaging	Het
Rnf4	T	A	5: 34,508,565 (GRCm39)		probably null	Het
Rpa1	T	C	11: 75,205,737 (GRCm39)	T207A	probably damaging	Het
Rufy4	A	C	1: 74,168,556 (GRCm39)	Q113P	probably damaging	Het
Sh3pxd2b	T	G	11: 32,372,985 (GRCm39)	S717R	probably benign	Het
Sin3a	A	T	9: 57,014,824 (GRCm39)	I682F	probably damaging	Het
Sipa1l2	A	T	8: 126,195,212 (GRCm39)	V842E	possibly damaging	Het
Slc5a4a	A	G	10: 75,983,431 (GRCm39)	I23V	unknown	Het
Slitrk5	A	G	14: 111,919,157 (GRCm39)	N927S	probably benign	Het
Smc6	A	G	12: 11,326,354 (GRCm39)	K117R	possibly damaging	Het
Sod2	G	A	17: 13,226,918 (GRCm39)		probably benign	Het
Stxbp1	A	G	2: 32,686,030 (GRCm39)	I567T	possibly damaging	Het
Tbkbp1	C	A	11: 97,038,206 (GRCm39)	L209F	probably damaging	Het
Tll1	G	T	8: 64,491,620 (GRCm39)		probably null	Het
Tmem38b	T	C	4: 53,840,765 (GRCm39)	L60S	probably damaging	Het
Trib1	G	A	15: 59,526,324 (GRCm39)	R298H	probably damaging	Het
Trim30b	A	G	7: 104,015,349 (GRCm39)	V13A	probably damaging	Het
Trip11	G	A	12: 101,856,001 (GRCm39)	T425I	probably benign	Het
Ttn	C	A	2: 76,720,317 (GRCm39)		probably benign	Het
Tymp	GC	GCC	15: 89,258,567 (GRCm39)		probably null	Het
Ush2a	T	A	1: 187,999,220 (GRCm39)	S177T	probably damaging	Het
Usp40	A	T	1: 87,906,281 (GRCm39)	S651R	probably benign	Het
Vmn1r125	T	C	7: 21,006,644 (GRCm39)	S181P	probably damaging	Het
Vmn1r198	T	A	13: 22,538,928 (GRCm39)	V138D	possibly damaging	Het
Vmn1r56	A	G	7: 5,199,317 (GRCm39)	L100P	probably damaging	Het
Vmn2r32	T	A	7: 7,467,209 (GRCm39)	D773V	probably benign	Het
Vmn2r53	A	C	7: 12,315,808 (GRCm39)	H670Q	probably benign	Het
Vmn2r69	A	T	7: 85,055,711 (GRCm39)	I809N	probably damaging	Het
Wdr7	G	A	18: 63,861,540 (GRCm39)	G184D	probably damaging	Het
Zfp110	A	C	7: 12,578,602 (GRCm39)	E171A	possibly damaging	Het
Zkscan6	T	C	11: 65,706,757 (GRCm39)	V134A	probably damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119,937,669 (GRCm39)	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119,914,372 (GRCm39)	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119,925,358 (GRCm39)	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119,940,446 (GRCm39)	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119,926,039 (GRCm39)	unclassified	probably benign
IGL02268:Wnk1	APN	6	119,914,334 (GRCm39)	nonsense	probably null
IGL02348:Wnk1	APN	6	119,940,289 (GRCm39)	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119,940,415 (GRCm39)	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119,914,823 (GRCm39)	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119,903,594 (GRCm39)	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119,931,148 (GRCm39)	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119,930,856 (GRCm39)	missense	probably benign	0.41
Actor	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
Bad	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
Blink	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
Knock	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
Narrow	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
nictitate	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
opportunity	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
path	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
Stormy	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
tear	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
Tic	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
Unforgiving	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
Window	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
Woke	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119,921,760 (GRCm39)	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119,940,328 (GRCm39)	nonsense	probably null
R0044:Wnk1	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119,929,694 (GRCm39)	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119,905,124 (GRCm39)	intron	probably benign
R0453:Wnk1	UTSW	6	119,940,112 (GRCm39)	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119,946,293 (GRCm39)	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119,939,764 (GRCm39)	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119,903,525 (GRCm39)	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119,928,953 (GRCm39)	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119,903,644 (GRCm39)	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119,925,466 (GRCm39)	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119,928,967 (GRCm39)	splice site	probably benign
