Mutagenetix > Incidental Mutation

Incidental Mutation 'R6083:Herc2'

482304

Institutional Source

Beutler Lab

Gene Symbol

Herc2

Ensembl Gene

ENSMUSG00000030451

Gene Name

HECT and RLD domain containing E3 ubiquitin protein ligase 2

Synonyms

D7H15F37S1, D7H15F32S1, rjs, jdf2, D15F32S1h

MMRRC Submission

044242-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.921) question?

Stock #

R6083 (G1)

Quality Score

181.009

Status

Validated

Chromosome

Chromosomal Location

56050196-56231800 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 56228505 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Isoleucine at position 4566 (S4566I)

Ref Sequence

ENSEMBL: ENSMUSP00000145997 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076226] [ENSMUST00000164095] [ENSMUST00000205303]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000076226
AA Change: S4602I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000075579
Gene: ENSMUSG00000030451
AA Change: S4602I

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	7.6e-16	PFAM
Pfam:RCC1_2	554	583	6e-9	PFAM
Pfam:RCC1	570	615	7.1e-17	PFAM
Pfam:RCC1_2	606	637	6.9e-8	PFAM
Pfam:RCC1	624	673	7.2e-15	PFAM
Pfam:RCC1	676	725	3.3e-18	PFAM
Pfam:RCC1_2	712	740	1.6e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1933	5.8e-29	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2633	2.6e-43	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	9.2e-8	PFAM
Pfam:RCC1	3066	3115	3.7e-17	PFAM
Pfam:RCC1_2	3102	3131	3.9e-11	PFAM
Pfam:RCC1	3118	3162	1.9e-15	PFAM
Pfam:RCC1	3172	3221	9.6e-15	PFAM
Pfam:RCC1_2	3208	3236	2.2e-7	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	5.1e-8	PFAM
Pfam:RCC1	4059	4108	1.5e-16	PFAM
Pfam:RCC1	4111	4155	7.9e-16	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	4.3e-10	PFAM
Pfam:RCC1	4269	4318	1.2e-17	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000164095
AA Change: S4602I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000131573
Gene: ENSMUSG00000030451
AA Change: S4602I

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	2.9e-15	PFAM
Pfam:RCC1_2	554	583	1.6e-8	PFAM
Pfam:RCC1	570	615	2.3e-16	PFAM
Pfam:RCC1	624	673	1.2e-14	PFAM
Pfam:RCC1_2	660	689	2.1e-7	PFAM
Pfam:RCC1	676	725	9.8e-18	PFAM
Pfam:RCC1_2	712	740	1.1e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1931	9.3e-25	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2632	1.4e-39	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	1.6e-7	PFAM
Pfam:RCC1	3066	3115	7.1e-16	PFAM
Pfam:RCC1_2	3102	3131	7.1e-11	PFAM
Pfam:RCC1	3118	3163	1.2e-14	PFAM
Pfam:RCC1	3172	3221	4.7e-15	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	1.2e-7	PFAM
Pfam:RCC1	4059	4108	9.6e-15	PFAM
Pfam:RCC1	4111	4156	5.6e-15	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	7.3e-10	PFAM
Pfam:RCC1	4269	4318	1.6e-16	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000205303
AA Change: S4566I

