Incidental Mutation 'R6086:Tmem40'
ID482477
Institutional Source Beutler Lab
Gene Symbol Tmem40
Ensembl Gene ENSMUSG00000059900
Gene Nametransmembrane protein 40
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.053) question?
Stock #R6086 (G1)
Quality Score225.009
Status Validated
Chromosome6
Chromosomal Location115729131-115762410 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to T at 115733667 bp
ZygosityHeterozygous
Amino Acid Change Asparagine to Lysine at position 120 (N120K)
Ref Sequence ENSEMBL: ENSMUSP00000130407 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000072933] [ENSMUST00000112946] [ENSMUST00000166254] [ENSMUST00000170625]
Predicted Effect possibly damaging
Transcript: ENSMUST00000072933
AA Change: N145K

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000072704
Gene: ENSMUSG00000059900
AA Change: N145K

DomainStartEndE-ValueType
low complexity region 4 15 N/A INTRINSIC
low complexity region 45 71 N/A INTRINSIC
low complexity region 75 88 N/A INTRINSIC
low complexity region 120 139 N/A INTRINSIC
transmembrane domain 151 173 N/A INTRINSIC
transmembrane domain 177 199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000112946
AA Change: N186K

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000108568
Gene: ENSMUSG00000059900
AA Change: N186K

DomainStartEndE-ValueType
low complexity region 45 56 N/A INTRINSIC
low complexity region 86 112 N/A INTRINSIC
low complexity region 116 129 N/A INTRINSIC
Pfam:TMEM40 146 265 2.4e-74 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000127728
SMART Domains Protein: ENSMUSP00000116472
Gene: ENSMUSG00000059900

DomainStartEndE-ValueType
low complexity region 18 37 N/A INTRINSIC
transmembrane domain 49 71 N/A INTRINSIC
transmembrane domain 75 97 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000151849
Predicted Effect possibly damaging
Transcript: ENSMUST00000166254
AA Change: N145K

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000131697
Gene: ENSMUSG00000059900
AA Change: N145K

DomainStartEndE-ValueType
low complexity region 4 15 N/A INTRINSIC
low complexity region 45 71 N/A INTRINSIC
low complexity region 75 88 N/A INTRINSIC
low complexity region 120 139 N/A INTRINSIC
transmembrane domain 151 173 N/A INTRINSIC
transmembrane domain 177 199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000170625
AA Change: N120K

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000130407
Gene: ENSMUSG00000059900
AA Change: N120K

