Mutagenetix > Incidental Mutation

Incidental Mutation 'R6064:Fstl5'

483965

Institutional Source

Beutler Lab

Gene Symbol

Fstl5

Ensembl Gene

ENSMUSG00000034098

Gene Name

follistatin-like 5

Synonyms

9130207J01Rik

MMRRC Submission

044228-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R6064 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

75981582-76617317 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 76229605 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 135 (F135L)

Ref Sequence

ENSEMBL: ENSMUSP00000125393 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038364] [ENSMUST00000160261]

AlphaFold

Q8BFR2

Predicted Effect

probably benign
Transcript: ENSMUST00000038364
AA Change: F135L

PolyPhen 2 Score 0.134 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000038506
Gene: ENSMUSG00000034098
AA Change: F135L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
KAZAL	88	133	2.16e-9	SMART
IGc2	261	328	1.11e-5	SMART
IGc2	353	420	3.85e-14	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159686

Predicted Effect

probably benign
Transcript: ENSMUST00000160261
AA Change: F135L

PolyPhen 2 Score 0.134 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000125393
Gene: ENSMUSG00000034098
AA Change: F135L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
KAZAL	88	133	2.16e-9	SMART
IGc2	261	328	1.11e-5	SMART
IGc2	353	420	3.85e-14	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgra3	C	T	5: 50,117,667 (GRCm39)	D1294N	probably damaging	Het
Atf1	A	G	15: 100,150,029 (GRCm39)	T92A	probably benign	Het
Bach1	A	G	16: 87,526,752 (GRCm39)	D738G	probably damaging	Het
Catsper3	T	C	13: 55,954,065 (GRCm39)	F278L	probably damaging	Het
Chsy3	GT	G	18: 59,309,238 (GRCm39)	163	probably null	Het
Cmya5	T	A	13: 93,226,157 (GRCm39)	N2977I	probably damaging	Het
Cnbd1	T	A	4: 18,895,084 (GRCm39)	E219D	probably benign	Het
Dst	A	G	1: 34,233,132 (GRCm39)	D3556G	probably damaging	Het
Duox1	A	G	2: 122,151,243 (GRCm39)	E306G	probably benign	Het
Fyb1	A	C	15: 6,668,349 (GRCm39)	K514T	probably damaging	Het
Gsap	T	A	5: 21,434,223 (GRCm39)	C280S	possibly damaging	Het
Lrig1	T	C	6: 94,603,428 (GRCm39)	E240G	probably damaging	Het
Macc1	A	T	12: 119,409,400 (GRCm39)	H56L	probably benign	Het
Mroh2a	GCCC	GC	1: 88,159,979 (GRCm39)		probably null	Het
Muc6	T	C	7: 141,234,640 (GRCm39)	R696G	probably damaging	Het
Nup210	T	C	6: 91,032,273 (GRCm39)	I32V	probably benign	Het
Or52n2c	C	T	7: 104,574,599 (GRCm39)	R124H	probably benign	Het
Or5d16	A	G	2: 87,773,828 (GRCm39)	M48T	probably benign	Het
Or5h17	T	G	16: 58,820,186 (GRCm39)	I46S	probably damaging	Het
Ovch2	T	C	7: 107,395,779 (GRCm39)	T80A	probably damaging	Het
Rad51ap2	T	A	12: 11,507,418 (GRCm39)	Y447N	possibly damaging	Het
Rb1cc1	A	G	1: 6,319,958 (GRCm39)	T1126A	probably benign	Het
Scgb1b10	T	C	7: 31,800,627 (GRCm39)	L72S	probably damaging	Het
Slc25a33	A	G	4: 149,836,921 (GRCm39)	V141A	probably benign	Het
Tbx21	T	C	11: 97,005,737 (GRCm39)	Y76C	probably damaging	Het
Tmem235	T	C	11: 117,753,764 (GRCm39)	V107A	possibly damaging	Het
Vnn1	G	T	10: 23,770,807 (GRCm39)	A12S	probably benign	Het
Zfp606	T	G	7: 12,214,960 (GRCm39)	W63G	possibly damaging	Het
Zfp788	T	A	7: 41,297,878 (GRCm39)	F119L	probably benign	Het

