Mutagenetix > Incidental Mutation

Incidental Mutation 'R6052:Wnt3'

484178

Institutional Source

Beutler Lab

Gene Symbol

Wnt3

Ensembl Gene

ENSMUSG00000000125

Gene Name

wingless-type MMTV integration site family, member 3

Synonyms

Wnt-3, Int-4

MMRRC Submission

044220-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6052 (G1)

Quality Score

110.008

Status

Validated

Chromosome

Chromosomal Location

103664976-103708783 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 103699000 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 35 (Y35*)

Ref Sequence

ENSEMBL: ENSMUSP00000000127 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000127]

AlphaFold

P17553

Predicted Effect

probably null
Transcript: ENSMUST00000000127
AA Change: Y35*

SMART Domains

Protein: ENSMUSP00000000127
Gene: ENSMUSG00000000125
AA Change: Y35*

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
WNT1	47	355	1.24e-216	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.7%

Validation Efficiency

96% (80/83)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants develop to the egg cylinder stage, but fail to form a primitive streak, mesoderm, or node, and die by embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	G	17: 24,537,165 (GRCm39)	F473L	probably benign	Het
Ankrd17	T	C	5: 90,401,691 (GRCm39)	I1449V	probably benign	Het
Aox4	T	A	1: 58,293,477 (GRCm39)	L943*	probably null	Het
Arap1	G	A	7: 101,053,240 (GRCm39)	V1257M	probably damaging	Het
B3gnt9	T	C	8: 105,981,230 (GRCm39)	S53G	probably benign	Het
Baiap3	A	T	17: 25,467,444 (GRCm39)		probably benign	Het
Canx	T	C	11: 50,187,946 (GRCm39)	D554G	possibly damaging	Het
Cbfa2t2	T	C	2: 154,352,501 (GRCm39)	V165A	probably damaging	Het
Ccdc17	C	T	4: 116,457,145 (GRCm39)		probably null	Het
Cdcp1	A	G	9: 123,014,396 (GRCm39)	I126T	probably benign	Het
Cdk13	A	T	13: 17,895,800 (GRCm39)	D1036E	probably damaging	Het
Cfap157	A	G	2: 32,669,863 (GRCm39)	L240P	probably damaging	Het
Clec18a	C	A	8: 111,805,448 (GRCm39)	E218*	probably null	Het
Col3a1	T	C	1: 45,384,173 (GRCm39)		probably benign	Het
Dennd4a	A	G	9: 64,794,227 (GRCm39)	E682G	probably damaging	Het
Dnah14	T	C	1: 181,494,052 (GRCm39)	V1736A	possibly damaging	Het
Dpep2	T	A	8: 106,717,270 (GRCm39)	D162V	possibly damaging	Het
Drd3	C	A	16: 43,641,646 (GRCm39)	P321T	probably benign	Het
Egfr	A	G	11: 16,861,554 (GRCm39)	H1111R	probably benign	Het
Entpd1	T	C	19: 40,708,928 (GRCm39)	S58P	probably damaging	Het
Epha3	C	A	16: 63,423,967 (GRCm39)	V541L	possibly damaging	Het
Eri3	T	A	4: 117,421,825 (GRCm39)	D34E	probably damaging	Het
Eya1	T	C	1: 14,353,374 (GRCm39)	D58G	probably damaging	Het
Fat3	T	A	9: 15,833,975 (GRCm39)	S26C	probably null	Het
Fgl1	T	C	8: 41,653,548 (GRCm39)	D115G	probably damaging	Het
Fitm2	T	C	2: 163,312,036 (GRCm39)	Y59C	probably damaging	Het
Fras1	T	A	5: 96,912,725 (GRCm39)	I3343N	probably damaging	Het
Gba2	C	A	4: 43,568,330 (GRCm39)	C679F	probably damaging	Het
Glis2	G	A	16: 4,431,603 (GRCm39)		probably benign	Het
Gm5150	T	A	3: 16,044,917 (GRCm39)	I103F	probably damaging	Het
Hhipl2	T	C	1: 183,204,965 (GRCm39)	S313P	possibly damaging	Het
Hmgcs1	A	G	13: 120,166,995 (GRCm39)	D474G	probably benign	Het
Homer3	C	T	8: 70,744,076 (GRCm39)	Q267*	probably null	Het
Hsd17b8	A	G	17: 34,246,429 (GRCm39)	L118P	probably damaging	Het
Igkv8-28	C	T	6: 70,120,673 (GRCm39)	G90D	probably damaging	Het
Kalrn	T	A	16: 34,181,255 (GRCm39)	I128F	probably damaging	Het
Krt4	A	G	15: 101,831,194 (GRCm39)		probably null	Het
Lamb2	T	A	9: 108,364,811 (GRCm39)	C1188*	probably null	Het
Lsr	A	T	7: 30,658,042 (GRCm39)	M355K	probably damaging	Het
Map3k11	T	G	19: 5,747,430 (GRCm39)	D555E	probably benign	Het
Myocd	A	G	11: 65,087,082 (GRCm39)	Y282H	probably damaging	Het
Nae1	T	C	8: 105,261,176 (GRCm39)	I7M	probably benign	Het
Nprl3	G	T	11: 32,205,453 (GRCm39)	H102N	possibly damaging	Het
Nup62	T	A	7: 44,478,464 (GRCm39)	F160I	possibly damaging	Het
Or10w1	C	A	19: 13,631,871 (GRCm39)	P26Q	possibly damaging	Het
Or2d3c	A	T	7: 106,525,896 (GRCm39)	F257I	probably benign	Het
Or2r2	T	A	6: 42,463,588 (GRCm39)	T180S	possibly damaging	Het
Or5p76	A	G	7: 108,122,945 (GRCm39)	S71P	probably benign	Het
Or6c35	C	A	10: 129,169,071 (GRCm39)	T107K	possibly damaging	Het
Osbpl10	T	C	9: 114,896,383 (GRCm39)		probably null	Het
Pcdh9	C	T	14: 94,123,282 (GRCm39)	V963I	probably benign	Het
Phf12	A	G	11: 77,909,044 (GRCm39)	R375G	probably benign	Het
Piezo1	G	A	8: 123,233,008 (GRCm39)	T108M	probably damaging	Het
Plxnc1	T	A	10: 94,779,635 (GRCm39)	Q269L	probably benign	Het
Pomgnt1	T	A	4: 116,008,799 (GRCm39)	N11K	possibly damaging	Het
Pramel22	T	A	4: 143,382,222 (GRCm39)	D158V	probably damaging	Het
Prkd1	A	T	12: 50,413,083 (GRCm39)		probably null	Het
Prpf40a	T	C	2: 53,049,293 (GRCm39)	T190A	probably benign	Het
Prrc2b	A	C	2: 32,102,297 (GRCm39)	H790P	possibly damaging	Het
Rest	T	G	5: 77,429,027 (GRCm39)	V482G	probably benign	Het
Ros1	T	A	10: 52,039,999 (GRCm39)	I322L	probably benign	Het
Rpl18	G	A	7: 45,369,554 (GRCm39)		probably benign	Het
Rsad2	G	A	12: 26,500,577 (GRCm39)	H237Y	probably benign	Het
Sbf2	A	G	7: 110,040,741 (GRCm39)	L362S	probably damaging	Het
Sgsm3	A	G	15: 80,893,464 (GRCm39)	T409A	probably benign	Het
Slc16a14	T	C	1: 84,890,430 (GRCm39)	T292A	possibly damaging	Het
Spg11	T	G	2: 121,927,837 (GRCm39)	K649T	probably damaging	Het
Srprb	T	A	9: 103,067,415 (GRCm39)	I268F	possibly damaging	Het
Tap2	G	A	17: 34,433,683 (GRCm39)	G566S	probably damaging	Het
Tecpr2	T	C	12: 110,885,325 (GRCm39)	V168A	possibly damaging	Het
Tln1	G	C	4: 43,555,052 (GRCm39)	F259L	probably damaging	Het
Tmem181a	T	A	17: 6,330,890 (GRCm39)	L50H	probably damaging	Het
Tshz1	A	G	18: 84,032,194 (GRCm39)	I738T	probably damaging	Het
Tspan12	T	C	6: 21,772,637 (GRCm39)	E304G	probably benign	Het
Urb1	C	T	16: 90,559,271 (GRCm39)	G1671S	probably damaging	Het
Vmn2r2	T	C	3: 64,024,782 (GRCm39)	S600G	possibly damaging	Het
Xpo7	T	C	14: 70,921,159 (GRCm39)	Y603C	possibly damaging	Het
Zfp457	G	A	13: 67,442,015 (GRCm39)	H91Y	probably damaging	Het
Zfp664	T	A	5: 124,963,250 (GRCm39)	C215S	unknown	Het
Zfp882	G	A	8: 72,668,349 (GRCm39)	G392D	probably benign	Het

