Incidental Mutation 'R6086:Asic4'
ID 482456
Institutional Source Beutler Lab
Gene Symbol Asic4
Ensembl Gene ENSMUSG00000033007
Gene Name acid-sensing (proton-gated) ion channel family member 4
MMRRC Submission 044427-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6086 (G1)
Quality Score 225.009
Status Validated
Chromosome 1
Chromosomal Location 75450436-75474343 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) G to A at 75473243 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Valine to Isoleucine at position 468 (V468I)
Ref Sequence ENSEMBL: ENSMUSP00000045598 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000037708] [ENSMUST00000079205] [ENSMUST00000094818] [ENSMUST00000113577] [ENSMUST00000124042] [ENSMUST00000138683]
AlphaFold Q7TNS7
Predicted Effect possibly damaging
Transcript: ENSMUST00000037708
AA Change: V468I

PolyPhen 2 Score 0.644 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000045598
Gene: ENSMUSG00000033007
AA Change: V468I

low complexity region 12 26 N/A INTRINSIC
Pfam:ASC 45 464 5.3e-92 PFAM
low complexity region 507 533 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000079205
SMART Domains Protein: ENSMUSP00000078199
Gene: ENSMUSG00000032997

low complexity region 37 46 N/A INTRINSIC
Pfam:CHGN 262 761 5e-150 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000094818
SMART Domains Protein: ENSMUSP00000092412
Gene: ENSMUSG00000032997

Pfam:CHGN 100 599 3.3e-174 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000113577
AA Change: V449I

PolyPhen 2 Score 0.587 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000109207
Gene: ENSMUSG00000033007
AA Change: V449I

low complexity region 12 26 N/A INTRINSIC
Pfam:ASC 45 346 5.5e-94 PFAM
Pfam:ASC 344 446 1.4e-42 PFAM
low complexity region 488 514 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000124042
SMART Domains Protein: ENSMUSP00000122057
Gene: ENSMUSG00000032997

low complexity region 37 46 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000138683
SMART Domains Protein: ENSMUSP00000117253
Gene: ENSMUSG00000032997