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000205678

Meta Mutation Damage Score

0.0595

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

96% (76/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. [provided by RefSeq, Mar 2012]
PHENOTYPE: Homozygotes for null mutations exhibit runting, nervousness, and incoordination. Males are sterile with sperm abnormalities, while females show reduced fertility and impaired maternal ability. Also see alleles at the Oca2 (p) locus for deletions that encompass the Herc2 gene. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810021J22Rik	C	A	11: 58,878,851	P73Q	possibly damaging	Het
Aak1	A	T	6: 86,963,996	I591F	unknown	Het
Ace	C	A	11: 105,985,267	Y816*	probably null	Het
Ahnak2	A	G	12: 112,782,612	V1205A	probably benign	Het
Ahnak2	T	G	12: 112,782,999	Q1076P	probably benign	Het
Ap2a1	G	A	7: 44,907,751	R263W	probably damaging	Het
Arhgap29	A	G	3: 121,992,748	T257A	probably benign	Het
Bora	A	T	14: 99,062,294	Q234L	possibly damaging	Het
Ccdc17	A	T	4: 116,596,926	Q47L	possibly damaging	Het
Cep170	C	T	1: 176,774,625	R305H	probably damaging	Het
Cyb5r4	C	T	9: 87,057,168	P335S	probably damaging	Het
Cyp2c54	A	G	19: 40,073,762	L17P	probably benign	Het
Cyp2c69	A	T	19: 39,849,456	V394E	probably damaging	Het
Dedd2	G	A	7: 25,211,290	P154S	probably benign	Het
Defb36	A	T	2: 152,604,488	M1L	unknown	Het
Dnaja1	A	G	4: 40,731,713	D263G	probably benign	Het
Dock10	T	C	1: 80,532,431	N1560S	probably damaging	Het
Efnb2	T	C	8: 8,622,328		probably null	Het
Eif3e	A	G	15: 43,266,144	I196T	probably damaging	Het
Ercc4	T	A	16: 13,110,039	C24*	probably null	Het
Fmn1	A	G	2: 113,364,303	E116G	unknown	Het
Gnas	A	G	2: 174,297,862	M1V	probably null	Het
Grin2a	C	A	16: 9,579,540	M894I	probably benign	Het
Hmcn1	G	T	1: 150,755,294	P918T	probably damaging	Het
Hmcn1	G	A	1: 150,755,293	P918L	probably damaging	Het
Hsd3b2	T	A	3: 98,712,056	Y191F	possibly damaging	Het
Ifit1bl2	A	C	19: 34,619,817	F133C	possibly damaging	Het
Itih2	T	C	2: 10,108,894		probably benign	Het
Itsn1	T	C	16: 91,853,011	L191P	probably benign	Het
Kdr	T	C	5: 75,944,366	K1068R	probably damaging	Het
Lmtk2	T	C	5: 144,182,756	L1345P	probably damaging	Het
Man2b2	T	A	5: 36,809,041	D936V	probably damaging	Het
Mapk1ip1	G	A	7: 138,836,588	R38*	probably null	Het
Med13l	T	C	5: 118,721,486	V246A	possibly damaging	Het
Mlec	T	A	5: 115,148,049	T248S	probably benign	Het
Mslnl	T	A	17: 25,737,902	V54D	possibly damaging	Het
