Mutagenetix > Incidental Mutation

Incidental Mutation 'R6048:Dact2'

483417

Institutional Source

Beutler Lab

Gene Symbol

Dact2

Ensembl Gene

ENSMUSG00000048826

Gene Name

dishevelled-binding antagonist of beta-catenin 2

Synonyms

dapper2, Dpr2, Frd2, 2900084M21Rik

MMRRC Submission

044216-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6048 (G1)

Quality Score

161.009

Status

Validated

Chromosome

Chromosomal Location

14195231-14203831 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 14197305 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 211 (L211P)

Ref Sequence

ENSEMBL: ENSMUSP00000051638 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053218]

AlphaFold

Q7TN08

Predicted Effect

probably damaging
Transcript: ENSMUST00000053218
AA Change: L211P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000051638
Gene: ENSMUSG00000048826
AA Change: L211P

Domain	Start	End	E-Value	Type
Pfam:Dapper	19	757	1.8e-231	PFAM

Meta Mutation Damage Score

0.4130

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.5%

Validation Efficiency

98% (60/61)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele develop normally but display enhanced keratinocyte migration and accelerated re-epithelialization during cutaneous wound healing. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010111I01Rik	T	A	13: 63,240,325	F39L	probably damaging	Het
Aldh1a2	T	C	9: 71,261,767	I177T	probably damaging	Het
Arl4c	A	G	1: 88,701,628	S13P	possibly damaging	Het
Bst1	G	T	5: 43,818,964		probably benign	Het
Carmil3	G	A	14: 55,503,845	R1029Q	probably benign	Het
Cflar	A	G	1: 58,741,043	T269A	probably benign	Het
Clcnka	T	A	4: 141,394,487	Y236F	probably damaging	Het
Clmp	T	A	9: 40,771,109	I63N	probably damaging	Het
Cln6	T	C	9: 62,844,626	L44P	probably damaging	Het
Col5a3	C	T	9: 20,807,619	A280T	unknown	Het
Dclk2	G	T	3: 86,905,965	Q225K	probably damaging	Het
Ddx60	T	C	8: 62,000,582	F1245S	probably benign	Het
Eif3i	T	C	4: 129,593,352	N240S	probably benign	Het
Enpp1	T	C	10: 24,660,254	Y416C	probably damaging	Het
Epb42	T	A	2: 121,024,408	R565S	probably benign	Het
Eral1	G	A	11: 78,075,783	P217L	probably benign	Het
Fbxl16	A	G	17: 25,816,993	Y188C	probably benign	Het
Fgd3	G	A	13: 49,273,748	P503S	probably benign	Het
Frem3	T	C	8: 80,613,433	L785P	probably benign	Het
Gadl1	A	G	9: 116,006,701		probably null	Het
Gm10787	C	T	10: 77,021,842		noncoding transcript	Het
Gm11639	A	G	11: 104,944,433	K3540E	unknown	Het
Gm17359	T	C	3: 79,405,885	S89P	probably damaging	Het
Gm6563	A	G	19: 23,675,882	K12R	probably benign	Het
Grm5	T	C	7: 88,026,550	L424P	probably damaging	Het
Hist1h2ag	A	G	13: 22,042,836	V31A	probably benign	Het
Il17re	T	C	6: 113,470,108	S607P	possibly damaging	Het
Ints7	A	G	1: 191,621,412		probably benign	Het
Itih1	A	G	14: 30,929,823	M854T	possibly damaging	Het
Kif1b	A	G	4: 149,263,629	L315P	probably damaging	Het
Ms4a6b	T	C	19: 11,520,370	V11A	possibly damaging	Het
Mxd1	C	A	6: 86,650,984	D191Y	probably damaging	Het
Nol8	T	A	13: 49,653,684		probably null	Het
Ntn4	T	A	10: 93,707,266		probably null	Het
Nxph1	A	T	6: 9,247,103	T25S	probably benign	Het
Olfr1344	A	C	7: 6,440,355	T152P	possibly damaging	Het
Olfr202	C	T	16: 59,283,979	V173I	probably benign	Het
Olfr351	A	G	2: 36,859,841	V169A	probably benign	Het
Olfr410	A	T	11: 74,335,135	I32K	probably benign	Het
Olfr583	A	G	7: 103,051,319	N7S	probably benign	Het
Olfr818	A	G	10: 129,945,826	F79L	possibly damaging	Het
Oxsm	T	A	14: 16,242,308	M154L	possibly damaging	Het
Papolg	A	C	11: 23,891,815	I36S	probably benign	Het
Pde3b	T	A	7: 114,508,267	H544Q	probably benign	Het
Pdzd2	A	T	15: 12,592,570		probably null	Het
Prkce	C	A	17: 86,493,347	P397Q	probably benign	Het
Ralgapa2	T	C	2: 146,434,845	S492G	possibly damaging	Het
Rnf216	A	G	5: 143,068,904	Y630H	probably damaging	Het
Ruvbl1	C	T	6: 88,482,991	T211I	possibly damaging	Het
Scyl2	A	T	10: 89,645,486	D666E	probably benign	Het
Sertad2	A	G	11: 20,648,436	T211A	probably benign	Het
Sipa1l1	A	T	12: 82,440,869	Q1639L	probably benign	Het
Snx5	T	C	2: 144,259,153	D98G	probably damaging	Het
Sorcs2	G	T	5: 36,027,988		probably null	Het
Tanc2	G	A	11: 105,867,717	R768Q	probably damaging	Het
Tmem132d	A	G	5: 128,269,117	S114P	probably benign	Het
Tns2	G	A	15: 102,111,411	G579R	probably damaging	Het
Usp37	A	T	1: 74,478,136		probably null	Het
Vmn1r79	T	C	7: 12,176,521	I110T	probably damaging	Het
Vps37a	C	T	8: 40,528,322	L69F	probably damaging	Het
Xirp2	T	A	2: 67,508,243	I276N	possibly damaging	Het
Zfp946	G	A	17: 22,454,840	E192K	probably benign	Het

