Mutagenetix > Incidental Mutation

Incidental Mutation 'R6053:Zfp169'

484263

Institutional Source

Beutler Lab

Gene Symbol

Zfp169

Ensembl Gene

ENSMUSG00000050954

Gene Name

zinc finger protein 169

Synonyms

MMRRC Submission

044221-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6053 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48487647-48513451 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 48498858 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 28 (W28R)

Ref Sequence

ENSEMBL: ENSMUSP00000135520 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110110] [ENSMUST00000167682] [ENSMUST00000176176] [ENSMUST00000176949] [ENSMUST00000176996] [ENSMUST00000177530]

AlphaFold

E9Q3R6

Predicted Effect

unknown
Transcript: ENSMUST00000110110
AA Change: W28R

SMART Domains

Protein: ENSMUSP00000105737
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART
ZnF_C2H2	257	279	9.08e-4	SMART
ZnF_C2H2	285	308	2.2e-2	SMART
ZnF_C2H2	314	336	9.73e-4	SMART
ZnF_C2H2	342	364	2.86e-1	SMART
ZnF_C2H2	370	392	4.72e-2	SMART
ZnF_C2H2	398	420	4.24e-4	SMART
ZnF_C2H2	426	448	1.13e-4	SMART
ZnF_C2H2	454	476	2.2e-2	SMART
ZnF_C2H2	482	504	2.99e-4	SMART
ZnF_C2H2	510	532	2.57e-3	SMART
ZnF_C2H2	539	561	3.44e-4	SMART
ZnF_C2H2	567	589	3.69e-4	SMART
ZnF_C2H2	595	617	8.02e-5	SMART
ZnF_C2H2	623	645	1.26e-2	SMART
ZnF_C2H2	651	673	4.79e-3	SMART
ZnF_C2H2	679	701	1.3e-4	SMART
ZnF_C2H2	707	729	5.5e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000167682
AA Change: W28R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127591
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176176
AA Change: W28R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134793
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176949
AA Change: W28R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135695
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176996
AA Change: W28R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135520
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177474

