Incidental Mutation 'R6057:Zfp936'
ID482966
Institutional Source Beutler Lab
Gene Symbol Zfp936
Ensembl Gene ENSMUSG00000064194
Gene Namezinc finger protein 936
SynonymsEG435970, Gm9272, I1C0022H11Rik
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.623) question?
Stock #R6057 (G1)
Quality Score161.009
Status Not validated
Chromosome7
Chromosomal Location43114229-43192109 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 43190363 bp
ZygosityHeterozygous
Amino Acid Change Valine to Alanine at position 418 (V418A)
Ref Sequence ENSEMBL: ENSMUSP00000143800 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000072829] [ENSMUST00000200973] [ENSMUST00000202535] [ENSMUST00000205912]
Predicted Effect probably benign
Transcript: ENSMUST00000072829
AA Change: V417A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000072608
Gene: ENSMUSG00000064194
AA Change: V417A

DomainStartEndE-ValueType
KRAB 3 65 5.32e-19 SMART
ZnF_C2H2 148 170 5.9e-3 SMART
ZnF_C2H2 176 198 5.9e-3 SMART
ZnF_C2H2 204 226 1.4e-4 SMART
ZnF_C2H2 232 254 1.2e-3 SMART
ZnF_C2H2 260 282 7.37e-4 SMART
ZnF_C2H2 288 310 2.27e-4 SMART
ZnF_C2H2 316 338 2.09e-3 SMART
ZnF_C2H2 344 366 1.45e-2 SMART
ZnF_C2H2 372 394 1.82e-3 SMART
ZnF_C2H2 400 422 2.53e-2 SMART
ZnF_C2H2 428 450 4.54e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000200973
SMART Domains Protein: ENSMUSP00000144191
Gene: ENSMUSG00000064194

DomainStartEndE-ValueType
KRAB 4 66 1.4e-21 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000202535
AA Change: V418A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000143800
Gene: ENSMUSG00000064194
AA Change: V418A