Muc16	G	A	9: 18,657,212	T1337I	unknown	Het
Nek7	C	T	1: 138,515,654	S187N	probably damaging	Het
Neto2	A	G	8: 85,640,585	V538A	probably benign	Het
Nktr	T	C	9: 121,750,136		probably benign	Het
Npy6r	A	G	18: 44,276,492	K327E	probably damaging	Het
Olfr1230	T	A	2: 89,297,024	D82V	probably damaging	Het
Olfr1245	T	C	2: 89,575,672	D18G	probably benign	Het
Olfr1463	A	T	19: 13,234,525	I92F	probably benign	Het
Olfr402	A	G	11: 74,155,570	M139V	possibly damaging	Het
Olfr705	A	G	7: 106,873,582	I221T	probably damaging	Het
Pcdhga4	A	T	18: 37,687,425	N676Y	probably damaging	Het
Pde2a	T	C	7: 101,502,879	I331T	possibly damaging	Het
Pip5k1b	A	T	19: 24,304,035	Y486*	probably null	Het
Plch2	A	G	4: 155,000,818	M272T	probably benign	Het
Rbm12	G	A	2: 156,097,726		probably benign	Het
Rgs19	T	C	2: 181,689,507	E111G	probably damaging	Het
Rhbdf1	T	C	11: 32,210,066	N145S	probably damaging	Het
Rnf4	T	A	5: 34,351,221		probably null	Het
Rpa1	T	C	11: 75,314,911	T207A	probably damaging	Het
Rufy4	A	C	1: 74,129,397	Q113P	probably damaging	Het
Sh3pxd2b	T	G	11: 32,422,985	S717R	probably benign	Het
Sin3a	A	T	9: 57,107,540	I682F	probably damaging	Het
Sipa1l2	A	T	8: 125,468,473	V842E	possibly damaging	Het
Slc5a4a	A	G	10: 76,147,597	I23V	unknown	Het
Slitrk5	A	G	14: 111,681,725	N927S	probably benign	Het
Smc6	A	G	12: 11,276,353	K117R	possibly damaging	Het
Sod2	G	A	17: 13,008,031		probably benign	Het
Stxbp1	A	G	2: 32,796,018	I567T	possibly damaging	Het
Tbkbp1	C	A	11: 97,147,380	L209F	probably damaging	Het
Tll1	G	T	8: 64,038,586		probably null	Het
Tmem38b	T	C	4: 53,840,765	L60S	probably damaging	Het
Trib1	G	A	15: 59,654,475	R298H	probably damaging	Het
Trim30b	A	G	7: 104,366,142	V13A	probably damaging	Het
Trip11	G	A	12: 101,889,742	T425I	probably benign	Het
Ttn	C	A	2: 76,889,973		probably benign	Het
Tymp	GC	GCC	15: 89,374,364		probably null	Het
Ush2a	T	A	1: 188,267,023	S177T	probably damaging	Het
Usp40	A	T	1: 87,978,559	S651R	probably benign	Het
Vmn1r125	T	C	7: 21,272,719	S181P	probably damaging	Het
Vmn1r198	T	A	13: 22,354,758	V138D	possibly damaging	Het
Vmn1r56	A	G	7: 5,196,318	L100P	probably damaging	Het
Vmn2r32	T	A	7: 7,464,210	D773V	probably benign	Het
Vmn2r53	A	C	7: 12,581,881	H670Q	probably benign	Het
Vmn2r69	A	T	7: 85,406,503	I809N	probably damaging	Het
Wdr7	G	A	18: 63,728,469	G184D	probably damaging	Het
Wnk1	C	T	6: 120,037,601	G11D	probably damaging	Het
Zfp110	A	C	7: 12,844,675	E171A	possibly damaging	Het
Zkscan6	T	C	11: 65,815,931	V134A	probably damaging	Het