Other mutations in Wnt3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01294:Wnt3	APN	11	103,699,140 (GRCm39)	missense	possibly damaging	0.81
IGL01645:Wnt3	APN	11	103,703,204 (GRCm39)	missense	probably benign	0.00
IGL01989:Wnt3	APN	11	103,703,233 (GRCm39)	missense	probably benign	0.44
IGL02087:Wnt3	APN	11	103,703,185 (GRCm39)	missense	probably benign	0.34
IGL02525:Wnt3	APN	11	103,703,296 (GRCm39)	missense	probably damaging	1.00
R0494:Wnt3	UTSW	11	103,703,141 (GRCm39)	missense	probably damaging	1.00
R0615:Wnt3	UTSW	11	103,703,207 (GRCm39)	missense	possibly damaging	0.68
R1438:Wnt3	UTSW	11	103,699,077 (GRCm39)	missense	probably damaging	1.00
R2058:Wnt3	UTSW	11	103,703,111 (GRCm39)	missense	probably damaging	0.97
R2127:Wnt3	UTSW	11	103,703,474 (GRCm39)	missense	possibly damaging	0.82
R2128:Wnt3	UTSW	11	103,703,474 (GRCm39)	missense	possibly damaging	0.82
R4470:Wnt3	UTSW	11	103,703,450 (GRCm39)	missense	probably damaging	0.99
R4878:Wnt3	UTSW	11	103,699,031 (GRCm39)	missense	possibly damaging	0.88
R5616:Wnt3	UTSW	11	103,703,596 (GRCm39)	critical splice donor site	probably null
R6472:Wnt3	UTSW	11	103,699,100 (GRCm39)	missense	possibly damaging	0.89
R6687:Wnt3	UTSW	11	103,703,411 (GRCm39)	missense	probably damaging	1.00
R7652:Wnt3	UTSW	11	103,703,290 (GRCm39)	missense	possibly damaging	0.83
R7760:Wnt3	UTSW	11	103,702,266 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTGTCCCCAAGGAAAGTCAG -3'
(R):5'- TTGTCCAAGACAGGCCCAAAG -3'

Sequencing Primer
(F):5'- GTCCCCAAGGAAAGTCAGAAAGC -3'
(R):5'- AAGATGGCCAGGCTGTCATCTATG -3'

Posted On

2017-07-14