Incidental Mutation 'R6064:Tmem235'
ID483980
Institutional Source Beutler Lab
Gene Symbol Tmem235
Ensembl Gene ENSMUSG00000070330
Gene Nametransmembrane protein 235
SynonymsGm12581, Tmem235
MMRRC Submission 044228-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.049) question?
Stock #R6064 (G1)
Quality Score225.009
Status Not validated
Chromosome11
Chromosomal Location117860752-117865543 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 117862938 bp
ZygosityHeterozygous
Amino Acid Change Valine to Alanine at position 107 (V107A)
Ref Sequence ENSEMBL: ENSMUSP00000091432 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000093905]
Predicted Effect possibly damaging
Transcript: ENSMUST00000093905
AA Change: V107A

PolyPhen 2 Score 0.840 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000091432
Gene: ENSMUSG00000070330
AA Change: V107A

DomainStartEndE-ValueType
Pfam:PMP22_Claudin 4 190 9.7e-14 PFAM
Pfam:Claudin_2 16 192 1.3e-45 PFAM
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.7%
  • 20x: 93.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 29 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgra3 C T 5: 49,960,325 D1294N probably damaging Het
Atf1 A G 15: 100,252,148 T92A probably benign Het
Bach1 A G 16: 87,729,864 D738G probably damaging Het
Catsper3 T C 13: 55,806,252 F278L probably damaging Het
Chsy3 GT G 18: 59,176,166 probably null Het
Cmya5 T A 13: 93,089,649 N2977I probably damaging Het
Cnbd1 T A 4: 18,895,084 E219D probably benign Het
Dst A G 1: 34,194,051 D3556G probably damaging Het
Duox1 A G 2: 122,320,762 E306G probably benign Het
Fstl5 T A 3: 76,322,298 F135L probably benign Het
Fyb A C 15: 6,638,868 K514T probably damaging Het
Gsap T A 5: 21,229,225 C280S possibly damaging Het
Lrig1 T C 6: 94,626,447 E240G probably damaging Het
Macc1 A T 12: 119,445,665 H56L probably benign Het
Mroh2a GCCC GC 1: 88,232,257 probably null Het
Muc6 T C 7: 141,648,374 R696G probably damaging Het
Nup210 T C 6: 91,055,291 I32V probably benign Het
Olfr1155 A G 2: 87,943,484 M48T probably benign Het
Olfr183 T G 16: 58,999,823 I46S probably damaging Het
Olfr668 C T 7: 104,925,392 R124H probably benign Het
Ovch2 T C 7: 107,796,572 T80A probably damaging Het
Rad51ap2 T A 12: 11,457,417 Y447N possibly damaging Het
Rb1cc1 A G 1: 6,249,734 T1126A probably benign Het
Scgb1b10 T C 7: 32,101,202 L72S probably damaging Het
Slc25a33 A G 4: 149,752,464 V141A probably benign Het
Tbx21 T C 11: 97,114,911 Y76C probably damaging Het
Vnn1 G T 10: 23,894,909 A12S probably benign Het
Zfp606 T G 7: 12,481,033 W63G possibly damaging Het
Zfp788 T A 7: 41,648,454 F119L probably benign Het
Other mutations in Tmem235
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01371:Tmem235 APN 11 117862306 missense possibly damaging 0.95
R0497:Tmem235 UTSW 11 117864351 missense probably benign 0.01
R0564:Tmem235 UTSW 11 117860848 missense possibly damaging 0.86
R3778:Tmem235 UTSW 11 117862300 missense probably benign 0.08
R5364:Tmem235 UTSW 11 117864194 nonsense probably null
R7139:Tmem235 UTSW 11 117860897 missense probably damaging 0.99
R7567:Tmem235 UTSW 11 117864141 missense probably benign 0.04
R7884:Tmem235 UTSW 11 117864207 missense probably benign 0.08
R7904:Tmem235 UTSW 11 117860891 missense probably damaging 1.00
X0063:Tmem235 UTSW 11 117862294 missense probably benign 0.08
Predicted Primers PCR Primer
(F):5'- GCGTCTGCTTGAATCTCTGC -3'
(R):5'- CCCAGTGATAAAGGCTGCTGAG -3'

Sequencing Primer
(F):5'- GAATCTCTGCTCTGGTGACC -3'
(R):5'- CTGAGGAATGACCCCTGTGTG -3'
Posted On2017-07-14