Other mutations in Herc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Herc2	APN	7	56,124,299 (GRCm38)	missense	probably damaging	1.00
IGL00529:Herc2	APN	7	56,157,753 (GRCm38)	missense	probably benign
IGL00548:Herc2	APN	7	56,206,565 (GRCm38)	missense	probably benign	0.20
IGL00970:Herc2	APN	7	56,181,064 (GRCm38)	splice site	probably benign
IGL01141:Herc2	APN	7	56,212,841 (GRCm38)	missense	possibly damaging	0.47
IGL01147:Herc2	APN	7	56,156,949 (GRCm38)	missense	probably benign	0.43
IGL01150:Herc2	APN	7	56,181,133 (GRCm38)	missense	probably damaging	1.00
IGL01519:Herc2	APN	7	56,103,950 (GRCm38)	missense	probably damaging	1.00
IGL01576:Herc2	APN	7	56,226,661 (GRCm38)	critical splice donor site	probably null
IGL01626:Herc2	APN	7	56,085,142 (GRCm38)	missense	probably benign	0.02
IGL01658:Herc2	APN	7	56,159,452 (GRCm38)	missense	probably damaging	1.00
IGL01707:Herc2	APN	7	56,165,187 (GRCm38)	missense	probably damaging	1.00
IGL01727:Herc2	APN	7	56,137,806 (GRCm38)	missense	probably damaging	1.00
IGL01935:Herc2	APN	7	56,153,793 (GRCm38)	missense	probably benign
IGL01969:Herc2	APN	7	56,185,831 (GRCm38)	splice site	probably benign
IGL02074:Herc2	APN	7	56,087,444 (GRCm38)	splice site	probably benign
IGL02261:Herc2	APN	7	56,206,744 (GRCm38)	missense	probably damaging	0.99
IGL02339:Herc2	APN	7	56,121,722 (GRCm38)	missense	probably benign	0.01
IGL02353:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02360:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02409:Herc2	APN	7	56,220,469 (GRCm38)	splice site	probably null
IGL02528:Herc2	APN	7	56,108,893 (GRCm38)	splice site	probably benign
IGL02571:Herc2	APN	7	56,153,386 (GRCm38)	missense	probably damaging	1.00
IGL02578:Herc2	APN	7	56,106,535 (GRCm38)	splice site	probably null
IGL02661:Herc2	APN	7	56,113,073 (GRCm38)	missense	probably damaging	1.00
IGL02664:Herc2	APN	7	56,135,678 (GRCm38)	nonsense	probably null
IGL02675:Herc2	APN	7	56,164,101 (GRCm38)	missense	probably damaging	0.99
IGL02689:Herc2	APN	7	56,165,283 (GRCm38)	splice site	probably benign
IGL02710:Herc2	APN	7	56,137,814 (GRCm38)	missense	possibly damaging	0.95
IGL02750:Herc2	APN	7	56,204,379 (GRCm38)	splice site	probably benign
IGL02754:Herc2	APN	7	56,097,498 (GRCm38)	missense	probably damaging	1.00
IGL03029:Herc2	APN	7	56,168,967 (GRCm38)	missense	probably damaging	1.00
IGL03039:Herc2	APN	7	56,169,021 (GRCm38)	splice site	probably benign
IGL03082:Herc2	APN	7	56,185,923 (GRCm38)	missense	probably benign	0.19
IGL03090:Herc2	APN	7	56,204,473 (GRCm38)	missense	probably damaging	0.96
IGL03154:Herc2	APN	7	56,202,159 (GRCm38)	missense	probably damaging	1.00
IGL03165:Herc2	APN	7	56,191,912 (GRCm38)	missense	probably damaging	1.00
IGL03201:Herc2	APN	7	56,219,768 (GRCm38)	missense	probably damaging	1.00
IGL03234:Herc2	APN	7	56,103,862 (GRCm38)	missense	probably damaging	1.00
IGL03293:Herc2	APN	7	56,155,130 (GRCm38)	missense	probably benign	0.43
IGL03331:Herc2	APN	7	56,135,267 (GRCm38)	splice site	probably benign
IGL03340:Herc2	APN	7	56,090,920 (GRCm38)	missense	possibly damaging	0.51
IGL03409:Herc2	APN	7	56,228,569 (GRCm38)	missense	probably damaging	1.00
alarmed	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
hyper	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0798_herc2_487	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R1370_Herc2_948	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R2030_Herc2_144	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