DomainStartEndE-ValueType
KRAB 4 66 2.3e-21 SMART
ZnF_C2H2 149 171 2.6e-5 SMART
ZnF_C2H2 177 199 2.5e-5 SMART
ZnF_C2H2 205 227 5.9e-7 SMART
ZnF_C2H2 233 255 5.1e-6 SMART
ZnF_C2H2 261 283 3.1e-6 SMART
ZnF_C2H2 289 311 9.4e-7 SMART
ZnF_C2H2 317 339 8.7e-6 SMART
ZnF_C2H2 345 367 6.3e-5 SMART
ZnF_C2H2 373 395 7.7e-6 SMART
ZnF_C2H2 401 423 1.1e-4 SMART
ZnF_C2H2 429 451 2e-6 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000205912
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 97.9%
  • 20x: 93.6%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 87 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
5730596B20Rik A G 6: 52,179,520 probably benign Het
Acan A G 7: 79,099,782 S11G probably null Het
Ankrd13d A C 19: 4,282,228 V56G probably damaging Het
Arl15 A G 13: 113,967,615 Y76C probably damaging Het
Aspg G A 12: 112,120,998 C296Y probably damaging Het
Astl T A 2: 127,345,969 D101E probably benign Het
Bmpr2 A G 1: 59,842,818 N202S probably benign Het
Borcs7 T C 19: 46,701,564 *106Q probably null Het
Brip1 A G 11: 86,065,039 S883P possibly damaging Het
Cacng2 A T 15: 78,118,791 L34Q probably damaging Het
Catip A C 1: 74,362,918 D84A probably damaging Het
Ccdc162 A G 10: 41,634,041 L856S possibly damaging Het
Ccdc38 A G 10: 93,581,746 K500E probably damaging Het
Ccser2 T C 14: 36,941,165 K21E probably damaging Het
Cd93 A T 2: 148,441,519 Y636N probably damaging Het
Cep128 A T 12: 91,296,224 N300K possibly damaging Het
Cfap44 A G 16: 44,449,097 T1155A probably benign Het
Clec16a T C 16: 10,630,087 L550P probably damaging Het
Csmd3 T C 15: 47,755,391 Y1867C probably damaging Het
Cul3 A T 1: 80,271,532 I674N probably damaging Het
Cybb C G X: 9,450,750 D246H probably benign Het
Cyp2d34 T C 15: 82,616,351 H429R probably benign Het
Dab2ip T A 2: 35,692,255 C4* probably null Het
Dcaf1 A G 9: 106,854,247 E641G probably damaging Het
Dda1 T A 8: 71,474,632 probably benign Het
Dmgdh C T 13: 93,752,452 T866I probably benign Het
Ect2l T C 10: 18,161,502 T383A probably benign Het
Ets2 A G 16: 95,714,372 N181D probably benign Het
Ezh2 A G 6: 47,552,423 F222L probably damaging Het
Frem3 T C 8: 80,615,587 L1503P probably damaging Het
Fsip2 C A 2: 82,979,433 A2032E probably damaging Het
Gm1818 C T 12: 48,555,563 noncoding transcript Het
Gm9493 A G 19: 23,619,742 S1G probably damaging Het
Grin2b A G 6: 135,733,944 I868T possibly damaging Het
Ift22 A T 5: 136,911,133 T17S possibly damaging Het
Il4ra T C 7: 125,571,563 W216R probably damaging Het
Kcnj6 A T 16: 94,832,377 W274R probably damaging Het
Kctd19 G A 8: 105,396,450 H111Y probably damaging Het
Kremen2 A G 17: 23,742,705 V276A probably benign Het
Lig1 C A 7: 13,288,672 Q143K probably damaging Het
Lrp1 A T 10: 127,567,490 D2071E probably damaging Het
Macf1 C T 4: 123,510,743 M475I probably damaging Het
Mbtd1 G A 11: 93,929,659 A427T probably damaging Het
Myof A G 19: 37,926,981 probably null Het
Nbeal2 T C 9: 110,641,877 D308G possibly damaging Het
Ncdn T C 4: 126,745,031 Q665R probably benign Het
Nkd1 T C 8: 88,589,814 probably null Het
Nktr G A 9: 121,748,389 probably benign Het
Npc1l1 A T 11: 6,217,806 M995K possibly damaging Het
Olfr1129 C A 2: 87,576,019 R312S probably benign Het
Olfr300-ps1 T A 7: 86,443,189 C69* probably null Het
Olfr740 A T 14: 50,453,744 R231* probably null Het
Padi4 T C 4: 140,760,040 T184A probably damaging Het
Pgm2l1 C T 7: 100,266,674 P409S probably benign Het
Pgpep1 G A 8: 70,652,451 T53M probably damaging Het
Pgr T C 9: 8,902,005 L513P probably damaging Het
Pikfyve T A 1: 65,272,571 I1989N probably damaging Het
Prrc2a T C 17: 35,152,740 T1806A probably benign Het
Psd T C 19: 46,323,314 E309G possibly damaging Het
Qtrt1 C T 9: 21,412,003 T50I probably damaging Het
Rims2 A C 15: 39,675,020 T1320P probably damaging Het
Scn11a T G 9: 119,765,448 N1293T probably damaging Het
Scn8a T C 15: 100,974,667 F529S possibly damaging Het
Sec14l4 A T 11: 4,035,142 D25V possibly damaging Het
Sema3a C T 5: 13,565,865 R419C probably damaging Het
Slc12a1 T C 2: 125,190,213 Y595H probably damaging Het
Slc25a28 A T 19: 43,666,925 H170Q possibly damaging Het
Slc26a1 T A 5: 108,673,765 Q86L probably damaging Het
Slc48a1 T A 15: 97,789,917 W51R probably damaging Het
Sptlc3 T A 2: 139,581,613 V309D probably damaging Het
Srcap G A 7: 127,541,356 S1375N probably damaging Het
Tanc1 C A 2: 59,817,493 H986Q possibly damaging Het
Tbc1d4 A G 14: 101,489,917 V486A probably damaging Het
Tceanc2 T C 4: 107,147,579 D124G probably damaging Het
Tdrd9 A G 12: 112,013,286 M402V possibly damaging Het
Tmem132d T C 5: 127,784,870 D729G probably damaging Het
Tmem62 T A 2: 120,977,462 I55N probably damaging Het
Tnfrsf21 A T 17: 43,039,715 N257Y possibly damaging Het
Trappc3 T C 4: 126,274,041 L131P probably damaging Het
Vmn2r25 A T 6: 123,822,941 M814K possibly damaging Het
Vmn2r87 T C 10: 130,472,357 I671V probably benign Het
Vwa8 A T 14: 79,082,873 D1108V probably benign Het
Xrcc4 C G 13: 89,991,079 A241P possibly damaging Het
Xylt1 T A 7: 117,591,908 D310E probably benign Het
Zfp27 T G 7: 29,895,019 H507P possibly damaging Het
Zfp341 C T 2: 154,625,034 P108S probably benign Het
Zfp641 T C 15: 98,292,935 N76S probably benign Het
Other mutations in Zfp936
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02231:Zfp936 APN 7 43187485 splice site probably null
IGL02245:Zfp936 APN 7 43187298 critical splice donor site probably null
IGL02335:Zfp936 APN 7 43187267 missense probably damaging 1.00
FR4340:Zfp936 UTSW 7 43189489 missense possibly damaging 0.63
R0437:Zfp936 UTSW 7 43189310 missense probably benign 0.00
R3899:Zfp936 UTSW 7 43189734 missense possibly damaging 0.93
R4120:Zfp936 UTSW 7 43190206 missense probably benign 0.10
R4406:Zfp936 UTSW 7 43190324 missense possibly damaging 0.82
R4959:Zfp936 UTSW 7 43189610 missense probably damaging 1.00
R5023:Zfp936 UTSW 7 43187257 missense probably damaging 1.00
R5163:Zfp936 UTSW 7 43190240 missense probably damaging 1.00
R5182:Zfp936 UTSW 7 43189907 missense probably damaging 1.00
R5292:Zfp936 UTSW 7 43189335 nonsense probably null
R5668:Zfp936 UTSW 7 43190434 missense possibly damaging 0.93
R6901:Zfp936 UTSW 7 43190043 missense probably damaging 1.00
R7139:Zfp936 UTSW 7 43190291 missense possibly damaging 0.54
R7258:Zfp936 UTSW 7 43190379 missense probably damaging 1.00
R7440:Zfp936 UTSW 7 43187261 missense probably damaging 1.00
R7537:Zfp936 UTSW 7 43189815 nonsense probably null
R7561:Zfp936 UTSW 7 43189915 missense probably damaging 0.98
R7662:Zfp936 UTSW 7 43189912 nonsense probably null
R7775:Zfp936 UTSW 7 43190296 missense possibly damaging 0.70
R7778:Zfp936 UTSW 7 43190296 missense possibly damaging 0.70
R8016:Zfp936 UTSW 7 43189424 missense not run
Predicted Primers PCR Primer
(F):5'- CTGTCTTCGGGTACATGAAAGAACAC -3'
(R):5'- TGCACTTTGAGATTGCCTTGTG -3'

Posted On2017-07-14