Other mutations in Fstl5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01632:Fstl5	APN	3	76,615,135 (GRCm39)	missense	probably benign	0.30
IGL01658:Fstl5	APN	3	76,389,562 (GRCm39)	missense	possibly damaging	0.70
IGL01917:Fstl5	APN	3	76,615,153 (GRCm39)	missense	probably damaging	1.00
IGL02073:Fstl5	APN	3	76,566,959 (GRCm39)	splice site	probably benign
IGL02329:Fstl5	APN	3	76,496,302 (GRCm39)	missense	probably damaging	1.00
IGL02651:Fstl5	APN	3	76,500,841 (GRCm39)	missense	probably damaging	1.00
IGL02967:Fstl5	APN	3	76,229,498 (GRCm39)	missense	probably damaging	1.00
IGL03004:Fstl5	APN	3	76,555,738 (GRCm39)	splice site	probably benign
IGL03107:Fstl5	APN	3	76,443,618 (GRCm39)	missense	probably damaging	1.00
IGL03113:Fstl5	APN	3	76,337,099 (GRCm39)	nonsense	probably null
P0038:Fstl5	UTSW	3	76,052,369 (GRCm39)	missense	probably damaging	1.00
PIT4131001:Fstl5	UTSW	3	76,567,006 (GRCm39)	missense	probably damaging	0.99
R0015:Fstl5	UTSW	3	76,229,498 (GRCm39)	missense	probably damaging	1.00
R0015:Fstl5	UTSW	3	76,229,498 (GRCm39)	missense	probably damaging	1.00
R0032:Fstl5	UTSW	3	76,555,742 (GRCm39)	splice site	probably benign
R0032:Fstl5	UTSW	3	76,555,742 (GRCm39)	splice site	probably benign
R0078:Fstl5	UTSW	3	76,566,952 (GRCm39)	splice site	probably benign
R0137:Fstl5	UTSW	3	76,614,786 (GRCm39)	missense	probably damaging	1.00
R0183:Fstl5	UTSW	3	76,229,579 (GRCm39)	missense	possibly damaging	0.86
R0330:Fstl5	UTSW	3	76,615,060 (GRCm39)	missense	possibly damaging	0.80
R0427:Fstl5	UTSW	3	76,615,034 (GRCm39)	nonsense	probably null
R0687:Fstl5	UTSW	3	76,615,119 (GRCm39)	missense	possibly damaging	0.62
R1642:Fstl5	UTSW	3	76,317,929 (GRCm39)	missense	possibly damaging	0.80
R1765:Fstl5	UTSW	3	76,500,783 (GRCm39)	missense	possibly damaging	0.90
R1900:Fstl5	UTSW	3	76,615,467 (GRCm39)	missense	probably damaging	1.00
R1996:Fstl5	UTSW	3	76,615,141 (GRCm39)	missense	probably benign	0.19
R2157:Fstl5	UTSW	3	76,615,372 (GRCm39)	missense	possibly damaging	0.46
R2228:Fstl5	UTSW	3	76,389,659 (GRCm39)	missense	probably damaging	1.00
R2851:Fstl5	UTSW	3	76,337,045 (GRCm39)	splice site	probably benign
R4021:Fstl5	UTSW	3	76,536,282 (GRCm39)	missense	probably benign	0.00
R4086:Fstl5	UTSW	3	76,555,593 (GRCm39)	missense	probably damaging	1.00
R4777:Fstl5	UTSW	3	76,500,807 (GRCm39)	missense	probably damaging	1.00
R4829:Fstl5	UTSW	3	76,229,489 (GRCm39)	missense	probably damaging	1.00
R4934:Fstl5	UTSW	3	76,496,272 (GRCm39)	missense	probably damaging	1.00
R4955:Fstl5	UTSW	3	76,131,183 (GRCm39)	critical splice donor site	probably null
R4977:Fstl5	UTSW	3	76,317,801 (GRCm39)	nonsense	probably null
R5166:Fstl5	UTSW	3	76,536,267 (GRCm39)	missense	possibly damaging	0.86