Other mutations in Dact2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01867:Dact2	APN	17	14195670	missense	probably damaging	0.98
R0597:Dact2	UTSW	17	14197041	missense	probably benign
R1657:Dact2	UTSW	17	14197990	missense	probably benign	0.03
R1717:Dact2	UTSW	17	14197913	missense	probably benign	0.11
R1734:Dact2	UTSW	17	14196639	missense	probably benign	0.00
R1883:Dact2	UTSW	17	14197823	missense	possibly damaging	0.81
R2238:Dact2	UTSW	17	14197050	missense	probably damaging	0.99
R2878:Dact2	UTSW	17	14195914	missense	probably damaging	1.00
R4290:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4291:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4902:Dact2	UTSW	17	14196729	missense	possibly damaging	0.91
R5029:Dact2	UTSW	17	14195852	missense	probably benign	0.00
R5084:Dact2	UTSW	17	14197952	missense	possibly damaging	0.94
R5436:Dact2	UTSW	17	14195748	missense	probably damaging	1.00
R5837:Dact2	UTSW	17	14196253	missense	probably damaging	1.00
R5847:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R6377:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R7013:Dact2	UTSW	17	14203534	missense	probably benign
R7268:Dact2	UTSW	17	14196535	missense	probably benign	0.01
R7425:Dact2	UTSW	17	14196331	missense	probably damaging	1.00
R8725:Dact2	UTSW	17	14196884	nonsense	probably null
R8727:Dact2	UTSW	17	14196884	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CTTCTTTGGGCAGAGCGAAG -3'
(R):5'- CAGGAGCAGTTAACTGTCCC -3'

Sequencing Primer
(F):5'- GACTCTGCAGGGCCACC -3'
(R):5'- AGTTAACTGTCCCAGGTAGCGTC -3'

Posted On

2017-07-14