uptight	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
I0000:Herc2	UTSW	7	56,136,729 (GRCm38)	splice site	probably benign
PIT1430001:Herc2	UTSW	7	56,226,954 (GRCm38)	missense	probably damaging	1.00
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0058:Herc2	UTSW	7	56,170,483 (GRCm38)	missense	possibly damaging	0.93
R0114:Herc2	UTSW	7	56,153,774 (GRCm38)	splice site	probably benign
R0117:Herc2	UTSW	7	56,213,611 (GRCm38)	splice site	probably benign
R0141:Herc2	UTSW	7	56,121,561 (GRCm38)	missense	probably benign	0.17
R0266:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0401:Herc2	UTSW	7	56,157,732 (GRCm38)	missense	probably damaging	0.99
R0403:Herc2	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0437:Herc2	UTSW	7	56,219,815 (GRCm38)	nonsense	probably null
R0491:Herc2	UTSW	7	56,122,366 (GRCm38)	missense	possibly damaging	0.54
R0499:Herc2	UTSW	7	56,184,369 (GRCm38)	nonsense	probably null
R0580:Herc2	UTSW	7	56,138,791 (GRCm38)	missense	probably damaging	1.00
R0650:Herc2	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
R0744:Herc2	UTSW	7	56,206,036 (GRCm38)	splice site	probably benign
R0798:Herc2	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R0842:Herc2	UTSW	7	56,121,705 (GRCm38)	missense	probably benign
R0849:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0850:Herc2	UTSW	7	56,204,483 (GRCm38)	missense	probably benign	0.09
R0926:Herc2	UTSW	7	56,132,548 (GRCm38)	missense	possibly damaging	0.67
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1292:Herc2	UTSW	7	56,197,203 (GRCm38)	missense	probably benign	0.05
R1370:Herc2	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R1443:Herc2	UTSW	7	56,204,733 (GRCm38)	missense	possibly damaging	0.69
R1445:Herc2	UTSW	7	56,168,996 (GRCm38)	missense	probably damaging	1.00
R1541:Herc2	UTSW	7	56,135,657 (GRCm38)	missense	probably damaging	1.00
R1550:Herc2	UTSW	7	56,135,658 (GRCm38)	missense	probably damaging	1.00
R1551:Herc2	UTSW	7	56,146,669 (GRCm38)	missense	probably benign	0.01
R1633:Herc2	UTSW	7	56,229,369 (GRCm38)	missense	probably null	1.00
R1635:Herc2	UTSW	7	56,136,667 (GRCm38)	missense	probably benign	0.00
R1659:Herc2	UTSW	7	56,135,105 (GRCm38)	missense	probably benign	0.00
R1682:Herc2	UTSW	7	56,088,400 (GRCm38)	missense	possibly damaging	0.87
R1697:Herc2	UTSW	7	56,153,905 (GRCm38)	missense	probably benign	0.43
R1748:Herc2	UTSW	7	56,148,823 (GRCm38)	critical splice donor site	probably null
R1802:Herc2	UTSW	7	56,184,332 (GRCm38)	missense	probably damaging	1.00
R1835:Herc2	UTSW	7	56,206,765 (GRCm38)	nonsense	probably null
R1836:Herc2	UTSW	7	56,155,105 (GRCm38)	nonsense	probably null
R1872:Herc2	UTSW	7	56,157,509 (GRCm38)	missense	probably benign	0.18
R1889:Herc2	UTSW	7	56,189,813 (GRCm38)	missense	possibly damaging	0.60
R1906:Herc2	UTSW	7	56,114,864 (GRCm38)	missense	probably benign	0.01
R2004:Herc2	UTSW	7	56,137,859 (GRCm38)	missense	probably damaging	1.00
R2030:Herc2	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
R2037:Herc2	UTSW	7	56,205,961 (GRCm38)	missense	probably damaging	1.00
R2059:Herc2	UTSW	7	56,163,897 (GRCm38)	missense	probably damaging	1.00
R2068:Herc2	UTSW	7	56,132,497 (GRCm38)	missense	probably damaging	1.00
R2072:Herc2	UTSW	7	56,226,964 (GRCm38)	missense	probably damaging	1.00
R2085:Herc2	UTSW	7	56,212,965 (GRCm38)	missense	possibly damaging	0.94
R2115:Herc2	UTSW	7	56,185,828 (GRCm38)	splice site	probably benign