R5232:Fstl5	UTSW	3	76,052,284 (GRCm39)	missense	possibly damaging	0.89
R5313:Fstl5	UTSW	3	76,500,812 (GRCm39)	missense	possibly damaging	0.90
R5584:Fstl5	UTSW	3	76,229,574 (GRCm39)	missense	probably damaging	1.00
R5647:Fstl5	UTSW	3	76,496,399 (GRCm39)	missense	probably damaging	1.00
R5842:Fstl5	UTSW	3	76,229,590 (GRCm39)	missense	possibly damaging	0.94
R5978:Fstl5	UTSW	3	76,052,392 (GRCm39)	missense	probably damaging	1.00
R6007:Fstl5	UTSW	3	76,317,899 (GRCm39)	missense	probably damaging	1.00
R6327:Fstl5	UTSW	3	76,615,108 (GRCm39)	missense	probably benign	0.31
R6386:Fstl5	UTSW	3	76,229,373 (GRCm39)	missense	probably benign	0.13
R6523:Fstl5	UTSW	3	76,443,641 (GRCm39)	missense	probably benign	0.00
R6852:Fstl5	UTSW	3	76,615,162 (GRCm39)	missense	probably damaging	1.00
R6861:Fstl5	UTSW	3	76,229,523 (GRCm39)	missense	probably damaging	1.00
R6866:Fstl5	UTSW	3	76,229,532 (GRCm39)	missense	probably damaging	0.99
R7100:Fstl5	UTSW	3	76,443,600 (GRCm39)	missense	probably benign	0.11
R7341:Fstl5	UTSW	3	76,389,704 (GRCm39)	splice site	probably null
R7495:Fstl5	UTSW	3	76,615,099 (GRCm39)	missense	possibly damaging	0.85
R7558:Fstl5	UTSW	3	76,337,092 (GRCm39)	missense	possibly damaging	0.95
R7731:Fstl5	UTSW	3	76,569,069 (GRCm39)	missense	probably damaging	1.00
R7787:Fstl5	UTSW	3	76,337,131 (GRCm39)	missense	probably damaging	1.00
R7852:Fstl5	UTSW	3	76,615,275 (GRCm39)	missense	probably benign	0.00
R7874:Fstl5	UTSW	3	76,569,093 (GRCm39)	missense	probably benign	0.10
R7881:Fstl5	UTSW	3	76,443,605 (GRCm39)	missense	probably damaging	1.00
R7986:Fstl5	UTSW	3	76,337,097 (GRCm39)	missense	probably damaging	0.98
R8039:Fstl5	UTSW	3	76,555,725 (GRCm39)	missense	possibly damaging	0.69
R8050:Fstl5	UTSW	3	76,614,810 (GRCm39)	missense	probably benign	0.00
R8844:Fstl5	UTSW	3	76,337,154 (GRCm39)	missense	possibly damaging	0.71
R8929:Fstl5	UTSW	3	76,615,138 (GRCm39)	missense	probably damaging	0.98
R9012:Fstl5	UTSW	3	76,567,027 (GRCm39)	missense	probably damaging	1.00
R9069:Fstl5	UTSW	3	76,615,416 (GRCm39)	missense	probably damaging	0.99
R9221:Fstl5	UTSW	3	76,569,114 (GRCm39)	missense	probably damaging	0.98
R9373:Fstl5	UTSW	3	76,555,669 (GRCm39)	nonsense	probably null
R9427:Fstl5	UTSW	3	76,229,583 (GRCm39)	missense
R9490:Fstl5	UTSW	3	76,615,060 (GRCm39)	missense	possibly damaging	0.80
R9603:Fstl5	UTSW	3	76,496,260 (GRCm39)	missense	probably damaging	1.00
Z1176:Fstl5	UTSW	3	76,615,289 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGGAGGCACTGTGTTATCAAC -3'
(R):5'- GGCCAGCTTTAGTCTCATAAATG -3'

Sequencing Primer
(F):5'- TGTGTTATCAACAGAGAGACAAGGC -3'
(R):5'- CTTTTTTCAGATGCTTACAGAG -3'

Posted On

2017-07-14