DomainStartEndE-ValueType
low complexity region 4 15 N/A INTRINSIC
low complexity region 95 114 N/A INTRINSIC
transmembrane domain 126 148 N/A INTRINSIC
transmembrane domain 152 174 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.2%
  • 20x: 94.9%
Validation Efficiency 96% (65/68)
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3110062M04Rik A G 6: 34,874,620 S127P probably damaging Het
Apob G T 12: 8,015,164 K4044N probably benign Het
Asic4 G A 1: 75,473,243 V468I possibly damaging Het
Atf4 T A 15: 80,257,453 V348D probably benign Het
Bcl9 A G 3: 97,205,524 V1205A possibly damaging Het
Bora A T 14: 99,062,294 Q234L possibly damaging Het
Cap2 C A 13: 46,635,712 P131Q probably damaging Het
Cdyl2 A G 8: 116,589,296 S318P probably damaging Het
Ces1a T G 8: 93,027,353 N341H probably benign Het
Crlf3 C T 11: 80,048,610 V352M possibly damaging Het
Cybb C G X: 9,450,750 D246H probably benign Het
Dnah2 T C 11: 69,516,008 T529A probably benign Het
Dnah9 C T 11: 65,989,915 D2619N probably damaging Het
Dnah9 A C 11: 66,085,174 S1350A probably benign Het
Dnajc6 C T 4: 101,597,807 S65L probably benign Het
Dnm3 C A 1: 162,321,033 R256S probably damaging Het
Enpp2 T C 15: 54,845,834 D795G probably damaging Het
Fah A T 7: 84,588,912 W367R probably damaging Het
Fam220a T A 5: 143,563,041 H69Q probably benign Het
Fgd3 T A 13: 49,287,296 T220S probably benign Het
Furin G T 7: 80,395,431 H248Q probably damaging Het
Gabrg1 A T 5: 70,754,053 L410Q probably damaging Het
Gm10801 AAGT AAGTAGT 2: 98,663,803 probably null Het
Gm9742 T C 13: 8,030,033 noncoding transcript Het
Gpcpd1 A G 2: 132,538,114 S252P probably damaging Het
Hnrnpdl C T 5: 100,036,481 G398S probably null Het
Hspa1l T C 17: 34,978,155 V390A possibly damaging Het
Htr3b T C 9: 48,947,298 S94G probably benign Het
Klf10 G T 15: 38,296,937 S271R probably benign Het
Klk1b3 T A 7: 44,201,734 L197Q probably damaging Het
Knl1 A G 2: 119,094,068 R1861G probably damaging Het
Lexm T C 4: 106,613,206 E218G probably damaging Het
Myo9a C T 9: 59,790,057 Q374* probably null Het
Myt1l G A 12: 29,832,332 G509R unknown Het
Ncapg T C 5: 45,693,236 L728P probably damaging Het
Nfxl1 A C 5: 72,541,019 F228V probably benign Het
Oc90 A G 15: 65,889,711 S153P probably damaging Het
Olfr1230 A G 2: 89,296,854 C139R probably damaging Het
Olfr1458 A G 19: 13,102,381 *308Q probably null Het
Pbk A T 14: 65,815,253 K182* probably null Het
Piezo1 A G 8: 122,501,657 F296S possibly damaging Het
Psmg1 T A 16: 95,980,044 Y288F probably damaging Het
Rab11fip4 G A 11: 79,683,480 D132N probably damaging Het
Recql4 T C 15: 76,704,587 D1051G probably damaging Het
Reep5 A C 18: 34,357,131 D104E probably damaging Het
Rnpepl1 T C 1: 92,917,681 Y441H probably damaging Het
Scnm1 T C 3: 95,130,285 I157V probably benign Het
Sema3b T C 9: 107,600,848 D446G probably damaging Het
Sema4d C T 13: 51,713,745 R190Q probably damaging Het
Slco3a1 G A 7: 74,318,590 R461C possibly damaging Het
Tcrg-C3 T C 13: 19,263,284 S136P probably damaging Het
Tenm2 T A 11: 36,008,646 I2562F possibly damaging Het
Thegl T A 5: 77,061,305 V458E probably benign Het
Tmod3 T C 9: 75,500,123 H351R probably benign Het
Tnn C T 1: 160,086,120 V1268M probably damaging Het
Tpmt T A 13: 47,035,030 D132V probably damaging Het
Trib1 G A 15: 59,654,475 R298H probably damaging Het
Tsen2 A G 6: 115,560,075 E264G probably benign Het
Urah A G 7: 140,836,798 I60M probably benign Het
Vmn2r26 T A 6: 124,039,560 S328T possibly damaging Het
Vmn2r27 G C 6: 124,191,999 T724R probably damaging Het
Washc2 A G 6: 116,256,216 probably null Het
Wdyhv1 C A 15: 58,150,628 A71E probably damaging Het
Zfp955b T A 17: 33,302,504 W316R probably benign Het
Zranb2 T C 3: 157,543,246 probably null Het
Other mutations in Tmem40
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0403:Tmem40 UTSW 6 115733985 splice site probably benign
R1740:Tmem40 UTSW 6 115738999 missense probably benign
R2238:Tmem40 UTSW 6 115731077 nonsense probably null
R2909:Tmem40 UTSW 6 115736381 critical splice donor site probably null
R3085:Tmem40 UTSW 6 115741615 missense possibly damaging 0.85
R5437:Tmem40 UTSW 6 115759031 intron probably benign
R5806:Tmem40 UTSW 6 115736412 missense probably benign 0.33
R7202:Tmem40 UTSW 6 115730459 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- AGACATCACTGCTTGTCCCC -3'
(R):5'- TCCGAAAGAAGTCTCCATCATGG -3'

Posted On2017-07-14