Predicted Effect

unknown
Transcript: ENSMUST00000177530
AA Change: W28R

SMART Domains

Protein: ENSMUSP00000135414
Gene: ENSMUSG00000050954
AA Change: W28R

Domain	Start	End	E-Value	Type
KRAB	14	74	9.74e-36	SMART
ZnF_C2H2	257	279	9.08e-4	SMART
ZnF_C2H2	285	308	2.2e-2	SMART
ZnF_C2H2	314	336	9.73e-4	SMART
ZnF_C2H2	342	364	2.86e-1	SMART
ZnF_C2H2	370	392	4.72e-2	SMART
ZnF_C2H2	398	420	4.24e-4	SMART
ZnF_C2H2	426	448	1.13e-4	SMART
ZnF_C2H2	454	476	2.2e-2	SMART
ZnF_C2H2	482	504	2.99e-4	SMART
ZnF_C2H2	510	532	2.57e-3	SMART
ZnF_C2H2	539	561	3.44e-4	SMART
ZnF_C2H2	567	589	3.69e-4	SMART
ZnF_C2H2	595	617	8.02e-5	SMART
ZnF_C2H2	623	645	1.26e-2	SMART
ZnF_C2H2	651	673	4.79e-3	SMART
ZnF_C2H2	679	701	1.3e-4	SMART
ZnF_C2H2	707	729	5.5e-3	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 94.9%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous disruption of this locus does not result in an overt phenotype early in life. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	A	G	15: 8,188,461	D785G	probably benign	Het
Abca4	T	C	3: 122,171,017	Y2158H	probably damaging	Het
Acap1	T	G	11: 69,887,070		probably null	Het
Adamts4	T	A	1: 171,252,715	I279N	possibly damaging	Het
Akap3	A	G	6: 126,866,533	E705G	probably damaging	Het
Arap3	C	A	18: 37,990,771	L398F	probably damaging	Het
BC067074	C	A	13: 113,320,726	P1102Q	possibly damaging	Het
Catsperg1	A	T	7: 29,210,814	L34*	probably null	Het
Ccdc171	T	G	4: 83,795,219	L1165R	probably damaging	Het
Ccdc3	G	A	2: 5,229,027	V221I	probably benign	Het
Cdc42ep4	T	A	11: 113,728,986	H193L	probably damaging	Het
Clcn1	G	A	6: 42,300,274	W361*	probably null	Het
Cntn2	T	C	1: 132,518,352	N832S	probably benign	Het
Col5a1	A	T	2: 28,014,377		probably benign	Het
Ctc1	T	A	11: 69,027,901	M550K	probably benign	Het
Dennd5a	T	C	7: 109,933,745	R273G	probably damaging	Het
Dnajb7	T	C	15: 81,407,299	E279G	probably benign	Het
Dopey1	G	T	9: 86,515,294	G882W	possibly damaging	Het
Dvl2	A	G	11: 70,005,993	R238G	possibly damaging	Het
Ect2l	A	T	10: 18,141,844	L629Q	probably damaging	Het
Enpp1	T	C	10: 24,657,126	D497G	possibly damaging	Het
Ep400	T	C	5: 110,755,795	M313V	probably benign	Het
Epn2	T	C	11: 61,546,497	Y83C	probably damaging	Het
Ercc3	T	G	18: 32,246,754	V338G	probably damaging	Het
Fkbp5	T	C	17: 28,428,466	I132V	probably benign	Het
Fn1	A	T	1: 71,599,290	Y1846N	probably damaging	Het
Foxg1	T	C	12: 49,385,378	L298P	possibly damaging	Het
Foxo3	G	A	10: 42,197,214	P436S	probably benign	Het
Gbx2	T	C	1: 89,930,437	T107A	probably benign	Het
Gli1	G	T	10: 127,334,315	H324N	probably damaging	Het
Gnas	T	A	2: 174,299,852	S605T	possibly damaging	Het
Ica1	C	T	6: 8,630,783	A431T	probably benign	Het
Il10ra	C	T	9: 45,256,303	D319N	probably damaging	Het
Jmjd4	T	A	11: 59,454,044	H274Q	probably damaging	Het
Krt28	C	A	11: 99,371,201	L294F	probably benign	Het
Ltbp3	C	A	19: 5,752,094	T766K	probably damaging	Het
Man2b2	C	A	5: 36,813,038	M841I	probably benign	Het
Mmp14	T	A	14: 54,435,890	M85K	probably benign	Het
Mmp1b	T	A	9: 7,385,031	D206V	probably benign	Het
Muc5b	T	C	7: 141,864,708	F3797S	probably benign	Het
Mug1	A	G	6: 121,865,738	D561G	probably benign	Het
Myo18a	T	A	11: 77,818,176	H471Q	probably damaging	Het
Nfkb2	A	G	19: 46,311,812	E873G	probably damaging	Het
Ngfr	G	A	11: 95,571,006	H361Y	possibly damaging	Het
Nnt	T	C	13: 119,357,509	T679A	possibly damaging	Het
Notch1	A	T	2: 26,472,912	N947K	probably benign	Het
Nrg4	A	T	9: 55,236,490	V94D	probably benign	Het
Nsd1	G	T	13: 55,293,609	C1631F	probably damaging	Het
Nutm1	T	C	2: 112,249,090	T827A	probably benign	Het
Olfr1219	T	C	2: 89,074,554	D179G	probably damaging	Het
Olfr187	A	T	16: 59,035,988	Y250N	probably damaging	Het
Olfr328	T	A	11: 58,552,066	M58L	possibly damaging	Het
Olfr414	C	A	1: 174,430,569	S47*	probably null	Het
Olfr883	TTGCTGT	TTGCTGTCTGCTGT	9: 38,026,541		probably null	Het
Orai3	C	T	7: 127,773,878	P184S	probably benign	Het
Paqr3	A	T	5: 97,111,278	S56T	probably benign	Het
Pard6b	C	T	2: 168,099,053	T320M	possibly damaging	Het
Pgs1	T	C	11: 118,001,709	S166P	probably damaging	Het
Plaa	T	C	4: 94,589,884	T114A	probably benign	Het
Plekha6	G	C	1: 133,272,307	R208P	possibly damaging	Het
Plin4	A	T	17: 56,108,618	D73E	probably benign	Het
Plxnb1	T	C	9: 109,111,707	L1550P	probably damaging	Het
Rbak	A	C	5: 143,174,682	Y205*	probably null	Het
Rgs12	T	C	5: 34,965,952	F360L	probably benign	Het
Rgs22	A	T	15: 36,100,007	D187E	probably benign	Het
Sec24d	C	T	3: 123,279,222	Q66*	probably null	Het
Sh2d5	T	A	4: 138,255,562	M85K	probably damaging	Het
Sos1	A	G	17: 80,415,034	V861A	possibly damaging	Het
Stk11ip	T	A	1: 75,534,255		probably null	Het
Svs1	A	G	6: 48,988,488	T477A	probably benign	Het
Tmem26	G	A	10: 68,748,484	E127K	probably benign	Het
Tmem41a	T	G	16: 21,934,989	T211P	possibly damaging	Het
Tmem69	T	A	4: 116,553,384	M130L	possibly damaging	Het
Trak2	C	A	1: 58,904,069	R726L	possibly damaging	Het
Vmn1r231	A	G	17: 20,889,819	I278T	probably damaging	Het
Vwf	G	A	6: 125,600,665	V490I	probably benign	Het
Wdr33	C	T	18: 31,878,063	T255I	possibly damaging	Het
Ylpm1	G	T	12: 84,996,503	W5L	possibly damaging	Het