R2160:Herc2	UTSW	7	56,212,922 (GRCm38)	missense	probably benign	0.00
R2173:Herc2	UTSW	7	56,185,951 (GRCm38)	missense	probably benign	0.27
R2221:Herc2	UTSW	7	56,169,018 (GRCm38)	critical splice donor site	probably null
R2280:Herc2	UTSW	7	56,137,271 (GRCm38)	missense	possibly damaging	0.79
R3078:Herc2	UTSW	7	56,137,243 (GRCm38)	missense	probably benign
R3104:Herc2	UTSW	7	56,135,355 (GRCm38)	missense	probably benign	0.23
R3177:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3277:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3766:Herc2	UTSW	7	56,163,824 (GRCm38)	missense	probably damaging	1.00
R3770:Herc2	UTSW	7	56,165,007 (GRCm38)	missense	probably benign
R3807:Herc2	UTSW	7	56,207,809 (GRCm38)	missense	probably damaging	1.00
R3912:Herc2	UTSW	7	56,098,437 (GRCm38)	missense	probably damaging	0.98
R4004:Herc2	UTSW	7	56,106,465 (GRCm38)	missense	possibly damaging	0.53
R4039:Herc2	UTSW	7	56,156,411 (GRCm38)	missense	probably damaging	0.98
R4190:Herc2	UTSW	7	56,122,448 (GRCm38)	missense	probably benign	0.03
R4225:Herc2	UTSW	7	56,164,987 (GRCm38)	missense	probably damaging	1.00
R4334:Herc2	UTSW	7	56,226,654 (GRCm38)	missense	probably damaging	1.00
R4405:Herc2	UTSW	7	56,170,477 (GRCm38)	missense	probably damaging	1.00
R4448:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4450:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4565:Herc2	UTSW	7	56,153,838 (GRCm38)	missense	possibly damaging	0.71
R4667:Herc2	UTSW	7	56,131,253 (GRCm38)	missense	probably damaging	1.00
R4747:Herc2	UTSW	7	56,106,393 (GRCm38)	missense	possibly damaging	0.80
R4762:Herc2	UTSW	7	56,170,640 (GRCm38)	missense	probably benign	0.19
R4829:Herc2	UTSW	7	56,106,492 (GRCm38)	missense	probably benign	0.39
R4832:Herc2	UTSW	7	56,098,417 (GRCm38)	nonsense	probably null
R4895:Herc2	UTSW	7	56,222,986 (GRCm38)	missense	probably damaging	1.00
R4904:Herc2	UTSW	7	56,157,486 (GRCm38)	missense	probably damaging	0.99
R4908:Herc2	UTSW	7	56,177,912 (GRCm38)	missense	probably benign	0.01
R4911:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4921:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
R4939:Herc2	UTSW	7	56,206,736 (GRCm38)	missense	probably damaging	1.00
R5155:Herc2	UTSW	7	56,227,826 (GRCm38)	missense	possibly damaging	0.85
R5184:Herc2	UTSW	7	56,122,351 (GRCm38)	missense	probably damaging	1.00
R5269:Herc2	UTSW	7	56,168,870 (GRCm38)	nonsense	probably null
R5306:Herc2	UTSW	7	56,184,961 (GRCm38)	missense	probably damaging	1.00
R5314:Herc2	UTSW	7	56,219,786 (GRCm38)	missense	probably damaging	0.99
R5369:Herc2	UTSW	7	56,182,700 (GRCm38)	missense	probably damaging	1.00
R5418:Herc2	UTSW	7	56,137,565 (GRCm38)	missense	probably damaging	1.00
R5420:Herc2	UTSW	7	56,203,830 (GRCm38)	missense	probably damaging	0.96
R5463:Herc2	UTSW	7	56,194,262 (GRCm38)	missense	probably damaging	1.00
R5510:Herc2	UTSW	7	56,206,771 (GRCm38)	missense	probably damaging	1.00
R5634:Herc2	UTSW	7	56,206,783 (GRCm38)	missense	probably damaging	1.00
R5638:Herc2	UTSW	7	56,204,416 (GRCm38)	missense	probably benign	0.01
R5690:Herc2	UTSW	7	56,157,705 (GRCm38)	missense	probably benign
R5762:Herc2	UTSW	7	56,197,190 (GRCm38)	missense	possibly damaging	0.68
R5807:Herc2	UTSW	7	56,230,919 (GRCm38)	missense	probably damaging	0.99
R5878:Herc2	UTSW	7	56,124,248 (GRCm38)	missense	probably benign
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6192:Herc2	UTSW	7	56,207,762 (GRCm38)	missense	probably damaging	1.00
