Mutagenetix > Incidental Mutation

Incidental Mutation 'R6079:Cdc20b'

482865

Institutional Source

Beutler Lab

Gene Symbol

Cdc20b

Ensembl Gene

ENSMUSG00000078926

Gene Name

cell division cycle 20B

Synonyms

EG622422, EG238896

Accession Numbers

Genbank: XM_138861

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R6079 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

113035111-113091195 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 113084042 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 485 (F485S)

Ref Sequence

ENSEMBL: ENSMUSP00000104867 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109244] [ENSMUST00000181117] [ENSMUST00000181568]

AlphaFold

D3Z3I0

Predicted Effect

probably damaging
Transcript: ENSMUST00000109244
AA Change: F485S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000104867
Gene: ENSMUSG00000078926
AA Change: F485S

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	517	2.01e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181117

SMART Domains

Protein: ENSMUSP00000137915
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	513	1.78e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000181568
AA Change: F443S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000137849
Gene: ENSMUSG00000078926
AA Change: F443S

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	431	475	2.01e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000232584

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,794,154	C172*	probably null	Het
Abcc8	T	C	7: 46,105,844	M1511V	probably benign	Het
Adamts2	T	A	11: 50,756,706	I302N	probably damaging	Het
Adgra2	G	A	8: 27,114,429	A511T	probably damaging	Het
Anxa10	C	A	8: 62,077,926	M83I	probably benign	Het
Atp2b4	TTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	TTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	1: 133,701,702		probably benign	Het
Edc4	C	T	8: 105,887,548	R19C	probably benign	Het
Fam90a1b	T	C	X: 94,356,585	N213S	probably benign	Het
Gkn3	C	T	6: 87,383,525	A163T	probably damaging	Het
Itgae	A	G	11: 73,115,574	E356G	possibly damaging	Het
Lgr6	C	T	1: 134,994,010	A199T	probably damaging	Het
Lrrc19	A	T	4: 94,643,343	F11I	probably benign	Het
Msl1	G	T	11: 98,798,693	G9C	probably benign	Het
Muc4	T	A	16: 32,755,247		probably benign	Het
Myo7a	C	T	7: 98,065,790	W1558*	probably null	Het
Olfr510	T	A	7: 108,668,205	V263E	probably damaging	Het
Pgk1	C	A	X: 106,194,492	L85I	possibly damaging	Het
Ppp4r1	G	A	17: 65,814,348	V268I	possibly damaging	Het
Rad23b	C	T	4: 55,370,400	A142V	probably damaging	Het
Satl1	T	C	X: 112,405,916	T281A	probably benign	Het
Shroom3	G	T	5: 92,943,086	V1151F	probably damaging	Het
Synrg	A	G	11: 84,024,300	E1044G	probably damaging	Het
Syt12	T	C	19: 4,456,868	D83G	probably benign	Het
Taf4b	T	C	18: 14,822,198	C603R	possibly damaging	Het
Tbx5	T	C	5: 119,883,146	S406P	probably benign	Het
Tfe3	T	C	X: 7,771,049	L231P	probably damaging	Het
Tns2	C	T	15: 102,108,934	R281C	probably damaging	Het
Vmn2r79	T	A	7: 87,004,111	V528D	possibly damaging	Het

Other mutations in Cdc20b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01625:Cdc20b	APN	13	113059785	missense	possibly damaging	0.74
IGL02603:Cdc20b	APN	13	113078755	missense	possibly damaging	0.88
IGL02663:Cdc20b	APN	13	113056131	critical splice donor site	probably null
IGL03024:Cdc20b	APN	13	113091042	missense	possibly damaging	0.58
IGL03379:Cdc20b	APN	13	113081202	missense	probably damaging	1.00
H8930:Cdc20b	UTSW	13	113083966	missense	probably damaging	1.00
R0207:Cdc20b	UTSW	13	113078612	missense	probably damaging	1.00
R0347:Cdc20b	UTSW	13	113059827	missense	probably damaging	0.97
R0448:Cdc20b	UTSW	13	113078657	missense	probably damaging	1.00
R0499:Cdc20b	UTSW	13	113055950	missense	probably benign	0.00
R1573:Cdc20b	UTSW	13	113055944	missense	probably benign	0.26
R1651:Cdc20b	UTSW	13	113078724	nonsense	probably null
R1786:Cdc20b	UTSW	13	113081134	missense	probably damaging	1.00
R1929:Cdc20b	UTSW	13	113071917	missense	probably benign	0.07
R2118:Cdc20b	UTSW	13	113078698	missense	probably benign	0.30
R3436:Cdc20b	UTSW	13	113078699	missense	probably damaging	0.99
R3508:Cdc20b	UTSW	13	113081042	missense	possibly damaging	0.80
R3837:Cdc20b	UTSW	13	113084008	missense	probably damaging	1.00
R4050:Cdc20b	UTSW	13	113064285	missense	probably benign
R4521:Cdc20b	UTSW	13	113081191	missense	probably damaging	1.00
R4786:Cdc20b	UTSW	13	113078734	missense	probably damaging	1.00
R6610:Cdc20b	UTSW	13	113064262	missense	probably benign	0.02
R6814:Cdc20b	UTSW	13	113083975	missense	probably damaging	1.00
R6872:Cdc20b	UTSW	13	113083975	missense	probably damaging	1.00
R6887:Cdc20b	UTSW	13	113078653	missense	possibly damaging	0.88
R7144:Cdc20b	UTSW	13	113083371	missense	probably benign	0.36
R7579:Cdc20b	UTSW	13	113037048	splice site	probably null
R7770:Cdc20b	UTSW	13	113078659	missense	probably benign	0.01
R8669:Cdc20b	UTSW	13	113071926	missense	possibly damaging	0.83
R8985:Cdc20b	UTSW	13	113059796	nonsense	probably null
R9182:Cdc20b	UTSW	13	113071969	critical splice donor site	probably null
R9309:Cdc20b	UTSW	13	113079938	missense	probably damaging	1.00
R9378:Cdc20b	UTSW	13	113056097	missense	probably benign	0.03
U15987:Cdc20b	UTSW	13	113084042	missense	probably damaging	1.00
X0064:Cdc20b	UTSW	13	113059742	missense	possibly damaging	0.59

Predicted Primers

PCR Primer
(F):5'- GCACATTCGCCATGCATCAG -3'
(R):5'- GATTGCTGCTGCCTAAAGC -3'

Sequencing Primer
(F):5'- TCGCCATGCATCAGAAGTTG -3'
(R):5'- GGAACCTGGACACTTTTAAGAGTTAG -3'

Posted On

2017-07-14