R1188:Wnk1	UTSW	6	119,925,670 (GRCm39)	nonsense	probably null
R1245:Wnk1	UTSW	6	119,925,418 (GRCm39)	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119,927,645 (GRCm39)	splice site	probably benign
R1869:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119,929,884 (GRCm39)	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119,911,343 (GRCm39)	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119,914,539 (GRCm39)	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119,905,118 (GRCm39)	splice site	probably null
R2144:Wnk1	UTSW	6	119,925,949 (GRCm39)	unclassified	probably benign
R2186:Wnk1	UTSW	6	119,925,528 (GRCm39)	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119,940,601 (GRCm39)	splice site	probably null
R2338:Wnk1	UTSW	6	119,946,495 (GRCm39)	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119,913,328 (GRCm39)	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119,946,315 (GRCm39)	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119,926,012 (GRCm39)	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119,925,087 (GRCm39)	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119,926,222 (GRCm39)	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119,930,856 (GRCm39)	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119,903,387 (GRCm39)	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119,928,872 (GRCm39)	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119,942,705 (GRCm39)	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119,929,732 (GRCm39)	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119,939,399 (GRCm39)	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119,926,338 (GRCm39)	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5002:Wnk1	UTSW	6	119,914,924 (GRCm39)	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5152:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120,014,149 (GRCm39)	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119,945,484 (GRCm39)	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119,925,852 (GRCm39)	unclassified	probably benign
R5600:Wnk1	UTSW	6	119,926,319 (GRCm39)	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
R6110:Wnk1	UTSW	6	119,949,958 (GRCm39)	intron	probably benign
R6128:Wnk1	UTSW	6	119,940,747 (GRCm39)	splice site	probably null
R6237:Wnk1	UTSW	6	119,929,728 (GRCm39)	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119,925,546 (GRCm39)	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119,925,742 (GRCm39)	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
R7024:Wnk1	UTSW	6	119,942,687 (GRCm39)	intron	probably benign
R7072:Wnk1	UTSW	6	119,914,822 (GRCm39)	missense	unknown
R7087:Wnk1	UTSW	6	120,014,491 (GRCm39)	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119,925,268 (GRCm39)	missense	unknown
R7134:Wnk1	UTSW	6	119,903,389 (GRCm39)	missense	unknown
R7137:Wnk1	UTSW	6	120,015,173 (GRCm39)	unclassified	probably benign
R7142:Wnk1	UTSW	6	119,926,240 (GRCm39)	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
R7218:Wnk1	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
R7498:Wnk1	UTSW	6	119,904,157 (GRCm39)	missense	unknown
R7599:Wnk1	UTSW	6	119,906,789 (GRCm39)	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119,909,699 (GRCm39)	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119,926,137 (GRCm39)	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120,014,409 (GRCm39)	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119,909,675 (GRCm39)	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119,930,794 (GRCm39)	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119,940,454 (GRCm39)	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119,906,960 (GRCm39)	splice site	probably null
R8359:Wnk1	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119,911,388 (GRCm39)	missense	unknown
R8519:Wnk1	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120,013,959 (GRCm39)	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119,939,393 (GRCm39)	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119,925,631 (GRCm39)	missense	unknown
R9148:Wnk1	UTSW	6	119,925,631 (GRCm39)	missense	unknown
R9354:Wnk1	UTSW	6	119,942,660 (GRCm39)	missense	unknown
R9379:Wnk1	UTSW	6	119,928,678 (GRCm39)	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120,013,993 (GRCm39)	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119,925,166 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ATTGGAGTCGCAGATGACG -3'
(R):5'- TAGCCGTCTTCACTTGGGTC -3'

Posted On

2017-07-14