R6193:Herc2	UTSW	7	56,156,901 (GRCm38)	missense	probably damaging	0.98
R6261:Herc2	UTSW	7	56,197,072 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,153,166 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,204,718 (GRCm38)	missense	possibly damaging	0.51
R6298:Herc2	UTSW	7	56,191,265 (GRCm38)	missense	probably benign
R6299:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R6326:Herc2	UTSW	7	56,222,934 (GRCm38)	missense	probably damaging	0.98
R6347:Herc2	UTSW	7	56,194,403 (GRCm38)	critical splice donor site	probably null
R6394:Herc2	UTSW	7	56,215,981 (GRCm38)	missense	probably damaging	1.00
R6500:Herc2	UTSW	7	56,146,645 (GRCm38)	nonsense	probably null
R6526:Herc2	UTSW	7	56,157,330 (GRCm38)	missense	probably damaging	0.99
R6592:Herc2	UTSW	7	56,207,690 (GRCm38)	critical splice acceptor site	probably null
R6619:Herc2	UTSW	7	56,068,092 (GRCm38)	nonsense	probably null
R6719:Herc2	UTSW	7	56,212,826 (GRCm38)	missense	probably damaging	1.00
R6750:Herc2	UTSW	7	56,097,447 (GRCm38)	missense	probably damaging	1.00
R6807:Herc2	UTSW	7	56,164,922 (GRCm38)	missense	probably damaging	1.00
R6811:Herc2	UTSW	7	56,113,433 (GRCm38)	nonsense	probably null
R6837:Herc2	UTSW	7	56,189,841 (GRCm38)	missense	possibly damaging	0.89
R6838:Herc2	UTSW	7	56,108,778 (GRCm38)	missense	probably damaging	1.00
R6902:Herc2	UTSW	7	56,135,486 (GRCm38)	missense	probably benign	0.37
R6983:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6985:Herc2	UTSW	7	56,132,480 (GRCm38)	missense	probably damaging	1.00
R6985:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6986:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6987:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R7113:Herc2	UTSW	7	56,203,849 (GRCm38)	missense	probably damaging	0.99
R7173:Herc2	UTSW	7	56,203,827 (GRCm38)	missense	probably damaging	1.00
R7202:Herc2	UTSW	7	56,131,286 (GRCm38)	missense	probably damaging	0.99
R7205:Herc2	UTSW	7	56,182,640 (GRCm38)	missense	probably damaging	1.00
R7236:Herc2	UTSW	7	56,085,080 (GRCm38)	missense	probably benign	0.29
R7297:Herc2	UTSW	7	56,136,658 (GRCm38)	missense	probably benign	0.00
R7358:Herc2	UTSW	7	56,182,675 (GRCm38)	missense	possibly damaging	0.48
R7438:Herc2	UTSW	7	56,103,718 (GRCm38)	splice site	probably null
R7537:Herc2	UTSW	7	56,219,779 (GRCm38)	nonsense	probably null
R7578:Herc2	UTSW	7	56,134,800 (GRCm38)	missense	probably benign	0.07
R7614:Herc2	UTSW	7	56,153,275 (GRCm38)	nonsense	probably null
R7638:Herc2	UTSW	7	56,157,438 (GRCm38)	missense	probably benign	0.26
R7638:Herc2	UTSW	7	56,220,525 (GRCm38)	missense	probably damaging	1.00
R7646:Herc2	UTSW	7	56,134,613 (GRCm38)	missense	probably benign
R7663:Herc2	UTSW	7	56,136,685 (GRCm38)	missense	probably benign
R7665:Herc2	UTSW	7	56,153,155 (GRCm38)	missense	probably damaging	1.00
R7691:Herc2	UTSW	7	56,191,845 (GRCm38)	missense	probably benign
R7733:Herc2	UTSW	7	56,188,664 (GRCm38)	missense	probably damaging	0.99
R7767:Herc2	UTSW	7	56,228,527 (GRCm38)	missense	probably benign	0.39
R7802:Herc2	UTSW	7	56,164,090 (GRCm38)	missense	probably damaging	1.00
R7847:Herc2	UTSW	7	56,157,560 (GRCm38)	critical splice donor site	probably null
R7956:Herc2	UTSW	7	56,113,400 (GRCm38)	missense	probably damaging	0.97
R7985:Herc2	UTSW	7	56,165,244 (GRCm38)	missense	probably benign
R8003:Herc2	UTSW	7	56,168,904 (GRCm38)	missense	possibly damaging	0.94
R8045:Herc2	UTSW	7	56,184,900 (GRCm38)	missense	probably damaging	1.00