Other mutations in Zfp169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01925:Zfp169	APN	13	48490763	unclassified	probably benign
IGL03329:Zfp169	APN	13	48490794	unclassified	probably benign
IGL03382:Zfp169	APN	13	48491163	unclassified	probably benign
IGL03394:Zfp169	APN	13	48489924	missense	possibly damaging	0.93
BB010:Zfp169	UTSW	13	48490481	missense	unknown
BB020:Zfp169	UTSW	13	48490481	missense	unknown
R0571:Zfp169	UTSW	13	48489690	missense	possibly damaging	0.71
R1714:Zfp169	UTSW	13	48498854	missense	probably benign	0.35
R1784:Zfp169	UTSW	13	48489819	missense	possibly damaging	0.61
R3108:Zfp169	UTSW	13	48489996	missense	possibly damaging	0.86
R3689:Zfp169	UTSW	13	48506901	splice site	probably benign
R4444:Zfp169	UTSW	13	48490337	missense	possibly damaging	0.94
R4665:Zfp169	UTSW	13	48490863	unclassified	probably benign
R4719:Zfp169	UTSW	13	48490158	missense	probably benign	0.06
R4745:Zfp169	UTSW	13	48490232	missense	possibly damaging	0.71
R5288:Zfp169	UTSW	13	48490275	missense	possibly damaging	0.61
R5384:Zfp169	UTSW	13	48490275	missense	possibly damaging	0.61
R5979:Zfp169	UTSW	13	48491040	unclassified	probably benign
R6823:Zfp169	UTSW	13	48490996	unclassified	probably benign
R7084:Zfp169	UTSW	13	48498863	missense	probably benign	0.10
R7679:Zfp169	UTSW	13	48498383	missense	probably damaging	0.99
R7933:Zfp169	UTSW	13	48490481	missense	unknown
R8298:Zfp169	UTSW	13	48498377	nonsense	probably null
R8322:Zfp169	UTSW	13	48491099	missense	unknown
R9047:Zfp169	UTSW	13	48498816	missense	probably damaging	1.00
R9124:Zfp169	UTSW	13	48491081	missense	unknown
R9126:Zfp169	UTSW	13	48491081	missense	unknown
R9131:Zfp169	UTSW	13	48491081	missense	unknown
R9132:Zfp169	UTSW	13	48491081	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GTTCACTGCAAAACATGGACAC -3'
(R):5'- AAACTGGCGTCCATACAAGGG -3'

Sequencing Primer
(F):5'- TGCAAAACATGGACACTCTAAAG -3'
(R):5'- TGGCGTCCATACAAGGGAGTTG -3'

Posted On

2017-07-14