low complexity region 37 46 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000143496
Predicted Effect noncoding transcript
Transcript: ENSMUST00000194916
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.2%
  • 20x: 94.9%
Validation Efficiency 96% (65/68)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. [provided by RefSeq, Feb 2012]
PHENOTYPE: Homozygous knockout increases the fear response in the predator-odor induced fear test and increases anxiety in open field and elevated plus maze tests. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3110062M04Rik A G 6: 34,874,620 (GRCm38) S127P probably damaging Het
Apob G T 12: 8,015,164 (GRCm38) K4044N probably benign Het
Atf4 T A 15: 80,257,453 (GRCm38) V348D probably benign Het
Bcl9 A G 3: 97,205,524 (GRCm38) V1205A possibly damaging Het
Bora A T 14: 99,062,294 (GRCm38) Q234L possibly damaging Het
Cap2 C A 13: 46,635,712 (GRCm38) P131Q probably damaging Het
Cdyl2 A G 8: 116,589,296 (GRCm38) S318P probably damaging Het
Ces1a T G 8: 93,027,353 (GRCm38) N341H probably benign Het
Crlf3 C T 11: 80,048,610 (GRCm38) V352M possibly damaging Het
Cybb C G X: 9,450,750 (GRCm38) D246H probably benign Het
Dnah2 T C 11: 69,516,008 (GRCm38) T529A probably benign Het
Dnah9 A C 11: 66,085,174 (GRCm38) S1350A probably benign Het
Dnah9 C T 11: 65,989,915 (GRCm38) D2619N probably damaging Het
Dnajc6 C T 4: 101,597,807 (GRCm38) S65L probably benign Het
Dnm3 C A 1: 162,321,033 (GRCm38) R256S probably damaging Het
Enpp2 T C 15: 54,845,834 (GRCm38) D795G probably damaging Het
Fah A T 7: 84,588,912 (GRCm38) W367R probably damaging Het
Fam220a T A 5: 143,563,041 (GRCm38) H69Q probably benign Het
Fgd3 T A 13: 49,287,296 (GRCm38) T220S probably benign Het
Furin G T 7: 80,395,431 (GRCm38) H248Q probably damaging Het
Gabrg1 A T 5: 70,754,053 (GRCm38) L410Q probably damaging Het
Gm10801 AAGT AAGTAGT 2: 98,663,803 (GRCm38) probably null Het
Gm9742 T C 13: 8,030,033 (GRCm38) noncoding transcript Het
Gpcpd1 A G 2: 132,538,114 (GRCm38) S252P probably damaging Het
Hnrnpdl C T 5: 100,036,481 (GRCm38) G398S probably null Het
Hspa1l T C 17: 34,978,155 (GRCm38) V390A possibly damaging Het
Htr3b T C 9: 48,947,298 (GRCm38) S94G probably benign Het
Klf10 G T 15: 38,296,937 (GRCm38) S271R probably benign Het
Klk1b3 T A 7: 44,201,734 (GRCm38) L197Q probably damaging Het
Knl1 A G 2: 119,094,068 (GRCm38) R1861G probably damaging Het
Lexm T C 4: 106,613,206 (GRCm38) E218G probably damaging Het
Myo9a C T 9: 59,790,057 (GRCm38) Q374* probably null Het
Myt1l G A 12: 29,832,332 (GRCm38) G509R unknown Het
Ncapg T C 5: 45,693,236 (GRCm38) L728P probably damaging Het
Nfxl1 A C 5: 72,541,019 (GRCm38) F228V probably benign Het
Oc90 A G 15: 65,889,711 (GRCm38) S153P probably damaging Het
Olfr1230 A G 2: 89,296,854 (GRCm38) C139R probably damaging Het
Olfr1458 A G 19: 13,102,381 (GRCm38) *308Q probably null Het
Pbk A T 14: 65,815,253 (GRCm38) K182* probably null Het
Piezo1 A G 8: 122,501,657 (GRCm38) F296S possibly damaging Het
Psmg1 T A 16: 95,980,044 (GRCm38) Y288F probably damaging Het
Rab11fip4 G A 11: 79,683,480 (GRCm38) D132N probably damaging Het
Recql4 T C 15: 76,704,587 (GRCm38) D1051G probably damaging Het
Reep5 A C 18: 34,357,131 (GRCm38) D104E probably damaging Het
Rnpepl1 T C 1: 92,917,681 (GRCm38) Y441H probably damaging Het
Scnm1 T C 3: 95,130,285 (GRCm38) I157V probably benign Het
Sema3b T C 9: 107,600,848 (GRCm38) D446G probably damaging Het
Sema4d C T 13: 51,713,745 (GRCm38) R190Q probably damaging Het
Slco3a1 G A 7: 74,318,590 (GRCm38) R461C possibly damaging Het
Tcrg-C3 T C 13: 19,263,284 (GRCm38) S136P probably damaging Het
Tenm2 T A 11: 36,008,646 (GRCm38) I2562F possibly damaging Het
Thegl T A 5: 77,061,305 (GRCm38) V458E probably benign Het
Tmem40 G T 6: 115,733,667 (GRCm38) N120K possibly damaging Het
Tmod3 T C 9: 75,500,123 (GRCm38) H351R probably benign Het
Tnn C T 1: 160,086,120 (GRCm38) V1268M probably damaging Het
Tpmt T A 13: 47,035,030 (GRCm38) D132V probably damaging Het
Trib1 G A 15: 59,654,475 (GRCm38) R298H probably damaging Het
Tsen2 A G 6: 115,560,075 (GRCm38) E264G probably benign Het
Urah A G 7: 140,836,798 (GRCm38) I60M probably benign Het
Vmn2r26 T A 6: 124,039,560 (GRCm38) S328T possibly damaging Het
Vmn2r27 G C 6: 124,191,999 (GRCm38) T724R probably damaging Het
Washc2 A G 6: 116,256,216 (GRCm38) probably null Het
Wdyhv1 C A 15: 58,150,628 (GRCm38) A71E probably damaging Het
Zfp955b T A 17: 33,302,504 (GRCm38) W316R probably benign Het
Zranb2 T C 3: 157,543,246 (GRCm38) probably null Het
Other mutations in Asic4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01399:Asic4 APN 1 75,469,146 (GRCm38) missense possibly damaging 0.94
IGL01470:Asic4 APN 1 75,450,866 (GRCm38) missense probably damaging 1.00
IGL02645:Asic4 APN 1 75,473,354 (GRCm38) unclassified probably benign
IGL03002:Asic4 APN 1 75,451,323 (GRCm38) missense possibly damaging 0.61
positron UTSW 1 75,473,043 (GRCm38) nonsense probably null
PIT4445001:Asic4 UTSW 1 75,451,127 (GRCm38) missense probably benign 0.03
R0106:Asic4 UTSW 1 75,451,127 (GRCm38) missense probably benign 0.03
R0138:Asic4 UTSW 1 75,469,687 (GRCm38) missense possibly damaging 0.87
R0453:Asic4 UTSW 1 75,473,511 (GRCm38) unclassified probably benign
R0573:Asic4 UTSW 1 75,469,102 (GRCm38) splice site probably benign
R0705:Asic4 UTSW 1 75,451,370 (GRCm38) missense probably damaging 1.00
R1892:Asic4 UTSW 1 75,469,482 (GRCm38) missense probably damaging 1.00
R1912:Asic4 UTSW 1 75,469,232 (GRCm38) missense possibly damaging 0.93
R3614:Asic4 UTSW 1 75,473,058 (GRCm38) missense probably damaging 1.00
R3790:Asic4 UTSW 1 75,469,841 (GRCm38) unclassified probably benign
R3923:Asic4 UTSW 1 75,451,227 (GRCm38) missense probably damaging 1.00
R4447:Asic4 UTSW 1 75,470,370 (GRCm38) unclassified probably benign
R5177:Asic4 UTSW 1 75,450,839 (GRCm38) missense probably damaging 1.00
R5208:Asic4 UTSW 1 75,451,226 (GRCm38) missense probably damaging 1.00
R5266:Asic4 UTSW 1 75,450,923 (GRCm38) missense probably benign 0.03
R5436:Asic4 UTSW 1 75,451,319 (GRCm38) missense probably benign 0.09
R5921:Asic4 UTSW 1 75,451,373 (GRCm38) missense probably benign 0.30
R6512:Asic4 UTSW 1 75,473,043 (GRCm38) nonsense probably null
R6530:Asic4 UTSW 1 75,472,335 (GRCm38) missense probably damaging 0.98
R7545:Asic4 UTSW 1 75,472,416 (GRCm38) missense probably damaging 0.98
R9129:Asic4 UTSW 1 75,469,825 (GRCm38) missense possibly damaging 0.77
R9322:Asic4 UTSW 1 75,469,818 (GRCm38) missense probably benign 0.38
Z1177:Asic4 UTSW 1 75,469,220 (GRCm38) missense probably benign 0.02
Predicted Primers PCR Primer

Posted On 2017-07-14