R8085:Herc2	UTSW	7	56,229,679 (GRCm38)	missense	probably benign	0.01
R8134:Herc2	UTSW	7	56,085,136 (GRCm38)	missense	probably benign	0.10
R8259:Herc2	UTSW	7	56,205,890 (GRCm38)	missense	probably damaging	0.99
R8286:Herc2	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
R8304:Herc2	UTSW	7	56,159,438 (GRCm38)	missense	probably damaging	1.00
R8321:Herc2	UTSW	7	56,229,348 (GRCm38)	missense	possibly damaging	0.84
R8332:Herc2	UTSW	7	56,146,595 (GRCm38)	missense	probably damaging	1.00
R8432:Herc2	UTSW	7	56,155,112 (GRCm38)	missense	probably benign	0.14
R8516:Herc2	UTSW	7	56,206,570 (GRCm38)	missense	probably benign	0.05
R8676:Herc2	UTSW	7	56,188,613 (GRCm38)	missense	probably damaging	1.00
R8738:Herc2	UTSW	7	56,148,654 (GRCm38)	missense	possibly damaging	0.78
R8742:Herc2	UTSW	7	56,094,395 (GRCm38)	missense	probably benign	0.12
R8796:Herc2	UTSW	7	56,135,375 (GRCm38)	missense	probably benign	0.01
R8825:Herc2	UTSW	7	56,050,878 (GRCm38)	start codon destroyed	probably null	0.01
R8826:Herc2	UTSW	7	56,106,396 (GRCm38)	missense	probably benign	0.12
R8842:Herc2	UTSW	7	56,088,311 (GRCm38)	missense	probably damaging	0.99
R9103:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R9124:Herc2	UTSW	7	56,184,308 (GRCm38)	missense	probably damaging	1.00
R9134:Herc2	UTSW	7	56,182,429 (GRCm38)	missense	probably damaging	0.99
R9168:Herc2	UTSW	7	56,152,460 (GRCm38)	missense	probably damaging	0.99
R9173:Herc2	UTSW	7	56,206,602 (GRCm38)	missense	probably damaging	0.97
R9238:Herc2	UTSW	7	56,163,760 (GRCm38)	missense	probably damaging	0.98
R9249:Herc2	UTSW	7	56,113,142 (GRCm38)	missense	probably damaging	1.00
R9344:Herc2	UTSW	7	56,122,364 (GRCm38)	missense	probably benign	0.07
R9432:Herc2	UTSW	7	56,131,184 (GRCm38)	missense	probably damaging	1.00
R9472:Herc2	UTSW	7	56,164,095 (GRCm38)	missense	probably damaging	1.00
R9513:Herc2	UTSW	7	56,113,100 (GRCm38)	missense	probably damaging	1.00
R9579:Herc2	UTSW	7	56,108,752 (GRCm38)	missense	probably damaging	0.99
R9596:Herc2	UTSW	7	56,184,847 (GRCm38)	missense
R9664:Herc2	UTSW	7	56,170,590 (GRCm38)	missense	possibly damaging	0.90
R9760:Herc2	UTSW	7	56,163,911 (GRCm38)	critical splice donor site	probably null
R9781:Herc2	UTSW	7	56,100,348 (GRCm38)	missense	possibly damaging	0.53
RF024:Herc2	UTSW	7	56,226,525 (GRCm38)	missense	probably damaging	1.00
X0011:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
X0023:Herc2	UTSW	7	56,090,918 (GRCm38)	missense	possibly damaging	0.73
X0057:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
X0064:Herc2	UTSW	7	56,191,258 (GRCm38)	missense	probably benign
X0064:Herc2	UTSW	7	56,191,211 (GRCm38)	missense	probably benign	0.01
Z1088:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1088:Herc2	UTSW	7	56,087,341 (GRCm38)	missense	probably benign	0.00
Z1088:Herc2	UTSW	7	56,226,589 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,432 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,381 (GRCm38)	missense	possibly damaging	0.86
Z1176:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1176:Herc2	UTSW	7	56,097,533 (GRCm38)	missense	possibly damaging	0.48
Z1176:Herc2	UTSW	7	56,132,498 (GRCm38)	missense	probably damaging	1.00
Z1177:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1177:Herc2	UTSW	7	56,121,589 (GRCm38)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- TAGCTCCTTGGGAATAGTACTTCC -3'
(R):5'- TGGGAATAAGCACTTTGAACAC -3'

Posted On

2017-07-14